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The map label for this gene is braE [H]

Identifier: 154244595

GI number: 154244595

Start: 722967

End: 724010

Strand: Reverse

Name: braE [H]

Synonym: Xaut_0643

Alternate gene names: 154244595

Gene position: 724010-722967 (Counterclockwise)

Preceding gene: 154244596

Following gene: 154244594

Centisome position: 13.64

GC content: 68.01

Gene sequence:

>1044_bases
ATGACGCGCTTCATCGCCCCCCTCTTCCTCGTGGTCTTCGCCCTGGTCCCGCTCGGGCTCAAGGACCCCTATCTGCTCAA
TGCCCTCATCATCACGGGCATCCTCACCATCTGCGCCATGAGCCTGAACCTGCTGCTCGGCTTCACCGGGCAGCTCAGCC
TCGGGCACGTGGCCTTCTTCGGCATCGGCGCCTATGTGAGCGCGCTCACCGCTCTGGGCTTCGACGTGAGCCTGCCGTTC
GGTCTGCGGGTGACCCACGAGGGCGGATCGCCGCTGATGGGCTTCGTGCTGGCCGTCGGCCTTACGGGACTGTGCGGCTA
TCTCATCGGCCGGCTGTCGTTCCGGGTGCGGGGCGCCTATTTCGTCATCGTCACCATCTCGTTCGCCGAGGTGGTGCGGC
TGGTGGCGCTGAACTGGGTGGAGCTGACGCAGGGCCCGCTCGCCCTCACCGCCATCCCCAACATGGTGCTGCCGGTGCCC
GGCCTCGGCGACGTGGCGCTGCGCTCCAAGATGCAGAACTACTACCTGGTGCTGGCGGTGGCGGCGGTGAGCTATCTCAT
CATCGCCCGGCTGGTGGCCTCGCGCTTCGGCAGGGCCATGCGCGGGCTGAAGGAGAACGAATCCCTCGCCCTCTCCCTCG
GCATCAACGCCACCCGCACCCTCACCGTGGCGGCCACCATCTCCGCCGCCATGGCCGGGGCGGCGGGCAGCCTCTATGCC
CATTACATCCGCATCATCGATCCGGACGTGTTCCTGTTCTCCTACACCGTCACCATGGTCATCATGGTGGTGTCGGGCGG
CAAGGGCACGCTGGCCGGCCCGCTGGTGGGCGGCATCCTGTTCGGCCTGCTGCCGGTGGCGCTGCGGCCGGTGATGGCGC
CGGAAGCGCAATGGATCGTCTACGGCGCCATCCTCATCGCCATCCTGTTCCTGATGCCACGGGGCATCGTGCCGGCTCTG
GAGAGCCTCGCCCTCCGCCGCAGGCCGGCGGCCGCCGAGGCCAAGATCCCGGCAAAGAACGCCGAAGCGGGAGCCAACCC
ATGA

Upstream 100 bases:

>100_bases
ACATCTCCTCGGGCTATCGCGACGCCATGGGCTTCCTCATCATCATCGCGGTGCTGCTGGTGAAGCCCACCGGCCTGTTC
GCCCGCGCGGAGCGGATCGG

Downstream 100 bases:

>100_bases
GCACGCGTGAGAGCACGCCCATGAGCCATCCTGCGGGCACGGCCATCCTCAATGTGGAGCATGTGGGCATGCGGTTCGGT
GGCCTCACCGCCATCGCCGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTRFIAPLFLVVFALVPLGLKDPYLLNALIITGILTICAMSLNLLLGFTGQLSLGHVAFFGIGAYVSALTALGFDVSLPF
GLRVTHEGGSPLMGFVLAVGLTGLCGYLIGRLSFRVRGAYFVIVTISFAEVVRLVALNWVELTQGPLALTAIPNMVLPVP
GLGDVALRSKMQNYYLVLAVAAVSYLIIARLVASRFGRAMRGLKENESLALSLGINATRTLTVAATISAAMAGAAGSLYA
HYIRIIDPDVFLFSYTVTMVIMVVSGGKGTLAGPLVGGILFGLLPVALRPVMAPEAQWIVYGAILIAILFLMPRGIVPAL
ESLALRRRPAAAEAKIPAKNAEAGANP

Sequences:

>Translated_347_residues
MTRFIAPLFLVVFALVPLGLKDPYLLNALIITGILTICAMSLNLLLGFTGQLSLGHVAFFGIGAYVSALTALGFDVSLPF
GLRVTHEGGSPLMGFVLAVGLTGLCGYLIGRLSFRVRGAYFVIVTISFAEVVRLVALNWVELTQGPLALTAIPNMVLPVP
GLGDVALRSKMQNYYLVLAVAAVSYLIIARLVASRFGRAMRGLKENESLALSLGINATRTLTVAATISAAMAGAAGSLYA
HYIRIIDPDVFLFSYTVTMVIMVVSGGKGTLAGPLVGGILFGLLPVALRPVMAPEAQWIVYGAILIAILFLMPRGIVPAL
ESLALRRRPAAAEAKIPAKNAEAGANP
>Mature_346_residues
TRFIAPLFLVVFALVPLGLKDPYLLNALIITGILTICAMSLNLLLGFTGQLSLGHVAFFGIGAYVSALTALGFDVSLPFG
LRVTHEGGSPLMGFVLAVGLTGLCGYLIGRLSFRVRGAYFVIVTISFAEVVRLVALNWVELTQGPLALTAIPNMVLPVPG
LGDVALRSKMQNYYLVLAVAAVSYLIIARLVASRFGRAMRGLKENESLALSLGINATRTLTVAATISAAMAGAAGSLYAH
YIRIIDPDVFLFSYTVTMVIMVVSGGKGTLAGPLVGGILFGLLPVALRPVMAPEAQWIVYGAILIAILFLMPRGIVPALE
SLALRRRPAAAEAKIPAKNAEAGANP

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=328, Percent_Identity=29.5731707317073, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789866, Length=311, Percent_Identity=28.6173633440514, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36524; Mature: 36393

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFIAPLFLVVFALVPLGLKDPYLLNALIITGILTICAMSLNLLLGFTGQLSLGHVAFF
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIGAYVSALTALGFDVSLPFGLRVTHEGGSPLMGFVLAVGLTGLCGYLIGRLSFRVRGAY
HHHHHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEE
FVIVTISFAEVVRLVALNWVELTQGPLALTAIPNMVLPVPGLGDVALRSKMQNYYLVLAV
EEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHH
AAVSYLIIARLVASRFGRAMRGLKENESLALSLGINATRTLTVAATISAAMAGAAGSLYA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHCCCHHHHH
HYIRIIDPDVFLFSYTVTMVIMVVSGGKGTLAGPLVGGILFGLLPVALRPVMAPEAQWIV
HHHHHCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YGAILIAILFLMPRGIVPALESLALRRRPAAAEAKIPAKNAEAGANP
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRFIAPLFLVVFALVPLGLKDPYLLNALIITGILTICAMSLNLLLGFTGQLSLGHVAFF
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
GIGAYVSALTALGFDVSLPFGLRVTHEGGSPLMGFVLAVGLTGLCGYLIGRLSFRVRGAY
HHHHHHHHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEE
FVIVTISFAEVVRLVALNWVELTQGPLALTAIPNMVLPVPGLGDVALRSKMQNYYLVLAV
EEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHH
AAVSYLIIARLVASRFGRAMRGLKENESLALSLGINATRTLTVAATISAAMAGAAGSLYA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHCCCHHHHH
HYIRIIDPDVFLFSYTVTMVIMVVSGGKGTLAGPLVGGILFGLLPVALRPVMAPEAQWIV
HHHHHCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
YGAILIAILFLMPRGIVPALESLALRRRPAAAEAKIPAKNAEAGANP
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]