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The map label for this gene is murF

Identifier: 15594649

GI number: 15594649

Start: 312729

End: 314123

Strand: Reverse

Name: murF

Synonym: BB0304

Alternate gene names: 15594649

Gene position: 314123-312729 (Counterclockwise)

Preceding gene: 15594650

Following gene: 15594648

Centisome position: 34.49

GC content: 28.32

Gene sequence:

>1395_bases
GTGCGTATAAAGATTAAGGATATTTTAATCTCTTCTAAAGATGTGAAATTTGTGGGGAATATAAAAAATATTGAAAGAGT
AGTGTCTTTTTATTCGTTAGATAGTCGCGAAATAAAGGATGACAATATCAATGATAGTCTTTATTTTGCGTATAAGGGAA
ATAAAGTAGATGGATTTTCTTTTGTTAAATATTTAATTGATCTGGGTGTTAAATGTTTTGCATGCTCAAGAGAGCATGAA
TCTGAGTGTATTAAATATTTAAATGACAATGAAGGGTTGGTTTTTTTGCTTACAAGCAATGTAATAAAACTTCTTCAAGC
TTTGGCATCGTTTTTAATTGAAAAAACAAGCTTTAAAAGAATTGCTATTACGGGTAGCAATGGCAAAACTACAACTAAAG
AGATGCTTTACAGTATACTTTCAAAGAAATACAAAACTTACAAAACTTGGGGTAATTTAAATTCTGATATTGGACTTCCT
CTTAGTATTTTAAGGGTAGAGGGTAATGAAGAATATGCTGTTTTTGAAGTTGGAGTTAGTTATGTTGGAGAAATGGATCT
TTTATCTCAAATTTTAAAACCAGAAATTGTTATTATTACGAATATAAGTTATGCGCATATGCAAGCCTTCAAGGAGTTGC
AAGCTATTGCTTTTGAAAAAAGCAAAATAATTGGCAAAAACATTGAAATCTTTGTTGTAAATGAAATGAATGATTATTGT
GTTTATCTTGAAAAAAGAGCAAAAATCGCAAATCCAAATGTTAAAATCGTTTATTTTGATTTTGAAAATCTTAGTATTAA
GTCATTTTCTTTTTTGGATGGGAAATTTTCTTTTGATTTTGTTTACAAAGGGTTTGAATACTCTATTTTATTGCTGGGTC
GGCATAATATTTTTAATGCAATAGGGTGTATTAATTTGGCTTTATTTTTAGGAATGAGAGAAAAAGAAATAAAAGAGGGC
CTTATTGAAACTGCTTTTCAAAAGGGTAGAGCAGAAATTTTGACAAAAAATGGATATTTGATTTTAAACGACTCTTATAA
TGGCAATATGGGTTCTTTTATGGCCTTAAAAAATATGATTTTAGATCTTAATATCCAAAACAAAAAGTTTATAGTTCTTG
GGTCTTTTAAAGAGCTTGGGGAATTGGCATACAAAACTCACAAAGATTTAATTCAAGAGGCTATTTCAATGAATTTTGAT
AAAATTTTTCTAATTGGCGAAGAATTTTTAGATGTTAGGGATTCTGAGAATTTAGTTGAAAAGTGTTTATATTACTTTAG
CGAGTTTGATAAATTTATTGATTTTTTTTTAAAAAGCTTGGAACCTTCAGTTTTTATTGTCATTAAGGGCTCAAGGTTTA
ACAGGCTTGAGAGAATTTTAAATATATTTAGATGA

Upstream 100 bases:

>100_bases
AGAATAGAGAGTTTTTGTTTTGGAGAATTAAATTTGGAAAAAAAAGCTAATGAAGATATAAATATTTTTGTTGAGTAAAA
GATTTTAGAATGAGGTTTGA

Downstream 100 bases:

>100_bases
TAGTGGGGTTTTTATGTTTTACCTTTTAGGTTTGCGTTTGCTCAAATATATTACCTTTAGAATGGCTTATGCTACAATTT
TTGCATTTTTACTTTCTTTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MRIKIKDILISSKDVKFVGNIKNIERVVSFYSLDSREIKDDNINDSLYFAYKGNKVDGFSFVKYLIDLGVKCFACSREHE
SECIKYLNDNEGLVFLLTSNVIKLLQALASFLIEKTSFKRIAITGSNGKTTTKEMLYSILSKKYKTYKTWGNLNSDIGLP
LSILRVEGNEEYAVFEVGVSYVGEMDLLSQILKPEIVIITNISYAHMQAFKELQAIAFEKSKIIGKNIEIFVVNEMNDYC
VYLEKRAKIANPNVKIVYFDFENLSIKSFSFLDGKFSFDFVYKGFEYSILLLGRHNIFNAIGCINLALFLGMREKEIKEG
LIETAFQKGRAEILTKNGYLILNDSYNGNMGSFMALKNMILDLNIQNKKFIVLGSFKELGELAYKTHKDLIQEAISMNFD
KIFLIGEEFLDVRDSENLVEKCLYYFSEFDKFIDFFLKSLEPSVFIVIKGSRFNRLERILNIFR

Sequences:

>Translated_464_residues
MRIKIKDILISSKDVKFVGNIKNIERVVSFYSLDSREIKDDNINDSLYFAYKGNKVDGFSFVKYLIDLGVKCFACSREHE
SECIKYLNDNEGLVFLLTSNVIKLLQALASFLIEKTSFKRIAITGSNGKTTTKEMLYSILSKKYKTYKTWGNLNSDIGLP
LSILRVEGNEEYAVFEVGVSYVGEMDLLSQILKPEIVIITNISYAHMQAFKELQAIAFEKSKIIGKNIEIFVVNEMNDYC
VYLEKRAKIANPNVKIVYFDFENLSIKSFSFLDGKFSFDFVYKGFEYSILLLGRHNIFNAIGCINLALFLGMREKEIKEG
LIETAFQKGRAEILTKNGYLILNDSYNGNMGSFMALKNMILDLNIQNKKFIVLGSFKELGELAYKTHKDLIQEAISMNFD
KIFLIGEEFLDVRDSENLVEKCLYYFSEFDKFIDFFLKSLEPSVFIVIKGSRFNRLERILNIFR
>Mature_464_residues
MRIKIKDILISSKDVKFVGNIKNIERVVSFYSLDSREIKDDNINDSLYFAYKGNKVDGFSFVKYLIDLGVKCFACSREHE
SECIKYLNDNEGLVFLLTSNVIKLLQALASFLIEKTSFKRIAITGSNGKTTTKEMLYSILSKKYKTYKTWGNLNSDIGLP
LSILRVEGNEEYAVFEVGVSYVGEMDLLSQILKPEIVIITNISYAHMQAFKELQAIAFEKSKIIGKNIEIFVVNEMNDYC
VYLEKRAKIANPNVKIVYFDFENLSIKSFSFLDGKFSFDFVYKGFEYSILLLGRHNIFNAIGCINLALFLGMREKEIKEG
LIETAFQKGRAEILTKNGYLILNDSYNGNMGSFMALKNMILDLNIQNKKFIVLGSFKELGELAYKTHKDLIQEAISMNFD
KIFLIGEEFLDVRDSENLVEKCLYYFSEFDKFIDFFLKSLEPSVFIVIKGSRFNRLERILNIFR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=456, Percent_Identity=25.4385964912281, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_BORBU (Q44777)

Other databases:

- EMBL:   AE000783
- EMBL:   U43739
- PIR:   H70137
- RefSeq:   NP_212438.1
- ProteinModelPortal:   Q44777
- SMR:   Q44777
- EnsemblBacteria:   EBBORT00000009056
- GeneID:   1195141
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0304
- NMPDR:   fig|224326.1.peg.688
- TIGR:   BB_0304
- GeneTree:   EBGT00050000006939
- HOGENOM:   HBG352886
- OMA:   MEAGISQ
- ProtClustDB:   CLSK507501
- BioCyc:   BBUR224326:BB_0304-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 53447; Mature: 53447

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKIKDILISSKDVKFVGNIKNIERVVSFYSLDSREIKDDNINDSLYFAYKGNKVDGFS
CEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
FVKYLIDLGVKCFACSREHESECIKYLNDNEGLVFLLTSNVIKLLQALASFLIEKTSFKR
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCE
IAITGSNGKTTTKEMLYSILSKKYKTYKTWGNLNSDIGLPLSILRVEGNEEYAVFEVGVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECHH
YVGEMDLLSQILKPEIVIITNISYAHMQAFKELQAIAFEKSKIIGKNIEIFVVNEMNDYC
HHCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
VYLEKRAKIANPNVKIVYFDFENLSIKSFSFLDGKFSFDFVYKGFEYSILLLGRHNIFNA
EEEEHHCEECCCCEEEEEEECCCCCEEEEEEECCCEEEEEEECCCEEEEEEEECCHHHHH
IGCINLALFLGMREKEIKEGLIETAFQKGRAEILTKNGYLILNDSYNGNMGSFMALKNMI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHH
LDLNIQNKKFIVLGSFKELGELAYKTHKDLIQEAISMNFDKIFLIGEEFLDVRDSENLVE
EEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHH
KCLYYFSEFDKFIDFFLKSLEPSVFIVIKGSRFNRLERILNIFR
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHC
>Mature Secondary Structure
MRIKIKDILISSKDVKFVGNIKNIERVVSFYSLDSREIKDDNINDSLYFAYKGNKVDGFS
CEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHH
FVKYLIDLGVKCFACSREHESECIKYLNDNEGLVFLLTSNVIKLLQALASFLIEKTSFKR
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCE
IAITGSNGKTTTKEMLYSILSKKYKTYKTWGNLNSDIGLPLSILRVEGNEEYAVFEVGVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECHH
YVGEMDLLSQILKPEIVIITNISYAHMQAFKELQAIAFEKSKIIGKNIEIFVVNEMNDYC
HHCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
VYLEKRAKIANPNVKIVYFDFENLSIKSFSFLDGKFSFDFVYKGFEYSILLLGRHNIFNA
EEEEHHCEECCCCEEEEEEECCCCCEEEEEEECCCEEEEEEECCCEEEEEEEECCHHHHH
IGCINLALFLGMREKEIKEGLIETAFQKGRAEILTKNGYLILNDSYNGNMGSFMALKNMI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHH
LDLNIQNKKFIVLGSFKELGELAYKTHKDLIQEAISMNFDKIFLIGEEFLDVRDSENLVE
EEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHH
KCLYYFSEFDKFIDFFLKSLEPSVFIVIKGSRFNRLERILNIFR
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685