Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 154246497

GI number: 154246497

Start: 2849847

End: 2850740

Strand: Reverse

Name: ilvE [H]

Synonym: Xaut_2556

Alternate gene names: 154246497

Gene position: 2850740-2849847 (Counterclockwise)

Preceding gene: 154246504

Following gene: 154246496

Centisome position: 53.7

GC content: 66.0

Gene sequence:

>894_bases
ATGAGCGCGCAGGGTGTCCCCTTCGACCAACGTGACGGCTGGATCTGGTACGACGGCGTGTTCGTGCCGTGGGCCGACGC
CAAGCTGCACGTGCTGTCCCATGGCCTGCACTACGGCAGCACCGTGTTCGAGGGCGAGCGCGCCTACGGCGGCACGATCT
TCAAGAGCGCAGAGCATTCCGAGCGCCTGCGCCTGTCCGCCGAGATGCTGGACTTCACCATCCCCTACACCATCGGCGAG
ATCGACGCGGCCAAGAAGGCGACGCTCGAGAAGAACAATCTCGTGGATGCCTATATCCGCCCCGTCGCCTGGCGTGGCAG
CGAGATGATGGGCGTCGCCGCCCAGAAGAACACCATCCACCTCGCCATCGGCGTGTGGGAATGGCCGAGCTATTTCGATC
CCGAGCAGCGCCAGAAGGGCCTCAGGCTCGACCTGTCGGAATGGCGCCGGCCCGATCCCGCCACCATCCCCTGCAAGGCG
AAGGCTGCCGGTCTCTACATGATCTGCACCATCTCCAAGCACCGGGCCGAGCGCCGCGGCTTCGCCGACGCGCTGATGCT
CGACTATCGCGGCTATGTGGCCGAGTGCACCGGCGCCAACGTCTTCTTTGTGAAGGACGGCGTCATCCACACGCCCACTC
CGGACTGCTTCCTCGACGGCCTTACCCGCCGCACGGTGATCGACATCGCCCGCCGCCACGGCATGGAGGTGGTGGAGCGC
CACATCAAGCCGGAGGAGCTGTCTTCCTTCACCGAATGCTTCATCACCGGCACCGCCGCCGAGGTGACCCCGGTGGGCTC
CATCGCCGACTGGCACTTCGCTCCCTCGGGCATCACCCATCAGCTGATGGATTCCTACACCGCCGAGGTGCGCCCGAAGC
AGCAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCAGGCGTAAAGATATCCCCATGCGGCATCGCTGAAAAGCGGCGGTTGTAGCGGGGAGAAGCCGCGCGGACGACGCGGTT
GGAAAAAGGAGTTCGAGATC

Downstream 100 bases:

>100_bases
CACGGCCTTCGCATTGAAATGAAAAAGGGCGTCGCTTGCGCGGCGCCCTTTTTCTTTGCGCCGGGGCTGGAACGCCCGCA
GCGAAGCCTTCCCGTCACTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSAQGVPFDQRDGWIWYDGVFVPWADAKLHVLSHGLHYGSTVFEGERAYGGTIFKSAEHSERLRLSAEMLDFTIPYTIGE
IDAAKKATLEKNNLVDAYIRPVAWRGSEMMGVAAQKNTIHLAIGVWEWPSYFDPEQRQKGLRLDLSEWRRPDPATIPCKA
KAAGLYMICTISKHRAERRGFADALMLDYRGYVAECTGANVFFVKDGVIHTPTPDCFLDGLTRRTVIDIARRHGMEVVER
HIKPEELSSFTECFITGTAAEVTPVGSIADWHFAPSGITHQLMDSYTAEVRPKQQAA

Sequences:

>Translated_297_residues
MSAQGVPFDQRDGWIWYDGVFVPWADAKLHVLSHGLHYGSTVFEGERAYGGTIFKSAEHSERLRLSAEMLDFTIPYTIGE
IDAAKKATLEKNNLVDAYIRPVAWRGSEMMGVAAQKNTIHLAIGVWEWPSYFDPEQRQKGLRLDLSEWRRPDPATIPCKA
KAAGLYMICTISKHRAERRGFADALMLDYRGYVAECTGANVFFVKDGVIHTPTPDCFLDGLTRRTVIDIARRHGMEVVER
HIKPEELSSFTECFITGTAAEVTPVGSIADWHFAPSGITHQLMDSYTAEVRPKQQAA
>Mature_296_residues
SAQGVPFDQRDGWIWYDGVFVPWADAKLHVLSHGLHYGSTVFEGERAYGGTIFKSAEHSERLRLSAEMLDFTIPYTIGEI
DAAKKATLEKNNLVDAYIRPVAWRGSEMMGVAAQKNTIHLAIGVWEWPSYFDPEQRQKGLRLDLSEWRRPDPATIPCKAK
AAGLYMICTISKHRAERRGFADALMLDYRGYVAECTGANVFFVKDGVIHTPTPDCFLDGLTRRTVIDIARRHGMEVVERH
IKPEELSSFTECFITGTAAEVTPVGSIADWHFAPSGITHQLMDSYTAEVRPKQQAA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=265, Percent_Identity=39.622641509434, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33322; Mature: 33191

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQGVPFDQRDGWIWYDGVFVPWADAKLHVLSHGLHYGSTVFEGERAYGGTIFKSAEHS
CCCCCCCCCCCCCEEEECCEEEECCCCEEEEHHCCCCCCCEEECCCCCCCCEEECCCCHH
ERLRLSAEMLDFTIPYTIGEIDAAKKATLEKNNLVDAYIRPVAWRGSEMMGVAAQKNTIH
HHHHHHHHHHEEECCEEECCCCCHHHHHCCCCCCHHHHHCCEEECCCCEEEEEECCCEEE
LAIGVWEWPSYFDPEQRQKGLRLDLSEWRRPDPATIPCKAKAAGLYMICTISKHRAERRG
EEEEEECCCCCCCHHHHHCCCEECHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCC
FADALMLDYRGYVAECTGANVFFVKDGVIHTPTPDCFLDGLTRRTVIDIARRHGMEVVER
HHHHHHHHCCCCEEEECCCEEEEEECCEEECCCCHHHHHCCHHHHHHHHHHHCCHHHHHH
HIKPEELSSFTECFITGTAAEVTPVGSIADWHFAPSGITHQLMDSYTAEVRPKQQAA
CCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SAQGVPFDQRDGWIWYDGVFVPWADAKLHVLSHGLHYGSTVFEGERAYGGTIFKSAEHS
CCCCCCCCCCCCEEEECCEEEECCCCEEEEHHCCCCCCCEEECCCCCCCCEEECCCCHH
ERLRLSAEMLDFTIPYTIGEIDAAKKATLEKNNLVDAYIRPVAWRGSEMMGVAAQKNTIH
HHHHHHHHHHEEECCEEECCCCCHHHHHCCCCCCHHHHHCCEEECCCCEEEEEECCCEEE
LAIGVWEWPSYFDPEQRQKGLRLDLSEWRRPDPATIPCKAKAAGLYMICTISKHRAERRG
EEEEEECCCCCCCHHHHHCCCEECHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCC
FADALMLDYRGYVAECTGANVFFVKDGVIHTPTPDCFLDGLTRRTVIDIARRHGMEVVER
HHHHHHHHCCCCEEEECCCEEEEEECCEEECCCCHHHHHCCHHHHHHHHHHHCCHHHHHH
HIKPEELSSFTECFITGTAAEVTPVGSIADWHFAPSGITHQLMDSYTAEVRPKQQAA
CCCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]