Definition Xanthobacter autotrophicus Py2 chromosome, complete genome.
Accession NC_009720
Length 5,308,934

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The map label for this gene is hoxK [H]

Identifier: 154246116

GI number: 154246116

Start: 2433685

End: 2434797

Strand: Direct

Name: hoxK [H]

Synonym: Xaut_2173

Alternate gene names: 154246116

Gene position: 2433685-2434797 (Clockwise)

Preceding gene: 154246115

Following gene: 154246117

Centisome position: 45.84

GC content: 65.86

Gene sequence:

>1113_bases
ATGGCCCTCGAGACGTTCTATGAAGTCATGCGGCGGCAGGGCATCACCCGCCGCTCGTTCCTGAAATACTGCTCGCTCAC
CGCCGCGGCCCTCGGCCTCGGGCCGGAGTTCGCGCCGCAGATCGCCGAGGCCATGGAGACCAAGCCGCGCACCCCGGTGC
TGTGGCTCCACGGGCTGGAATGCACCTGCTGCTCGGAAAGCTTCATCCGCTCGGCCCATCCCTTGGCCAAGGATGTGGTG
CTGAGCATGATCTCGCTGGATTATGACGACACCCTCATGGCCTCCGCCGGCCACCAGGCGGAAGCGATCCTCGACGAGGT
GATGGAGAAGTACAAGGGCAACTACATCCTCGCGGTGGAAGGCAATCCGCCGCTGAACGAGGATGGCATGTATTGCATCA
TCGGCGGCAAGCCCTTCGTGGACCAGCTGAAGAAGGTGGCGAAGGACGCGAAAGCCATCATCTCCTGGGGCTCCTGCGCC
TCCTATGGCTGCGTGCAGGCGGCGCGCCCCAACCCCACCCGCGCCACTCCGGTGCATGAGGTGATCTTCGATAAGCCCAT
CATCAAGGTGCCGGGCTGCCCGCCCATCGCCGAGGTGATGACCGGCGTCATCACCTACATGCTCACCTTCGACCGCCTGC
CCGACCTCGACCGGCAGGGGCGGCCCAAGATGTTCTATTCCCAGCGCATCCACGACAAGTGCTACCGCCGTCCCCACTTC
GACGCCGGCCAGTATGTGGAGAGCTTCGACGACGAGGGCGCGCGCAAGGGCTACTGCCTCTACAAGGTGGGCTGCAAGGG
TCCCACCACCTACAACGCCTGCTCCACCGTGCGCTGGAACGACGGCGTGTCCTTCCCCATCCAGGCCGGCCACGGCTGCA
TCGGCTGCTCGGAAGACGGCTTCTGGGACAAGGGCTCCTGGTACGCGCGCCTGTCCGACCTGAAGAACGCCGGCTTCGGC
ATTGAATCCAACGCCGACCAGGTGGGCCTCATCGCCGCCGGCGCGGTGGGCGCGGGGGTCGCGGTTCACGCGGCGGTGAG
TGCGCTCAAGCGCGCCCAGCACAAGAATACGGACAACGGCGCTGACACGGGCGCGACGGGAGGCGAGAAGTGA

Upstream 100 bases:

>100_bases
GAAATCCCTCGCACTCCCCCTCTGGCACACCCCTTGCTGAACCCCTCCCAATCCCCCGGCCTCGACGCCGGTGGGGTGGT
CAGCCAAGGGGGAGCGCCGG

Downstream 100 bases:

>100_bases
CCATCCAGACGCCGAATGGCTTTTCCCTCGACACCTCCGGCAAGCGCATCGTGGTCGATCCGGTCACCCGCATCGAAGGC
CACATGCGCTGCGAGGTGAA

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MALETFYEVMRRQGITRRSFLKYCSLTAAALGLGPEFAPQIAEAMETKPRTPVLWLHGLECTCCSESFIRSAHPLAKDVV
LSMISLDYDDTLMASAGHQAEAILDEVMEKYKGNYILAVEGNPPLNEDGMYCIIGGKPFVDQLKKVAKDAKAIISWGSCA
SYGCVQAARPNPTRATPVHEVIFDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPDLDRQGRPKMFYSQRIHDKCYRRPHF
DAGQYVESFDDEGARKGYCLYKVGCKGPTTYNACSTVRWNDGVSFPIQAGHGCIGCSEDGFWDKGSWYARLSDLKNAGFG
IESNADQVGLIAAGAVGAGVAVHAAVSALKRAQHKNTDNGADTGATGGEK

Sequences:

>Translated_370_residues
MALETFYEVMRRQGITRRSFLKYCSLTAAALGLGPEFAPQIAEAMETKPRTPVLWLHGLECTCCSESFIRSAHPLAKDVV
LSMISLDYDDTLMASAGHQAEAILDEVMEKYKGNYILAVEGNPPLNEDGMYCIIGGKPFVDQLKKVAKDAKAIISWGSCA
SYGCVQAARPNPTRATPVHEVIFDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPDLDRQGRPKMFYSQRIHDKCYRRPHF
DAGQYVESFDDEGARKGYCLYKVGCKGPTTYNACSTVRWNDGVSFPIQAGHGCIGCSEDGFWDKGSWYARLSDLKNAGFG
IESNADQVGLIAAGAVGAGVAVHAAVSALKRAQHKNTDNGADTGATGGEK
>Mature_369_residues
ALETFYEVMRRQGITRRSFLKYCSLTAAALGLGPEFAPQIAEAMETKPRTPVLWLHGLECTCCSESFIRSAHPLAKDVVL
SMISLDYDDTLMASAGHQAEAILDEVMEKYKGNYILAVEGNPPLNEDGMYCIIGGKPFVDQLKKVAKDAKAIISWGSCAS
YGCVQAARPNPTRATPVHEVIFDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPDLDRQGRPKMFYSQRIHDKCYRRPHFD
AGQYVESFDDEGARKGYCLYKVGCKGPTTYNACSTVRWNDGVSFPIQAGHGCIGCSEDGFWDKGSWYARLSDLKNAGFGI
ESNADQVGLIAAGAVGAGVAVHAAVSALKRAQHKNTDNGADTGATGGEK

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=331, Percent_Identity=71.2990936555891, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1789371, Length=333, Percent_Identity=42.042042042042, Blast_Score=280, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 40242; Mature: 40111

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALETFYEVMRRQGITRRSFLKYCSLTAAALGLGPEFAPQIAEAMETKPRTPVLWLHGLE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCC
CTCCSESFIRSAHPLAKDVVLSMISLDYDDTLMASAGHQAEAILDEVMEKYKGNYILAVE
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
GNPPLNEDGMYCIIGGKPFVDQLKKVAKDAKAIISWGSCASYGCVQAARPNPTRATPVHE
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
VIFDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPDLDRQGRPKMFYSQRIHDKCYRRPHF
HHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DAGQYVESFDDEGARKGYCLYKVGCKGPTTYNACSTVRWNDGVSFPIQAGHGCIGCSEDG
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEECCCCEEECCCCC
FWDKGSWYARLSDLKNAGFGIESNADQVGLIAAGAVGAGVAVHAAVSALKRAQHKNTDNG
CCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
ADTGATGGEK
CCCCCCCCCC
>Mature Secondary Structure 
ALETFYEVMRRQGITRRSFLKYCSLTAAALGLGPEFAPQIAEAMETKPRTPVLWLHGLE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCC
CTCCSESFIRSAHPLAKDVVLSMISLDYDDTLMASAGHQAEAILDEVMEKYKGNYILAVE
CCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
GNPPLNEDGMYCIIGGKPFVDQLKKVAKDAKAIISWGSCASYGCVQAARPNPTRATPVHE
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
VIFDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPDLDRQGRPKMFYSQRIHDKCYRRPHF
HHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DAGQYVESFDDEGARKGYCLYKVGCKGPTTYNACSTVRWNDGVSFPIQAGHGCIGCSEDG
CHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCEECCCCEEECCCCC
FWDKGSWYARLSDLKNAGFGIESNADQVGLIAAGAVGAGVAVHAAVSALKRAQHKNTDNG
CCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
ADTGATGGEK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488; 2493816 [H]