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The map label for this gene is pecT [H]

Identifier: 154245989

GI number: 154245989

Start: 2304274

End: 2305137

Strand: Direct

Name: pecT [H]

Synonym: Xaut_2046

Alternate gene names: 154245989

Gene position: 2304274-2305137 (Clockwise)

Preceding gene: 154245987

Following gene: 154245991

Centisome position: 43.4

GC content: 71.88

Gene sequence:

>864_bases
ATGGATCTGCTGAAGGCCTTCGTCAGCGTGGTGGATGCCGGCGGCTTCACCCGCGCCGGCGAGCGCGTCCATCGCACCCA
GTCCACGGTCAGCCAGCAGATCCGCCGCCTGGAGGACCAGCTCGGCCATCAGTTGCTGGTGCGGGATGCGCGCGGCGTGA
CCCCCACCGCCGAGGGCGAGCGGCTTCTGGGCTATGCCCGGCGGATTCTCGCCTTGGGGGCCGAGGCGAAAGCGGCACTC
TCGGGTGCTGCGCCGGCGCTGGTGGTGCGGCTCGGCATTCCAGACGATTTCGCGCTGGCGGCGCTGACCCAGGTGATCGC
CGATTTCGCCCGCGCCCGCCCCGGCGTGCGCCTCGCGGTGGAATGCGGGCTCAGCTGCGACATTGCCGCCGCCTTTGCCC
GCGGCGATCTCGACATCGCGCTGGTGAAGCGGGAGCCGTCGAGCGGGCCGGCCCTCGCGGTGTGGCCGGAGCGCCTTGTC
TGGGTCACGGGGCGCGACGTGGTGGTGGATCCGGACACCGTCCCGCTGGTGGCCTTCCGGCAGGGCTGCCTCTATCGCAA
CAGGGCGGTGCATGCGCTGGAAAAGGCTGGCCGGGCGTGGCGCATCGCCTATGAATGCCCGAGCCTCATCGGCATCGACG
CGGCGCTCGCCGGAGGGCTCGGCGTGGCGGTGCTGAGCACCTTGTCGCTCTATCGTCGCGAGCCGAGCCTGCGGGTGCTG
TCGGCAGCCGAGCTGTGGCCGCAGATCCCGCCCACGGAACTGGCTTTGCGGATTGCCGACCCCGCCTGCGCCGCCACCCG
CGACCTTGCCGCTTTGGTGGCGGGCTTCTGCGACCGTGAAGCCGCCTGCGCGGCTGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGGACCCGCCTGCGCCAGAGACCACGAAATGCGAGGATTGAAAAATTGAATGTCGTGATGTTAGCTAGTGACTAATCGA
ATGGGTGAACGATGCTGGAC

Downstream 100 bases:

>100_bases
GGGGCGCCTGCCCTACGCGTGCTCTAGTCGTCCACCAGCAGCCGCCGGCCGATCTCGCCGATGCCGATCAGGCCGGGGGT
GGCGTGCGTCACCACATGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDLLKAFVSVVDAGGFTRAGERVHRTQSTVSQQIRRLEDQLGHQLLVRDARGVTPTAEGERLLGYARRILALGAEAKAAL
SGAAPALVVRLGIPDDFALAALTQVIADFARARPGVRLAVECGLSCDIAAAFARGDLDIALVKREPSSGPALAVWPERLV
WVTGRDVVVDPDTVPLVAFRQGCLYRNRAVHALEKAGRAWRIAYECPSLIGIDAALAGGLGVAVLSTLSLYRREPSLRVL
SAAELWPQIPPTELALRIADPACAATRDLAALVAGFCDREAACAAAA

Sequences:

>Translated_287_residues
MDLLKAFVSVVDAGGFTRAGERVHRTQSTVSQQIRRLEDQLGHQLLVRDARGVTPTAEGERLLGYARRILALGAEAKAAL
SGAAPALVVRLGIPDDFALAALTQVIADFARARPGVRLAVECGLSCDIAAAFARGDLDIALVKREPSSGPALAVWPERLV
WVTGRDVVVDPDTVPLVAFRQGCLYRNRAVHALEKAGRAWRIAYECPSLIGIDAALAGGLGVAVLSTLSLYRREPSLRVL
SAAELWPQIPPTELALRIADPACAATRDLAALVAGFCDREAACAAAA
>Mature_287_residues
MDLLKAFVSVVDAGGFTRAGERVHRTQSTVSQQIRRLEDQLGHQLLVRDARGVTPTAEGERLLGYARRILALGAEAKAAL
SGAAPALVVRLGIPDDFALAALTQVIADFARARPGVRLAVECGLSCDIAAAFARGDLDIALVKREPSSGPALAVWPERLV
WVTGRDVVVDPDTVPLVAFRQGCLYRNRAVHALEKAGRAWRIAYECPSLIGIDAALAGGLGVAVLSTLSLYRREPSLRVL
SAAELWPQIPPTELALRIADPACAATRDLAALVAGFCDREAACAAAA

Specific function: Regulates pectinase gene expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=274, Percent_Identity=31.3868613138686, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787806, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788297, Length=235, Percent_Identity=31.063829787234, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787589, Length=276, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=167, Percent_Identity=33.5329341317365, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=31.7241379310345, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788296, Length=231, Percent_Identity=26.4069264069264, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=29.6774193548387, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30372; Mature: 30372

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLKAFVSVVDAGGFTRAGERVHRTQSTVSQQIRRLEDQLGHQLLVRDARGVTPTAEGE
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHCCCCCCCCCCH
RLLGYARRILALGAEAKAALSGAAPALVVRLGIPDDFALAALTQVIADFARARPGVRLAV
HHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
ECGLSCDIAAAFARGDLDIALVKREPSSGPALAVWPERLVWVTGRDVVVDPDTVPLVAFR
ECCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEECCCEEEEEECCEEEECCCCCHHHHHH
QGCLYRNRAVHALEKAGRAWRIAYECPSLIGIDAALAGGLGVAVLSTLSLYRREPSLRVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEE
SAAELWPQIPPTELALRIADPACAATRDLAALVAGFCDREAACAAAA
EHHHHCCCCCCHHHEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MDLLKAFVSVVDAGGFTRAGERVHRTQSTVSQQIRRLEDQLGHQLLVRDARGVTPTAEGE
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHCCCCCCCCCCH
RLLGYARRILALGAEAKAALSGAAPALVVRLGIPDDFALAALTQVIADFARARPGVRLAV
HHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEE
ECGLSCDIAAAFARGDLDIALVKREPSSGPALAVWPERLVWVTGRDVVVDPDTVPLVAFR
ECCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEECCCEEEEEECCEEEECCCCCHHHHHH
QGCLYRNRAVHALEKAGRAWRIAYECPSLIGIDAALAGGLGVAVLSTLSLYRREPSLRVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHEE
SAAELWPQIPPTELALRIADPACAATRDLAALVAGFCDREAACAAAA
EHHHHCCCCCCHHHEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]