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The map label for this gene is priA [H]

Identifier: 154245996

GI number: 154245996

Start: 2310675

End: 2312858

Strand: Reverse

Name: priA [H]

Synonym: Xaut_2053

Alternate gene names: 154245996

Gene position: 2312858-2310675 (Counterclockwise)

Preceding gene: 154246000

Following gene: 154245995

Centisome position: 43.57

GC content: 72.34

Gene sequence:

>2184_bases
ATGAGTGCGCGCACGGTCGATGTCCTCCTGCCCCTGGGCCTCGACACCCCCTATTCCTACCTCGTACCCGAGGGGATGAC
GCTCTCCCCCGGCGACCTCGTGCATGTGCCCCTGGGCGCGCGCGGGATGATCGGCTGCGTCTGGCCGCGTCCCGCCGGCG
GCGGATCGGGCAAGCCGGTGGATGTGGCCAAGCTCAAGGCGGTGAAGGCGAAGCTGGACTATGTCCCCCTGCCGCCGGAC
CTGTTGAAGCTCGTCGACTGGATGGCCGACTACACCCTCGCCCCGCGCGGCATGGTTCTGCGCATGGCCCTGCGCCACGG
CGAAAGCCCCGGCGCCGGCCGCGAGCGGGTGGGCGTGCGCGTCAGCGGCCCGGCCCCGGCGCGCATGACGGCGGCCCGCG
CCCGGCTGCTCGACCTGCTCGCCGACGGCTTCGTGCGCGGCAAGTCGGAGGCAGCGCGGGAGGCCGGGGTCTCCCCTTCC
GTGGTGGACGGGCTGGTGGACGAGGGCGCACTGGAAACCGTGGTGCTGCCCCCCGAGCCCGCCGCCTATCCCCCCGACCC
GACCTTCGCCACGCCCACCCTCTCCGAGCCGCAGGCGCAGGCGGCACACGCGCTCAGCGGAGCGGTGGACGCCAGGGGCT
TCGGCGTCTTCCTGCTGGACGGCGTCACCGGCTCGGGCAAGACGGAGGTGTATTTCGAGGCGGTGGCGCAGGCGATCCGC
GACGGCCGGCAGGCGCTGATCCTGCTGCCGGAAATCGCCCTCACCCAGGCCTTCCTCGACCGCTTCACCACCCGCTTCGG
GGTGAAGCCGGCGGAATGGCATTCCGGCGTCTCCACAAGGCGACGCGGGCGCATCCTCAACGAGGTGGCCTCAGGACAGG
TGAAGGTGGTGGCCGGCGCCCGTTCGGCCCTTTTCCTGCCATTCCGCGACCTCGGCCTCATCATCGTGGATGAGGAGCAC
GACCCGGCCTACAAGCAGGAGGACGGCGTCGCCTACCATGCCCGCGACATGGCGGTAGTGCGGGCGCGGCTGGCCTCCGC
CCCCATCGTGCTTGCCTCGGCCACCCCCTCCATCGAGACCGAGGTGAACGCCCGGCGCGGGCGCTACCATCGCCTCGCTT
TGCCGGTGCGCTTCGGCGGCGCCAAGGTGCCCGGCCTCCTGCCCATCGACCTGCGCAAGGAGGGCCCGCCCAAGGGCCGC
TGGATCGCGCCACGTCTCGAAGCGGAGATCACCGATACGCTGGAGGCGGGCGGACAGGCGCTGCTGTTCCTCAACCGACG
AGGCTATGCGCCGCTCACCCTGTGCCACTCCTGCGGGCACCGCATGCGCTGCCCCTCCTGCACCGCGTGGCTGGTGGAGC
ACCGCTTCCGCCGCCGCCTCACCTGCCACCATTGCGGCTATCAGGCCCCGGTGCCCGAGGCTTGCCCCGCCTGCGGCGCC
AAGGACAGCCTCATCCCCGTGGGGCCGGGGGTGGAGCGGCTGCAGGAGGAGGTTCAGGCGCTGTTTCCCGCTGCCCGTTC
CCTCGTCCTGTCCTCGGACCTCGCCGGCGGGGTGGAGCGCATGCGCGCCGAGCTGGACGCAGTGGCCAAGGGCGAGGTGG
ACGTGGTGGTGGGCACCCAGCTCGTAGCCAAGGGGCACAATTTCCCCGGCCTCGCACTGGTGGGCGTGGTGGACGCGGAT
GTGGGCCTCGGTCAGGGCGACCCGCGCGCGGCGGAACGCACCTTCCAGCTGGTGCACCAGGTGGCGGGGCGCGCCGGGCG
CGGCAAGACGGAAGGGCGCGGCTTCATCCAGACCCACATGCCCGAGCATCCGGTGATGCGGGCGCTGGTGACCGGCGACC
GCGACGCCTTCTACGATACCGAGATCGCCGCCCGGGAGGAGGCCCAGCTGCCCCCCTTCGGCCGCATGGCGAGCCTCATC
GTCTCCGCCAGCGAGGGCCATGTGGCGGAGGGCCATGCCCGAAGGCTCGCCGCCTCAGCCCCTTTGGTCGCGGGGGTACG
CATCCTTGGCCCGGCGGAAGCCCCGCTGGCGATCCTGCGCGGGCGCCATCGCTGGCGGCTGCTGGTGCGGGCACCCCGCG
CCTTCGACCTGTCGCGCTATCTGCGCGACTGGCGGGCCACGGCGCCGAAGGTGACGGGCAACATCCGCGTCGCCATCGAC
GTGGACCCGCAGAGCTTCTTTTGA

Upstream 100 bases:

>100_bases
CCATGGAAATGCATGCCGCTCCTGAAAGCGCCTTCGGCGCGCATCGAAGGCGGTCGGGCGCGCCTTTGACCTTTCCCGCC
GGACGGACCAAAACACCGCC

Downstream 100 bases:

>100_bases
GCCCCTGCCCGGCCGAGCACCCATGAAGCAGCGCCCCTCCTCCCGCCTGCTGGTCGTGAACGCGCAAGGTCGCCTGCTGC
TGTTCCGGTTCGCGCACAGG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MSARTVDVLLPLGLDTPYSYLVPEGMTLSPGDLVHVPLGARGMIGCVWPRPAGGGSGKPVDVAKLKAVKAKLDYVPLPPD
LLKLVDWMADYTLAPRGMVLRMALRHGESPGAGRERVGVRVSGPAPARMTAARARLLDLLADGFVRGKSEAAREAGVSPS
VVDGLVDEGALETVVLPPEPAAYPPDPTFATPTLSEPQAQAAHALSGAVDARGFGVFLLDGVTGSGKTEVYFEAVAQAIR
DGRQALILLPEIALTQAFLDRFTTRFGVKPAEWHSGVSTRRRGRILNEVASGQVKVVAGARSALFLPFRDLGLIIVDEEH
DPAYKQEDGVAYHARDMAVVRARLASAPIVLASATPSIETEVNARRGRYHRLALPVRFGGAKVPGLLPIDLRKEGPPKGR
WIAPRLEAEITDTLEAGGQALLFLNRRGYAPLTLCHSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEACPACGA
KDSLIPVGPGVERLQEEVQALFPAARSLVLSSDLAGGVERMRAELDAVAKGEVDVVVGTQLVAKGHNFPGLALVGVVDAD
VGLGQGDPRAAERTFQLVHQVAGRAGRGKTEGRGFIQTHMPEHPVMRALVTGDRDAFYDTEIAAREEAQLPPFGRMASLI
VSASEGHVAEGHARRLAASAPLVAGVRILGPAEAPLAILRGRHRWRLLVRAPRAFDLSRYLRDWRATAPKVTGNIRVAID
VDPQSFF

Sequences:

>Translated_727_residues
MSARTVDVLLPLGLDTPYSYLVPEGMTLSPGDLVHVPLGARGMIGCVWPRPAGGGSGKPVDVAKLKAVKAKLDYVPLPPD
LLKLVDWMADYTLAPRGMVLRMALRHGESPGAGRERVGVRVSGPAPARMTAARARLLDLLADGFVRGKSEAAREAGVSPS
VVDGLVDEGALETVVLPPEPAAYPPDPTFATPTLSEPQAQAAHALSGAVDARGFGVFLLDGVTGSGKTEVYFEAVAQAIR
DGRQALILLPEIALTQAFLDRFTTRFGVKPAEWHSGVSTRRRGRILNEVASGQVKVVAGARSALFLPFRDLGLIIVDEEH
DPAYKQEDGVAYHARDMAVVRARLASAPIVLASATPSIETEVNARRGRYHRLALPVRFGGAKVPGLLPIDLRKEGPPKGR
WIAPRLEAEITDTLEAGGQALLFLNRRGYAPLTLCHSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEACPACGA
KDSLIPVGPGVERLQEEVQALFPAARSLVLSSDLAGGVERMRAELDAVAKGEVDVVVGTQLVAKGHNFPGLALVGVVDAD
VGLGQGDPRAAERTFQLVHQVAGRAGRGKTEGRGFIQTHMPEHPVMRALVTGDRDAFYDTEIAAREEAQLPPFGRMASLI
VSASEGHVAEGHARRLAASAPLVAGVRILGPAEAPLAILRGRHRWRLLVRAPRAFDLSRYLRDWRATAPKVTGNIRVAID
VDPQSFF
>Mature_726_residues
SARTVDVLLPLGLDTPYSYLVPEGMTLSPGDLVHVPLGARGMIGCVWPRPAGGGSGKPVDVAKLKAVKAKLDYVPLPPDL
LKLVDWMADYTLAPRGMVLRMALRHGESPGAGRERVGVRVSGPAPARMTAARARLLDLLADGFVRGKSEAAREAGVSPSV
VDGLVDEGALETVVLPPEPAAYPPDPTFATPTLSEPQAQAAHALSGAVDARGFGVFLLDGVTGSGKTEVYFEAVAQAIRD
GRQALILLPEIALTQAFLDRFTTRFGVKPAEWHSGVSTRRRGRILNEVASGQVKVVAGARSALFLPFRDLGLIIVDEEHD
PAYKQEDGVAYHARDMAVVRARLASAPIVLASATPSIETEVNARRGRYHRLALPVRFGGAKVPGLLPIDLRKEGPPKGRW
IAPRLEAEITDTLEAGGQALLFLNRRGYAPLTLCHSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEACPACGAK
DSLIPVGPGVERLQEEVQALFPAARSLVLSSDLAGGVERMRAELDAVAKGEVDVVVGTQLVAKGHNFPGLALVGVVDADV
GLGQGDPRAAERTFQLVHQVAGRAGRGKTEGRGFIQTHMPEHPVMRALVTGDRDAFYDTEIAAREEAQLPPFGRMASLIV
SASEGHVAEGHARRLAASAPLVAGVRILGPAEAPLAILRGRHRWRLLVRAPRAFDLSRYLRDWRATAPKVTGNIRVAIDV
DPQSFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=745, Percent_Identity=35.5704697986577, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787357, Length=144, Percent_Identity=35.4166666666667, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 77907; Mature: 77776

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARTVDVLLPLGLDTPYSYLVPEGMTLSPGDLVHVPLGARGMIGCVWPRPAGGGSGKPV
CCCCEEEEEEECCCCCCCHHHCCCCCEECCCCEEEECCCCCCCEEEECCCCCCCCCCCCC
DVAKLKAVKAKLDYVPLPPDLLKLVDWMADYTLAPRGMVLRMALRHGESPGAGRERVGVR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHCCEE
VSGPAPARMTAARARLLDLLADGFVRGKSEAAREAGVSPSVVDGLVDEGALETVVLPPEP
ECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCC
AAYPPDPTFATPTLSEPQAQAAHALSGAVDARGFGVFLLDGVTGSGKTEVYFEAVAQAIR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
DGRQALILLPEIALTQAFLDRFTTRFGVKPAEWHSGVSTRRRGRILNEVASGQVKVVAGA
CCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
RSALFLPFRDLGLIIVDEEHDPAYKQEDGVAYHARDMAVVRARLASAPIVLASATPSIET
CCEEEEEHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCC
EVNARRGRYHRLALPVRFGGAKVPGLLPIDLRKEGPPKGRWIAPRLEAEITDTLEAGGQA
CCCCCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCCCEECCHHHHHHHHHHHCCCCE
LLFLNRRGYAPLTLCHSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEACPACGA
EEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCC
KDSLIPVGPGVERLQEEVQALFPAARSLVLSSDLAGGVERMRAELDAVAKGEVDVVVGTQ
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
LVAKGHNFPGLALVGVVDADVGLGQGDPRAAERTFQLVHQVAGRAGRGKTEGRGFIQTHM
CEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
PEHPVMRALVTGDRDAFYDTEIAAREEAQLPPFGRMASLIVSASEGHVAEGHARRLAASA
CCCHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHCC
PLVAGVRILGPAEAPLAILRGRHRWRLLVRAPRAFDLSRYLRDWRATAPKVTGNIRVAID
CHHHCEEEECCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
VDPQSFF
CCCCCCC
>Mature Secondary Structure 
SARTVDVLLPLGLDTPYSYLVPEGMTLSPGDLVHVPLGARGMIGCVWPRPAGGGSGKPV
CCCEEEEEEECCCCCCCHHHCCCCCEECCCCEEEECCCCCCCEEEECCCCCCCCCCCCC
DVAKLKAVKAKLDYVPLPPDLLKLVDWMADYTLAPRGMVLRMALRHGESPGAGRERVGVR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHCCEE
VSGPAPARMTAARARLLDLLADGFVRGKSEAAREAGVSPSVVDGLVDEGALETVVLPPEP
ECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCC
AAYPPDPTFATPTLSEPQAQAAHALSGAVDARGFGVFLLDGVTGSGKTEVYFEAVAQAIR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
DGRQALILLPEIALTQAFLDRFTTRFGVKPAEWHSGVSTRRRGRILNEVASGQVKVVAGA
CCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECC
RSALFLPFRDLGLIIVDEEHDPAYKQEDGVAYHARDMAVVRARLASAPIVLASATPSIET
CCEEEEEHHHCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCCC
EVNARRGRYHRLALPVRFGGAKVPGLLPIDLRKEGPPKGRWIAPRLEAEITDTLEAGGQA
CCCCCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCCCCCEECCHHHHHHHHHHHCCCCE
LLFLNRRGYAPLTLCHSCGHRMRCPSCTAWLVEHRFRRRLTCHHCGYQAPVPEACPACGA
EEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCC
KDSLIPVGPGVERLQEEVQALFPAARSLVLSSDLAGGVERMRAELDAVAKGEVDVVVGTQ
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
LVAKGHNFPGLALVGVVDADVGLGQGDPRAAERTFQLVHQVAGRAGRGKTEGRGFIQTHM
CEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
PEHPVMRALVTGDRDAFYDTEIAAREEAQLPPFGRMASLIVSASEGHVAEGHARRLAASA
CCCHHHHHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHCC
PLVAGVRILGPAEAPLAILRGRHRWRLLVRAPRAFDLSRYLRDWRATAPKVTGNIRVAID
CHHHCEEEECCCCCCHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
VDPQSFF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]