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The map label for this gene is ptsN [H]

Identifier: 154245977

GI number: 154245977

Start: 2293495

End: 2293959

Strand: Reverse

Name: ptsN [H]

Synonym: Xaut_2034

Alternate gene names: 154245977

Gene position: 2293959-2293495 (Counterclockwise)

Preceding gene: 154245978

Following gene: 154245970

Centisome position: 43.21

GC content: 66.02

Gene sequence:

>465_bases
ATGCCTCTCGCCGACCTTCTCGCCCCGGAAGCCATTTTTCCCACGCTGCGGGCGAGCAGCAAGAAACAGGCTCTCCAGGA
ATTGTCGCACCACGCGTCCGTCCTGATGGGCCGCGACCAGCGGGAGATCTTCGAAACCCTTCTCCAGCGCGAGCGCCTCG
GATCCACAGGCGTGGGTAATGGCATCGCCATCCCCCATGGCAAGCTGCCGGGGCTGACCAAGCTGTTCGGACTGTTCGCG
CGACTGGAAAAGCCCATCGATTTCGAATCGCTGGACGGCGCGCCGGTGGATCTGGTCTTCCTGCTGCTTGCGCCGGAAGC
CGCCGGCGCGGACCATCTGAAGGCGCTAGCCCGCGTCGCCCGCATGCTGCGGGATCCGGACGTGGTGGAAAAGCTGCGCG
CCAGCCGCGACGCCACATCTATCCACACACTGCTCACGGCGGCGTCGGCCTCCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
TCCGGGCAGGGAAGCAGAGGCCTGCTTGCCTGCCCGGAATCCGTCTCTCGCGTCCGCCGCTTATTTCGAGCCTCGGCCGC
TCGCCGCAGGGATCCGATCC

Downstream 100 bases:

>100_bases
CCGGCGTATCCCTGCTTCTGAGGACGCGAATCAGCTGCATGCCGCAAGCCCCCGGTCCAATGCGAACCGGCGACCCGCGG
CAGTTGGCTGTCTCGTCACT

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MPLADLLAPEAIFPTLRASSKKQALQELSHHASVLMGRDQREIFETLLQRERLGSTGVGNGIAIPHGKLPGLTKLFGLFA
RLEKPIDFESLDGAPVDLVFLLLAPEAAGADHLKALARVARMLRDPDVVEKLRASRDATSIHTLLTAASASNAA

Sequences:

>Translated_154_residues
MPLADLLAPEAIFPTLRASSKKQALQELSHHASVLMGRDQREIFETLLQRERLGSTGVGNGIAIPHGKLPGLTKLFGLFA
RLEKPIDFESLDGAPVDLVFLLLAPEAAGADHLKALARVARMLRDPDVVEKLRASRDATSIHTLLTAASASNAA
>Mature_153_residues
PLADLLAPEAIFPTLRASSKKQALQELSHHASVLMGRDQREIFETLLQRERLGSTGVGNGIAIPHGKLPGLTKLFGLFAR
LEKPIDFESLDGAPVDLVFLLLAPEAAGADHLKALARVARMLRDPDVVEKLRASRDATSIHTLLTAASASNAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=147, Percent_Identity=39.4557823129252, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788726, Length=142, Percent_Identity=40.1408450704225, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI2367327, Length=95, Percent_Identity=33.6842105263158, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786951, Length=150, Percent_Identity=32, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16507; Mature: 16376

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLADLLAPEAIFPTLRASSKKQALQELSHHASVLMGRDQREIFETLLQRERLGSTGVGN
CCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLPGLTKLFGLFARLEKPIDFESLDGAPVDLVFLLLAPEAAGADHLKALARVA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RMLRDPDVVEKLRASRDATSIHTLLTAASASNAA
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLADLLAPEAIFPTLRASSKKQALQELSHHASVLMGRDQREIFETLLQRERLGSTGVGN
CHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLPGLTKLFGLFARLEKPIDFESLDGAPVDLVFLLLAPEAAGADHLKALARVA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
RMLRDPDVVEKLRASRDATSIHTLLTAASASNAA
HHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]