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The map label for this gene is insK [C]

Identifier: 154247661

GI number: 154247661

Start: 4141413

End: 4142285

Strand: Direct

Name: insK [C]

Synonym: Xaut_3736

Alternate gene names: 154247661

Gene position: 4141413-4142285 (Clockwise)

Preceding gene: 154247660

Following gene: 154247667

Centisome position: 78.01

GC content: 67.24

Gene sequence:

>873_bases
ATGAGCGAACGGCGGGCGTGTAAAGCCACCGGCTTCTGCCGCATGACCATGAGATACCAAGCGAAGCGGGCCGACGACGG
CGCCCTGCGCCAGCGCATGAAGGCCATCGCCCACGAGCGGCGGCGCTTCGGCTATCGGCGTCTGCACGTGCTGCTCAGGC
GGGAGGGCTTCACGGTCAACCACAAGCGCCTGTTCCGGCTCTATCGCGAGGAGCGCCTCAAGGTGCGGCGCCGTGGTGGC
CGCAAGCGGGCCATCGGGACACGGGCGCCCATGACGATCCCGATGCGGCCGAACGAGCGCTGGTCGCTCGACTTCGTGTC
GGACCAGTTCACCTGCGGCCGCCGCTTCCGCATCCTCACCGTTGTGGATGACTGCACCCGGGAATGCCTCACCCTGGTGG
CGGACACATCGCTGTCCGGCGTCCGTGTCGCGCGCGAACTCGATCGCCTTGTGGCCGAGCGTGGCAGGCCGAAAATGATC
GTCAGCGACAACGGCAGCGAGTTCACCTCGAATGCCATCCTCGCCTGGGCGGACGCGGCACAAGTCGAGTGGCACTACAT
CGCGCCGGGCAAGCCCATGCAGAACGCCTTCATCGAGAGCTTCAACGGCCGCCTGCGCGACGAGCTTCTCAACGAAACGC
TGTTCTCGGCGCTCACCCAAGCCAGAGCGGCCCTGAGCAGGTGGCGGTCCGACTACAATGGAGCGCGCCCCCACTCCGCC
CTCGGCTGGCAGACCCCGTCCGCCTTCGCAGCCACCTTCCACCCGCGACGGGATCTGCCGCTGCGCCAGGCCAAAAGCTC
CGCGCCAGATCCCGCCGCTCACCCGGCCGACAAGGCCAGATCCAACCGTCAGAACGAACTCACCGTTGGATAA

Upstream 100 bases:

>100_bases
CGATGCTGGACAATGTGGCGCTGAAGGATCTCCTGGGAAAGAAGTGGTGACGCCCGCTGCCGAGCGCAAAGCCGTCGCCC
ATCTCATGGGGCACCACGGG

Downstream 100 bases:

>100_bases
AACTTGGGGGCAACGTCACGTCCTAAGGCGGATATTTTCTCTACAATAACCCGGTGACAGAGATGTGGTACGAATAAGGT
TGTTCAGAACTAGAGTCTGA

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSERRACKATGFCRMTMRYQAKRADDGALRQRMKAIAHERRRFGYRRLHVLLRREGFTVNHKRLFRLYREERLKVRRRGG
RKRAIGTRAPMTIPMRPNERWSLDFVSDQFTCGRRFRILTVVDDCTRECLTLVADTSLSGVRVARELDRLVAERGRPKMI
VSDNGSEFTSNAILAWADAAQVEWHYIAPGKPMQNAFIESFNGRLRDELLNETLFSALTQARAALSRWRSDYNGARPHSA
LGWQTPSAFAATFHPRRDLPLRQAKSSAPDPAAHPADKARSNRQNELTVG

Sequences:

>Translated_290_residues
MSERRACKATGFCRMTMRYQAKRADDGALRQRMKAIAHERRRFGYRRLHVLLRREGFTVNHKRLFRLYREERLKVRRRGG
RKRAIGTRAPMTIPMRPNERWSLDFVSDQFTCGRRFRILTVVDDCTRECLTLVADTSLSGVRVARELDRLVAERGRPKMI
VSDNGSEFTSNAILAWADAAQVEWHYIAPGKPMQNAFIESFNGRLRDELLNETLFSALTQARAALSRWRSDYNGARPHSA
LGWQTPSAFAATFHPRRDLPLRQAKSSAPDPAAHPADKARSNRQNELTVG
>Mature_289_residues
SERRACKATGFCRMTMRYQAKRADDGALRQRMKAIAHERRRFGYRRLHVLLRREGFTVNHKRLFRLYREERLKVRRRGGR
KRAIGTRAPMTIPMRPNERWSLDFVSDQFTCGRRFRILTVVDDCTRECLTLVADTSLSGVRVARELDRLVAERGRPKMIV
SDNGSEFTSNAILAWADAAQVEWHYIAPGKPMQNAFIESFNGRLRDELLNETLFSALTQARAALSRWRSDYNGARPHSAL
GWQTPSAFAATFHPRRDLPLRQAKSSAPDPAAHPADKARSNRQNELTVG

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=270, Percent_Identity=26.2962962962963, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790724, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789424, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789224, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788305, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787669, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786558, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786462, Length=157, Percent_Identity=28.6624203821656, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33341; Mature: 33209

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERRACKATGFCRMTMRYQAKRADDGALRQRMKAIAHERRRFGYRRLHVLLRREGFTVN
CCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKRLFRLYREERLKVRRRGGRKRAIGTRAPMTIPMRPNERWSLDFVSDQFTCGRRFRILT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEEEEE
VVDDCTRECLTLVADTSLSGVRVARELDRLVAERGRPKMIVSDNGSEFTSNAILAWADAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCEEEEECCC
QVEWHYIAPGKPMQNAFIESFNGRLRDELLNETLFSALTQARAALSRWRSDYNGARPHSA
EEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LGWQTPSAFAATFHPRRDLPLRQAKSSAPDPAAHPADKARSNRQNELTVG
CCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure 
SERRACKATGFCRMTMRYQAKRADDGALRQRMKAIAHERRRFGYRRLHVLLRREGFTVN
CCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HKRLFRLYREERLKVRRRGGRKRAIGTRAPMTIPMRPNERWSLDFVSDQFTCGRRFRILT
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCEEEEEE
VVDDCTRECLTLVADTSLSGVRVARELDRLVAERGRPKMIVSDNGSEFTSNAILAWADAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCEEEEECCC
QVEWHYIAPGKPMQNAFIESFNGRLRDELLNETLFSALTQARAALSRWRSDYNGARPHSA
EEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LGWQTPSAFAATFHPRRDLPLRQAKSSAPDPAAHPADKARSNRQNELTVG
CCCCCCCHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]