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The map label for this gene is cysU [H]

Identifier: 154246094

GI number: 154246094

Start: 2412285

End: 2413103

Strand: Reverse

Name: cysU [H]

Synonym: Xaut_2151

Alternate gene names: 154246094

Gene position: 2413103-2412285 (Counterclockwise)

Preceding gene: 154246095

Following gene: 154246093

Centisome position: 45.45

GC content: 69.84

Gene sequence:

>819_bases
ATGCACAGCGCCGACCGCCTCGCCCGCCTGTTCCTGCTGCCGCTGTTCCTGTTCGTGGCGGCATTCTTCCTGCTGCCCAT
GGTGCGGCTCATCGGCGTGGCTGGCTCCGGGCCAAACGGCATGGCGGAATATCTCGCCATTCTCACCAACCCGCGTCACT
TCGCGACCCTCGTCGCCACCTTGACGCTCTCGGCCGCAGTGACGGCGGCGACGCTGGCCATCGGCACGGTGGTGGCCCTG
TTCCTCGCCCGCAACCGCTTCTTCGGGCGACCGCTGCTGGTGTCCCTGCTGACCCTGCCGCTGGCCTTTCCGGGCGTGGT
GGTGGGCTTCATGGTGATCCTGCTGGCGGGACGGCAGGGGCTGCTCTCCAGCCTCTCCATGGGACTCGGGCTGGGGCGGT
GGGTATTCGCCTATTCCATGGCCGGGCTGTTCGCCGGCTATGTCTATTTTTCCCTGCCCCGGGTGCTGGTCACCCTCATG
GCGGCGGTGGAGAAGCTGGACCCGGCGCTGGAGGAGGCCGCCCGCTCGCTGGGCGCCAGCCCGGCGCAGGTGCTGCGGGA
CGTGACCCTGCCCGGCATCGCCCCCGCGCTCGTGGCCTCCGGCGCCATTGCGTTTGCCACCGCCATGGGCGCCTTCGGCA
CCGCCTTCACGCTGGCGACACGCATCGATGTGCTGGCCATGACCATCTATACGGAGTTCACCCTGAACGCGAACTTCGCC
ACGGCCGCCGCCCTTTCCATCGCGCTCGGCCTCATCACCTGGGTGGTGCTCGCGCTGGCGCGCAATCTCGCGGGCGCCGG
CGTGGCGGCGGCGGGGTGA

Upstream 100 bases:

>100_bases
GCTACGAGCCGCGGCAAAGCCCTCTCCCGTTTGCGGGGGAGGGAGCCCGACTGTCCTTGCGCCGCCATCACCCCGGCACT
CTCCAACCCGGTCCCACCCC

Downstream 100 bases:

>100_bases
GGCCATGACCGCGTCGATGAAACCCCACCGCCTTACCTTCGCCTTCCAGCTGACGGTGACGCTTCTGGCCTGTGCCTTCC
TGATGGTGCCGGCGGGTGCC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MHSADRLARLFLLPLFLFVAAFFLLPMVRLIGVAGSGPNGMAEYLAILTNPRHFATLVATLTLSAAVTAATLAIGTVVAL
FLARNRFFGRPLLVSLLTLPLAFPGVVVGFMVILLAGRQGLLSSLSMGLGLGRWVFAYSMAGLFAGYVYFSLPRVLVTLM
AAVEKLDPALEEAARSLGASPAQVLRDVTLPGIAPALVASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNANFA
TAAALSIALGLITWVVLALARNLAGAGVAAAG

Sequences:

>Translated_272_residues
MHSADRLARLFLLPLFLFVAAFFLLPMVRLIGVAGSGPNGMAEYLAILTNPRHFATLVATLTLSAAVTAATLAIGTVVAL
FLARNRFFGRPLLVSLLTLPLAFPGVVVGFMVILLAGRQGLLSSLSMGLGLGRWVFAYSMAGLFAGYVYFSLPRVLVTLM
AAVEKLDPALEEAARSLGASPAQVLRDVTLPGIAPALVASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNANFA
TAAALSIALGLITWVVLALARNLAGAGVAAAG
>Mature_272_residues
MHSADRLARLFLLPLFLFVAAFFLLPMVRLIGVAGSGPNGMAEYLAILTNPRHFATLVATLTLSAAVTAATLAIGTVVAL
FLARNRFFGRPLLVSLLTLPLAFPGVVVGFMVILLAGRQGLLSSLSMGLGLGRWVFAYSMAGLFAGYVYFSLPRVLVTLM
AAVEKLDPALEEAARSLGASPAQVLRDVTLPGIAPALVASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNANFA
TAAALSIALGLITWVVLALARNLAGAGVAAAG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=33.0188679245283, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787570, Length=240, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787369, Length=169, Percent_Identity=31.9526627218935, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082099, Length=260, Percent_Identity=29.6153846153846, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786980, Length=217, Percent_Identity=34.5622119815668, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787368, Length=176, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786254, Length=191, Percent_Identity=28.7958115183246, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28159; Mature: 28159

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSADRLARLFLLPLFLFVAAFFLLPMVRLIGVAGSGPNGMAEYLAILTNPRHFATLVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
LTLSAAVTAATLAIGTVVALFLARNRFFGRPLLVSLLTLPLAFPGVVVGFMVILLAGRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLSSLSMGLGLGRWVFAYSMAGLFAGYVYFSLPRVLVTLMAAVEKLDPALEEAARSLGAS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
PAQVLRDVTLPGIAPALVASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNANFA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHH
TAAALSIALGLITWVVLALARNLAGAGVAAAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHSADRLARLFLLPLFLFVAAFFLLPMVRLIGVAGSGPNGMAEYLAILTNPRHFATLVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
LTLSAAVTAATLAIGTVVALFLARNRFFGRPLLVSLLTLPLAFPGVVVGFMVILLAGRQG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLSSLSMGLGLGRWVFAYSMAGLFAGYVYFSLPRVLVTLMAAVEKLDPALEEAARSLGAS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCC
PAQVLRDVTLPGIAPALVASGAIAFATAMGAFGTAFTLATRIDVLAMTIYTEFTLNANFA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHH
TAAALSIALGLITWVVLALARNLAGAGVAAAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]