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The map label for this gene is ynfL [H]

Identifier: 154246514

GI number: 154246514

Start: 2863908

End: 2864870

Strand: Reverse

Name: ynfL [H]

Synonym: Xaut_2573

Alternate gene names: 154246514

Gene position: 2864870-2863908 (Counterclockwise)

Preceding gene: 154246522

Following gene: 154246513

Centisome position: 53.96

GC content: 68.85

Gene sequence:

>963_bases
ATGAACGTGTCCCTGCGCGCCCTGCGCTACGTGGTGGCCGCGGCCGATTGCGGCAACGTCACCGAGGCGGCACGCCAGCT
CAACGTGTCCCAGCCGTCCATCTCCGCCGCCATCGCCCAGACCGAGGCGGACCTCAAGCTGCAACTGTTCGTCCGCCACC
ATGCGCGAGGCATCTCGCTCACCTCCGCCGGGCAGCGCTATGTGAACGAGGCGCGCCATCTTCTCGCCCACGCCCGCGAC
TTCGCCCAGACCGCCGAGGCGCTGGGGGACAGCCTGAGGGGGGAAATCGCGCTCGGCTGCTTCCTCACCATCGCCCCTCG
CTTCATGCCAGCGCTGCTGGCGCGCTTCGCCGAGGTCCAGCCGGGCATCACGGTGAAGCTGGAGGAAGGTGACCAGCGGG
AGATTCTCGACGGCCTGCTCTCCGGCCGGCTGGAGATTGCCGTGTCCTACGGCTACGCGGTGCCCGAGGAATTGTCAGGT
GAACTGCTCATCGACCTGCCGCCGCTGCTGGTGGTGGCGGCCGATCATCCTTTGGCGGGCAAGAGCGTGGTCTCGCTGCA
GGACGTGGCGGACGAGCCCTTCCTGCTCTACGACCTGCCCCACACCCGCGACTATTTCTTCTCCCTGTTCGATGCCGCCG
GCATCACCCCGCGCATCGCCTATCGCTCGCGCTCCTATGAGCTGATCCGGGGCCTGGTGGGGCAGAAGCGCGGCTACACG
CTGCACAATGCCTCGCCGCGCACCACCATGGCCTATGACGGCAGCCGGGTGGCGGTGCGGCCGCTAAAGGAGCGCCTGCC
GCCGGTGAAGGTGATGCGCCTCACCCTGCGCCGCCAGCCCATGCGCCCGGCGGTGGAGGTCTTCGCCCGCTTTCTTTCGG
ATGCCTTCTCCCCCGGCGGCATGTTCGCCCCCGGCTCCATCGCGCCGGACATGGATGCCCGGAGCCGCGACCACCTGGCG
TAA

Upstream 100 bases:

>100_bases
ATCATCGGAGCCCTGTCGACGTGGTCGTGTCCGCCCCCTGCCCGCCTTCGAAATTGTCGTTTCTGTCCAGCGCCCTGGTT
CGGCCGGAAGCCCGCCGGCC

Downstream 100 bases:

>100_bases
ATGTCCTTGGGGATGTCGGAACGCCCGCCGGCATTCGTCCTTGGGGGGATGCAGGCCGCCACGGCCAGCGCAAACCAGGG
AGAGCGACATGCGCGTGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNVSLRALRYVVAAADCGNVTEAARQLNVSQPSISAAIAQTEADLKLQLFVRHHARGISLTSAGQRYVNEARHLLAHARD
FAQTAEALGDSLRGEIALGCFLTIAPRFMPALLARFAEVQPGITVKLEEGDQREILDGLLSGRLEIAVSYGYAVPEELSG
ELLIDLPPLLVVAADHPLAGKSVVSLQDVADEPFLLYDLPHTRDYFFSLFDAAGITPRIAYRSRSYELIRGLVGQKRGYT
LHNASPRTTMAYDGSRVAVRPLKERLPPVKVMRLTLRRQPMRPAVEVFARFLSDAFSPGGMFAPGSIAPDMDARSRDHLA

Sequences:

>Translated_320_residues
MNVSLRALRYVVAAADCGNVTEAARQLNVSQPSISAAIAQTEADLKLQLFVRHHARGISLTSAGQRYVNEARHLLAHARD
FAQTAEALGDSLRGEIALGCFLTIAPRFMPALLARFAEVQPGITVKLEEGDQREILDGLLSGRLEIAVSYGYAVPEELSG
ELLIDLPPLLVVAADHPLAGKSVVSLQDVADEPFLLYDLPHTRDYFFSLFDAAGITPRIAYRSRSYELIRGLVGQKRGYT
LHNASPRTTMAYDGSRVAVRPLKERLPPVKVMRLTLRRQPMRPAVEVFARFLSDAFSPGGMFAPGSIAPDMDARSRDHLA
>Mature_320_residues
MNVSLRALRYVVAAADCGNVTEAARQLNVSQPSISAAIAQTEADLKLQLFVRHHARGISLTSAGQRYVNEARHLLAHARD
FAQTAEALGDSLRGEIALGCFLTIAPRFMPALLARFAEVQPGITVKLEEGDQREILDGLLSGRLEIAVSYGYAVPEELSG
ELLIDLPPLLVVAADHPLAGKSVVSLQDVADEPFLLYDLPHTRDYFFSLFDAAGITPRIAYRSRSYELIRGLVGQKRGYT
LHNASPRTTMAYDGSRVAVRPLKERLPPVKVMRLTLRRQPMRPAVEVFARFLSDAFSPGGMFAPGSIAPDMDARSRDHLA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=32.2709163346614, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790399, Length=240, Percent_Identity=28.75, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693105, Length=224, Percent_Identity=25.8928571428571, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787530, Length=239, Percent_Identity=28.4518828451883, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787601, Length=239, Percent_Identity=26.7782426778243, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=31.7391304347826, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367136, Length=273, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=24.406779661017, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789204, Length=259, Percent_Identity=25.4826254826255, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790208, Length=201, Percent_Identity=28.8557213930348, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788297, Length=183, Percent_Identity=30.6010928961749, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=231, Percent_Identity=24.2424242424242, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=298, Percent_Identity=26.1744966442953, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=196, Percent_Identity=27.0408163265306, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35045; Mature: 35045

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSLRALRYVVAAADCGNVTEAARQLNVSQPSISAAIAQTEADLKLQLFVRHHARGISL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEHHHCCCCEE
TSAGQRYVNEARHLLAHARDFAQTAEALGDSLRGEIALGCFLTIAPRFMPALLARFAEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PGITVKLEEGDQREILDGLLSGRLEIAVSYGYAVPEELSGELLIDLPPLLVVAADHPLAG
CCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCHHCCCCEEEECCCEEEEECCCCCCC
KSVVSLQDVADEPFLLYDLPHTRDYFFSLFDAAGITPRIAYRSRSYELIRGLVGQKRGYT
CCCEEHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCCCE
LHNASPRTTMAYDGSRVAVRPLKERLPPVKVMRLTLRRQPMRPAVEVFARFLSDAFSPGG
EECCCCCCEEEECCCEEEECHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
MFAPGSIAPDMDARSRDHLA
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVSLRALRYVVAAADCGNVTEAARQLNVSQPSISAAIAQTEADLKLQLFVRHHARGISL
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEHHHCCCCEE
TSAGQRYVNEARHLLAHARDFAQTAEALGDSLRGEIALGCFLTIAPRFMPALLARFAEVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PGITVKLEEGDQREILDGLLSGRLEIAVSYGYAVPEELSGELLIDLPPLLVVAADHPLAG
CCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCHHCCCCEEEECCCEEEEECCCCCCC
KSVVSLQDVADEPFLLYDLPHTRDYFFSLFDAAGITPRIAYRSRSYELIRGLVGQKRGYT
CCCEEHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHCCCCCCE
LHNASPRTTMAYDGSRVAVRPLKERLPPVKVMRLTLRRQPMRPAVEVFARFLSDAFSPGG
EECCCCCCEEEECCCEEEECHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
MFAPGSIAPDMDARSRDHLA
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]