BacMap

Definition	Xanthobacter autotrophicus Py2 chromosome, complete genome.
Accession	NC_009720
Length	5,308,934

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The map label for this gene is dkgB [H]

Identifier: 154246656

GI number: 154246656

Start: 3011673

End: 3012497

Strand: Reverse

Name: dkgB [H]

Synonym: Xaut_2717

Alternate gene names: 154246656

Gene position: 3012497-3011673 (Counterclockwise)

Preceding gene: 154246657

Following gene: 154246655

Centisome position: 56.74

GC content: 67.76

Gene sequence:

>825_bases
ATGCATAATGTGACCGCCAATGGCGCTGAGATCCCCGCCCTCGGCTTCGGCACCTTCCGCGTCTCGGCGGCTGATACCCT
GCGCATGGTGCCCGCCGCCCTGAAGCTCGGCTTCCGCCACATCGACACCGCGCAGATCTACGGCAACGAGGCCGAGGTGG
GCGAAGGCATTGCGTCGTCCGGCGTGAAGCGGGGCGACATCTTCCTCACCACCAAGGTGTGGGTGAGCAACTATGCCCAC
GACGCCTTCCTCGCCTCGGTGGACGAGAGCCTGAAGAAGCTGAAGACGGACTATGTGGACCTGCTGCTGCTGCACTGGCC
CAACCCGGCGGTGCCCCTGGCCGAGCAGATCGGCGCGCTCAATGCGGTGCGGGCCGCCGGCAAGGTGCGCCACATGGGCG
TGTCCAACTTCAACACCGCGCTGATGGCGGAGGCGATCGCCCTGTCCGACGGGCCGCTGGTGACCAACCAGATCGAGTTC
CACCCCTATATCGACCAGTCGAAGGTGATCCGGGCTGCCCGCGCCGCCGGTCTCTCCCTCACCGCCTATTACGCCATGGC
GGATGGCAAGGTGTTCGCCGATCCGGTGTTGAAGGACATCGCCGCCGCCCACAAGCGCACGGTGGCGCAGGTGGTGCTGC
GCTGGGTGGTGCAGCAGGAGGGCTTCGTCGCCCTGTCCAAGACCGTGAGCGAGGCCCGCGCCGCCGAGAATGCCGCCATC
TTCGACTTCGCGCTCACCGAGGCGGAGATGGCCGCCATCTGCGCCCTCGCCCACCCGGACGGGCGCATCGTCAGCCCCGA
GGGGCTGGCGCCTGTGTGGGACTGA

Upstream 100 bases:

>100_bases
CCCTCATGGTGGCCGGAGCGCTGGGGCTGACGGTTTGGAGCGCCGCATTGGACAACCGCGCCCGCGCTGTCGCCTGCCCC
GTCTGAACAAAGGAAACAGC

Downstream 100 bases:

>100_bases
GCGAGGGGGGCGGCGGCTTCGGCCTCCCCTTCCCTACGCGGGTAGGTCCGGAGGGCGGCGGGATTCGCAATGGCGCTTTA
TCGGCGCTCGTCTGCCGGCT

Product: aldo/keto reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MHNVTANGAEIPALGFGTFRVSAADTLRMVPAALKLGFRHIDTAQIYGNEAEVGEGIASSGVKRGDIFLTTKVWVSNYAH
DAFLASVDESLKKLKTDYVDLLLLHWPNPAVPLAEQIGALNAVRAAGKVRHMGVSNFNTALMAEAIALSDGPLVTNQIEF
HPYIDQSKVIRAARAAGLSLTAYYAMADGKVFADPVLKDIAAAHKRTVAQVVLRWVVQQEGFVALSKTVSEARAAENAAI
FDFALTEAEMAAICALAHPDGRIVSPEGLAPVWD

Sequences:

>Translated_274_residues
MHNVTANGAEIPALGFGTFRVSAADTLRMVPAALKLGFRHIDTAQIYGNEAEVGEGIASSGVKRGDIFLTTKVWVSNYAH
DAFLASVDESLKKLKTDYVDLLLLHWPNPAVPLAEQIGALNAVRAAGKVRHMGVSNFNTALMAEAIALSDGPLVTNQIEF
HPYIDQSKVIRAARAAGLSLTAYYAMADGKVFADPVLKDIAAAHKRTVAQVVLRWVVQQEGFVALSKTVSEARAAENAAI
FDFALTEAEMAAICALAHPDGRIVSPEGLAPVWD
>Mature_274_residues
MHNVTANGAEIPALGFGTFRVSAADTLRMVPAALKLGFRHIDTAQIYGNEAEVGEGIASSGVKRGDIFLTTKVWVSNYAH
DAFLASVDESLKKLKTDYVDLLLLHWPNPAVPLAEQIGALNAVRAAGKVRHMGVSNFNTALMAEAIALSDGPLVTNQIEF
HPYIDQSKVIRAARAAGLSLTAYYAMADGKVFADPVLKDIAAAHKRTVAQVVLRWVVQQEGFVALSKTVSEARAAENAAI
FDFALTEAEMAAICALAHPDGRIVSPEGLAPVWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=271, Percent_Identity=36.5313653136531, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI310109920, Length=290, Percent_Identity=35.1724137931034, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI45446745, Length=290, Percent_Identity=35.1724137931034, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503285, Length=290, Percent_Identity=35.1724137931034, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5453543, Length=287, Percent_Identity=34.8432055749129, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI223468663, Length=287, Percent_Identity=33.1010452961672, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=34.1379310344828, Blast_Score=157, Evalue=8e-39,
Organism=Homo sapiens, GI24497585, Length=290, Percent_Identity=33.448275862069, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI24497577, Length=283, Percent_Identity=30.0353356890459, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI5174391, Length=283, Percent_Identity=30.0353356890459, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=33.5616438356164, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI291291012, Length=285, Percent_Identity=32.6315789473684, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI93277124, Length=297, Percent_Identity=30.3030303030303, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI4502049, Length=284, Percent_Identity=33.4507042253521, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=31.1594202898551, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI300116271, Length=270, Percent_Identity=31.8518518518519, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI310109926, Length=121, Percent_Identity=41.3223140495868, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI207028673, Length=121, Percent_Identity=41.3223140495868, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=35.1351351351351, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=44.1947565543071, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082198, Length=264, Percent_Identity=32.9545454545455, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1788081, Length=278, Percent_Identity=31.6546762589928, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI48994888, Length=253, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081735, Length=146, Percent_Identity=29.4520547945205, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789375, Length=308, Percent_Identity=22.0779220779221, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17561298, Length=268, Percent_Identity=32.8358208955224, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=32.1917808219178, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550248, Length=280, Percent_Identity=33.9285714285714, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=31.2925170068027, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=30.03300330033, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=30.9433962264151, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=28.3582089552239, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=33.7037037037037, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17564128, Length=289, Percent_Identity=30.1038062283737, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17566692, Length=284, Percent_Identity=29.2253521126761, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17561300, Length=266, Percent_Identity=28.9473684210526, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17562292, Length=272, Percent_Identity=26.8382352941176, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324694, Length=291, Percent_Identity=34.3642611683849, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320576, Length=290, Percent_Identity=35.1724137931034, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322556, Length=266, Percent_Identity=32.7067669172932, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321896, Length=300, Percent_Identity=29, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319625, Length=287, Percent_Identity=30.3135888501742, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320079, Length=279, Percent_Identity=29.0322580645161, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24657054, Length=295, Percent_Identity=34.5762711864407, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24663317, Length=281, Percent_Identity=34.5195729537367, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24662789, Length=296, Percent_Identity=30.4054054054054, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24644950, Length=292, Percent_Identity=31.8493150684932, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21356425, Length=279, Percent_Identity=32.258064516129, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI221378297, Length=312, Percent_Identity=30.1282051282051, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI20129731, Length=294, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24662785, Length=276, Percent_Identity=34.0579710144928, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=29.8181818181818, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281366140, Length=249, Percent_Identity=28.5140562248996, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29277; Mature: 29277

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNVTANGAEIPALGFGTFRVSAADTLRMVPAALKLGFRHIDTAQIYGNEAEVGEGIASS
CCCCCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHCHHCCCHHHEECCHHHHCCCHHHC
GVKRGDIFLTTKVWVSNYAHDAFLASVDESLKKLKTDYVDLLLLHWPNPAVPLAEQIGAL
CCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHH
NAVRAAGKVRHMGVSNFNTALMAEAIALSDGPLVTNQIEFHPYIDQSKVIRAARAAGLSL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECEEEECCCCCHHHHHHHHHHCCCEE
TAYYAMADGKVFADPVLKDIAAAHKRTVAQVVLRWVVQQEGFVALSKTVSEARAAENAAI
EEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
FDFALTEAEMAAICALAHPDGRIVSPEGLAPVWD
EEHHHHHHHHHHHHEEECCCCCEECCCCCCCCCC
>Mature Secondary Structure
MHNVTANGAEIPALGFGTFRVSAADTLRMVPAALKLGFRHIDTAQIYGNEAEVGEGIASS
CCCCCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHCHHCCCHHHEECCHHHHCCCHHHC
GVKRGDIFLTTKVWVSNYAHDAFLASVDESLKKLKTDYVDLLLLHWPNPAVPLAEQIGAL
CCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHH
NAVRAAGKVRHMGVSNFNTALMAEAIALSDGPLVTNQIEFHPYIDQSKVIRAARAAGLSL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEECEEEECCCCCHHHHHHHHHHCCCEE
TAYYAMADGKVFADPVLKDIAAAHKRTVAQVVLRWVVQQEGFVALSKTVSEARAAENAAI
EEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
FDFALTEAEMAAICALAHPDGRIVSPEGLAPVWD
EEHHHHHHHHHHHHEEECCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]