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The map label for this gene is dacD [H]

Identifier: 15594950

GI number: 15594950

Start: 630769

End: 631986

Strand: Direct

Name: dacD [H]

Synonym: BB0605

Alternate gene names: 15594950

Gene position: 630769-631986 (Clockwise)

Preceding gene: 15594947

Following gene: 15594951

Centisome position: 69.26

GC content: 27.34

Gene sequence:

>1218_bases
ATGAATAGTATCTATGTTATTGGGAAATTGTTATTAACTTTATTTTTAATTTTTTTCCCGTTTTGTTATAATCTTTTTGC
AGTTAATTTAGCTGAGATTAATAAATTATCAGAGTATGCAAAGTCAATAGTTTTAATAGATTTTGATACTAAGCGAATAC
TTTATTCTAAGAAGCCCAATTTGGTTTTTCCTCCAGCATCTCTTACAAAGATTGTTACAATTTATACAGCTTTAATTGAA
GCTGAAAAGCGAAATATAAAATTAAAAAGCATAGTTCCTATTAGCGATTCTGCTTCATATTATAATGCACCCCCCAATTC
TTCTTTGATGTTTTTAGAAAAAGGTCAAATTGTTAATTTTGAAGAGATTTTAAAAGGACTTTCAGTTTCTTCGGGTAATG
ATTCTTCTATTGCAATTGCTGAGTTTGTAGTAGGCAATTTAAATAGCTTTGTTAATTTAATGAATATTAATGTTTTAAAT
TTAGGGCTTTTTAATATGCATTTTGTTGAACCTTCTGGATATAGCAGCGAGAATAAGATTACAGCACTAGATATGGCTTT
TTTTGTGAAATCTTATATAGAAAAGTTTAAATTTATGCTTAATATTCATTCTTTAAAGTATTTTATTTATCCAAAGAGTA
GAAATTTAGGAACTGCTTTGTCATCAAAATTTTTAAACTTAAAACAAAGAAATGCTAATTTATTAATATATGATTACCCT
TATTCAGATGGCATTAAAACGGGATATATTAAGGAATCAGGCTTAAATCTTGTTGCTACTGCTAAAAAGGGTGAGAGAAG
ATTAATAGCAGTTGTATTGGGGGTTGAAAAAGGAATTAATGGATTTGGAGAGAAAATGAGATCTTCGATTGCAAAAAATT
TATTTGAATATGGATTTAATAAATATTCTAAATTTCCTTTAATAGTAAAATTAAAAGAAAAAGTCTATAATGGTACAGTG
GATACAGTTGCTCTTTTTTCTAAAGAGCCTTTTTATTATATTTTAACTAAAGATGAATTTGATAAAATTAATATAAGTTA
TACTGTTGATAAATTGGTTGCTCCACTTAGTGGGGATATGCCTGTTGGGAGGGCTATGATTTTTTTAGAAAATGAAAAAA
TAGGGGATGTTGCTTTGTTTAGTGGCAAGGTAAAAAGATTAGGGTTTTGGCAAGGTCTTTATAAGAGTTTTATAAATCTT
TTTTCAAGAGAGTATTAA

Upstream 100 bases:

>100_bases
CAAAGCTGTTATAAAATCATAAGAATTCATAAAAATGGCTCCTTTTTAATTGTATTGTAATTAACAATCAATAAATAATA
TATAATTTTAATATTACAAC

Downstream 100 bases:

>100_bases
TTTTATGTTAAATCATTTTAATTTTAAATTAAAACGCGATGTTACAATAATAGTTCCTGGTGAAGCTTTTGTTTCAAACA
AAAGAGTGATTTCTACAATT

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNSIYVIGKLLLTLFLIFFPFCYNLFAVNLAEINKLSEYAKSIVLIDFDTKRILYSKKPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPPNSSLMFLEKGQIVNFEEILKGLSVSSGNDSSIAIAEFVVGNLNSFVNLMNINVLN
LGLFNMHFVEPSGYSSENKITALDMAFFVKSYIEKFKFMLNIHSLKYFIYPKSRNLGTALSSKFLNLKQRNANLLIYDYP
YSDGIKTGYIKESGLNLVATAKKGERRLIAVVLGVEKGINGFGEKMRSSIAKNLFEYGFNKYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYILTKDEFDKINISYTVDKLVAPLSGDMPVGRAMIFLENEKIGDVALFSGKVKRLGFWQGLYKSFINL
FSREY

Sequences:

>Translated_405_residues
MNSIYVIGKLLLTLFLIFFPFCYNLFAVNLAEINKLSEYAKSIVLIDFDTKRILYSKKPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPPNSSLMFLEKGQIVNFEEILKGLSVSSGNDSSIAIAEFVVGNLNSFVNLMNINVLN
LGLFNMHFVEPSGYSSENKITALDMAFFVKSYIEKFKFMLNIHSLKYFIYPKSRNLGTALSSKFLNLKQRNANLLIYDYP
YSDGIKTGYIKESGLNLVATAKKGERRLIAVVLGVEKGINGFGEKMRSSIAKNLFEYGFNKYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYILTKDEFDKINISYTVDKLVAPLSGDMPVGRAMIFLENEKIGDVALFSGKVKRLGFWQGLYKSFINL
FSREY
>Mature_405_residues
MNSIYVIGKLLLTLFLIFFPFCYNLFAVNLAEINKLSEYAKSIVLIDFDTKRILYSKKPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPPNSSLMFLEKGQIVNFEEILKGLSVSSGNDSSIAIAEFVVGNLNSFVNLMNINVLN
LGLFNMHFVEPSGYSSENKITALDMAFFVKSYIEKFKFMLNIHSLKYFIYPKSRNLGTALSSKFLNLKQRNANLLIYDYP
YSDGIKTGYIKESGLNLVATAKKGERRLIAVVLGVEKGINGFGEKMRSSIAKNLFEYGFNKYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYILTKDEFDKINISYTVDKLVAPLSGDMPVGRAMIFLENEKIGDVALFSGKVKRLGFWQGLYKSFINL
FSREY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=28.125, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786851, Length=345, Percent_Identity=31.0144927536232, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787062, Length=381, Percent_Identity=29.3963254593176, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87082054, Length=218, Percent_Identity=29.3577981651376, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45836; Mature: 45836

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIYVIGKLLLTLFLIFFPFCYNLFAVNLAEINKLSEYAKSIVLIDFDTKRILYSKKPN
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCC
LVFPPASLTKIVTIYTALIEAEKRNIKLKSIVPISDSASYYNAPPNSSLMFLEKGQIVNF
EEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCEECH
EEILKGLSVSSGNDSSIAIAEFVVGNLNSFVNLMNINVLNLGLFNMHFVEPSGYSSENKI
HHHHHCCCCCCCCCCCEEHHHHHHHCHHHHHHHHEEEEEEEEEEEEEEECCCCCCCCCCE
TALDMAFFVKSYIEKFKFMLNIHSLKYFIYPKSRNLGTALSSKFLNLKQRNANLLIYDYP
EEHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
YSDGIKTGYIKESGLNLVATAKKGERRLIAVVLGVEKGINGFGEKMRSSIAKNLFEYGFN
CCCCCCCCEEECCCCEEEEECCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCC
KYSKFPLIVKLKEKVYNGTVDTVALFSKEPFYYILTKDEFDKINISYTVDKLVAPLSGDM
CCCCCCEEEEEHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEHHHHHCCCCCCC
PVGRAMIFLENEKIGDVALFSGKVKRLGFWQGLYKSFINLFSREY
CCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSIYVIGKLLLTLFLIFFPFCYNLFAVNLAEINKLSEYAKSIVLIDFDTKRILYSKKPN
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCC
LVFPPASLTKIVTIYTALIEAEKRNIKLKSIVPISDSASYYNAPPNSSLMFLEKGQIVNF
EEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCEECH
EEILKGLSVSSGNDSSIAIAEFVVGNLNSFVNLMNINVLNLGLFNMHFVEPSGYSSENKI
HHHHHCCCCCCCCCCCEEHHHHHHHCHHHHHHHHEEEEEEEEEEEEEEECCCCCCCCCCE
TALDMAFFVKSYIEKFKFMLNIHSLKYFIYPKSRNLGTALSSKFLNLKQRNANLLIYDYP
EEHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
YSDGIKTGYIKESGLNLVATAKKGERRLIAVVLGVEKGINGFGEKMRSSIAKNLFEYGFN
CCCCCCCCEEECCCCEEEEECCCCCCEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCC
KYSKFPLIVKLKEKVYNGTVDTVALFSKEPFYYILTKDEFDKINISYTVDKLVAPLSGDM
CCCCCCEEEEEHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEHHHHHCCCCCCC
PVGRAMIFLENEKIGDVALFSGKVKRLGFWQGLYKSFINLFSREY
CCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]