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The map label for this gene is yfcG [H]

Identifier: 154247722

GI number: 154247722

Start: 4203431

End: 4204141

Strand: Direct

Name: yfcG [H]

Synonym: Xaut_3798

Alternate gene names: 154247722

Gene position: 4203431-4204141 (Clockwise)

Preceding gene: 154247721

Following gene: 154247723

Centisome position: 79.18

GC content: 64.56

Gene sequence:

>711_bases
ATGGCCGCAGTCCAGTCTTTGCCGATCGAGCTTTTCTACTGGCCCACCCCCAACGGATGGAAGATCACCATCATGCTGGA
GGAATGCGGGCTGCCCTACGAGGTCAAGCTCGTGAACATCGGCAAGGGCGACCAGTTCAAGCCGGACTTCCTCGCCATCT
CGCCCAACAACAAGATGCCGGCCATCATCGATCCGGACGGGCCGGGCGGGCAGCCCATCTCGGTGTTCGAATCCGGGGCC
ATCCTCCAGTATCTCGGCCGCAAGACCGGCCTGTTCTATCCCGCTGACGAGCGCGGCCGGGTGGAAGTGGACCAGTGGCT
GTTCTGGCAGATGGGAGGCCTTGGCCCCATGGCCGGGCAGGCGCACCATTTCCGCATCTACGCGCCGGAGAAGATCCCCT
ATGCCGTTGAGCGCTACACCAACGAGGTGCACCGGCTCTACGGCGTCATGAACACCCGCCTGAAGGACCGTGAATACCTT
GCCGGCGACTATTCCATCGCCGATATCGCCTGCGTGGGCTGGGCCAAGCTGTGGGAGCGGCAGGGCCAGAACATCGAGGA
GTTTCCCCACTTCAAGGCGTGGCTGGGCCGCGTCCTGGCACGTCCGGCGGTGCAGCGGGGCCTGGCCGTAAATGCCGAGG
AGCGGGCCAAGGTAGACCTCTCCAGGGACAAGGAGGCCCAGAGCGTCCTCTTTGGCCAGCGCGCCCGCTGA

Upstream 100 bases:

>100_bases
TACGCTGCGGCCGCGGGACCGGCCTCTGACACGAAGATGAGAGTCCGCCTGACCTCGGGATCGCCAAACGATCCGAGGGA
GACGGCTCTGGGAGCGCATC

Downstream 100 bases:

>100_bases
TCTCCCGTCTGCCATCAAGCCCGCGTGTGAGCCGGCAATGCTATAAGGGTGGTTCTGACTGATTCCGCGGCAAGGAGCGC
CCATGGCCGAGGACGAGCGC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MAAVQSLPIELFYWPTPNGWKITIMLEECGLPYEVKLVNIGKGDQFKPDFLAISPNNKMPAIIDPDGPGGQPISVFESGA
ILQYLGRKTGLFYPADERGRVEVDQWLFWQMGGLGPMAGQAHHFRIYAPEKIPYAVERYTNEVHRLYGVMNTRLKDREYL
AGDYSIADIACVGWAKLWERQGQNIEEFPHFKAWLGRVLARPAVQRGLAVNAEERAKVDLSRDKEAQSVLFGQRAR

Sequences:

>Translated_236_residues
MAAVQSLPIELFYWPTPNGWKITIMLEECGLPYEVKLVNIGKGDQFKPDFLAISPNNKMPAIIDPDGPGGQPISVFESGA
ILQYLGRKTGLFYPADERGRVEVDQWLFWQMGGLGPMAGQAHHFRIYAPEKIPYAVERYTNEVHRLYGVMNTRLKDREYL
AGDYSIADIACVGWAKLWERQGQNIEEFPHFKAWLGRVLARPAVQRGLAVNAEERAKVDLSRDKEAQSVLFGQRAR
>Mature_235_residues
AAVQSLPIELFYWPTPNGWKITIMLEECGLPYEVKLVNIGKGDQFKPDFLAISPNNKMPAIIDPDGPGGQPISVFESGAI
LQYLGRKTGLFYPADERGRVEVDQWLFWQMGGLGPMAGQAHHFRIYAPEKIPYAVERYTNEVHRLYGVMNTRLKDREYLA
GDYSIADIACVGWAKLWERQGQNIEEFPHFKAWLGRVLARPAVQRGLAVNAEERAKVDLSRDKEAQSVLFGQRAR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=202, Percent_Identity=54.4554455445545, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI87082195, Length=222, Percent_Identity=40.990990990991, Blast_Score=148, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324100, Length=248, Percent_Identity=36.2903225806452, Blast_Score=135, Evalue=6e-33,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=28.1609195402299, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864598, Length=192, Percent_Identity=30.7291666666667, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17933730, Length=211, Percent_Identity=28.436018957346, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17864592, Length=193, Percent_Identity=26.9430051813472, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24646259, Length=200, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221378983, Length=200, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45549270, Length=191, Percent_Identity=26.1780104712042, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI85725204, Length=191, Percent_Identity=27.2251308900524, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737923, Length=191, Percent_Identity=27.2251308900524, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864594, Length=191, Percent_Identity=25.6544502617801, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646249, Length=192, Percent_Identity=26.0416666666667, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646251, Length=177, Percent_Identity=26.5536723163842, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26738; Mature: 26607

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVQSLPIELFYWPTPNGWKITIMLEECGLPYEVKLVNIGKGDQFKPDFLAISPNNKMP
CCCCCCCCEEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCC
AIIDPDGPGGQPISVFESGAILQYLGRKTGLFYPADERGRVEVDQWLFWQMGGLGPMAGQ
EEECCCCCCCCEEHHHCCCHHHHHHCCCCCEECCCCCCCCEEHHHEEEECCCCCCCCCCC
AHHFRIYAPEKIPYAVERYTNEVHRLYGVMNTRLKDREYLAGDYSIADIACVGWAKLWER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
QGQNIEEFPHFKAWLGRVLARPAVQRGLAVNAEERAKVDLSRDKEAQSVLFGQRAR
CCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHCEECCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
AAVQSLPIELFYWPTPNGWKITIMLEECGLPYEVKLVNIGKGDQFKPDFLAISPNNKMP
CCCCCCCEEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCEEEECCCCCCC
AIIDPDGPGGQPISVFESGAILQYLGRKTGLFYPADERGRVEVDQWLFWQMGGLGPMAGQ
EEECCCCCCCCEEHHHCCCHHHHHHCCCCCEECCCCCCCCEEHHHEEEECCCCCCCCCCC
AHHFRIYAPEKIPYAVERYTNEVHRLYGVMNTRLKDREYLAGDYSIADIACVGWAKLWER
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
QGQNIEEFPHFKAWLGRVLARPAVQRGLAVNAEERAKVDLSRDKEAQSVLFGQRAR
CCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHCEECCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]