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The map label for this gene is potD-B [H]

Identifier: 154244813

GI number: 154244813

Start: 961471

End: 962538

Strand: Direct

Name: potD-B [H]

Synonym: Xaut_0863

Alternate gene names: 154244813

Gene position: 961471-962538 (Clockwise)

Preceding gene: 154244812

Following gene: 154244814

Centisome position: 18.11

GC content: 64.33

Gene sequence:

>1068_bases
ATGAGCGACAAGATGAACATGTCTCGCCGCACCGCGCTGAAGACGTTTGGCGTGGCTGCCGCCGGGGCGCTGGCGGCGCC
CGCCGTCATCACCCGGTCGCTGGCCGCCAGCGAGACGCTGGTGGTCTATAATTTCGATGGTCTGCTCGGCAAGGTCATCA
AGGATCACTGGATCGATCCCTTCAGCCAGTCCACCGGGGTGAAGGTCGAAGTGCTGACCATGCAGGGCTCCTCCCCGCCC
ATGGCCAAGATCAAGGCCCAGGTGGATGCCGGCCGCCCCGATGCGGATGTCATCCCCATGCAGATGCCCGACTATGTCTT
CGGCGTGCGGAACAACCTGTTCCAGACCATCGACGCCAAGGACATGCCCGAATACGCCAACCTCTATCCCGATTACATCA
CGCCTCATGGGCCGAAACTGGTGCTGTGGAGCTACGGCCTCGCCTACAACACCGACAAGATCAAGACCGCGCCCACCGCC
TGGGCCGACCTGTGGGACCCCCAGTACAAGGGCAAGGCGGCGCTGAACGAGGCGCTGTTCGACCAGGCGCTACAGATGGT
CAACCTCGTCGCCAAGGGCAAGCCTTTGCCGGTGGACGACGCCACCTTCGCCGCCCTCACCAGGCTGCGCCCGAATCTTG
CAACGCTCTGGACCACAGGCGCCCAAGCCGAGCAATTGCTGCGCACCGGCGAGGTGTGGATCATGCCGGTGTGGAACGGC
CGCGTGTATCCGCTCAAGGACCAGGGCGTGCCGATTGATTTCGTCTCGCCGAAGGAAGGCTTCTTCACCCGGGCCGACCC
GTTCTGCATTCCGCGCGGCGCGAAGAACCCGGAGCTTGCCAAGGCCTTCATCAACTTCTCCTGCACGGTCGCCCCGCAGC
AGGCGCTGGCCAAGGGGCTGTTCTATGCCTCGCCCAACCAGAAGGTGGTCTATCCGCCGGAAATCGCCAAGCGCGTGGTG
GTCTCCACCCGCGAGGAACTGGCGCGCGCCGTGCCGGAGGATTTCGAGGTGATCGTGGACAATCTGCCCGATTGGCGCCG
GCGCTGGGATGCCTGGAAGCAGAGCTGA

Upstream 100 bases:

>100_bases
GTCAAGCCCGCCGTCTGAGGCGGGCGTCTTCTTCAATAAGACCTGAATTAGCGACCCAAATATGGGCGCACCTTCCATCC
GACCAGAGGACTTGAGGAAC

Downstream 100 bases:

>100_bases
GCGAAAGCCGGTCGCGGAGCCCTCGTCATGGCGAGCGATGCCTCTCTCCCATCCTCCCTCGCGGATCCACGGGGCAAGTG
GATGTCATTGAAAAACCTCG

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSDKMNMSRRTALKTFGVAAAGALAAPAVITRSLAASETLVVYNFDGLLGKVIKDHWIDPFSQSTGVKVEVLTMQGSSPP
MAKIKAQVDAGRPDADVIPMQMPDYVFGVRNNLFQTIDAKDMPEYANLYPDYITPHGPKLVLWSYGLAYNTDKIKTAPTA
WADLWDPQYKGKAALNEALFDQALQMVNLVAKGKPLPVDDATFAALTRLRPNLATLWTTGAQAEQLLRTGEVWIMPVWNG
RVYPLKDQGVPIDFVSPKEGFFTRADPFCIPRGAKNPELAKAFINFSCTVAPQQALAKGLFYASPNQKVVYPPEIAKRVV
VSTREELARAVPEDFEVIVDNLPDWRRRWDAWKQS

Sequences:

>Translated_355_residues
MSDKMNMSRRTALKTFGVAAAGALAAPAVITRSLAASETLVVYNFDGLLGKVIKDHWIDPFSQSTGVKVEVLTMQGSSPP
MAKIKAQVDAGRPDADVIPMQMPDYVFGVRNNLFQTIDAKDMPEYANLYPDYITPHGPKLVLWSYGLAYNTDKIKTAPTA
WADLWDPQYKGKAALNEALFDQALQMVNLVAKGKPLPVDDATFAALTRLRPNLATLWTTGAQAEQLLRTGEVWIMPVWNG
RVYPLKDQGVPIDFVSPKEGFFTRADPFCIPRGAKNPELAKAFINFSCTVAPQQALAKGLFYASPNQKVVYPPEIAKRVV
VSTREELARAVPEDFEVIVDNLPDWRRRWDAWKQS
>Mature_354_residues
SDKMNMSRRTALKTFGVAAAGALAAPAVITRSLAASETLVVYNFDGLLGKVIKDHWIDPFSQSTGVKVEVLTMQGSSPPM
AKIKAQVDAGRPDADVIPMQMPDYVFGVRNNLFQTIDAKDMPEYANLYPDYITPHGPKLVLWSYGLAYNTDKIKTAPTAW
ADLWDPQYKGKAALNEALFDQALQMVNLVAKGKPLPVDDATFAALTRLRPNLATLWTTGAQAEQLLRTGEVWIMPVWNGR
VYPLKDQGVPIDFVSPKEGFFTRADPFCIPRGAKNPELAKAFINFSCTVAPQQALAKGLFYASPNQKVVYPPEIAKRVVV
STREELARAVPEDFEVIVDNLPDWRRRWDAWKQS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=273, Percent_Identity=29.6703296703297, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39390; Mature: 39258

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKMNMSRRTALKTFGVAAAGALAAPAVITRSLAASETLVVYNFDGLLGKVIKDHWIDP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
FSQSTGVKVEVLTMQGSSPPMAKIKAQVDAGRPDADVIPMQMPDYVFGVRNNLFQTIDAK
CCCCCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCC
DMPEYANLYPDYITPHGPKLVLWSYGLAYNTDKIKTAPTAWADLWDPQYKGKAALNEALF
CCCHHHHCCCCCCCCCCCEEEEEECCCEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHH
DQALQMVNLVAKGKPLPVDDATFAALTRLRPNLATLWTTGAQAEQLLRTGEVWIMPVWNG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHEEEECCHHHHHHHHCCCEEEEEECCC
RVYPLKDQGVPIDFVSPKEGFFTRADPFCIPRGAKNPELAKAFINFSCTVAPQQALAKGL
EEEEECCCCCCEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHC
FYASPNQKVVYPPEIAKRVVVSTREELARAVPEDFEVIVDNLPDWRRRWDAWKQS
EEECCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SDKMNMSRRTALKTFGVAAAGALAAPAVITRSLAASETLVVYNFDGLLGKVIKDHWIDP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCC
FSQSTGVKVEVLTMQGSSPPMAKIKAQVDAGRPDADVIPMQMPDYVFGVRNNLFQTIDAK
CCCCCCCEEEEEEECCCCCCHHHEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCC
DMPEYANLYPDYITPHGPKLVLWSYGLAYNTDKIKTAPTAWADLWDPQYKGKAALNEALF
CCCHHHHCCCCCCCCCCCEEEEEECCCEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHH
DQALQMVNLVAKGKPLPVDDATFAALTRLRPNLATLWTTGAQAEQLLRTGEVWIMPVWNG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHEEEECCHHHHHHHHCCCEEEEEECCC
RVYPLKDQGVPIDFVSPKEGFFTRADPFCIPRGAKNPELAKAFINFSCTVAPQQALAKGL
EEEEECCCCCCEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHHHHC
FYASPNQKVVYPPEIAKRVVVSTREELARAVPEDFEVIVDNLPDWRRRWDAWKQS
EEECCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]