Click here to switch to the map view.

The map label for this gene is pfpI [H]

Identifier: 154248187

GI number: 154248187

Start: 4734586

End: 4735167

Strand: Direct

Name: pfpI [H]

Synonym: Xaut_4267

Alternate gene names: 154248187

Gene position: 4734586-4735167 (Clockwise)

Preceding gene: 154248186

Following gene: 154248188

Centisome position: 89.18

GC content: 64.95

Gene sequence:

>582_bases
ATGGCGGGCAAGCGCATTTTGATGATCGTCGGCGATTTCGGCGAGGATTACGAGATCATGGTGCCCTACCAGGCGCTGCT
GGCGGTGGGGCATACGGTGCATGGCGTCTGCCCCGGCAAGAAGGCGGGCGACAAGATCGCCACCGCAATTCATGATTTCG
AGGGCCATCAAACCTACACGGAGAAGCCGGGCCACCTCTTTGCCCTCAACGCCACCTTCGCCGAGGTGGATGTTGCCAGC
TACGACGCCCTGCTGGTCCCCGGCGGTCGCGCGCCGGAATATCTGCGGCTCTATCCCGAGGTGATCGCCGCGGTCCGCCA
CTTCTTCGACGCGGGCAAGCCGGTGGCCGCCATCTGCCACGGCGCGCAGATGCTGGCTGCCGCAGGCGTCCTCAAGGGCC
GCACCTGCTCGGCCTATCCCGCCTGCCGCCCGGAAGTGGAGCTTGGCGGCGGCACCTATGCCGACATTCCCGTCGATACA
GCCGTCACCGAGGGCAACCTGGTGAGTGCGCCCGCATGGCCGGCCCATCCGGCCTTTATTGCGCAGTTCCTTACCCTATT
GGGAACATCCATCTCGCTTTGA

Upstream 100 bases:

>100_bases
GCTGTCGCAACCGGATAACACCCGGCGGGCGATGCGCGTCCTAACCTTGCCCCCACGACGCCGGCCGCGTTCATGCGCCG
GCGCAGCAGGGGAGAAGGCA

Downstream 100 bases:

>100_bases
GCCGCCCATCGGGTTATGGATGGCGCAGGGCGGCGAAACGGCATACGCGCAGCGCGGGGGTTGCGCGTATGCCGGCCGCC
CGGTTGATCTCATACCGTCG

Product: PfpI family intracellular peptidase

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MAGKRILMIVGDFGEDYEIMVPYQALLAVGHTVHGVCPGKKAGDKIATAIHDFEGHQTYTEKPGHLFALNATFAEVDVAS
YDALLVPGGRAPEYLRLYPEVIAAVRHFFDAGKPVAAICHGAQMLAAAGVLKGRTCSAYPACRPEVELGGGTYADIPVDT
AVTEGNLVSAPAWPAHPAFIAQFLTLLGTSISL

Sequences:

>Translated_193_residues
MAGKRILMIVGDFGEDYEIMVPYQALLAVGHTVHGVCPGKKAGDKIATAIHDFEGHQTYTEKPGHLFALNATFAEVDVAS
YDALLVPGGRAPEYLRLYPEVIAAVRHFFDAGKPVAAICHGAQMLAAAGVLKGRTCSAYPACRPEVELGGGTYADIPVDT
AVTEGNLVSAPAWPAHPAFIAQFLTLLGTSISL
>Mature_192_residues
AGKRILMIVGDFGEDYEIMVPYQALLAVGHTVHGVCPGKKAGDKIATAIHDFEGHQTYTEKPGHLFALNATFAEVDVASY
DALLVPGGRAPEYLRLYPEVIAAVRHFFDAGKPVAAICHGAQMLAAAGVLKGRTCSAYPACRPEVELGGGTYADIPVDTA
VTEGNLVSAPAWPAHPAFIAQFLTLLGTSISL

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=186, Percent_Identity=32.7956989247312, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20349; Mature: 20217

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKRILMIVGDFGEDYEIMVPYQALLAVGHTVHGVCPGKKAGDKIATAIHDFEGHQTYT
CCCCEEEEEEECCCCCCEEEECHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
EKPGHLFALNATFAEVDVASYDALLVPGGRAPEYLRLYPEVIAAVRHFFDAGKPVAAICH
CCCCEEEEEECCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GAQMLAAAGVLKGRTCSAYPACRPEVELGGGTYADIPVDTAVTEGNLVSAPAWPAHPAFI
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECCCCCCEECCCCEEECCCCCCCHHHH
AQFLTLLGTSISL
HHHHHHHHHCCCC
>Mature Secondary Structure 
AGKRILMIVGDFGEDYEIMVPYQALLAVGHTVHGVCPGKKAGDKIATAIHDFEGHQTYT
CCCEEEEEEECCCCCCEEEECHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
EKPGHLFALNATFAEVDVASYDALLVPGGRAPEYLRLYPEVIAAVRHFFDAGKPVAAICH
CCCCEEEEEECCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GAQMLAAAGVLKGRTCSAYPACRPEVELGGGTYADIPVDTAVTEGNLVSAPAWPAHPAFI
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECCCCCCEECCCCEEECCCCCCCHHHH
AQFLTLLGTSISL
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]