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The map label for this gene is ligA [H]

Identifier: 15594897

GI number: 15594897

Start: 562185

End: 564167

Strand: Direct

Name: ligA [H]

Synonym: BB0552

Alternate gene names: 15594897

Gene position: 562185-564167 (Clockwise)

Preceding gene: 15594890

Following gene: 15594899

Centisome position: 61.73

GC content: 28.85

Gene sequence:

>1983_bases
ATGAGCAGCAAAGTACAGCAAGAAATTGCAGACTTGAAGAAGTTGATTAGAAAGTGGGATAAAGAATATTATGTCGATTC
TTTGCCTAGTGTTGAAGATTTTGTATATGATAAGCATATTTTAAGGCTTCAAGAGCTAGAAAGTAAGTACCCTGAATATA
AGACCTTAGATTCTCCTACTCTTAAATTTGGCAGCGATCTTTTAAATGATTTTAAAGAGGTTGAACATTCTGCACCTATA
TTAAGTCTTGATAAGGTTTATGATCTTGATTTGCTAAAATCATGGATAGATAAGATTGATTTTAATAATTCTTTTAACAT
TTCTGTTGAGCCAAAGATTGATGGATGTTCTATCGTTCTTTATTATAAAGATGGCGTTCTTGAAAAAGCTCTTACTAGAG
GTAATGGAAAATTTGGTAATGATGTTACTATAAACGTTAGAACCATTAGATATATACCTTTATTTCTTGATGAAAAGGTT
GATTTAGTATTAAGGGGTGAGGTTTATATTACTAAAGAAAATTTTTTGAAAATAAATAAATTTTTGGAAAAGCCTTATAC
GAATTCTAGAAATTTGGCTTCGGGAATACTTAGAAGGGTTGATAGTAGAGAAGTCGCTAATTTTCCTTTAAATATTTTCA
TTTATGATTTTTTGAATGCTGGATTAGAATTTAAAACCAATGATTTAGCTACTGCAAGACTTAAGAAATTGGGTTTTAAA
GTCAATCCCTTGATTAGGTTTTTTGATCTAAAAAATTCAATTGGAGAAGTTTTAAATTACATAGCAGATATAACAAAAAA
AAGAGATTCTTTTGAATATGAAATAGATGGTGTTGTTCTTAAGGTTAGTGATTTTGCTTTAAGAGAAAGATTGGGGTATA
CTGCACATCATCCCAAATGGGCAATGGCTTACAAATTTGAAGCGCTTTCGGGTTTTAGTAGGGTAAATAGCATTGTTGTT
CAGGTTGGACGTAGTGGTAAAATTACTCCGGTTGCTAATATTGATAAAGTTTTTGTTTCAGGAGCTTTTATTACTAGTGC
AACGTTACACAATCAAGATTATATAAGGTCTATTGGGTTGAATGTTGGTGATGTTGTTAAAGTTTCAAGAAGAGGAGATG
TAATTCCTGCTGTTGAAATGGTGATAAATAAATTTTCAACAGGATTTTTCAAAGTTCCTGATAATTGCCCAGCTTGTAAA
ACGGCTGTAGTAAAAGAGGGGGCACATTTTTTTTGTCCAAATAATAATTGTCCTTCAGTAGCAGTTGAGAGAATAAAATA
TTTTTGTAGTAAAAATTGTATGGATATTGAAGGGTTTTCCGACAAGATAATTTCTTTTCTTTTTGAAAAAAAATTTATTT
TTTCAGAAATTGATCTTTATACTTTTGATTTTTATAAGCTTCTTGAATTTAAAGGGTTTAAAGATAGAAAGATAAATAAT
TTGATAAATTCAATTGAAGCTAGCAAAAAAAAACCATTTAGTAAATTACTTCTTAGTATGGGAATTAAAGATTTAGGGGA
AAATACAATAAGGTTGTTGTTTCTTAATAATTTAAATTCATTTTCAAAGCTTTTTAAGCTTTGTCAAGACAGATATTTTG
CATTTTCAACATTGTTGAAAATTAAAGGCATAGGAGAAAAAATTGCTTTAAATATTATTGAAGCTTTTAATGATTCAGTA
ATGCTTAATAAGTTTAAATTTTTTGAAAATTTGGAATTTAAAATGGAAGAGGTTGTTGCGATTGATGGTGAGAATAAGTT
ATTGGCCGGTAAAAAGTTTTGCATTACTGGAACTTTTAATGGTTATTCCAGGTCTATTATTATTGATAAGCTAAAAAATA
AAGGAGCAATTTTTAATACTTGTGTGACTGGGAGTTTAGATTTTCTTATTGTAGGAGAAAAAGCTGGATCAAAGCTTAAA
AAAGCTTTGAGTTTAAATATAAAAATTATGTCTTTTGAAGACATAAAAAGTTACTTAGATTAG

Upstream 100 bases:

>100_bases
ATGTATTTTTGGTGGTATTTTACAGTTTGTATAGTAAAGTCGGTTTTTGCTTGTTAGAATTGTTTGATAAAATAATAAAG
AATATGTTAAATTTTACCCT

Downstream 100 bases:

>100_bases
TTGGATAAAAGTGTTTATTTTTATTTAATTATTTTTATTTGCTTAAATTCAGGCTTTAGATTTTTATACATAATTCCTAG
TTCGTGGATATTTTTGACTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSSKVQQEIADLKKLIRKWDKEYYVDSLPSVEDFVYDKHILRLQELESKYPEYKTLDSPTLKFGSDLLNDFKEVEHSAPI
LSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGVLEKALTRGNGKFGNDVTINVRTIRYIPLFLDEKV
DLVLRGEVYITKENFLKINKFLEKPYTNSRNLASGILRRVDSREVANFPLNIFIYDFLNAGLEFKTNDLATARLKKLGFK
VNPLIRFFDLKNSIGEVLNYIADITKKRDSFEYEIDGVVLKVSDFALRERLGYTAHHPKWAMAYKFEALSGFSRVNSIVV
QVGRSGKITPVANIDKVFVSGAFITSATLHNQDYIRSIGLNVGDVVKVSRRGDVIPAVEMVINKFSTGFFKVPDNCPACK
TAVVKEGAHFFCPNNNCPSVAVERIKYFCSKNCMDIEGFSDKIISFLFEKKFIFSEIDLYTFDFYKLLEFKGFKDRKINN
LINSIEASKKKPFSKLLLSMGIKDLGENTIRLLFLNNLNSFSKLFKLCQDRYFAFSTLLKIKGIGEKIALNIIEAFNDSV
MLNKFKFFENLEFKMEEVVAIDGENKLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFNTCVTGSLDFLIVGEKAGSKLK
KALSLNIKIMSFEDIKSYLD

Sequences:

>Translated_660_residues
MSSKVQQEIADLKKLIRKWDKEYYVDSLPSVEDFVYDKHILRLQELESKYPEYKTLDSPTLKFGSDLLNDFKEVEHSAPI
LSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGVLEKALTRGNGKFGNDVTINVRTIRYIPLFLDEKV
DLVLRGEVYITKENFLKINKFLEKPYTNSRNLASGILRRVDSREVANFPLNIFIYDFLNAGLEFKTNDLATARLKKLGFK
VNPLIRFFDLKNSIGEVLNYIADITKKRDSFEYEIDGVVLKVSDFALRERLGYTAHHPKWAMAYKFEALSGFSRVNSIVV
QVGRSGKITPVANIDKVFVSGAFITSATLHNQDYIRSIGLNVGDVVKVSRRGDVIPAVEMVINKFSTGFFKVPDNCPACK
TAVVKEGAHFFCPNNNCPSVAVERIKYFCSKNCMDIEGFSDKIISFLFEKKFIFSEIDLYTFDFYKLLEFKGFKDRKINN
LINSIEASKKKPFSKLLLSMGIKDLGENTIRLLFLNNLNSFSKLFKLCQDRYFAFSTLLKIKGIGEKIALNIIEAFNDSV
MLNKFKFFENLEFKMEEVVAIDGENKLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFNTCVTGSLDFLIVGEKAGSKLK
KALSLNIKIMSFEDIKSYLD
>Mature_659_residues
SSKVQQEIADLKKLIRKWDKEYYVDSLPSVEDFVYDKHILRLQELESKYPEYKTLDSPTLKFGSDLLNDFKEVEHSAPIL
SLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVLYYKDGVLEKALTRGNGKFGNDVTINVRTIRYIPLFLDEKVD
LVLRGEVYITKENFLKINKFLEKPYTNSRNLASGILRRVDSREVANFPLNIFIYDFLNAGLEFKTNDLATARLKKLGFKV
NPLIRFFDLKNSIGEVLNYIADITKKRDSFEYEIDGVVLKVSDFALRERLGYTAHHPKWAMAYKFEALSGFSRVNSIVVQ
VGRSGKITPVANIDKVFVSGAFITSATLHNQDYIRSIGLNVGDVVKVSRRGDVIPAVEMVINKFSTGFFKVPDNCPACKT
AVVKEGAHFFCPNNNCPSVAVERIKYFCSKNCMDIEGFSDKIISFLFEKKFIFSEIDLYTFDFYKLLEFKGFKDRKINNL
INSIEASKKKPFSKLLLSMGIKDLGENTIRLLFLNNLNSFSKLFKLCQDRYFAFSTLLKIKGIGEKIALNIIEAFNDSVM
LNKFKFFENLEFKMEEVVAIDGENKLLAGKKFCITGTFNGYSRSIIIDKLKNKGAIFNTCVTGSLDFLIVGEKAGSKLKK
ALSLNIKIMSFEDIKSYLD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=33.7817638266069, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI87082305, Length=569, Percent_Identity=23.0228471001757, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75317; Mature: 75186

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50172 BRCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKVQQEIADLKKLIRKWDKEYYVDSLPSVEDFVYDKHILRLQELESKYPEYKTLDSPT
CCHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKFGSDLLNDFKEVEHSAPILSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
YYKDGVLEKALTRGNGKFGNDVTINVRTIRYIPLFLDEKVDLVLRGEVYITKENFLKINK
EECCCHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEECCCCCEEEECEEEEEHHHHHHHHH
FLEKPYTNSRNLASGILRRVDSREVANFPLNIFIYDFLNAGLEFKTNDLATARLKKLGFK
HHCCCCCCCCHHHHHHHHHCCCCHHHCCCCEEEEEEHHCCCCEECCCCHHHHHHHHCCCC
VNPLIRFFDLKNSIGEVLNYIADITKKRDSFEYEIDGVVLKVSDFALRERLGYTAHHPKW
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEEEHHHHHHHHCCCCCCCCCE
AMAYKFEALSGFSRVNSIVVQVGRSGKITPVANIDKVFVSGAFITSATLHNQDYIRSIGL
EEEEEEHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHCCCEEEEEEECCCHHHHHHHCC
NVGDVVKVSRRGDVIPAVEMVINKFSTGFFKVPDNCPACKTAVVKEGAHFFCPNNNCPSV
CHHHHEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCEEECCCCCCHHH
AVERIKYFCSKNCMDIEGFSDKIISFLFEKKFIFSEIDLYTFDFYKLLEFKGFKDRKINN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHH
LINSIEASKKKPFSKLLLSMGIKDLGENTIRLLFLNNLNSFSKLFKLCQDRYFAFSTLLK
HHHHHHHHCCCCHHHHHHHHCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IKGIGEKIALNIIEAFNDSVMLNKFKFFENLEFKMEEVVAIDGENKLLAGKKFCITGTFN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCEEEEEECCC
GYSRSIIIDKLKNKGAIFNTCVTGSLDFLIVGEKAGSKLKKALSLNIKIMSFEDIKSYLD
CCCCEEEEEHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHEEEEEEEEHHHHHHHCC
>Mature Secondary Structure 
SSKVQQEIADLKKLIRKWDKEYYVDSLPSVEDFVYDKHILRLQELESKYPEYKTLDSPT
CHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKFGSDLLNDFKEVEHSAPILSLDKVYDLDLLKSWIDKIDFNNSFNISVEPKIDGCSIVL
HHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
YYKDGVLEKALTRGNGKFGNDVTINVRTIRYIPLFLDEKVDLVLRGEVYITKENFLKINK
EECCCHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEECCCCCEEEECEEEEEHHHHHHHHH
FLEKPYTNSRNLASGILRRVDSREVANFPLNIFIYDFLNAGLEFKTNDLATARLKKLGFK
HHCCCCCCCCHHHHHHHHHCCCCHHHCCCCEEEEEEHHCCCCEECCCCHHHHHHHHCCCC
VNPLIRFFDLKNSIGEVLNYIADITKKRDSFEYEIDGVVLKVSDFALRERLGYTAHHPKW
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEEEHHHHHHHHCCCCCCCCCE
AMAYKFEALSGFSRVNSIVVQVGRSGKITPVANIDKVFVSGAFITSATLHNQDYIRSIGL
EEEEEEHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHCCCEEEEEEECCCHHHHHHHCC
NVGDVVKVSRRGDVIPAVEMVINKFSTGFFKVPDNCPACKTAVVKEGAHFFCPNNNCPSV
CHHHHEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCEEECCCCCCHHH
AVERIKYFCSKNCMDIEGFSDKIISFLFEKKFIFSEIDLYTFDFYKLLEFKGFKDRKINN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHH
LINSIEASKKKPFSKLLLSMGIKDLGENTIRLLFLNNLNSFSKLFKLCQDRYFAFSTLLK
HHHHHHHHCCCCHHHHHHHHCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
IKGIGEKIALNIIEAFNDSVMLNKFKFFENLEFKMEEVVAIDGENKLLAGKKFCITGTFN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCEEEEEECCC
GYSRSIIIDKLKNKGAIFNTCVTGSLDFLIVGEKAGSKLKKALSLNIKIMSFEDIKSYLD
CCCCEEEEEHHCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHEEEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA