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The map label for this gene is yfgF [H]

Identifier: 15594708

GI number: 15594708

Start: 371034

End: 373046

Strand: Direct

Name: yfgF [H]

Synonym: BB0363

Alternate gene names: 15594708

Gene position: 371034-373046 (Clockwise)

Preceding gene: 15594707

Following gene: 15594709

Centisome position: 40.74

GC content: 24.29

Gene sequence:

>2013_bases
ATGAACAAAGTCAATAATTATCAAAATATAAATTCAGTTATAATCTCTAAAAAAGAAGTTGGACTTAGAGATCTCATAAA
GCTTAAATCTATTTTTAACTTAATTCAAATCGTAAAATCTGAAAAAGCTTTATACAGCGAATATGTAAAACAAAATAATA
TTAAATTCGCTATTATTTATAATTATGAAAAACCAATAGATTTTTCAATAAACATTGCAAACGAATTAAAAAATGCAAAC
AAAATCCATTCTATCATAATTAGCAAAGAAAAATTTGATGAGGAATATTTAAAACTTGATCACATAGAAATAATAAAAGA
TATAAGCGAATTAGAATATAAGCAAAGCTTAATACATCAAAAAAAATTATTTTGCGACAATAAAAACACAACTCTGGATT
TCTTTTTAAATTTATCAGAACTCATAAAAGAAATAGTAATCATCACAAACACTAAAAATGAAATTATTTATATCAATGAA
AAAGGTAGTAAAAATCTTAATCTTCCAATGAAAACCTCTGGAAATATAATCAAAGTAACCGACATAGATATCAGAGATTG
GGAAAAGCTAGAAAAAATAAATTTAAGTTATCACACAAATTCTATTCCTGAATTTAAAAATATATTAATAACTGATTGTC
TTTTAACTTTAAAAAATAATAAAAAACTACATGTGGATATATTTATTAGCACAATTGCTCAAAACAATATCGATAAATTA
ATAACAATTAAAGAAATATCAAACTCTAATAAAATAGAAAACTATAAATATCTAGAAATTATTGATTCGCAAGACGAGAT
TCAAAATGCCAAAGAAATTGAAAAATTACTAGTAAACCATATGGATATTTACAAAAAAAAAAGCATATATTTACTTAATT
TAGACGTATCCCTAACAGCAGAATATGAATACAAAGAAGATCAAGAAAAACTTAATGCAAAAATACTTAAAATAATGTAT
TCAAAAATAATGTCATTATACTCTGAATATATTTTTAAGTTAAAACACAACAATTTAATAGTAATCATAAGCACAAGTGG
CGGAGAAAAGCGAATAATCTCAATTGCCAAAAAAATCAAAAAAACAATTGCTCTGGCATTTAAAAAAGAAGATATTATTA
TATTCAAGTTCAACATTGGAATAATAGAGGTAAATTTAAAAGAAAACTTAGAATTCAAAATCCCCAAATTAATGATGGCT
ACAAAAATATCATCGGAATACAAAGAATCTAATCCCACTATATACAAAGAAGAACTACCAGAAGCGGTGATTTTAAAAAA
TCAAAACAAAATTTTTCAATATATACTCAAAGCAATAAAAAATGATTTTTTTACTCTTTATTATCAAAAAATAAATCCTC
TTAAAAAGAACTTAAAGCCCAAAATAGAAATCTTGACAAGACTTTTTGACCACATGGGCAAACCAATTCCAAACAATCAA
ATTTTTAACTTAATAGACAAATATAATTTAACTGTTGAGGTTGATACATTGGTGGTTAAAAAGGCTTTAAGAGAATACAA
AAGCTTTGTATCAAAAAATGGAATTCACATTTTCTCAATTAACATATCTCCTTATTCACTAAAATCCCAAAACTTTCGAA
TCTTTTTAAGAGATACTTTATTGAAAAGCCAAATCCCACTTCAAAATATATGCTTGGAAATAACAGAAACTGGAATTCTT
GAAAACTTTGAGATAATAAACAAATATTTTCAAGAATTAAAAAGTTTTGGAATCAAGCTAGCACTTGATGACTTTGGAAG
CGGACATACATCACTCTCATATATTAAAACACTACCAATAGACTTACTTAAAATAGACGGATCTTTCATAAAAGCAATAA
ACTCTAGTGAAATAGATTTTGTAATAATAAAATCTATTAAAAAAATAGCAGATACAAAAAATATAAAAATTATTGCCGAA
TTTGTGTATAATGAGGAAATATTAAAAAAAATAATTGAACTAGAAATAGACTATGGGCAAGGATTTTTATGGCACAAACC
AGAACCAATATAA

Upstream 100 bases:

>100_bases
GATTAATCTGGATAATAGTCACTAAAAAAATTGCAGATAAAAAAATAAAAAATAACACCAACTTGGCATACAAAAATTAA
AATAAGAGCAAGCCAATAAA

Downstream 100 bases:

>100_bases
ATTCTAAAAAAGTGAAATCTAAAAATATACTTATGCCTAAATTTTATTAAATTTCAACAAAAATTATTTTACATTGAAAA
AGGAATAAAATTAATGGAAA

Product: periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MNKVNNYQNINSVIISKKEVGLRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNYEKPIDFSINIANELKNAN
KIHSIIISKEKFDEEYLKLDHIEIIKDISELEYKQSLIHQKKLFCDNKNTTLDFFLNLSELIKEIVIITNTKNEIIYINE
KGSKNLNLPMKTSGNIIKVTDIDIRDWEKLEKINLSYHTNSIPEFKNILITDCLLTLKNNKKLHVDIFISTIAQNNIDKL
ITIKEISNSNKIENYKYLEIIDSQDEIQNAKEIEKLLVNHMDIYKKKSIYLLNLDVSLTAEYEYKEDQEKLNAKILKIMY
SKIMSLYSEYIFKLKHNNLIVIISTSGGEKRIISIAKKIKKTIALAFKKEDIIIFKFNIGIIEVNLKENLEFKIPKLMMA
TKISSEYKESNPTIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNNQ
IFNLIDKYNLTVEVDTLVVKKALREYKSFVSKNGIHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDFVIIKSIKKIADTKNIKIIAE
FVYNEEILKKIIELEIDYGQGFLWHKPEPI

Sequences:

>Translated_670_residues
MNKVNNYQNINSVIISKKEVGLRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNYEKPIDFSINIANELKNAN
KIHSIIISKEKFDEEYLKLDHIEIIKDISELEYKQSLIHQKKLFCDNKNTTLDFFLNLSELIKEIVIITNTKNEIIYINE
KGSKNLNLPMKTSGNIIKVTDIDIRDWEKLEKINLSYHTNSIPEFKNILITDCLLTLKNNKKLHVDIFISTIAQNNIDKL
ITIKEISNSNKIENYKYLEIIDSQDEIQNAKEIEKLLVNHMDIYKKKSIYLLNLDVSLTAEYEYKEDQEKLNAKILKIMY
SKIMSLYSEYIFKLKHNNLIVIISTSGGEKRIISIAKKIKKTIALAFKKEDIIIFKFNIGIIEVNLKENLEFKIPKLMMA
TKISSEYKESNPTIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNNQ
IFNLIDKYNLTVEVDTLVVKKALREYKSFVSKNGIHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDFVIIKSIKKIADTKNIKIIAE
FVYNEEILKKIIELEIDYGQGFLWHKPEPI
>Mature_670_residues
MNKVNNYQNINSVIISKKEVGLRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNYEKPIDFSINIANELKNAN
KIHSIIISKEKFDEEYLKLDHIEIIKDISELEYKQSLIHQKKLFCDNKNTTLDFFLNLSELIKEIVIITNTKNEIIYINE
KGSKNLNLPMKTSGNIIKVTDIDIRDWEKLEKINLSYHTNSIPEFKNILITDCLLTLKNNKKLHVDIFISTIAQNNIDKL
ITIKEISNSNKIENYKYLEIIDSQDEIQNAKEIEKLLVNHMDIYKKKSIYLLNLDVSLTAEYEYKEDQEKLNAKILKIMY
SKIMSLYSEYIFKLKHNNLIVIISTSGGEKRIISIAKKIKKTIALAFKKEDIIIFKFNIGIIEVNLKENLEFKIPKLMMA
TKISSEYKESNPTIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNNQ
IFNLIDKYNLTVEVDTLVVKKALREYKSFVSKNGIHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDFVIIKSIKKIADTKNIKIIAE
FVYNEEILKKIIELEIDYGQGFLWHKPEPI

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=373, Percent_Identity=26.8096514745308, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI87082096, Length=209, Percent_Identity=31.1004784688995, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788502, Length=240, Percent_Identity=28.75, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787541, Length=247, Percent_Identity=27.1255060728745, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI226510982, Length=239, Percent_Identity=26.7782426778243, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081921, Length=235, Percent_Identity=26.8085106382979, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081980, Length=234, Percent_Identity=29.0598290598291, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87081743, Length=249, Percent_Identity=26.9076305220884, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081845, Length=236, Percent_Identity=29.2372881355932, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787055, Length=184, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790496, Length=233, Percent_Identity=24.4635193133047, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=27.3109243697479, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789650, Length=305, Percent_Identity=20, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 78211; Mature: 78211

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVNNYQNINSVIISKKEVGLRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIY
CCCCCCCCCCHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NYEKPIDFSINIANELKNANKIHSIIISKEKFDEEYLKLDHIEIIKDISELEYKQSLIHQ
ECCCCCEEEEEEHHHHCCHHHHHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLFCDNKNTTLDFFLNLSELIKEIVIITNTKNEIIYINEKGSKNLNLPMKTSGNIIKVT
HHHHCCCCCCEEEEEECHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
DIDIRDWEKLEKINLSYHTNSIPEFKNILITDCLLTLKNNKKLHVDIFISTIAQNNIDKL
ECCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHEECCCCEEEEEEEEEEHHCCCCCCE
ITIKEISNSNKIENYKYLEIIDSQDEIQNAKEIEKLLVNHMDIYKKKSIYLLNLDVSLTA
EEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
EYEYKEDQEKLNAKILKIMYSKIMSLYSEYIFKLKHNNLIVIISTSGGEKRIISIAKKIK
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHHHHHH
KTIALAFKKEDIIIFKFNIGIIEVNLKENLEFKIPKLMMATKISSEYKESNPTIYKEELP
HHHHHEEECCCEEEEEEEEEEEEEEECCCCCEECCHHHHHHHHHHHHHCCCCCEEHHHCC
EAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNNQ
CEEEEECCHHHHHHHHHHHCCCCEEEEHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCH
IFNLIDKYNLTVEVDTLVVKKALREYKSFVSKNGIHIFSINISPYSLKSQNFRIFLRDTL
HHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEEHH
LKSQIPLQNICLEITETGILENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCE
DLLKIDGSFIKAINSSEIDFVIIKSIKKIADTKNIKIIAEFVYNEEILKKIIELEIDYGQ
EEEEECCHHHHHCCCCCCCEEEHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHEEEECCC
GFLWHKPEPI
CEECCCCCCC
>Mature Secondary Structure
MNKVNNYQNINSVIISKKEVGLRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIY
CCCCCCCCCCHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NYEKPIDFSINIANELKNANKIHSIIISKEKFDEEYLKLDHIEIIKDISELEYKQSLIHQ
ECCCCCEEEEEEHHHHCCHHHHHHHEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLFCDNKNTTLDFFLNLSELIKEIVIITNTKNEIIYINEKGSKNLNLPMKTSGNIIKVT
HHHHCCCCCCEEEEEECHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEE
DIDIRDWEKLEKINLSYHTNSIPEFKNILITDCLLTLKNNKKLHVDIFISTIAQNNIDKL
ECCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHEECCCCEEEEEEEEEEHHCCCCCCE
ITIKEISNSNKIENYKYLEIIDSQDEIQNAKEIEKLLVNHMDIYKKKSIYLLNLDVSLTA
EEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
EYEYKEDQEKLNAKILKIMYSKIMSLYSEYIFKLKHNNLIVIISTSGGEKRIISIAKKIK
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCHHHHHHHHHHH
KTIALAFKKEDIIIFKFNIGIIEVNLKENLEFKIPKLMMATKISSEYKESNPTIYKEELP
HHHHHEEECCCEEEEEEEEEEEEEEECCCCCEECCHHHHHHHHHHHHHCCCCCEEHHHCC
EAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNNQ
CEEEEECCHHHHHHHHHHHCCCCEEEEHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCH
IFNLIDKYNLTVEVDTLVVKKALREYKSFVSKNGIHIFSINISPYSLKSQNFRIFLRDTL
HHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEEHH
LKSQIPLQNICLEITETGILENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCE
DLLKIDGSFIKAINSSEIDFVIIKSIKKIADTKNIKIIAEFVYNEEILKKIIELEIDYGQ
EEEEECCHHHHHCCCCCCCEEEHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHEEEECCC
GFLWHKPEPI
CEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]