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The map label for this gene is cueO [H]

Identifier: 154246212

GI number: 154246212

Start: 2526831

End: 2528339

Strand: Reverse

Name: cueO [H]

Synonym: Xaut_2270

Alternate gene names: 154246212

Gene position: 2528339-2526831 (Counterclockwise)

Preceding gene: 154246213

Following gene: 154246211

Centisome position: 47.62

GC content: 67.13

Gene sequence:

>1509_bases
GTGAGCATATTGAACCCACCACTGCACAATCTTGACAGGCGACAGGTCTTGCTGGGCGGCGCGGCGCTGCTGGCGGCTCC
GTTCGTGCTGACCGGGCGCGCCTTCGCGGCGGAAGGCGGCGCGGCGTTGCCGATCCCGCCGATCCTAGAGGCCGACGGCG
CGCAGCCGGTCACGCTGACCGCGCGAAAAGGCAGCCACGCCTTCGTGCCCGGCACGCAGACGCCGACGCTAGGGTATGAG
GCCGATTACCTCGGGCCGACGCTGCGAGTGCGGCGCGGGCGCACCGCGCAGATCGACGTTGTCAACAGGACCGACGACGT
GGTCACGGCGCATTGGCACGGGGCCCATGTCCCGGGCAACATGGATGGCGGCCCGCAGACGGCCTTCGCCCCCGGCCAGA
CGTGGTCGGCGCGGTTCGATGTGCATCACCCGGCGGCGACGCTGTGGTATCACAGCCATGTCCACGGCAAGACCGGGCCG
CAGGTCTACCACGGGCTCGCGGGCCTGTTGTTGGTCGACGATCCCGCCGCGCCCGACGGCCTGCCTTCGGACTATGGTGT
GAACGATATCCCGCTGGTGGTGCAGGACCGCAGCTTCGACAGCTCCGGGCGGCTGGTCTATGACATTTCCGGCATGATGT
CCCTGATGGGCGGTTTTCTGGGCGACACGATCTGCGTCAACGGTGCGGTCCGACCGGTCGCGGATGTGCCGCGCGGACTG
GTGCGGCTCCGCCTGCTCAATGGCTCGAACGCGCGGTTTTATGATTTTACCTTTGCCGACGGGCGGCAGTTCCATCAGGT
CGCAAGCGACGGCGGGTTGCTGCCCGCGCCGGTCGCGCGCAATCGCATCGCCCTGGCCCCGGCCGAGCGCGCCGAGATCG
TCGTCGATTTCAGCGCGGACAGCGCGCCTGTCCGCCTGATCAGCAGCGACCTCGCGCAGGGTGGCATGATGGGGGGCGGC
ATGATGGGCGGTGGGGCCGGTTCCGGCGGAACGCTGGATATCCTGACCTTTTCGCCGGGATCAGCCCAGGGCAATGCTCC
CTCCGCACTGCCCGCCGCGCTCCCCGCACCGCCATTCGATCTCGGCGCCCCAGTGCGCACCCGCGAATTCCGGCTCGATA
TGCACGCGGGGTCCATGATTGGGTCGATGGCCAGAAGGCTATTCGGCGCCACCGGCAGTATCATGGGCATCAACGGCAAC
GCCTACGACATGGGCCGCATCGACGCGGCCCTGACCCTCGGCGAGACTGAGTTGTGGCGGGTGAGCGCGCCCATGATGGT
GCATCCGTTCCACGTGCATGGGTGTTCGTTCCAAGTGGTGTCGCGCGGGGGCCAGCCCGTCGACCCCGCGACAGAGGGAC
ACAAGGATGTGGTGGTCGTCGACGCAAGCGGCGTCGAAATCCTGGTCCAGGTCAACAAAAAGGCGGATGCGGCAGATCCG
TTCATGCTGCATTGTCACATCCTTGAACATGAGGATGCCGGCATGATGGCGCAATTCACCGTGACCTGA

Upstream 100 bases:

>100_bases
CCTCAAACGACACGCCACTTGGGGAATGCAGGCTGATGCGAATATATGTCCGACAAGAGATGCCAAACCACCCTGCAAAC
ACAAGACAAGGGTCTCGATC

Downstream 100 bases:

>100_bases
CCTGCGAGGGGCAAATGCAGAATAAATTCTCCGGGGCCCTTTGATCCTCCAGTTACTGGAACCCCTATGTTCGAAAATAT
CGAAGCCGCGGTTGACAGGA

Product: Bilirubin oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSILNPPLHNLDRRQVLLGGAALLAAPFVLTGRAFAAEGGAALPIPPILEADGAQPVTLTARKGSHAFVPGTQTPTLGYE
ADYLGPTLRVRRGRTAQIDVVNRTDDVVTAHWHGAHVPGNMDGGPQTAFAPGQTWSARFDVHHPAATLWYHSHVHGKTGP
QVYHGLAGLLLVDDPAAPDGLPSDYGVNDIPLVVQDRSFDSSGRLVYDISGMMSLMGGFLGDTICVNGAVRPVADVPRGL
VRLRLLNGSNARFYDFTFADGRQFHQVASDGGLLPAPVARNRIALAPAERAEIVVDFSADSAPVRLISSDLAQGGMMGGG
MMGGGAGSGGTLDILTFSPGSAQGNAPSALPAALPAPPFDLGAPVRTREFRLDMHAGSMIGSMARRLFGATGSIMGINGN
AYDMGRIDAALTLGETELWRVSAPMMVHPFHVHGCSFQVVSRGGQPVDPATEGHKDVVVVDASGVEILVQVNKKADAADP
FMLHCHILEHEDAGMMAQFTVT

Sequences:

>Translated_502_residues
MSILNPPLHNLDRRQVLLGGAALLAAPFVLTGRAFAAEGGAALPIPPILEADGAQPVTLTARKGSHAFVPGTQTPTLGYE
ADYLGPTLRVRRGRTAQIDVVNRTDDVVTAHWHGAHVPGNMDGGPQTAFAPGQTWSARFDVHHPAATLWYHSHVHGKTGP
QVYHGLAGLLLVDDPAAPDGLPSDYGVNDIPLVVQDRSFDSSGRLVYDISGMMSLMGGFLGDTICVNGAVRPVADVPRGL
VRLRLLNGSNARFYDFTFADGRQFHQVASDGGLLPAPVARNRIALAPAERAEIVVDFSADSAPVRLISSDLAQGGMMGGG
MMGGGAGSGGTLDILTFSPGSAQGNAPSALPAALPAPPFDLGAPVRTREFRLDMHAGSMIGSMARRLFGATGSIMGINGN
AYDMGRIDAALTLGETELWRVSAPMMVHPFHVHGCSFQVVSRGGQPVDPATEGHKDVVVVDASGVEILVQVNKKADAADP
FMLHCHILEHEDAGMMAQFTVT
>Mature_501_residues
SILNPPLHNLDRRQVLLGGAALLAAPFVLTGRAFAAEGGAALPIPPILEADGAQPVTLTARKGSHAFVPGTQTPTLGYEA
DYLGPTLRVRRGRTAQIDVVNRTDDVVTAHWHGAHVPGNMDGGPQTAFAPGQTWSARFDVHHPAATLWYHSHVHGKTGPQ
VYHGLAGLLLVDDPAAPDGLPSDYGVNDIPLVVQDRSFDSSGRLVYDISGMMSLMGGFLGDTICVNGAVRPVADVPRGLV
RLRLLNGSNARFYDFTFADGRQFHQVASDGGLLPAPVARNRIALAPAERAEIVVDFSADSAPVRLISSDLAQGGMMGGGM
MGGGAGSGGTLDILTFSPGSAQGNAPSALPAALPAPPFDLGAPVRTREFRLDMHAGSMIGSMARRLFGATGSIMGINGNA
YDMGRIDAALTLGETELWRVSAPMMVHPFHVHGCSFQVVSRGGQPVDPATEGHKDVVVVDASGVEILVQVNKKADAADPF
MLHCHILEHEDAGMMAQFTVT

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=527, Percent_Identity=37.0018975332068, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1789394, Length=495, Percent_Identity=35.7575757575758, Blast_Score=271, Evalue=8e-74,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 52675; Mature: 52543

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILNPPLHNLDRRQVLLGGAALLAAPFVLTGRAFAAEGGAALPIPPILEADGAQPVTLT
CCCCCCCCCCCCCEEEEECCHHHHHCCHHEECCEEECCCCCCCCCCCCCCCCCCCCEEEE
ARKGSHAFVPGTQTPTLGYEADYLGPTLRVRRGRTAQIDVVNRTDDVVTAHWHGAHVPGN
EECCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCC
MDGGPQTAFAPGQTWSARFDVHHPAATLWYHSHVHGKTGPQVYHGLAGLLLVDDPAAPDG
CCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHCCEEEEEEECCCCCCC
LPSDYGVNDIPLVVQDRSFDSSGRLVYDISGMMSLMGGFLGDTICVNGAVRPVADVPRGL
CCCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCE
VRLRLLNGSNARFYDFTFADGRQFHQVASDGGLLPAPVARNRIALAPAERAEIVVDFSAD
EEEEEECCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEEECCC
SAPVRLISSDLAQGGMMGGGMMGGGAGSGGTLDILTFSPGSAQGNAPSALPAALPAPPFD
CCCHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
LGAPVRTREFRLDMHAGSMIGSMARRLFGATGSIMGINGNAYDMGRIDAALTLGETELWR
CCCCCEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCEEECCCEEEEEEECCCEEEE
VSAPMMVHPFHVHGCSFQVVSRGGQPVDPATEGHKDVVVVDASGVEILVQVNKKADAADP
ECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCC
FMLHCHILEHEDAGMMAQFTVT
EEEEEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SILNPPLHNLDRRQVLLGGAALLAAPFVLTGRAFAAEGGAALPIPPILEADGAQPVTLT
CCCCCCCCCCCCEEEEECCHHHHHCCHHEECCEEECCCCCCCCCCCCCCCCCCCCEEEE
ARKGSHAFVPGTQTPTLGYEADYLGPTLRVRRGRTAQIDVVNRTDDVVTAHWHGAHVPGN
EECCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCC
MDGGPQTAFAPGQTWSARFDVHHPAATLWYHSHVHGKTGPQVYHGLAGLLLVDDPAAPDG
CCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHCCEEEEEEECCCCCCC
LPSDYGVNDIPLVVQDRSFDSSGRLVYDISGMMSLMGGFLGDTICVNGAVRPVADVPRGL
CCCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCE
VRLRLLNGSNARFYDFTFADGRQFHQVASDGGLLPAPVARNRIALAPAERAEIVVDFSAD
EEEEEECCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEEECCC
SAPVRLISSDLAQGGMMGGGMMGGGAGSGGTLDILTFSPGSAQGNAPSALPAALPAPPFD
CCCHHHHHHHHHCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
LGAPVRTREFRLDMHAGSMIGSMARRLFGATGSIMGINGNAYDMGRIDAALTLGETELWR
CCCCCEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCEEECCCEEEEEEECCCEEEE
VSAPMMVHPFHVHGCSFQVVSRGGQPVDPATEGHKDVVVVDASGVEILVQVNKKADAADP
ECCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCC
FMLHCHILEHEDAGMMAQFTVT
EEEEEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]