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The map label for this gene is puuE [H]

Identifier: 154248149

GI number: 154248149

Start: 4688788

End: 4690074

Strand: Direct

Name: puuE [H]

Synonym: Xaut_4229

Alternate gene names: 154248149

Gene position: 4688788-4690074 (Clockwise)

Preceding gene: 154248148

Following gene: 154248153

Centisome position: 88.32

GC content: 69.62

Gene sequence:

>1287_bases
ATGTCCGAGAATGCCGCGCTGCAAGCCCGCCGAACCAAGGCCATTCCCCGGGGCATCGCCACCGCCTATCCGGTCTTCGC
GCAGAAGGCCGAGGGGTCGGAGGTCTGGGACGCCGATGGCACGCGCTACATCGATTTCGCCGGCGGCATCGCCGTGCTCA
ACACCGGACACCGCCATCCCAGAGTGCTGGCGGCGGTGCGCGCCCAGCTCGACGCCTATACCCACACCGCGTTCCAGATC
GTGCCCTACGAGCCCTATGTGGCCCTGTGCGAGCGGCTGAACGCCATCGCCCCCTTCACCGGCGCAGCGAAATCCATCCT
GTTCTCCACCGGTGCCGAGGCGGTGGAGAACGCGGTGAAGATCGCCAAGGCCGCCACCGGCCGCACCGGTGTCATCTCCT
TTTCCGGCGGCTTCCACGGCCGCACGGCGCTGACCATGGCGCTCACGGGCAAGGTGCTGCCCTACAAGAAGAAGTTCGGC
ATCTCCCCGCCCGGCATCTTCCATCTCCCGTTCCCCTGCGCCGACCATGGCGTGAGCGTCGCCGACACGCTCAAGGCGCT
CGACTACCTGTTCCGCGCTGACATCGGCCCGCAGGACGTGGCGGCGATCATCATCGAGCCGGTGCAGGGCGAGGGCGGCT
TCCTGCCGGCGCCCACCGAGCTGCTTGTGGCCTTGCGCCAGACCTGCGACGCGCACGGCATCGTATTGATCGCCGACGAG
GTGCAGACCGGCTTTGCCCGCACCGGCCGCATGTTCGGCATCGAGCATTCCGGGGTCGAGCCGGATCTCGTCACCGTGGC
CAAGTCGCTGGCCGGCGGCTTTCCCCTGTCGGGCGTGATCGGTCGGGAGGCGATCATGGATGCCGCCGAGCCGGGCGGAC
TCGGCGGCACCTATGCCGGCAATCCCATCGCCTGCGCCGCCGCCCTCGCCGTGCTGGACGTGATCGCCGAGGAGAAGCTG
GTGGAGCGCGCCAACGCCATCGGCGCCCGCATCAAGGACGCCCTTACCCGCATGGCCCTGCGCAACGACGCCGTTGCCAT
CGGCTCCATCCGGGGGCCGGGCGCCATGGTCGCCTTCGACGTGGTGAAGAAGCGCGGCACCGTGGAGCCCGACGCCGACG
CCGCCCGGCGCGTGACCCAGGCGGCTCTGGCCGACGGGCTCATCCTCATCACCTGCGGCGTCTACGGCAATACCATCCGC
CTCCTGAACCCGCTGACCATCCCCGACGCGCTCCTCGATGAGGGCCTCGGCAAGCTGGAGCGGGCGCTGGTGGCAGGTGC
GGCCTGA

Upstream 100 bases:

>100_bases
TCGTCCGGTTGAGGCCGGCGGGGGCTGGTGGCACCGATCAATCCCATACCCGCGGAACGCGATCGCGTCTTATCCTGAGG
CAACCACAAGGAGGAGCGCC

Downstream 100 bases:

>100_bases
AGCTGGCGAGCTTGATGGATTCGTTGAAGCGGGCGGTTTCGGAAGGCCGGCCCGCTTCCTTGGCGCCTACAGTGCGCGGG
AGCCCGAAGCAGCGCTCAAG

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSENAALQARRTKAIPRGIATAYPVFAQKAEGSEVWDADGTRYIDFAGGIAVLNTGHRHPRVLAAVRAQLDAYTHTAFQI
VPYEPYVALCERLNAIAPFTGAAKSILFSTGAEAVENAVKIAKAATGRTGVISFSGGFHGRTALTMALTGKVLPYKKKFG
ISPPGIFHLPFPCADHGVSVADTLKALDYLFRADIGPQDVAAIIIEPVQGEGGFLPAPTELLVALRQTCDAHGIVLIADE
VQTGFARTGRMFGIEHSGVEPDLVTVAKSLAGGFPLSGVIGREAIMDAAEPGGLGGTYAGNPIACAAALAVLDVIAEEKL
VERANAIGARIKDALTRMALRNDAVAIGSIRGPGAMVAFDVVKKRGTVEPDADAARRVTQAALADGLILITCGVYGNTIR
LLNPLTIPDALLDEGLGKLERALVAGAA

Sequences:

>Translated_428_residues
MSENAALQARRTKAIPRGIATAYPVFAQKAEGSEVWDADGTRYIDFAGGIAVLNTGHRHPRVLAAVRAQLDAYTHTAFQI
VPYEPYVALCERLNAIAPFTGAAKSILFSTGAEAVENAVKIAKAATGRTGVISFSGGFHGRTALTMALTGKVLPYKKKFG
ISPPGIFHLPFPCADHGVSVADTLKALDYLFRADIGPQDVAAIIIEPVQGEGGFLPAPTELLVALRQTCDAHGIVLIADE
VQTGFARTGRMFGIEHSGVEPDLVTVAKSLAGGFPLSGVIGREAIMDAAEPGGLGGTYAGNPIACAAALAVLDVIAEEKL
VERANAIGARIKDALTRMALRNDAVAIGSIRGPGAMVAFDVVKKRGTVEPDADAARRVTQAALADGLILITCGVYGNTIR
LLNPLTIPDALLDEGLGKLERALVAGAA
>Mature_427_residues
SENAALQARRTKAIPRGIATAYPVFAQKAEGSEVWDADGTRYIDFAGGIAVLNTGHRHPRVLAAVRAQLDAYTHTAFQIV
PYEPYVALCERLNAIAPFTGAAKSILFSTGAEAVENAVKIAKAATGRTGVISFSGGFHGRTALTMALTGKVLPYKKKFGI
SPPGIFHLPFPCADHGVSVADTLKALDYLFRADIGPQDVAAIIIEPVQGEGGFLPAPTELLVALRQTCDAHGIVLIADEV
QTGFARTGRMFGIEHSGVEPDLVTVAKSLAGGFPLSGVIGREAIMDAAEPGGLGGTYAGNPIACAAALAVLDVIAEEKLV
ERANAIGARIKDALTRMALRNDAVAIGSIRGPGAMVAFDVVKKRGTVEPDADAARRVTQAALADGLILITCGVYGNTIRL
LNPLTIPDALLDEGLGKLERALVAGAA

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=416, Percent_Identity=30.7692307692308, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI13994255, Length=449, Percent_Identity=30.5122494432071, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI284507298, Length=327, Percent_Identity=32.1100917431193, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI37574042, Length=419, Percent_Identity=27.2076372315036, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI226442705, Length=418, Percent_Identity=26.555023923445, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24119277, Length=423, Percent_Identity=25.0591016548463, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI226442709, Length=387, Percent_Identity=27.6485788113695, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI188536080, Length=422, Percent_Identity=25.8293838862559, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI38679950, Length=422, Percent_Identity=25.8293838862559, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI38679946, Length=422, Percent_Identity=25.8293838862559, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787560, Length=423, Percent_Identity=58.3924349881797, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI1789016, Length=416, Percent_Identity=52.6442307692308, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1789759, Length=402, Percent_Identity=33.5820895522388, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788044, Length=404, Percent_Identity=32.1782178217822, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI145693181, Length=381, Percent_Identity=33.8582677165354, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786349, Length=352, Percent_Identity=30.1136363636364, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786991, Length=418, Percent_Identity=30.1435406698565, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564660, Length=431, Percent_Identity=32.0185614849188, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25144271, Length=399, Percent_Identity=31.8295739348371, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71992977, Length=443, Percent_Identity=28.8939051918736, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=252, Percent_Identity=33.7301587301587, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=472, Percent_Identity=25, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=402, Percent_Identity=30.8457711442786, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323470, Length=417, Percent_Identity=30.6954436450839, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324386, Length=449, Percent_Identity=22.0489977728285, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321456, Length=382, Percent_Identity=24.6073298429319, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357415, Length=416, Percent_Identity=30.0480769230769, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574759, Length=429, Percent_Identity=30.5361305361305, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI161085790, Length=445, Percent_Identity=30.1123595505618, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21356575, Length=421, Percent_Identity=29.6912114014252, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI281366494, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667139, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667143, Length=382, Percent_Identity=25.9162303664921, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44536; Mature: 44405

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENAALQARRTKAIPRGIATAYPVFAQKAEGSEVWDADGTRYIDFAGGIAVLNTGHRHP
CCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCEEEECCCCCCH
RVLAAVRAQLDAYTHTAFQIVPYEPYVALCERLNAIAPFTGAAKSILFSTGAEAVENAVK
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
IAKAATGRTGVISFSGGFHGRTALTMALTGKVLPYKKKFGISPPGIFHLPFPCADHGVSV
HHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEECCCCCCCCCCCH
ADTLKALDYLFRADIGPQDVAAIIIEPVQGEGGFLPAPTELLVALRQTCDAHGIVLIADE
HHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
VQTGFARTGRMFGIEHSGVEPDLVTVAKSLAGGFPLSGVIGREAIMDAAEPGGLGGTYAG
HHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
NPIACAAALAVLDVIAEEKLVERANAIGARIKDALTRMALRNDAVAIGSIRGPGAMVAFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
VVKKRGTVEPDADAARRVTQAALADGLILITCGVYGNTIRLLNPLTIPDALLDEGLGKLE
HHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
RALVAGAA
HHHHCCCC
>Mature Secondary Structure 
SENAALQARRTKAIPRGIATAYPVFAQKAEGSEVWDADGTRYIDFAGGIAVLNTGHRHP
CCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCEEEECCCCCCH
RVLAAVRAQLDAYTHTAFQIVPYEPYVALCERLNAIAPFTGAAKSILFSTGAEAVENAVK
HHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHH
IAKAATGRTGVISFSGGFHGRTALTMALTGKVLPYKKKFGISPPGIFHLPFPCADHGVSV
HHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEECCCCCCCCCCCH
ADTLKALDYLFRADIGPQDVAAIIIEPVQGEGGFLPAPTELLVALRQTCDAHGIVLIADE
HHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
VQTGFARTGRMFGIEHSGVEPDLVTVAKSLAGGFPLSGVIGREAIMDAAEPGGLGGTYAG
HHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
NPIACAAALAVLDVIAEEKLVERANAIGARIKDALTRMALRNDAVAIGSIRGPGAMVAFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
VVKKRGTVEPDADAARRVTQAALADGLILITCGVYGNTIRLLNPLTIPDALLDEGLGKLE
HHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHH
RALVAGAA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]