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The map label for this gene is glyA [H]

Identifier: 15594946

GI number: 15594946

Start: 625007

End: 626260

Strand: Direct

Name: glyA [H]

Synonym: BB0601

Alternate gene names: 15594946

Gene position: 625007-626260 (Clockwise)

Preceding gene: 15594945

Following gene: 15594947

Centisome position: 68.63

GC content: 34.21

Gene sequence:

>1254_bases
ATGAGAGATGATCAAATATTTAATTTAATTGAAAAGGAAAAATTAAGAGAAAGAGAGCATATTGAACTTATTGCTTCTGA
AAATTTTACATCTTTAGAGATAAGGCAGGCTGTTGGTAGTATTTTAACTAATAAGTATGCCGAAGGATATCCTTTGAATC
GATACTACGGTGGTTGTTCTTTTATTGATGAGATTGAAACTCTGGCAATTTCGAGAGCAAAAGAGCTTTTTGGCGCAAAG
TATGCCAATGTTCAACCTCATAGCGGATCTCAGGCCAATATGGCTGCCATAATGGCTCTTATTAGCCCGGGTGACAGGAT
TCTTGGTATGCAATTATCTCATGGAGGGCATTTAACTCACGGCAGCAGGGTAAATTTTTCTGGTATATTTTTTAACACTT
ATTTTTATGGTGTTTCTAGAGATTCTGAGCTAATTGATTATGATGAAGTTCTTAAAATAGCTAAAGATTGCAGGCCAAAT
TTAATAATAGCTGGAGCTTCTTCTTATTCAAGAGAAATTGATTTTAAAAAATTTAGAGAAATAGCAGATGATGTTTCTGC
TTATCTTTTGTGTGATATTGCTCATATTGCAGGCCTTATTGTTGCCGGTTTTCATAATTCCTCAATTGATGTGGCGCATC
TTACTACAAGTACTACGCATAAAACTTTAAGAGGGCCAAGAGGTGGAATAATACTTTCTGGAAAGGATTTTGACAAATTA
GTAAACTTTAATGGAAAAGAGAAGCCTTTGTTTAATGCTGTAAATTCTACAGTTTTTCCTGGAACTCAAGGGGGTCCTTT
AGTTCATGTTATTGCGGGTAAGGCTATTGCATTCAAAGAAGCTCTTCAAGAAAGTTTTAAAGAATACATTGCTAACGTAA
TAAAAAATACTAAAGTTATGGCTGAATATTTCAAATCGGAAGGATTTCGTATTGTTAGTGGGGGCACAGACAATCATTTG
TTTTTGGTTGATCTTAGTAGTTCGGATCTCACCGGTGCTGATGCTGAGAAATTACTTGAGAGCGTAAATATTACTTTAAA
TAAAAATGCTATTCCTTTTGATAAAAAAAGCCCTTCTTTGGCTTCTGGTATTAGAATTGGGGGCGCTGCTATTACTTCTA
GAGGCCTAAATGAAAGCGATTCTTTAAATGTTGCTAAATTTATTGTTAGAGCTTTAAAGGCAAAGTCTGATATTGAATTA
AAACAAATAAAAAAGGAAGTTGTAAGATTTATTAGAGATTTTGACATGCCTTGA

Upstream 100 bases:

>100_bases
TTTTTGATGGATATTTAGATTCTGGGTTTTTAAAGTATAAAAGCTTGACAAACGGATCGTATTCTTTAATGCATAGGTTT
GTTTTGGAGGATAATTTTTA

Downstream 100 bases:

>100_bases
TTACGCTGGAGGTTATGATCAATAAATGCCAAGCCCAATTAGAGTGTTTTTTTTAGTGTTGTTGTTTATTTTTATTTTTA
ATCCCGTTTTAATAGCAATG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MRDDQIFNLIEKEKLREREHIELIASENFTSLEIRQAVGSILTNKYAEGYPLNRYYGGCSFIDEIETLAISRAKELFGAK
YANVQPHSGSQANMAAIMALISPGDRILGMQLSHGGHLTHGSRVNFSGIFFNTYFYGVSRDSELIDYDEVLKIAKDCRPN
LIIAGASSYSREIDFKKFREIADDVSAYLLCDIAHIAGLIVAGFHNSSIDVAHLTTSTTHKTLRGPRGGIILSGKDFDKL
VNFNGKEKPLFNAVNSTVFPGTQGGPLVHVIAGKAIAFKEALQESFKEYIANVIKNTKVMAEYFKSEGFRIVSGGTDNHL
FLVDLSSSDLTGADAEKLLESVNITLNKNAIPFDKKSPSLASGIRIGGAAITSRGLNESDSLNVAKFIVRALKAKSDIEL
KQIKKEVVRFIRDFDMP

Sequences:

>Translated_417_residues
MRDDQIFNLIEKEKLREREHIELIASENFTSLEIRQAVGSILTNKYAEGYPLNRYYGGCSFIDEIETLAISRAKELFGAK
YANVQPHSGSQANMAAIMALISPGDRILGMQLSHGGHLTHGSRVNFSGIFFNTYFYGVSRDSELIDYDEVLKIAKDCRPN
LIIAGASSYSREIDFKKFREIADDVSAYLLCDIAHIAGLIVAGFHNSSIDVAHLTTSTTHKTLRGPRGGIILSGKDFDKL
VNFNGKEKPLFNAVNSTVFPGTQGGPLVHVIAGKAIAFKEALQESFKEYIANVIKNTKVMAEYFKSEGFRIVSGGTDNHL
FLVDLSSSDLTGADAEKLLESVNITLNKNAIPFDKKSPSLASGIRIGGAAITSRGLNESDSLNVAKFIVRALKAKSDIEL
KQIKKEVVRFIRDFDMP
>Mature_417_residues
MRDDQIFNLIEKEKLREREHIELIASENFTSLEIRQAVGSILTNKYAEGYPLNRYYGGCSFIDEIETLAISRAKELFGAK
YANVQPHSGSQANMAAIMALISPGDRILGMQLSHGGHLTHGSRVNFSGIFFNTYFYGVSRDSELIDYDEVLKIAKDCRPN
LIIAGASSYSREIDFKKFREIADDVSAYLLCDIAHIAGLIVAGFHNSSIDVAHLTTSTTHKTLRGPRGGIILSGKDFDKL
VNFNGKEKPLFNAVNSTVFPGTQGGPLVHVIAGKAIAFKEALQESFKEYIANVIKNTKVMAEYFKSEGFRIVSGGTDNHL
FLVDLSSSDLTGADAEKLLESVNITLNKNAIPFDKKSPSLASGIRIGGAAITSRGLNESDSLNVAKFIVRALKAKSDIEL
KQIKKEVVRFIRDFDMP

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=448, Percent_Identity=43.75, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862350, Length=448, Percent_Identity=43.75, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI261862348, Length=448, Percent_Identity=43.75, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI19923315, Length=448, Percent_Identity=43.75, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI22547186, Length=420, Percent_Identity=45.9523809523809, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=42.8571428571429, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI22547189, Length=417, Percent_Identity=41.9664268585132, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1788902, Length=407, Percent_Identity=53.8083538083538, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25144732, Length=429, Percent_Identity=43.5897435897436, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=429, Percent_Identity=43.5897435897436, Blast_Score=336, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=409, Percent_Identity=43.520782396088, Blast_Score=337, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=423, Percent_Identity=42.3167848699764, Blast_Score=329, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24640005, Length=451, Percent_Identity=44.7893569844789, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=451, Percent_Identity=44.7893569844789, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45882; Mature: 45882

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDDQIFNLIEKEKLREREHIELIASENFTSLEIRQAVGSILTNKYAEGYPLNRYYGGCS
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCH
FIDEIETLAISRAKELFGAKYANVQPHSGSQANMAAIMALISPGDRILGMQLSHGGHLTH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
GSRVNFSGIFFNTYFYGVSRDSELIDYDEVLKIAKDCRPNLIIAGASSYSREIDFKKFRE
CCEECEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
IADDVSAYLLCDIAHIAGLIVAGFHNSSIDVAHLTTSTTHKTLRGPRGGIILSGKDFDKL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHCCCCCCEEECCCCHHHH
VNFNGKEKPLFNAVNSTVFPGTQGGPLVHVIAGKAIAFKEALQESFKEYIANVIKNTKVM
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEYFKSEGFRIVSGGTDNHLFLVDLSSSDLTGADAEKLLESVNITLNKNAIPFDKKSPSL
HHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEECCCCCCCCCCCCCH
ASGIRIGGAAITSRGLNESDSLNVAKFIVRALKAKSDIELKQIKKEVVRFIRDFDMP
HHCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRDDQIFNLIEKEKLREREHIELIASENFTSLEIRQAVGSILTNKYAEGYPLNRYYGGCS
CCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCH
FIDEIETLAISRAKELFGAKYANVQPHSGSQANMAAIMALISPGDRILGMQLSHGGHLTH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCC
GSRVNFSGIFFNTYFYGVSRDSELIDYDEVLKIAKDCRPNLIIAGASSYSREIDFKKFRE
CCEECEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
IADDVSAYLLCDIAHIAGLIVAGFHNSSIDVAHLTTSTTHKTLRGPRGGIILSGKDFDKL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHCCCCCCEEECCCCHHHH
VNFNGKEKPLFNAVNSTVFPGTQGGPLVHVIAGKAIAFKEALQESFKEYIANVIKNTKVM
HCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AEYFKSEGFRIVSGGTDNHLFLVDLSSSDLTGADAEKLLESVNITLNKNAIPFDKKSPSL
HHHHHCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEECCCCCCCCCCCCCH
ASGIRIGGAAITSRGLNESDSLNVAKFIVRALKAKSDIELKQIKKEVVRFIRDFDMP
HHCCEECCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA