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The map label for this gene is pyrG

Identifier: 154248120

GI number: 154248120

Start: 4649754

End: 4651382

Strand: Direct

Name: pyrG

Synonym: Xaut_4200

Alternate gene names: 154248120

Gene position: 4649754-4651382 (Clockwise)

Preceding gene: 154248119

Following gene: 154248121

Centisome position: 87.58

GC content: 66.54

Gene sequence:

>1629_bases
ATGGCGCGCTATATCTTCATCACCGGCGGCGTCGTGTCTTCCCTGGGCAAGGGCCTCGCCGCCGCGGCTCTCGGCGCGCT
TCTCCAGGCGCGCGGCTATACCGTCCGGCTGCGCAAGCTGGACCCCTACCTCAACGTCGATCCGGGCACCATGAGCCCGT
ACCAGCACGGCGAGGTGTTCGTGACTGACGACGGGGCCGAGACCGATCTCGACCTCGGCCATTACGAGCGCTTCACCGGT
CATCCGGCGACGAAGCAGGACAACATCACCACCGGCCGCATCTATCAGGACATCCTGACCAAGGAGCGCCGCGGCGACTA
TCTCGGCGCCACCATCCAGGTCATCCCCCACGTCACCAATGCGATCAAGGACTTCGTCCTGGAAGGCAACGAGGGCTTCG
ACTTCGTGCTGATCGAGGTGGGCGGCACGGTGGGCGACATCGAGGGCCTGCCCTTCTTCGAAGCCACCCGCCAGCTGAAG
AACGAGCTGCCGCGCGGCCACGCCATCTTCATCCACCTCACCTTGATGCCCTATATCCCGTCCGCGGGCGAACTAAAGAC
CAAGCCGACCCAGCATTCGGTGAAGGAGTTGCGCTCCATCGGCATCCAGCCCGACATCCTGCTCTGCCGCACGGATCGCG
AGATTCCGCGCGAGGAGCGGCGCAAGCTCTCGCTGTTCTGCAACGTGCGCGAGACGGCGGTGATCGAGGCGCGGGACGTG
GACAACATCTATGCCGTCCCCTCCGCCTACCACGCGGAAGGCCTCGACAAGGAAGTGCTGGCCGCCTTCAACATCGAGCC
GGCACCGGCGCCCAACCTCAAGCGCTGGACCGACATCGAGGGGCGCGTGCGCAACCCGGAGGGCGAGGTCACCATCGCCA
TCGTGGGCAAATATACCGGCCTGAAGGACGCCTATAAGTCGCTGATCGAGGCGCTTGCCCATGGCGGCATCGCCCACAAG
GTGAAGGTCAATCTCGACTGGATCGAGAGCGAGACCTTCGAGCGCGAGGACCCCGCGCCGTTCCTGGAGCACGTGCACGG
CATCCTGGTGCCCGGCGGCTTCGGCCAGCGTGGCGCGGAAGGCAAGATCCGCGCGGCCCGCTTCGCCCGCGAGCGCAAGG
TGCCCTATCTCGGCATCTGCTTCGGCATGCAGATGGCGGTGATCGAGGCGGCGCGCAACCTTGCGGGCATCCCTGACGCC
AATTCCACGGAGTTCGGACCCACCAGCGAGCCGGTGGTGGGCCTGCTCACCGAATGGCTGAAGGGCAACGAGCTGGAGCG
CCGCAGCGCTGCCGGGGATCTGGGCGGCACCATGCGCCTCGGCGCCTATGCCGCGCATCTGGAGCCCGGCAGCCGGGTGG
AGGCGGTCTATGGCGGGCGCGACATCTTCGAGCGCCACCGCCACCGCTATGAGGTGAATACCGCCTATCGCCAACGCCTT
GAGCAGCATGGCCTGCGCTTCTCCGGCATGTCGCCGGACGGCCTCCTGCCGGAGATCGTGGAATATGGCGACCACCCCTG
GTTCATCGGGGTGCAGTTCCATCCGGAGCTGAAGTCGCGGCCGTTCCAGCCCCACCCGCTGTTCGCCTCGTTCGTGGGTG
CGGCCATGGAGCAGAGCCGGCTGGTGTGA

Upstream 100 bases:

>100_bases
CGCGATTCGCGCGGCGACATGCTTTCCCCACAGGCGAGGCGAGGCCGACACCGGCTTCGCCCTCGCCGAATCGCGTTTGC
ACGAGGTAAGGTCGAAGCCC

Downstream 100 bases:

>100_bases
CCTTCCGGCAGGGACAGCGGCAGGTATTCTCCTGCCGGTTGTTTCCCTCCCGGCCGCCGCCGCTCTAAGCTCCCCCCGGA
TGAGTCGGGGAGGGGAGTTG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARYIFITGGVVSSLGKGLAAAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
HPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLK
NELPRGHAIFIHLTLMPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPREERRKLSLFCNVRETAVIEARDV
DNIYAVPSAYHAEGLDKEVLAAFNIEPAPAPNLKRWTDIEGRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALAHGGIAHK
VKVNLDWIESETFEREDPAPFLEHVHGILVPGGFGQRGAEGKIRAARFARERKVPYLGICFGMQMAVIEAARNLAGIPDA
NSTEFGPTSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYAAHLEPGSRVEAVYGGRDIFERHRHRYEVNTAYRQRL
EQHGLRFSGMSPDGLLPEIVEYGDHPWFIGVQFHPELKSRPFQPHPLFASFVGAAMEQSRLV

Sequences:

>Translated_542_residues
MARYIFITGGVVSSLGKGLAAAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
HPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLK
NELPRGHAIFIHLTLMPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPREERRKLSLFCNVRETAVIEARDV
DNIYAVPSAYHAEGLDKEVLAAFNIEPAPAPNLKRWTDIEGRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALAHGGIAHK
VKVNLDWIESETFEREDPAPFLEHVHGILVPGGFGQRGAEGKIRAARFARERKVPYLGICFGMQMAVIEAARNLAGIPDA
NSTEFGPTSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYAAHLEPGSRVEAVYGGRDIFERHRHRYEVNTAYRQRL
EQHGLRFSGMSPDGLLPEIVEYGDHPWFIGVQFHPELKSRPFQPHPLFASFVGAAMEQSRLV
>Mature_541_residues
ARYIFITGGVVSSLGKGLAAAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGH
PATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTNAIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKN
ELPRGHAIFIHLTLMPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPREERRKLSLFCNVRETAVIEARDVD
NIYAVPSAYHAEGLDKEVLAAFNIEPAPAPNLKRWTDIEGRVRNPEGEVTIAIVGKYTGLKDAYKSLIEALAHGGIAHKV
KVNLDWIESETFEREDPAPFLEHVHGILVPGGFGQRGAEGKIRAARFARERKVPYLGICFGMQMAVIEAARNLAGIPDAN
STEFGPTSEPVVGLLTEWLKGNELERRSAAGDLGGTMRLGAYAAHLEPGSRVEAVYGGRDIFERHRHRYEVNTAYRQRLE
QHGLRFSGMSPDGLLPEIVEYGDHPWFIGVQFHPELKSRPFQPHPLFASFVGAAMEQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.7037701974865, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=555, Percent_Identity=44.1441441441441, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI28559083, Length=555, Percent_Identity=44.1441441441441, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI221316689, Length=555, Percent_Identity=44.1441441441441, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=59.1078066914498, Blast_Score=624, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=36.9281045751634, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=39.3298059964727, Blast_Score=415, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=44.0071556350626, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.970297029703, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60075; Mature: 59944

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00442 GATASE_TYPE_I ; PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLAAAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGHPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKNELPRGHAIFIHLTLMPYIP
HHHHHEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPREERRKLSLFCNVRETAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHEEEEEEECCHHEEEEECCC
DNIYAVPSAYHAEGLDKEVLAAFNIEPAPAPNLKRWTDIEGRVRNPEGEVTIAIVGKYTG
CCEEECCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCC
LKDAYKSLIEALAHGGIAHKVKVNLDWIESETFEREDPAPFLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCC
GKIRAARFARERKVPYLGICFGMQMAVIEAARNLAGIPDANSTEFGPTSEPVVGLLTEWL
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELERRSAAGDLGGTMRLGAYAAHLEPGSRVEAVYGGRDIFERHRHRYEVNTAYRQRL
CCCCHHHHHCCCCCCCCEECCEEEEECCCCCCEEEEECCHHHHHHHHHEEECCHHHHHHH
EQHGLRFSGMSPDGLLPEIVEYGDHPWFIGVQFHPELKSRPFQPHPLFASFVGAAMEQSR
HHCCCEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLAAAALGALLQARGYTVRLRKLDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGHPATKQDNITTGRIYQDILTKERRGDYLGATIQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFVLEGNEGFDFVLIEVGGTVGDIEGLPFFEATRQLKNELPRGHAIFIHLTLMPYIP
HHHHHEECCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPREERRKLSLFCNVRETAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHEEEEEEECCHHEEEEECCC
DNIYAVPSAYHAEGLDKEVLAAFNIEPAPAPNLKRWTDIEGRVRNPEGEVTIAIVGKYTG
CCEEECCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCC
LKDAYKSLIEALAHGGIAHKVKVNLDWIESETFEREDPAPFLEHVHGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCC
GKIRAARFARERKVPYLGICFGMQMAVIEAARNLAGIPDANSTEFGPTSEPVVGLLTEWL
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KGNELERRSAAGDLGGTMRLGAYAAHLEPGSRVEAVYGGRDIFERHRHRYEVNTAYRQRL
CCCCHHHHHCCCCCCCCEECCEEEEECCCCCCEEEEECCHHHHHHHHHEEECCHHHHHHH
EQHGLRFSGMSPDGLLPEIVEYGDHPWFIGVQFHPELKSRPFQPHPLFASFVGAAMEQSR
HHCCCEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]