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The map label for this gene is sppA [C]

Identifier: 154246163

GI number: 154246163

Start: 2480653

End: 2481624

Strand: Reverse

Name: sppA [C]

Synonym: Xaut_2220

Alternate gene names: 154246163

Gene position: 2481624-2480653 (Counterclockwise)

Preceding gene: 154246164

Following gene: 154246162

Centisome position: 46.74

GC content: 69.75

Gene sequence:

>972_bases
ATGTCGCTCGACGCGGACCAGATCGTTGAACGCCGCCGCCTGCGGCGGAAGGTCACTTTCTGGCGCACCCTCGGTGTGCT
CGCCGCTGTCGCCGCCCTTGCGGTGGGTGTGGCCTCCGTGGCGGGCGACGGCGTTTCGGCCTCCTCGCCCCATGTGGCGC
GGGTGACCATCGGCGGCATCATCCGCAACGACCGCGCGCGGGTCGAACTCTTGGAGGAGATCGGCCGCTCGCGGGCGCGG
GCGGTGATCTTGTCCATCGACAGCCCCGGCGGCACCGTCACCGGCTCCGAGCAATTGTTCGACGCTTTGCGTCGCCTTTC
CGAAAAGAAGCCGGTGGTCGCCGTGGTGGAAGGCATCGCCGCATCCGGGGCCTATATCGCGGCGCTGGGTGCCGACCACA
TCGTGGCCCGGCGCAACGCGCTGGTGGGCTCGGTGGGCGTGATCTACCAGTATCCCAATGTCACCGAGCTGCTGAAGACC
GTGGGCGTCGCCATGGAGGACATCAAGTCCTCGCCGCTCAAGGCCTCGCCCAATCCCTACACCCCCACCACGCCGGAAGC
GCGGGCGGCGGTGGAGTCGCTGGTGAAGGACAGCTACGCATGGTTCAAGGGCCTGGTGAGCGAACGGCGCAAGATGTCCG
ACGACAAGCTCGCCACCGTCACCGACGGGCGCGTCTTCACCGGCCATCAGGGCCTTGACCTGCAGCTGGTGGACGAGTTG
GGCGACGAGCGCACCGCCCGGGCATGGCTCGCCCGCGAGAAGGGCGTTCCGGAAAACCTCAAGGTCCGCAGCTGGCGCAC
CCGGGAGGTGGATTCGGAGTTCGGCTGGCTGCTCGGCGCGGCGCGGGGCGCGGTGGCGGCGCTGGGCTTCCCCCAGGCGG
CCGAGTTCCTCACCCAGACGGCCCGGGGGGCGCTGGAACGCGCCCAGCTTGACGGTCTTCTGGCCCTCTGGCACCCTCGC
ACCGACGGGTGA

Upstream 100 bases:

>100_bases
TTGACCACGAGTGCCTTGCCAACCCCGGGCCACCTGCTTAAGTGCGGCCCATCGGTCCTGTCCGCCTCCGGCGGCCGGAG
TGCCCTGAAGGAGCTGAGTC

Downstream 100 bases:

>100_bases
CAAGGGCTTGAGGCTCCTGCATTAAACGCGGCAATCCGTCGCCGGAAAGCAGCCTGTCCGCCAAACCCAGCGGATCGTTC
TCAAGGGACATGCGGCCATC

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSLDADQIVERRRLRRKVTFWRTLGVLAAVAALAVGVASVAGDGVSASSPHVARVTIGGIIRNDRARVELLEEIGRSRAR
AVILSIDSPGGTVTGSEQLFDALRRLSEKKPVVAVVEGIAASGAYIAALGADHIVARRNALVGSVGVIYQYPNVTELLKT
VGVAMEDIKSSPLKASPNPYTPTTPEARAAVESLVKDSYAWFKGLVSERRKMSDDKLATVTDGRVFTGHQGLDLQLVDEL
GDERTARAWLAREKGVPENLKVRSWRTREVDSEFGWLLGAARGAVAALGFPQAAEFLTQTARGALERAQLDGLLALWHPR
TDG

Sequences:

>Translated_323_residues
MSLDADQIVERRRLRRKVTFWRTLGVLAAVAALAVGVASVAGDGVSASSPHVARVTIGGIIRNDRARVELLEEIGRSRAR
AVILSIDSPGGTVTGSEQLFDALRRLSEKKPVVAVVEGIAASGAYIAALGADHIVARRNALVGSVGVIYQYPNVTELLKT
VGVAMEDIKSSPLKASPNPYTPTTPEARAAVESLVKDSYAWFKGLVSERRKMSDDKLATVTDGRVFTGHQGLDLQLVDEL
GDERTARAWLAREKGVPENLKVRSWRTREVDSEFGWLLGAARGAVAALGFPQAAEFLTQTARGALERAQLDGLLALWHPR
TDG
>Mature_322_residues
SLDADQIVERRRLRRKVTFWRTLGVLAAVAALAVGVASVAGDGVSASSPHVARVTIGGIIRNDRARVELLEEIGRSRARA
VILSIDSPGGTVTGSEQLFDALRRLSEKKPVVAVVEGIAASGAYIAALGADHIVARRNALVGSVGVIYQYPNVTELLKTV
GVAMEDIKSSPLKASPNPYTPTTPEARAAVESLVKDSYAWFKGLVSERRKMSDDKLATVTDGRVFTGHQGLDLQLVDELG
DERTARAWLAREKGVPENLKVRSWRTREVDSEFGWLLGAARGAVAALGFPQAAEFLTQTARGALERAQLDGLLALWHPRT
DG

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=190, Percent_Identity=32.6315789473684, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787527, Length=161, Percent_Identity=32.9192546583851, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 34725; Mature: 34594

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDADQIVERRRLRRKVTFWRTLGVLAAVAALAVGVASVAGDGVSASSPHVARVTIGGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCH
IRNDRARVELLEEIGRSRARAVILSIDSPGGTVTGSEQLFDALRRLSEKKPVVAVVEGIA
HCCCHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
ASGAYIAALGADHIVARRNALVGSVGVIYQYPNVTELLKTVGVAMEDIKSSPLKASPNPY
CCCCEEEECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TPTTPEARAAVESLVKDSYAWFKGLVSERRKMSDDKLATVTDGRVFTGHQGLDLQLVDEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCHHHHHC
GDERTARAWLAREKGVPENLKVRSWRTREVDSEFGWLLGAARGAVAALGFPQAAEFLTQT
CCHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARGALERAQLDGLLALWHPRTDG
HHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure 
SLDADQIVERRRLRRKVTFWRTLGVLAAVAALAVGVASVAGDGVSASSPHVARVTIGGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCH
IRNDRARVELLEEIGRSRARAVILSIDSPGGTVTGSEQLFDALRRLSEKKPVVAVVEGIA
HCCCHHHHHHHHHHCHHHHEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
ASGAYIAALGADHIVARRNALVGSVGVIYQYPNVTELLKTVGVAMEDIKSSPLKASPNPY
CCCCEEEECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TPTTPEARAAVESLVKDSYAWFKGLVSERRKMSDDKLATVTDGRVFTGHQGLDLQLVDEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCHHHHHC
GDERTARAWLAREKGVPENLKVRSWRTREVDSEFGWLLGAARGAVAALGFPQAAEFLTQT
CCHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ARGALERAQLDGLLALWHPRTDG
HHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]