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The map label for this gene is higA-1 [H]

Identifier: 154246589

GI number: 154246589

Start: 2944392

End: 2944700

Strand: Reverse

Name: higA-1 [H]

Synonym: Xaut_2649

Alternate gene names: 154246589

Gene position: 2944700-2944392 (Counterclockwise)

Preceding gene: 154246590

Following gene: 154246585

Centisome position: 55.47

GC content: 65.05

Gene sequence:

>309_bases
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAAATCCTCACCGAAGAGTTCATGCAGCCGATGGGGTTGACGCA
GGGTGCGCTCGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTCAACGAACTGTGCGGCAACCGCAGAAACGTGACGGCGG
CGACGGCCCTGATCCTGGCCCGTGTGTTCGGCAACAGCCCCGACTTCTGGCTCAACGTGCAGCGGCGCAGCGATCTTTGG
GAGGTGATGAACACGCCGAAGGAGCGCGAGCGGATCAAGCGCGCACGCCCGTTGCAGAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
AACCAGCAATGGCGGTTGATCTTCCGCTGGGATGGCGTGCGCGGCGAGGCGGACGGAATTTATCTGGACGATCACAGCTA
CCGATGAAAGCGAGGCAAAG

Downstream 100 bases:

>100_bases
GTCATGCGACCTCACCGCTCGGGCCGGAACAACAGTTGAGAAGGAGGCATTGCCCATGACCGTACCGCAGCGCGCCGCTC
TCTATCTGCGCGTCTCGACG

Product: XRE family plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 102

Protein sequence:

>102_residues
MLTTKRKPATVGEILTEEFMQPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
EVMNTPKERERIKRARPLQSAA

Sequences:

>Translated_102_residues
MLTTKRKPATVGEILTEEFMQPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
EVMNTPKERERIKRARPLQSAA
>Mature_102_residues
MLTTKRKPATVGEILTEEFMQPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
EVMNTPKERERIKRARPLQSAA

Specific function: Antitoxin that counteracts the effect of the higB-1 toxin. Binds to its own promoter and regulates transcription of the higB-1/higA-1 operon [H]

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=89, Percent_Identity=43.8202247191011, Blast_Score=81, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 11521; Mature: 11521

Theoretical pI: Translated: 11.17; Mature: 11.17

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTKRKPATVGEILTEEFMQPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILA
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RVFGNSPDFWLNVQRRSDLWEVMNTPKERERIKRARPLQSAA
HHHCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTTKRKPATVGEILTEEFMQPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILA
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RVFGNSPDFWLNVQRRSDLWEVMNTPKERERIKRARPLQSAA
HHHCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]