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The map label for this gene is rna [C]

Identifier: 154246670

GI number: 154246670

Start: 3025554

End: 3026216

Strand: Reverse

Name: rna [C]

Synonym: Xaut_2731

Alternate gene names: 154246670

Gene position: 3026216-3025554 (Counterclockwise)

Preceding gene: 154246671

Following gene: 154246669

Centisome position: 57.0

GC content: 69.08

Gene sequence:

>663_bases
GTGAGGCGCGCCCTCTTCGCCCTTACCCTGTTCGCTGGTCTGCTCGTCGCCGCTTTGCCGGCGGCGGCGCAGGGGCATGA
GCCCGGCCGGTTTGACTTCTACGTGCTGTCGCTGTCCTGGTCGCCCACCTATTGCGAGGACAAGGGCCAGAAGGCGGCCA
ACGAGCCCCAGTGCAACCTTACCCGCCCCTACGCCTTCGTGGTCCACGGCCTGTGGCCGCAATACGAGCGCGGCTTTCCG
CAGGACTGCCAGCGCCCGCCGCCCTATGTGCCCAATCCGCTGGTGAGAAGCATGCTGGACGTGATGCCGAGCCGGGGGCT
CGTCATCTATGAGTGGAAGAAGCACGGTACCTGCTCCGGCCTCTCGCCCCAGGCCTATTTCGACCTCGTGCGCGCGGCGC
GGGACAAGGTGGCCATCCCCGCCGAGTTCACCCGGCTCGACGACTACCGCATGGTCTCGCCCTCCGACGTGGAAAGCGCC
TTCCGGGCCGCCAATCCCGGCCTGTCGCCGGACATGTTCTCGGTGCAGTGCGACCGCCGCCGGCTGACCGAGGTGCGCAT
CTGCCTCAGCCAGGATCTCGCCTTCCGGGCCTGCCCCGACGTGGACCGCCGCGCCTGCCGCCTGTCGCGCATGGTGATGC
CGCCCGTGCGCGGCGGCAAATGA

Upstream 100 bases:

>100_bases
AGGACGGCGGCTTCATCGGCGCCAGCTGGGGCGTGGATTGGTGCGTGGTCGGCCTCGACCGCAATTATGCCTATGCCCCG
TCCTGCAAGATGGCGGGTCC

Downstream 100 bases:

>100_bases
GGCGGCTAGGGCCCGTTTCGGCGATTGCCCCACATGGGCCCTTGCAGACCGCGGCAACGCATTGCCATCGTCATGGCCGG
GCTTGTCCCGGCCATCCACG

Product: ribonuclease T2

Products: 3'- Phosphomononucleotides; 3'-Phosphooligonucleotides [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MRRALFALTLFAGLLVAALPAAAQGHEPGRFDFYVLSLSWSPTYCEDKGQKAANEPQCNLTRPYAFVVHGLWPQYERGFP
QDCQRPPPYVPNPLVRSMLDVMPSRGLVIYEWKKHGTCSGLSPQAYFDLVRAARDKVAIPAEFTRLDDYRMVSPSDVESA
FRAANPGLSPDMFSVQCDRRRLTEVRICLSQDLAFRACPDVDRRACRLSRMVMPPVRGGK

Sequences:

>Translated_220_residues
MRRALFALTLFAGLLVAALPAAAQGHEPGRFDFYVLSLSWSPTYCEDKGQKAANEPQCNLTRPYAFVVHGLWPQYERGFP
QDCQRPPPYVPNPLVRSMLDVMPSRGLVIYEWKKHGTCSGLSPQAYFDLVRAARDKVAIPAEFTRLDDYRMVSPSDVESA
FRAANPGLSPDMFSVQCDRRRLTEVRICLSQDLAFRACPDVDRRACRLSRMVMPPVRGGK
>Mature_220_residues
MRRALFALTLFAGLLVAALPAAAQGHEPGRFDFYVLSLSWSPTYCEDKGQKAANEPQCNLTRPYAFVVHGLWPQYERGFP
QDCQRPPPYVPNPLVRSMLDVMPSRGLVIYEWKKHGTCSGLSPQAYFDLVRAARDKVAIPAEFTRLDDYRMVSPSDVESA
FRAANPGLSPDMFSVQCDRRRLTEVRICLSQDLAFRACPDVDRRACRLSRMVMPPVRGGK

Specific function: One of the few RNases that cleave the phosphodiester bond between any two nucleotide. Shows a preference for adenylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=An RNase I-like form (periplasmic) and RNase I*-like form (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17562468, Length=213, Percent_Identity=24.8826291079812, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: 3.1.27.6 [C]

Molecular weight: Translated: 24786; Mature: 24786

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALFALTLFAGLLVAALPAAAQGHEPGRFDFYVLSLSWSPTYCEDKGQKAANEPQCNL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
TRPYAFVVHGLWPQYERGFPQDCQRPPPYVPNPLVRSMLDVMPSRGLVIYEWKKHGTCSG
CCCHHEEHCCCCCHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCC
LSPQAYFDLVRAARDKVAIPAEFTRLDDYRMVSPSDVESAFRAANPGLSPDMFSVQCDRR
CCHHHHHHHHHHHHCCCCCCHHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHH
RLTEVRICLSQDLAFRACPDVDRRACRLSRMVMPPVRGGK
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRALFALTLFAGLLVAALPAAAQGHEPGRFDFYVLSLSWSPTYCEDKGQKAANEPQCNL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
TRPYAFVVHGLWPQYERGFPQDCQRPPPYVPNPLVRSMLDVMPSRGLVIYEWKKHGTCSG
CCCHHEEHCCCCCHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCC
LSPQAYFDLVRAARDKVAIPAEFTRLDDYRMVSPSDVESAFRAANPGLSPDMFSVQCDRR
CCHHHHHHHHHHHHCCCCCCHHHHHCCCEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHH
RLTEVRICLSQDLAFRACPDVDRRACRLSRMVMPPVRGGK
HHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nucleotides [C]

Specific reaction: Endonucleolytic Cleavage With 2',3'-Cyclic Phosphate Intermediates. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685334 [H]