BacMap

Definition	Xanthobacter autotrophicus Py2 chromosome, complete genome.
Accession	NC_009720
Length	5,308,934

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The map label for this gene is braF [H]

Identifier: 154248073

GI number: 154248073

Start: 4595703

End: 4596563

Strand: Direct

Name: braF [H]

Synonym: Xaut_4152

Alternate gene names: 154248073

Gene position: 4595703-4596563 (Clockwise)

Preceding gene: 154248072

Following gene: 154248074

Centisome position: 86.57

GC content: 66.55

Gene sequence:

>861_bases
ATGAGCAGCTTTCAGACCCAGCCCTCCGACCCCTATGTGGTGAAGTCCCACGGCGCCCTGGCGCTGGAAGCGGAGATGCG
GGAGATCGCGCAGGATCGCCCCGGCGCCGACCGGATGTCCATGACGCCGGCATTGCTCTATCTCGACAACATCACCGTCT
CCTTCGACGGCTTCCGCGCGCTGAACGCCCTTTCCCTCGTCATCGGGGAAGCGGAGATGCGGGCCATCATCGGCCCCAAC
GGCGCCGGCAAGACCACCATGATGGACGTGATCACCGGCAAGACGCGGCCGAACCAGGGCGTGGCCTATTTCGCCGGCAC
CCACGACCTCACCAGCCTGGACGAGGCCGCCATCGCCCAGCTCGGCATCGGCCGCAAGTTCCAGACGCCCACCGTGTTCG
ACATGCATTCGGTGGAGGACAACATCCTCCTCGCCTTGAAGGAGGACCGGCGGCCCTTCTCCAACATCTACTGGCGGCGC
ACGCCCCACGAGCGCGAGCGCATCGACGAATTGCTGGAGCGGGTGCGCCTGAAGGATGCGCGTTTCCGCCGCGCCGGCGA
TCTCTCCCACGGCCAGAAGCAATGGCTGGAGATCGGCATGCTGCTGGCGCAGGAGCCGCGCCTGTTGCTGGTGGACGAGC
CCGCCGCCGGCATGACCGACCAGGAAACGGCGGACACGGCGGTGCTGCTGCGCGAGATCGCCAAGACGCGATCCGTGGTG
GTGGTGGAGCACGACATGCAGTTCGTGCGCGATCTCGACGTGAAGGTCACCGTGCTGCACGAAGGCTCCGTGCTGGCCGA
AGGCCCGCTCGACCAGGTGAGCGCCGACGAACGGGTCATCGAAGTCTATCTGGGGAGGTGA

Upstream 100 bases:

>100_bases
TGCCCCGGGGCGTGCTGGGCCTTGCCTCCAGCATTTCCGAACGCTGGAAGGCGCGGCGGCCCGCTGCGACGCCCACCCCC
GAGCCGGCGGAGTGAGCGCC

Downstream 100 bases:

>100_bases
GGGCATGCTGAAGGTTGAGAACCTCGACCTCTATTACGGCGCCGCCCACACCCTGAAGAAGGTGTCGGTGGAAGCCCATG
CGGGCAAGGTCACCTGCGTG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSSFQTQPSDPYVVKSHGALALEAEMREIAQDRPGADRMSMTPALLYLDNITVSFDGFRALNALSLVIGEAEMRAIIGPN
GAGKTTMMDVITGKTRPNQGVAYFAGTHDLTSLDEAAIAQLGIGRKFQTPTVFDMHSVEDNILLALKEDRRPFSNIYWRR
TPHERERIDELLERVRLKDARFRRAGDLSHGQKQWLEIGMLLAQEPRLLLVDEPAAGMTDQETADTAVLLREIAKTRSVV
VVEHDMQFVRDLDVKVTVLHEGSVLAEGPLDQVSADERVIEVYLGR

Sequences:

>Translated_286_residues
MSSFQTQPSDPYVVKSHGALALEAEMREIAQDRPGADRMSMTPALLYLDNITVSFDGFRALNALSLVIGEAEMRAIIGPN
GAGKTTMMDVITGKTRPNQGVAYFAGTHDLTSLDEAAIAQLGIGRKFQTPTVFDMHSVEDNILLALKEDRRPFSNIYWRR
TPHERERIDELLERVRLKDARFRRAGDLSHGQKQWLEIGMLLAQEPRLLLVDEPAAGMTDQETADTAVLLREIAKTRSVV
VVEHDMQFVRDLDVKVTVLHEGSVLAEGPLDQVSADERVIEVYLGR
>Mature_285_residues
SSFQTQPSDPYVVKSHGALALEAEMREIAQDRPGADRMSMTPALLYLDNITVSFDGFRALNALSLVIGEAEMRAIIGPNG
AGKTTMMDVITGKTRPNQGVAYFAGTHDLTSLDEAAIAQLGIGRKFQTPTVFDMHSVEDNILLALKEDRRPFSNIYWRRT
PHERERIDELLERVRLKDARFRRAGDLSHGQKQWLEIGMLLAQEPRLLLVDEPAAGMTDQETADTAVLLREIAKTRSVVV
VEHDMQFVRDLDVKVTVLHEGSVLAEGPLDQVSADERVIEVYLGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27262624, Length=207, Percent_Identity=28.9855072463768, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI27262626, Length=207, Percent_Identity=28.9855072463768, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45446740, Length=251, Percent_Identity=25.8964143426295, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI47078218, Length=251, Percent_Identity=25.8964143426295, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21536376, Length=224, Percent_Identity=24.5535714285714, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27881501, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI30795238, Length=218, Percent_Identity=25.2293577981651, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31657092, Length=226, Percent_Identity=25.2212389380531, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27436953, Length=244, Percent_Identity=26.6393442622951, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116734710, Length=211, Percent_Identity=27.4881516587678, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI153792144, Length=209, Percent_Identity=25.3588516746411, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI27477115, Length=196, Percent_Identity=29.0816326530612, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI105990541, Length=212, Percent_Identity=24.0566037735849, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI150417984, Length=221, Percent_Identity=27.1493212669683, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI42741659, Length=293, Percent_Identity=23.2081911262799, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=32.2834645669291, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790190, Length=244, Percent_Identity=31.1475409836066, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790525, Length=249, Percent_Identity=28.9156626506024, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082268, Length=250, Percent_Identity=32, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081791, Length=246, Percent_Identity=28.4552845528455, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787089, Length=232, Percent_Identity=29.3103448275862, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786398, Length=237, Percent_Identity=28.6919831223629, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788472, Length=256, Percent_Identity=29.296875, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789593, Length=243, Percent_Identity=27.9835390946502, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790739, Length=231, Percent_Identity=26.8398268398268, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=31.4410480349345, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787792, Length=234, Percent_Identity=26.0683760683761, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790162, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789991, Length=248, Percent_Identity=27.8225806451613, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788225, Length=243, Percent_Identity=27.5720164609054, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788897, Length=237, Percent_Identity=26.5822784810127, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081834, Length=235, Percent_Identity=29.3617021276596, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786345, Length=238, Percent_Identity=27.3109243697479, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789672, Length=241, Percent_Identity=25.3112033195021, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786872, Length=239, Percent_Identity=26.3598326359833, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081782, Length=227, Percent_Identity=27.3127753303965, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787548, Length=240, Percent_Identity=25.4166666666667, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081709, Length=223, Percent_Identity=26.0089686098655, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790544, Length=248, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787370, Length=233, Percent_Identity=27.4678111587983, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786803, Length=248, Percent_Identity=22.5806451612903, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48995001, Length=223, Percent_Identity=26.0089686098655, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786563, Length=232, Percent_Identity=28.0172413793103, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789873, Length=224, Percent_Identity=25.8928571428571, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790467, Length=231, Percent_Identity=26.4069264069264, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=238, Percent_Identity=27.7310924369748, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788210, Length=234, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787712, Length=221, Percent_Identity=24.4343891402715, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994943, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786698, Length=224, Percent_Identity=24.5535714285714, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=24.1228070175439, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789586, Length=241, Percent_Identity=28.2157676348548, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788450, Length=237, Percent_Identity=26.1603375527426, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787759, Length=254, Percent_Identity=27.9527559055118, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082267, Length=223, Percent_Identity=26.457399103139, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788506, Length=251, Percent_Identity=27.8884462151394, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789963, Length=243, Percent_Identity=27.5720164609054, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=227, Percent_Identity=29.5154185022026, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71983523, Length=244, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=244, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=244, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17565586, Length=206, Percent_Identity=25.2427184466019, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=216, Percent_Identity=27.3148148148148, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558664, Length=219, Percent_Identity=25.5707762557078, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543740, Length=229, Percent_Identity=24.4541484716157, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24581191, Length=203, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581189, Length=203, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581193, Length=203, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581195, Length=203, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221512771, Length=224, Percent_Identity=26.7857142857143, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24643674, Length=223, Percent_Identity=26.457399103139, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28574744, Length=190, Percent_Identity=27.3684210526316, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85724864, Length=178, Percent_Identity=26.4044943820225, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24580555, Length=178, Percent_Identity=26.4044943820225, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI85816269, Length=178, Percent_Identity=26.4044943820225, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24580553, Length=178, Percent_Identity=26.4044943820225, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17136662, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31910; Mature: 31779

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFQTQPSDPYVVKSHGALALEAEMREIAQDRPGADRMSMTPALLYLDNITVSFDGFRA
CCCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEECEEEEECCHHH
LNALSLVIGEAEMRAIIGPNGAGKTTMMDVITGKTRPNQGVAYFAGTHDLTSLDEAAIAQ
HHHHHHHHCHHHEEEEECCCCCCCCEEEEEHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LGIGRKFQTPTVFDMHSVEDNILLALKEDRRPFSNIYWRRTPHERERIDELLERVRLKDA
HCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RFRRAGDLSHGQKQWLEIGMLLAQEPRLLLVDEPAAGMTDQETADTAVLLREIAKTRSVV
HHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
VVEHDMQFVRDLDVKVTVLHEGSVLAEGPLDQVSADERVIEVYLGR
EEECCHHHHHCCCEEEEEEECCCEEECCCCHHCCCCCEEEEEEECC
>Mature Secondary Structure 
SSFQTQPSDPYVVKSHGALALEAEMREIAQDRPGADRMSMTPALLYLDNITVSFDGFRA
CCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEECEEEEECCHHH
LNALSLVIGEAEMRAIIGPNGAGKTTMMDVITGKTRPNQGVAYFAGTHDLTSLDEAAIAQ
HHHHHHHHCHHHEEEEECCCCCCCCEEEEEHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LGIGRKFQTPTVFDMHSVEDNILLALKEDRRPFSNIYWRRTPHERERIDELLERVRLKDA
HCCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHH
RFRRAGDLSHGQKQWLEIGMLLAQEPRLLLVDEPAAGMTDQETADTAVLLREIAKTRSVV
HHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
VVEHDMQFVRDLDVKVTVLHEGSVLAEGPLDQVSADERVIEVYLGR
EEECCHHHHHCCCEEEEEEECCCEEECCCCHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]