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The map label for this gene is citA [H]

Identifier: 154246215

GI number: 154246215

Start: 2531614

End: 2532936

Strand: Direct

Name: citA [H]

Synonym: Xaut_2273

Alternate gene names: 154246215

Gene position: 2531614-2532936 (Clockwise)

Preceding gene: 154246214

Following gene: 154246216

Centisome position: 47.69

GC content: 63.72

Gene sequence:

>1323_bases
ATGGTCGGGCCTTCGGCAACCGCCGTTCCATCCACCGTGACCGCAGAGGTGCCTGCGATCAGATCGAGAACCGCAGCAAT
CCTGCGCTCGACCAGCGGAAACTTTCTTGAGCAGTTCGACTTTTTTCTTTTTGGTTTCTACGCGACGTCGATTTCCAAGG
CGTTCTTCCCGACCGGAAATGAAGTCACGGAACTGCTCCTCACCTTCACGACCTTCTGGCTCGGCGCGCTGATGCGGCCG
GTGGGCGCGATCGTGCTCGGCTCCTATCTCGATCATATTGGCCGCCGCAAGGGACTGATCGTGACGCTCGGCATCATGGC
CGCCGGCACGGTGCTCATCGCAGTGTGCCCCACCTACGCACAGATCGGCATCGCCGCGCCGCTCATCGTGCTCTTCGGTC
GCCTGTTGCAGGGCTTCTCGGCGGGCGTGGAGCTGGGCGGCGTCTCGATCTACCTGTTCGAGATCGCGACGCCCGGCAAC
AAGGGCTTCTACACCTCGTTCCAGTCGGCCAGCCAGCAGGTTGCCATCTTCTTTGCGGCCATCATCGGCTTCTCGCTGAG
CCAGCTGATGCCGTCCGAGACCATCTCCGCGTGGGGCTGGCGCATTCCCTTCTTCATCGGCTGCGCGATCATCCCCTTCA
TCTTCTTCATCCGCCGGACGCTTGAGGAGACCCCCGAGTTCCTCAAGCAGAAGAAGCACCCGACCCAGAGCGAAGTCTTC
GCCGCGATGCTCACCAACTGGCGGATCGTGCTGCTCGGCATGATGCTGACCGCCATGACCACGGTGACCTTCTACTTCAT
CACCGTGTACACCCCGACCTTCGGCAAGAACGTGCTGAAGCTGTCCATCACCGACAGTCTTCTGGTCACGCTGCTGGTAG
CGACAACCAACTTCATCTGGCTGCCCATCGGCGGCGCGATCTCGGACCGCGTGGGTCGCCGTCCGGTGCTGCTGACCATC
GCCATCCTGGCGCTGGCGACGGCCTATCCCGCCCTCGCGTGGCTGGTGGTGGAGCCGACCTTCAACAAGATGCTGATGGT
GGAGCTGTGGTTCTCGCTGTGCTTCGGCGTCTATAACGGCGCCATGGTGGCGGCCTTGTCCGAAGAGGTGCCCGCCCATG
TGCGCACCACCTGCTTCTCGCTCGCCTTCGCCCTCGCCGCAGCCCTGTTCGGCACGTTCACGCCCCTGGTCTCCACCTGG
CTGATCGACATGACGGGGAACCGCGCGGCACCGGGCTTCTGGCTCATGGCCGCGGCCGCCTCGGGCCTGATCGCGACCAT
CCTCATCTATCGCGGACGGGGCGCGGCCCCCGCGCGGAGTTGA

Upstream 100 bases:

>100_bases
AGTCTCTGCAAGGGACCGGCGGGCTGCAGGCGGCTGGACGAAGCAACAACAAGCAGCGCAGGGACGCCGCGTGCGGCTGC
GCCGGCATGGGAGACAACGC

Downstream 100 bases:

>100_bases
CGTGATGGACGCGACACTCGCGCGCGTGGACGTCGTCGTCATCGGTGGCGGCAACGCCGGGCTTTGCGCTGCCATCTCAG
CGCGACGCTCCGGCGCTTCC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MVGPSATAVPSTVTAEVPAIRSRTAAILRSTSGNFLEQFDFFLFGFYATSISKAFFPTGNEVTELLLTFTTFWLGALMRP
VGAIVLGSYLDHIGRRKGLIVTLGIMAAGTVLIAVCPTYAQIGIAAPLIVLFGRLLQGFSAGVELGGVSIYLFEIATPGN
KGFYTSFQSASQQVAIFFAAIIGFSLSQLMPSETISAWGWRIPFFIGCAIIPFIFFIRRTLEETPEFLKQKKHPTQSEVF
AAMLTNWRIVLLGMMLTAMTTVTFYFITVYTPTFGKNVLKLSITDSLLVTLLVATTNFIWLPIGGAISDRVGRRPVLLTI
AILALATAYPALAWLVVEPTFNKMLMVELWFSLCFGVYNGAMVAALSEEVPAHVRTTCFSLAFALAAALFGTFTPLVSTW
LIDMTGNRAAPGFWLMAAAASGLIATILIYRGRGAAPARS

Sequences:

>Translated_440_residues
MVGPSATAVPSTVTAEVPAIRSRTAAILRSTSGNFLEQFDFFLFGFYATSISKAFFPTGNEVTELLLTFTTFWLGALMRP
VGAIVLGSYLDHIGRRKGLIVTLGIMAAGTVLIAVCPTYAQIGIAAPLIVLFGRLLQGFSAGVELGGVSIYLFEIATPGN
KGFYTSFQSASQQVAIFFAAIIGFSLSQLMPSETISAWGWRIPFFIGCAIIPFIFFIRRTLEETPEFLKQKKHPTQSEVF
AAMLTNWRIVLLGMMLTAMTTVTFYFITVYTPTFGKNVLKLSITDSLLVTLLVATTNFIWLPIGGAISDRVGRRPVLLTI
AILALATAYPALAWLVVEPTFNKMLMVELWFSLCFGVYNGAMVAALSEEVPAHVRTTCFSLAFALAAALFGTFTPLVSTW
LIDMTGNRAAPGFWLMAAAASGLIATILIYRGRGAAPARS
>Mature_440_residues
MVGPSATAVPSTVTAEVPAIRSRTAAILRSTSGNFLEQFDFFLFGFYATSISKAFFPTGNEVTELLLTFTTFWLGALMRP
VGAIVLGSYLDHIGRRKGLIVTLGIMAAGTVLIAVCPTYAQIGIAAPLIVLFGRLLQGFSAGVELGGVSIYLFEIATPGN
KGFYTSFQSASQQVAIFFAAIIGFSLSQLMPSETISAWGWRIPFFIGCAIIPFIFFIRRTLEETPEFLKQKKHPTQSEVF
AAMLTNWRIVLLGMMLTAMTTVTFYFITVYTPTFGKNVLKLSITDSLLVTLLVATTNFIWLPIGGAISDRVGRRPVLLTI
AILALATAYPALAWLVVEPTFNKMLMVELWFSLCFGVYNGAMVAALSEEVPAHVRTTCFSLAFALAAALFGTFTPLVSTW
LIDMTGNRAAPGFWLMAAAASGLIATILIYRGRGAAPARS

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=30.4147465437788, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788942, Length=447, Percent_Identity=32.2147651006711, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788292, Length=405, Percent_Identity=26.6666666666667, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=30.5936073059361, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87082231, Length=267, Percent_Identity=27.7153558052434, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47602; Mature: 47602

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGPSATAVPSTVTAEVPAIRSRTAAILRSTSGNFLEQFDFFLFGFYATSISKAFFPTGN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVTELLLTFTTFWLGALMRPVGAIVLGSYLDHIGRRKGLIVTLGIMAAGTVLIAVCPTYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHH
QIGIAAPLIVLFGRLLQGFSAGVELGGVSIYLFEIATPGNKGFYTSFQSASQQVAIFFAA
HHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
IIGFSLSQLMPSETISAWGWRIPFFIGCAIIPFIFFIRRTLEETPEFLKQKKHPTQSEVF
HHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAMLTNWRIVLLGMMLTAMTTVTFYFITVYTPTFGKNVLKLSITDSLLVTLLVATTNFIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHCCEEE
LPIGGAISDRVGRRPVLLTIAILALATAYPALAWLVVEPTFNKMLMVELWFSLCFGVYNG
EECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCC
AMVAALSEEVPAHVRTTCFSLAFALAAALFGTFTPLVSTWLIDMTGNRAAPGFWLMAAAA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
SGLIATILIYRGRGAAPARS
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVGPSATAVPSTVTAEVPAIRSRTAAILRSTSGNFLEQFDFFLFGFYATSISKAFFPTGN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVTELLLTFTTFWLGALMRPVGAIVLGSYLDHIGRRKGLIVTLGIMAAGTVLIAVCPTYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCHHH
QIGIAAPLIVLFGRLLQGFSAGVELGGVSIYLFEIATPGNKGFYTSFQSASQQVAIFFAA
HHHHHHHHHHHHHHHHHHHHCCCEECCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
IIGFSLSQLMPSETISAWGWRIPFFIGCAIIPFIFFIRRTLEETPEFLKQKKHPTQSEVF
HHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAMLTNWRIVLLGMMLTAMTTVTFYFITVYTPTFGKNVLKLSITDSLLVTLLVATTNFIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHHHHHHCCEEE
LPIGGAISDRVGRRPVLLTIAILALATAYPALAWLVVEPTFNKMLMVELWFSLCFGVYNG
EECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCC
AMVAALSEEVPAHVRTTCFSLAFALAAALFGTFTPLVSTWLIDMTGNRAAPGFWLMAAAA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
SGLIATILIYRGRGAAPARS
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]