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The map label for this gene is gcvH

Identifier: 154246143

GI number: 154246143

Start: 2459499

End: 2459876

Strand: Reverse

Name: gcvH

Synonym: Xaut_2200

Alternate gene names: 154246143

Gene position: 2459876-2459499 (Counterclockwise)

Preceding gene: 154246144

Following gene: 154246142

Centisome position: 46.33

GC content: 64.81

Gene sequence:

>378_bases
ATGACGAGCGTGCGCTACACGAAGGACCACGAATATGTGCGCGTCGAAGGCGACGTGGCCGTGGTGGGCATCTCCGACTA
TGCCCAGCAGCAGCTGGGTGACGTGGTGTTCGTCGAGCTGCCGGAGATCGGCAAGGTCGTGACCAAGGGCGGCGAGGCCG
CCGTGGTGGAGAGCGTGAAGGCGGCGAGCGAGGTCTACGCCCCGCTCTCCGGCGAGGTGGTGGAGGTGAACAGCGAGCTG
GAAGGCGCCCCCGGCCTCGTCAACGAGGCTCCGGAAGGCAAGGGCTGGTTCATGAAGCTGAAGCTCTCCAACCCCGCCGA
GCTGGACGAGCTTTTGAATGAGCACGCCTACAAGGACTTCCTGGACACGTTGGCCTGA

Upstream 100 bases:

>100_bases
CGACGTTCTGGTGCGCGGCAAGGCTTTGGCGGCCACTGTCGCATCCCTCCCGTTCGTGCCGACGCGCTATGCGCGCAAAC
CTAAAACTGGGGCCTGATCC

Downstream 100 bases:

>100_bases
CCATGGCAAGCGACCACAAGGCCACCATCCGCTGGGCGCGGGGCGACGCCGTCTTCACCGATGGGCGCTACGCCCGCGCC
CATGTCTGGGCGTTCGACGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MTSVRYTKDHEYVRVEGDVAVVGISDYAQQQLGDVVFVELPEIGKVVTKGGEAAVVESVKAASEVYAPLSGEVVEVNSEL
EGAPGLVNEAPEGKGWFMKLKLSNPAELDELLNEHAYKDFLDTLA

Sequences:

>Translated_125_residues
MTSVRYTKDHEYVRVEGDVAVVGISDYAQQQLGDVVFVELPEIGKVVTKGGEAAVVESVKAASEVYAPLSGEVVEVNSEL
EGAPGLVNEAPEGKGWFMKLKLSNPAELDELLNEHAYKDFLDTLA
>Mature_124_residues
TSVRYTKDHEYVRVEGDVAVVGISDYAQQQLGDVVFVELPEIGKVVTKGGEAAVVESVKAASEVYAPLSGEVVEVNSELE
GAPGLVNEAPEGKGWFMKLKLSNPAELDELLNEHAYKDFLDTLA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=125, Percent_Identity=50.4, Blast_Score=132, Evalue=7e-32,
Organism=Homo sapiens, GI49574537, Length=125, Percent_Identity=50.4, Blast_Score=132, Evalue=9e-32,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=50, Blast_Score=124, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=50.4347826086956, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=53.9130434782609, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=101, Percent_Identity=50.4950495049505, Blast_Score=107, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=53.3333333333333, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_XANP2 (A7IHF1)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417101.1
- ProteinModelPortal:   A7IHF1
- SMR:   A7IHF1
- STRING:   A7IHF1
- GeneID:   5424379
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2200
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   XAUT78245:XAUT_2200-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13496; Mature: 13365

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVRYTKDHEYVRVEGDVAVVGISDYAQQQLGDVVFVELPEIGKVVTKGGEAAVVESVK
CCCCCCCCCCCEEEEECCEEEEECHHHHHHHHCCEEEEECCCHHHHHHCCCCHHHHHHHH
AASEVYAPLSGEVVEVNSELEGAPGLVNEAPEGKGWFMKLKLSNPAELDELLNEHAYKDF
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLA
HHHCC
>Mature Secondary Structure 
TSVRYTKDHEYVRVEGDVAVVGISDYAQQQLGDVVFVELPEIGKVVTKGGEAAVVESVK
CCCCCCCCCCEEEEECCEEEEECHHHHHHHHCCEEEEECCCHHHHHHCCCCHHHHHHHH
AASEVYAPLSGEVVEVNSELEGAPGLVNEAPEGKGWFMKLKLSNPAELDELLNEHAYKDF
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA