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The map label for this gene is rlmE

Identifier: 15594658

GI number: 15594658

Start: 319680

End: 320258

Strand: Reverse

Name: rlmE

Synonym: BB0313

Alternate gene names: 15594658

Gene position: 320258-319680 (Counterclockwise)

Preceding gene: 15594661

Following gene: 15594657

Centisome position: 35.17

GC content: 27.98

Gene sequence:

>579_bases
GTGTATCGCTTGGATGATGAATATTCTAAAAAAGCCAAAAGAGAAGGATATTTGGCAAGGTCTGTATATAAGTTGATAGA
AATTAATGAAAAATTTTCTTTATTTTCTTCTGGCAATGTTTTAGATATTGGCGCATCACCTGGCAGCTTTTCTCAGTATG
CTTATAAAAAGCTTAAAAGAGGAATTCTAGTATCTGTTGATATTAATGATATTGGCCTTAGATATGATGATAATTTTTAT
TTTATAAAGGGAGATATCTTTTTAGATGATACAGTTTTTAAAATTAATACGTTTAAACCTTATAGTCTTGTAATTAGTGA
TGTGGCTCCCAAGACTACTGGAAATAGACTTGTAGATACCAGCAATTCTTTTAATTTAAGCATGAGAATAATAGATTTAT
CACTTGAAGTTTTACTTAAAAAAGGGAATTTACTTGTTAAAGTTTTTCAGGGAGGAGACGAGATGCAAATTTTTAAAAAG
TTTGAAAAATATTTTAAATTTGTAAAAAAAATTAGACCCAAAGCTGTAAGGAAAAATTCTTTTGAAATTTATTTTTTAGG
CAAAAGTTTTGGCAAGTAG

Upstream 100 bases:

>100_bases
ATTTTATTAATATTTTTTTCAATATTTTTTAAAAATAGTTTATTTTGCATAATAAGAATTATAATGAAAAAGTATAATAA
AGTGTATTAAAGGGATTGAT

Downstream 100 bases:

>100_bases
CAAATTAATCAAATTGTTATAAACAGATTTAAAGGTATAAAATATGTTTAGAAAAGAAAGTTCTAAAGACAGCAGATCAC
AGCTTCAAGTTGCAGGTTTT

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MYRLDDEYSKKAKREGYLARSVYKLIEINEKFSLFSSGNVLDIGASPGSFSQYAYKKLKRGILVSVDINDIGLRYDDNFY
FIKGDIFLDDTVFKINTFKPYSLVISDVAPKTTGNRLVDTSNSFNLSMRIIDLSLEVLLKKGNLLVKVFQGGDEMQIFKK
FEKYFKFVKKIRPKAVRKNSFEIYFLGKSFGK

Sequences:

>Translated_192_residues
MYRLDDEYSKKAKREGYLARSVYKLIEINEKFSLFSSGNVLDIGASPGSFSQYAYKKLKRGILVSVDINDIGLRYDDNFY
FIKGDIFLDDTVFKINTFKPYSLVISDVAPKTTGNRLVDTSNSFNLSMRIIDLSLEVLLKKGNLLVKVFQGGDEMQIFKK
FEKYFKFVKKIRPKAVRKNSFEIYFLGKSFGK
>Mature_192_residues
MYRLDDEYSKKAKREGYLARSVYKLIEINEKFSLFSSGNVLDIGASPGSFSQYAYKKLKRGILVSVDINDIGLRYDDNFY
FIKGDIFLDDTVFKINTFKPYSLVISDVAPKTTGNRLVDTSNSFNLSMRIIDLSLEVLLKKGNLLVKVFQGGDEMQIFKK
FEKYFKFVKKIRPKAVRKNSFEIYFLGKSFGK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=200, Percent_Identity=30, Blast_Score=103, Evalue=8e-23,
Organism=Homo sapiens, GI7110661, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI29029591, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI29029589, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=26.7015706806283, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789569, Length=191, Percent_Identity=30.8900523560209, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17553474, Length=203, Percent_Identity=32.512315270936, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987550, Length=192, Percent_Identity=30.7291666666667, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554650, Length=189, Percent_Identity=29.1005291005291, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71987561, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17553860, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319796, Length=186, Percent_Identity=31.1827956989247, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319535, Length=196, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356387, Length=208, Percent_Identity=26.4423076923077, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648639, Length=192, Percent_Identity=26.0416666666667, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_BORBU (O51293)

Other databases:

- EMBL:   AE000783
- PIR:   H70138
- RefSeq:   NP_212447.1
- ProteinModelPortal:   O51293
- SMR:   O51293
- EnsemblBacteria:   EBBORT00000009008
- GeneID:   1195150
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0313
- NMPDR:   fig|224326.1.peg.697
- TIGR:   BB_0313
- GeneTree:   EBGT00050000007024
- HOGENOM:   HBG398270
- OMA:   AWLQVAC
- ProtClustDB:   CLSK507509
- BioCyc:   BBUR224326:BB_0313-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 22167; Mature: 22167

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: ACT_SITE 147-147 BINDING 48-48 BINDING 50-50 BINDING 67-67 BINDING 85-85 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLDDEYSKKAKREGYLARSVYKLIEINEKFSLFSSGNVLDIGASPGSFSQYAYKKLKR
CCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHC
GILVSVDINDIGLRYDDNFYFIKGDIFLDDTVFKINTFKPYSLVISDVAPKTTGNRLVDT
CEEEEEECCCEEEEECCCEEEEECCEEECCEEEEEECCCCCEEEEECCCCCCCCCEEEEC
SNSFNLSMRIIDLSLEVLLKKGNLLVKVFQGGDEMQIFKKFEKYFKFVKKIRPKAVRKNS
CCCCEEEEEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
FEIYFLGKSFGK
EEEEEECCCCCC
>Mature Secondary Structure
MYRLDDEYSKKAKREGYLARSVYKLIEINEKFSLFSSGNVLDIGASPGSFSQYAYKKLKR
CCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHC
GILVSVDINDIGLRYDDNFYFIKGDIFLDDTVFKINTFKPYSLVISDVAPKTTGNRLVDT
CEEEEEECCCEEEEECCCEEEEECCEEECCEEEEEECCCCCEEEEECCCCCCCCCEEEEC
SNSFNLSMRIIDLSLEVLLKKGNLLVKVFQGGDEMQIFKKFEKYFKFVKKIRPKAVRKNS
CCCCEEEEEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCC
FEIYFLGKSFGK
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685