Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 15594656

GI number: 15594656

Start: 318277

End: 319158

Strand: Reverse

Name: ppnK [H]

Synonym: BB0311

Alternate gene names: 15594656

Gene position: 319158-318277 (Counterclockwise)

Preceding gene: 15594657

Following gene: 15594655

Centisome position: 35.04

GC content: 28.38

Gene sequence:

>882_bases
TTGATAAATGCGAATTTGACGACATTCCCTATAAAGATCAATATGAAGAATAAAGTTCTTCTTTGCATTAATACTTTAAA
GTCGGGAGCTAGTATTTTAGGCAATGATGTTAAAGTTTATTTAGAAACCAAGTATTTTGTTGAAGTAGTGTTAATAGATG
TTGGCAGACCTTTATTTTCTTTTCCAAAAGAAAATTTTCTTTTTTTGATAACTTTAGGAGGAGATGGCACAGTTCTTTTG
GCTGTTAATTTGCTTCTTGAAAATGAAAACATTGATATTCCAATTATTTCAATTAATATGGGCAATGTGGGATTTTTAGC
AGATATTAAGATTGAAGATTTTAAAAAAGTCATAGATAGATTTTTTAACAATTCTTTGGTTATTAATAAAAAATTTTTGC
TTCATGTAACAGTTTCTCAACACGGTAAAGATTTAATTTCTAAATATGCTTTAAACGATATTATTATTCGCTCAAGCGTT
CTTAATAAAATGATTTATGTAGATCTTATGGTTAATTCTGAGAGTTTTTTATCATACAAAAGTGATGGGATAATTGTGTC
TACTCCAACAGGCTCAACAGGATATTCTTTCTCAGCAGGGGGTCCTATTTTAGAAGCAGATCTTGAGGGATTTTYACTTA
CGCCTATTTCTCCACATTCTGTTTATAATCGTTCTTTTGTGTTCTCTAAATTAAGTAAACTTTCCATTTCTTTTTCAAAG
GAATATTTTATAGCAGCAGCATCAATTTTTTTAGATGGAATTAATTTTGGTTCTTTCGGAGTTGACGTTGTTTTTGAATT
TAAAATTTCTTCTCAAAGCTTGAATTTTGTTTCATTTTGTACGGATACTTTTGTTAAGAGATTAAAAAACAAATTATTGT
AA

Upstream 100 bases:

>100_bases
CAAACTTTAGATAGAAAATATATATCTGGAGTTGTAAAGCTTGACGAGGCTGATAATCTTGAGAGTGAATACTTAGTATT
AATTGATATAGCAAAAATTT

Downstream 100 bases:

>100_bases
GTTCAATGTTTTTTTAAACAGTGTTCTTTTTAATAAAAATTTTTCTTGGTTGTTGTTTGAATGTTTGGTTTAATCTAATC
TTTAATAAGGTTAGAGGATT

Product: hypothetical protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MINANLTTFPIKINMKNKVLLCINTLKSGASILGNDVKVYLETKYFVEVVLIDVGRPLFSFPKENFLFLITLGGDGTVLL
AVNLLLENENIDIPIISINMGNVGFLADIKIEDFKKVIDRFFNNSLVINKKFLLHVTVSQHGKDLISKYALNDIIIRSSV
LNKMIYVDLMVNSESFLSYKSDGIIVSTPTGSTGYSFSAGGPILEADLEGFXLTPISPHSVYNRSFVFSKLSKLSISFSK
EYFIAAASIFLDGINFGSFGVDVVFEFKISSQSLNFVSFCTDTFVKRLKNKLL

Sequences:

>Translated_293_residues
MINANLTTFPIKINMKNKVLLCINTLKSGASILGNDVKVYLETKYFVEVVLIDVGRPLFSFPKENFLFLITLGGDGTVLL
AVNLLLENENIDIPIISINMGNVGFLADIKIEDFKKVIDRFFNNSLVINKKFLLHVTVSQHGKDLISKYALNDIIIRSSV
LNKMIYVDLMVNSESFLSYKSDGIIVSTPTGSTGYSFSAGGPILEADLEGFXLTPISPHSVYNRSFVFSKLSKLSISFSK
EYFIAAASIFLDGINFGSFGVDVVFEFKISSQSLNFVSFCTDTFVKRLKNKLL
>Mature_293_residues
MINANLTTFPIKINMKNKVLLCINTLKSGASILGNDVKVYLETKYFVEVVLIDVGRPLFSFPKENFLFLITLGGDGTVLL
AVNLLLENENIDIPIISINMGNVGFLADIKIEDFKKVIDRFFNNSLVINKKFLLHVTVSQHGKDLISKYALNDIIIRSSV
LNKMIYVDLMVNSESFLSYKSDGIIVSTPTGSTGYSFSAGGPILEADLEGFXLTPISPHSVYNRSFVFSKLSKLSISFSK
EYFIAAASIFLDGINFGSFGVDVVFEFKISSQSLNFVSFCTDTFVKRLKNKLL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=172, Percent_Identity=34.3023255813954, Blast_Score=111, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320794, Length=187, Percent_Identity=29.9465240641711, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322509, Length=235, Percent_Identity=25.9574468085106, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325068, Length=195, Percent_Identity=30.2564102564103, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32485; Mature: 32485

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINANLTTFPIKINMKNKVLLCINTLKSGASILGNDVKVYLETKYFVEVVLIDVGRPLFS
CCCCCEEEEEEEEECCCEEEEEEECCCCCCHHCCCCEEEEEEEEEEEEEEEEECCCHHHH
FPKENFLFLITLGGDGTVLLAVNLLLENENIDIPIISINMGNVGFLADIKIEDFKKVIDR
CCCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEEEECCCEEEEEEEEHHHHHHHHHH
FFNNSLVINKKFLLHVTVSQHGKDLISKYALNDIIIRSSVLNKMIYVDLMVNSESFLSYK
HCCCEEEEEEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCHHHEEEC
SDGIIVSTPTGSTGYSFSAGGPILEADLEGFXLTPISPHSVYNRSFVFSKLSKLSISFSK
CCCEEEECCCCCCCCEECCCCCEEEECCCCEEECCCCCCHHCCHHHHHHHHHHEEEEECH
EYFIAAASIFLDGINFGSFGVDVVFEFKISSQSLNFVSFCTDTFVKRLKNKLL
HHHEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINANLTTFPIKINMKNKVLLCINTLKSGASILGNDVKVYLETKYFVEVVLIDVGRPLFS
CCCCCEEEEEEEEECCCEEEEEEECCCCCCHHCCCCEEEEEEEEEEEEEEEEECCCHHHH
FPKENFLFLITLGGDGTVLLAVNLLLENENIDIPIISINMGNVGFLADIKIEDFKKVIDR
CCCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEEEECCCEEEEEEEEHHHHHHHHHH
FFNNSLVINKKFLLHVTVSQHGKDLISKYALNDIIIRSSVLNKMIYVDLMVNSESFLSYK
HCCCEEEEEEEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCHHHEEEC
SDGIIVSTPTGSTGYSFSAGGPILEADLEGFXLTPISPHSVYNRSFVFSKLSKLSISFSK
CCCEEEECCCCCCCCEECCCCCEEEECCCCEEECCCCCCHHCCHHHHHHHHHHEEEEECH
EYFIAAASIFLDGINFGSFGVDVVFEFKISSQSLNFVSFCTDTFVKRLKNKLL
HHHEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]