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The map label for this gene is pyk

Identifier: 15594693

GI number: 15594693

Start: 357007

End: 358440

Strand: Direct

Name: pyk

Synonym: BB0348

Alternate gene names: 15594693

Gene position: 357007-358440 (Clockwise)

Preceding gene: 15594692

Following gene: 15594694

Centisome position: 39.2

GC content: 32.29

Gene sequence:

>1434_bases
ATGATTTCAAAGTTAACAAAAATTGTAGCAACAATATCTGATCTTAGATGCGAACCAGAACATATAAAAGATTTACACGA
TGCAGGGGTAAATGTTATAAGACTAAATACTGCTCATCAATCACACGAAGATACAATAAAAGTAATAGACAATGTTAGAA
AAATTTCAAATAAAATAGCTTTAATGATTGATACAAAAGGACCAGAAGTTAGAACAGCAAATATTGAAAATCCTATTATT
GTAAAAACTGGAGACAAGGTAATCATTTCAACCTCGCCTATTAATGAGCCTAATAACTTTCAAACCAATTATGATGGATT
TGTTAAAGAAGTACCCCAAGGATCTAAAGTGCTAATTGATGACGGTGAACTTGAAATGACTGTTGTTGCCAAATTGCCTG
ACAGATTAATTTGTGAAATTAAAAATGACGGCCAAATTAAAAATAAAAAATCAATCAATACCCCCGGAATTTCTCTTAAG
CTACAATCAGTAACCGAAAAAGACAAAGGATTTATTGAGCTTGCAGCAAAATATAATGTTGATTTTATTGCCCATTCGTT
TGTAAGACATTCCAAAGACGTTCAAGATGTTCAAGAAATTTTAACTGCCTCTGGAAATCCTGATGTAAAAATTATATCCA
AAATCGAAAATCAAGAGGGAATTGACAACATTGAAGAAATTGCAAAAGCTTCTTACGGAATAATGGTTGCAAGGGGAGAC
ATGGGAGTTGAAATTCCTGCAGAAGATGTACCCATTGCTCAACTTAAAATAACACAAACCTGTATAAAGTATGGAATACC
CGTGATTACAGCAACTCAAATGCTTCATACAATGATTGAAAATCCAAGACCTACTAGAGCAGAAGTGTCTGACATCGCTA
ACGCTATTTTAAACGGCACAGACGCAATTATGTTATCCGGAGAAACCGCCTACGGGAAATATCCAATTGAAGCTGTAAAA
ATGATGACAAGCATTGCTAAAAAAGTTGAAAAACACAGAAAAATGACCTTATATAAGGATGAACTTTTTTACGATAAAAG
TATCACAAGAAACTATATTATCAAATGTGCAATTGATGCCACAAAACTTATGGACATAAAAGCAATTATTGTAGATTCTC
TAAAAGGCAAAACCGCAAGAATAATGGCAACCTACAGAGCAAGTGTTCCATTATTCATTACAACAAATAGCGAAAGACTA
GCAAGAGAATTAGCATTATCTTACGGGGTTTATTCTAATCTTGTAGACAATAATTTCAAAAGAACTACCGAATTTGTAGT
AACTTCTCTTAAAATGTTAAAAGAACAAGGTGTTGTTAATGACAAAGATACTGTAATAATTATTTCTGGAAATCCAAATA
GAAATATTGAAAAAGGAACAGAATTTATGGAAATAAACACAGTAGAAGATGCAATCAAAGGACGAAATATATAA

Upstream 100 bases:

>100_bases
TAAACACTGCTTATAGCAAAGTAAAGTTTTGAATTTTTTACAAAATATTGTTATTCAATGACAAAAAGCATAAAATTTAT
AAAAGTGGAGGAAGAACTTT

Downstream 100 bases:

>100_bases
AGCAAGCTAGAAACTCCTTGGTTGAAAACATATTTTATTCAGATAATACAAACGACTTGTCATTTAAGTTGGAAGAAAAA
AAAACTCACAAAGCCCTCCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MISKLTKIVATISDLRCEPEHIKDLHDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDKVIISTSPINEPNNFQTNYDGFVKEVPQGSKVLIDDGELEMTVVAKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRHSKDVQDVQEILTASGNPDVKIISKIENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDIKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELALSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQGVVNDKDTVIIISGNPNRNIEKGTEFMEINTVEDAIKGRNI

Sequences:

>Translated_477_residues
MISKLTKIVATISDLRCEPEHIKDLHDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDKVIISTSPINEPNNFQTNYDGFVKEVPQGSKVLIDDGELEMTVVAKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRHSKDVQDVQEILTASGNPDVKIISKIENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDIKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELALSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQGVVNDKDTVIIISGNPNRNIEKGTEFMEINTVEDAIKGRNI
>Mature_477_residues
MISKLTKIVATISDLRCEPEHIKDLHDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDKVIISTSPINEPNNFQTNYDGFVKEVPQGSKVLIDDGELEMTVVAKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRHSKDVQDVQEILTASGNPDVKIISKIENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDIKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELALSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQGVVNDKDTVIIISGNPNRNIEKGTEFMEINTVEDAIKGRNI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI10835121, Length=480, Percent_Identity=36.25, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI32967597, Length=480, Percent_Identity=36.25, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI33286422, Length=474, Percent_Identity=37.1308016877637, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI33286420, Length=474, Percent_Identity=37.1308016877637, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI33286418, Length=475, Percent_Identity=37.0526315789474, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=37.4125874125874, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=37.4125874125874, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI310128736, Length=234, Percent_Identity=33.7606837606838, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128734, Length=234, Percent_Identity=33.7606837606838, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI310128738, Length=212, Percent_Identity=34.4339622641509, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1787965, Length=457, Percent_Identity=40.0437636761488, Blast_Score=321, Evalue=8e-89,
Organism=Escherichia coli, GI1788160, Length=463, Percent_Identity=32.6133909287257, Blast_Score=218, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17544584, Length=468, Percent_Identity=35.4700854700855, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=475, Percent_Identity=33.8947368421053, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17506829, Length=475, Percent_Identity=33.8947368421053, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI71984406, Length=475, Percent_Identity=33.8947368421053, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=475, Percent_Identity=33.8947368421053, Blast_Score=247, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=36.0995850622407, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=470, Percent_Identity=36.1702127659575, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24648966, Length=458, Percent_Identity=37.3362445414847, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24648964, Length=477, Percent_Identity=35.6394129979036, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI28571814, Length=477, Percent_Identity=35.6394129979036, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24581235, Length=504, Percent_Identity=30.952380952381, Blast_Score=231, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24646914, Length=279, Percent_Identity=40.1433691756272, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_BORBU (O51323)

Other databases:

- EMBL:   AE000783
- PIR:   C70143
- RefSeq:   NP_212482.1
- ProteinModelPortal:   O51323
- SMR:   O51323
- EnsemblBacteria:   EBBORT00000009033
- GeneID:   1195185
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0348
- NMPDR:   fig|224326.1.peg.732
- TIGR:   BB_0348
- GeneTree:   EBGT00050000006920
- HOGENOM:   HBG734376
- OMA:   IVYTESG
- PhylomeDB:   O51323
- ProtClustDB:   PRK05826
- BioCyc:   BBUR224326:BB_0348-MONOMER
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 53033; Mature: 53033

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 34-34 BINDING 239-239 BINDING 240-240 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKLTKIVATISDLRCEPEHIKDLHDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPIIVKTGDKVIISTSPINEPNNFQTNYDGFVKEVPQGSKVLID
EEEECCCCCEEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEE
DGELEMTVVAKLPDRLICEIKNDGQIKNKKSINTPGISLKLQSVTEKDKGFIELAAKYNV
CCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRHSKDVQDVQEILTASGNPDVKIISKIENQEGIDNIEEIAKASYGIMVARGD
HHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECC
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGT
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPIEAVKMMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCEEEEEEECH
TKLMDIKAIIVDSLKGKTARIMATYRASVPLFITTNSERLARELALSYGVYSNLVDNNFK
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
RTTEFVVTSLKMLKEQGVVNDKDTVIIISGNPNRNIEKGTEFMEINTVEDAIKGRNI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHCCCCC
>Mature Secondary Structure
MISKLTKIVATISDLRCEPEHIKDLHDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPIIVKTGDKVIISTSPINEPNNFQTNYDGFVKEVPQGSKVLID
EEEECCCCCEEEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEE
DGELEMTVVAKLPDRLICEIKNDGQIKNKKSINTPGISLKLQSVTEKDKGFIELAAKYNV
CCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRHSKDVQDVQEILTASGNPDVKIISKIENQEGIDNIEEIAKASYGIMVARGD
HHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECC
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGT
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPIEAVKMMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCEEEEEEECH
TKLMDIKAIIVDSLKGKTARIMATYRASVPLFITTNSERLARELALSYGVYSNLVDNNFK
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
RTTEFVVTSLKMLKEQGVVNDKDTVIIISGNPNRNIEKGTEFMEINTVEDAIKGRNI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685