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The map label for this gene is groEL [H]

Identifier: 15594994

GI number: 15594994

Start: 688490

End: 690127

Strand: Direct

Name: groEL [H]

Synonym: BB0649

Alternate gene names: 15594994

Gene position: 688490-690127 (Clockwise)

Preceding gene: 15594990

Following gene: 15594995

Centisome position: 75.6

GC content: 36.39

Gene sequence:

>1638_bases
ATGGCTAAAGACATATATTTTAATGAGGATGCTAGAAAAAGCTTACTTAGTGGCGTTGAAAAATTATCCAATGCTGTAAA
AGTAACTCTTGGGCCAAAAGGGAGAAATGTCCTTATTGATAAAAAGTTCGGCTCTCCAACGGTTACAAAGGATGGGGTTA
GCGTTGCTCGTGAGATTGAGCTTGAAAATCCGTTTGAAAACATGGGGGCACAGCTTTTAAAGGAAGTTGCTATTAAAACA
AATGATGTTGCTGGTGATGGAACAACAACTGCTACTGTTCTTGCTTATGCTATTGCAAGAGAAGGCCTTAAGAATGTGTC
TTCAGGAATCAATCCTATTGGAATAAAAAAGGGAATAGATCACGCTGTAAATTTGGCTGCTGAGAAAATTCGTCAGTCTG
CAAAAAAGATTACAACAAAAGAAGAGATTGCACAAGTAGCTTCAATTTCTGCTAATAATGACAGTTATATAGGTGAAAAA
ATTGCTGAGGCAATGGATAAAGTTGGAAAAGATGGTGTTATAACAGTTGAAGAGTCAAAAACCTTTGATACTACGATTTC
TTATGTTGAGGGTATGCAATTTGATAGAGGATATCTTTCTCCTTATTTTTCTACCAATAAAGAAAATATGAGTGTTAATT
TTGACGATGCTTTCATATTGATATATGAGAAAAAGATTAGTTCTATTAAAGAGCTTTTACCAGTTCTTGAGAAAGTTTTA
GGGACAAATAAACCTTTATTAATTATTGCTGAGGATATTGAGGGGGATGCTCTTGCTGCTCTTGTTTTAAACAGCGTTAG
AGGAGCTTTAAAGGTATGTGCAATTAAATCTCCTGGTTTTGGTGATAGACGAAAAGCAATGCTTGAGGATATTGCAGTGC
TTACCGGCGGTGTTTTAATCAGTGAGGAGCTAGGCCTTACTCTTGAGACAGTTGAAATTGAGCAACTTGGACAGGCTAAA
ACTATTAAGGTTGATAAAGACAATACCACTATTATTAATACCGGCAATAAAGAGCAAATAAAGGAGCGTTCAGAGCTTAT
TAAAAAGCAAATTGAAGATTCAACATCTGAATATGATAAAGAAAAACTTCAAGAGCGTCTTGCAAAACTTGTTGGCGGAG
TTGCTGTTATTAATGTTGGAGCTGTTACTGAGGTAGAGCTTAAGGAGAAAAAGCATAGAGTTGAGGACGCTCTTTCTGCA
ACTCGTGCTGCTGTTGAAGAGGGTGTTGTGCCTGGCGGTGGATCAACTCTTATTGAAGTTGCTATGTATTTAGATACAAT
AGATACAAGTAAATTAAGCTATGAGGAAAAGCAAGGTTTTGAGATTGTAAAAAGAAGTCTTGAAGAGCCAATGAGACAGA
TTATTTCAAATGCTGGTTTTGAAGGATCTATTTATATTCATCAAATTAAAACAGAAAAAAAAGGGCTTGGGTTTGATGCT
TCCAGCTTTAAGTGGGTAAATATGATTGAGAGTGGAATAATTGATCCTGCTAAGGTTACAAGAAGCGCGCTTCAAAATGC
TGCTTCAATTGCTGGACTTTTATTAACAACAGAATGTGCAATCACAGATATTAAAGAAGAGAAAAATACTTCTGGTGGTG
GTGGTTATCCTATGGACCCAGGAATGGGAATGATGTAA

Upstream 100 bases:

>100_bases
ATAGAAGTAATACGCTACTATAGCGAATGTATTAAGTTTATGTTAATCTTATATTGTTATATGTAAATTATTTTTATTTA
TAGAAAGGGGTAAAATTTTT

Downstream 100 bases:

>100_bases
ATTAAAGTTTCACCGGCAAGCTTGTGTTTTTGCCGGTGAATATTTTATTATTCAAAGGAATTATTTTGAGTGAGGATGAA
TTTGTTTTTTGTATAGGCTA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAKDIYFNEDARKSLLSGVEKLSNAVKVTLGPKGRNVLIDKKFGSPTVTKDGVSVAREIELENPFENMGAQLLKEVAIKT
NDVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKKGIDHAVNLAAEKIRQSAKKITTKEEIAQVASISANNDSYIGEK
IAEAMDKVGKDGVITVEESKTFDTTISYVEGMQFDRGYLSPYFSTNKENMSVNFDDAFILIYEKKISSIKELLPVLEKVL
GTNKPLLIIAEDIEGDALAALVLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGGVLISEELGLTLETVEIEQLGQAK
TIKVDKDNTTIINTGNKEQIKERSELIKKQIEDSTSEYDKEKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSA
TRAAVEEGVVPGGGSTLIEVAMYLDTIDTSKLSYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKTEKKGLGFDA
SSFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITDIKEEKNTSGGGGYPMDPGMGMM

Sequences:

>Translated_545_residues
MAKDIYFNEDARKSLLSGVEKLSNAVKVTLGPKGRNVLIDKKFGSPTVTKDGVSVAREIELENPFENMGAQLLKEVAIKT
NDVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKKGIDHAVNLAAEKIRQSAKKITTKEEIAQVASISANNDSYIGEK
IAEAMDKVGKDGVITVEESKTFDTTISYVEGMQFDRGYLSPYFSTNKENMSVNFDDAFILIYEKKISSIKELLPVLEKVL
GTNKPLLIIAEDIEGDALAALVLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGGVLISEELGLTLETVEIEQLGQAK
TIKVDKDNTTIINTGNKEQIKERSELIKKQIEDSTSEYDKEKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSA
TRAAVEEGVVPGGGSTLIEVAMYLDTIDTSKLSYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKTEKKGLGFDA
SSFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITDIKEEKNTSGGGGYPMDPGMGMM
>Mature_544_residues
AKDIYFNEDARKSLLSGVEKLSNAVKVTLGPKGRNVLIDKKFGSPTVTKDGVSVAREIELENPFENMGAQLLKEVAIKTN
DVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKKGIDHAVNLAAEKIRQSAKKITTKEEIAQVASISANNDSYIGEKI
AEAMDKVGKDGVITVEESKTFDTTISYVEGMQFDRGYLSPYFSTNKENMSVNFDDAFILIYEKKISSIKELLPVLEKVLG
TNKPLLIIAEDIEGDALAALVLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGGVLISEELGLTLETVEIEQLGQAKT
IKVDKDNTTIINTGNKEQIKERSELIKKQIEDSTSEYDKEKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSAT
RAAVEEGVVPGGGSTLIEVAMYLDTIDTSKLSYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKTEKKGLGFDAS
SFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITDIKEEKNTSGGGGYPMDPGMGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=50.0941619585687, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=50.0941619585687, Blast_Score=508, Evalue=1e-144,
Organism=Escherichia coli, GI1790586, Length=524, Percent_Identity=59.1603053435115, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17555558, Length=531, Percent_Identity=47.4576271186441, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=48.1132075471698, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=52.851711026616, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322524, Length=129, Percent_Identity=32.5581395348837, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=48.8636363636364, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=48.8636363636364, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI45550936, Length=529, Percent_Identity=46.3137996219282, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI45550132, Length=529, Percent_Identity=46.3137996219282, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI45550935, Length=529, Percent_Identity=46.3137996219282, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864606, Length=554, Percent_Identity=41.5162454873646, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24584129, Length=525, Percent_Identity=36.3809523809524, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI19921262, Length=525, Percent_Identity=36.3809523809524, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI24583944, Length=169, Percent_Identity=24.8520710059172, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58952; Mature: 58821

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDIYFNEDARKSLLSGVEKLSNAVKVTLGPKGRNVLIDKKFGSPTVTKDGVSVAREIE
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCC
LENPFENMGAQLLKEVAIKTNDVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKKGID
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
HAVNLAAEKIRQSAKKITTKEEIAQVASISANNDSYIGEKIAEAMDKVGKDGVITVEESK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TFDTTISYVEGMQFDRGYLSPYFSTNKENMSVNFDDAFILIYEKKISSIKELLPVLEKVL
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHH
GTNKPLLIIAEDIEGDALAALVLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGGVLI
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCEEE
SEELGLTLETVEIEQLGQAKTIKVDKDNTTIINTGNKEQIKERSELIKKQIEDSTSEYDK
ECCCCCEEEEEEHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSATRAAVEEGVVPGGGSTLIEV
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AMYLDTIDTSKLSYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKTEKKGLGFDA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCC
SSFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITDIKEEKNTSGGGGYPMDP
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GMGMM
CCCCC
>Mature Secondary Structure 
AKDIYFNEDARKSLLSGVEKLSNAVKVTLGPKGRNVLIDKKFGSPTVTKDGVSVAREIE
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHCC
LENPFENMGAQLLKEVAIKTNDVAGDGTTTATVLAYAIAREGLKNVSSGINPIGIKKGID
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
HAVNLAAEKIRQSAKKITTKEEIAQVASISANNDSYIGEKIAEAMDKVGKDGVITVEESK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TFDTTISYVEGMQFDRGYLSPYFSTNKENMSVNFDDAFILIYEKKISSIKELLPVLEKVL
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHH
GTNKPLLIIAEDIEGDALAALVLNSVRGALKVCAIKSPGFGDRRKAMLEDIAVLTGGVLI
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCEEE
SEELGLTLETVEIEQLGQAKTIKVDKDNTTIINTGNKEQIKERSELIKKQIEDSTSEYDK
ECCCCCEEEEEEHHHCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLVGGVAVINVGAVTEVELKEKKHRVEDALSATRAAVEEGVVPGGGSTLIEV
HHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
AMYLDTIDTSKLSYEEKQGFEIVKRSLEEPMRQIISNAGFEGSIYIHQIKTEKKGLGFDA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCC
SSFKWVNMIESGIIDPAKVTRSALQNAASIAGLLLTTECAITDIKEEKNTSGGGGYPMDP
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GMGMM
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA