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The map label for this gene is livJ [C]

Identifier: 154247981

GI number: 154247981

Start: 4493511

End: 4494713

Strand: Direct

Name: livJ [C]

Synonym: Xaut_4060

Alternate gene names: 154247981

Gene position: 4493511-4494713 (Clockwise)

Preceding gene: 154247977

Following gene: 154247982

Centisome position: 84.64

GC content: 67.41

Gene sequence:

>1203_bases
ATGCCTCCACAGAGCATTTTGCGCAGCCAGCTTCTGGCCGCGGCGGTGGCGCTCGGCCTTTCCACCGGCCTTTCGATCCC
CGCGGCGCTGGCCGATCAGGAACCCTGCATCGGCAATTCCGCCGCCGTGACCGGCCCGGCGGCGTTCTCCGGCCAGGCCA
TCAAGATGGGCGCGGAGATCGCCATCGAGGAGATCAACGCGGCCGGCGGCGTGCTCGGCAAGAAGCTGCGGCTGGTGCAG
TATGACGATGCCGGCGCCCCGCCGCGCGGCGTCGACAACACCCGCCGCATCGCGCTGGCGGACAAGTGCATCGCCGTGCT
CGGCGGCTTCCATTCCACCGTCGCCTTGGCGCAGGTGGACCCGGTGCACGAGATCGGCATTCCCTACATGGGCGTGTGGG
CGGCCAACACCAAGGCCATCGAGAACGGTCGCGATCCCAACTTCATGTTCCGCGTCTCCGCCAAGGACAAGTGGGTGGCC
CGCTTCCTGGTGGACGAAGCGCTCAAGGTGTCCAAGACCGGCAAGGTGGCATTCTTCTACGAGAACACCGGCTGGGGCAA
CGGCGCGCTGCCGGACGTGAAGGCCGCGCTCGCCGCCAGGGGCAAGGAACTGGTGGCCGCGGAAACCTTCAACTGGAACG
ACCAGGACATGTCGCCCCAGGTGATCCGCGCCCGCGATGCCGGGGCCGACGTGGCCATCATCTGGTCGCTGGACCGCGAG
GCCAACCAGATCCTGCGCTCCATGGACAAGGCGAGCTGGAAGCCGGCCATCGTCGGCGCCTGGGGCATCTCCGGCAATCT
CGGCGAGCTGGCCGGCCCCCTCGCCAACGGGGTGCGGGTGATGCAGACCTATTCCTTCCTGACCCCCCAGAGCGAGGCCG
GCAAGAAGCTGCTGGCCGCCGTCGAGGCCAAGACCGGCATCAAGGACCCCAAGGACATCAAGGCGCCCTCGGGCATCGCC
AATTCCTATGACGCGGTCTACGTGCTGGCCGACGCCATCAAGATCGCCGGCTCGTTTGACTGGAAGAAGGTGCAGCAGGC
CCTCTACCAGGTCAGCCGCGACGGCCTCGTCGCCCCCTACAAGCCGGCCTTCGACAAGGCCGATCCCGAGCGCCAGGACG
CCATCCTGCCGCAATACTACCTGCTGACCGTCTGGCACGACGGCAAGCTGATCCCCCTCAAGGGCTCGCCCTACGACAAG
TGA

Upstream 100 bases:

>100_bases
TAATCTTTGTCCTGAGGCTGCGCCTATATCAGCCTTATCTCGCAGATGATTTTCGGGCGGACATGCCCGGCACGTCCTGT
ACACGGCAGGAGAAACAGAC

Downstream 100 bases:

>100_bases
GCCCGCGCGGGAGGCGGTTTCCCTCGCCGCCTCCCGCTTTTTCTCGCCCACTCTGCCACCACTCGCCTCGACCGGCCACG
GCCGGGCCCCCCCCTCCGAC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPPQSILRSQLLAAAVALGLSTGLSIPAALADQEPCIGNSAAVTGPAAFSGQAIKMGAEIAIEEINAAGGVLGKKLRLVQ
YDDAGAPPRGVDNTRRIALADKCIAVLGGFHSTVALAQVDPVHEIGIPYMGVWAANTKAIENGRDPNFMFRVSAKDKWVA
RFLVDEALKVSKTGKVAFFYENTGWGNGALPDVKAALAARGKELVAAETFNWNDQDMSPQVIRARDAGADVAIIWSLDRE
ANQILRSMDKASWKPAIVGAWGISGNLGELAGPLANGVRVMQTYSFLTPQSEAGKKLLAAVEAKTGIKDPKDIKAPSGIA
NSYDAVYVLADAIKIAGSFDWKKVQQALYQVSRDGLVAPYKPAFDKADPERQDAILPQYYLLTVWHDGKLIPLKGSPYDK

Sequences:

>Translated_400_residues
MPPQSILRSQLLAAAVALGLSTGLSIPAALADQEPCIGNSAAVTGPAAFSGQAIKMGAEIAIEEINAAGGVLGKKLRLVQ
YDDAGAPPRGVDNTRRIALADKCIAVLGGFHSTVALAQVDPVHEIGIPYMGVWAANTKAIENGRDPNFMFRVSAKDKWVA
RFLVDEALKVSKTGKVAFFYENTGWGNGALPDVKAALAARGKELVAAETFNWNDQDMSPQVIRARDAGADVAIIWSLDRE
ANQILRSMDKASWKPAIVGAWGISGNLGELAGPLANGVRVMQTYSFLTPQSEAGKKLLAAVEAKTGIKDPKDIKAPSGIA
NSYDAVYVLADAIKIAGSFDWKKVQQALYQVSRDGLVAPYKPAFDKADPERQDAILPQYYLLTVWHDGKLIPLKGSPYDK
>Mature_399_residues
PPQSILRSQLLAAAVALGLSTGLSIPAALADQEPCIGNSAAVTGPAAFSGQAIKMGAEIAIEEINAAGGVLGKKLRLVQY
DDAGAPPRGVDNTRRIALADKCIAVLGGFHSTVALAQVDPVHEIGIPYMGVWAANTKAIENGRDPNFMFRVSAKDKWVAR
FLVDEALKVSKTGKVAFFYENTGWGNGALPDVKAALAARGKELVAAETFNWNDQDMSPQVIRARDAGADVAIIWSLDREA
NQILRSMDKASWKPAIVGAWGISGNLGELAGPLANGVRVMQTYSFLTPQSEAGKKLLAAVEAKTGIKDPKDIKAPSGIAN
SYDAVYVLADAIKIAGSFDWKKVQQALYQVSRDGLVAPYKPAFDKADPERQDAILPQYYLLTVWHDGKLIPLKGSPYDK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=301, Percent_Identity=26.9102990033223, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789867, Length=331, Percent_Identity=26.2839879154079, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42654; Mature: 42523

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPQSILRSQLLAAAVALGLSTGLSIPAALADQEPCIGNSAAVTGPAAFSGQAIKMGAEI
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEECCHHH
AIEEINAAGGVLGKKLRLVQYDDAGAPPRGVDNTRRIALADKCIAVLGGFHSTVALAQVD
HHHHHHHHCCHHHCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHEEEEECC
PVHEIGIPYMGVWAANTKAIENGRDPNFMFRVSAKDKWVARFLVDEALKVSKTGKVAFFY
CHHHHCCCEEEEEECCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEE
ENTGWGNGALPDVKAALAARGKELVAAETFNWNDQDMSPQVIRARDAGADVAIIWSLDRE
ECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCCCCEEEEEECCHH
ANQILRSMDKASWKPAIVGAWGISGNLGELAGPLANGVRVMQTYSFLTPQSEAGKKLLAA
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VEAKTGIKDPKDIKAPSGIANSYDAVYVLADAIKIAGSFDWKKVQQALYQVSRDGLVAPY
HHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
KPAFDKADPERQDAILPQYYLLTVWHDGKLIPLKGSPYDK
CCCCCCCCCCHHHCCCCCEEEEEEEECCEEEEECCCCCCC
>Mature Secondary Structure 
PPQSILRSQLLAAAVALGLSTGLSIPAALADQEPCIGNSAAVTGPAAFSGQAIKMGAEI
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEECCHHH
AIEEINAAGGVLGKKLRLVQYDDAGAPPRGVDNTRRIALADKCIAVLGGFHSTVALAQVD
HHHHHHHHCCHHHCEEEEEEECCCCCCCCCCCCCCEEHHHHHHHHHHCCHHHHEEEEECC
PVHEIGIPYMGVWAANTKAIENGRDPNFMFRVSAKDKWVARFLVDEALKVSKTGKVAFFY
CHHHHCCCEEEEEECCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEE
ENTGWGNGALPDVKAALAARGKELVAAETFNWNDQDMSPQVIRARDAGADVAIIWSLDRE
ECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHEEECCCCCCEEEEEECCHH
ANQILRSMDKASWKPAIVGAWGISGNLGELAGPLANGVRVMQTYSFLTPQSEAGKKLLAA
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VEAKTGIKDPKDIKAPSGIANSYDAVYVLADAIKIAGSFDWKKVQQALYQVSRDGLVAPY
HHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCC
KPAFDKADPERQDAILPQYYLLTVWHDGKLIPLKGSPYDK
CCCCCCCCCCHHHCCCCCEEEEEEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]