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The map label for this gene is ppdK [H]

Identifier: 154244451

GI number: 154244451

Start: 547633

End: 550293

Strand: Reverse

Name: ppdK [H]

Synonym: Xaut_0494

Alternate gene names: 154244451

Gene position: 550293-547633 (Counterclockwise)

Preceding gene: 154244452

Following gene: 154244450

Centisome position: 10.37

GC content: 68.13

Gene sequence:

>2661_bases
ATGACGAAATGGGTCTACACCTTCGGCGACGGGAAGGCCGAGGGCAAAGCCGACATGAAGAACCTGCTCGGCGGCAAGGG
CGCGAACCTCGCCGAGATGTCCAATCTCGGCTTGCCAGTCCCTCCCGGGTTCACCATTCCTACCGAGGTGTGTACCTATT
ATTACGCACACGGCCAGACCTATCCGGCCGAGCTGAAGGGCGACGTTGAAGCCGCCCTGGCCCAGGTCGGCCAGCTCACC
GGCAAGTCGTTCGGCGATGCCGCCAACCCGCTGCTGGTCTCGGTGCGCTCCGGCGCCCGCGCCTCCATGCCGGGCATGAT
GGACACCGTGCTCAACCTCGGCCTCAACGACGAGACTGTGGAAGCCGTGGCCAGGCTCGCCGGCGACCGGCGCTTCGCCT
ATGACAGCTACCGCCGCTTCATCACCATGTACTCGGACGTGGTGCTGGGCGTGAGCCACCACCACTTCGAGGAGATCCTG
GAGCACTACAAGCTCCAGAAGGGCTTCAACCTCGACACCGAGCTGTCCGCCGACGACTGGGCGGCGCTGGTGGTGAAGTA
CAAGGAGAAGGTCGCGGCCGAGCTCGGCAAGCCCTTCCCGCAGGATCCCCACGAGCAGCTGTGGGGCGCCATCGGCGCCG
TGTTCAGCTCGTGGATGAACGCCCGCGCCATCACCTACCGCCGCCTGCACTCCATTCCGGAGAGCTGGGGCACCGCGGTG
AACGTGCAGTCCATGGTGTTCGGCAACATGGGCGAGACCTCGGCCACCGGCGTCGCCTTCACCCGCAACCCGTCCACCGG
CGAGAACGCGCTTTACGGCGAGTTCCTGGTGAACGCCCAGGGCGAGGACGTGGTGGCGGGCATCCGCACCCCGCAGGACC
TGACCGAGAAGGCCCGCAAGACCGCCGGCTCGGACAAGCCCTCCATGGAGCTGGCCCTTCCCGAGGCCTTCAAGGAATTC
GAGCGCATCTCCCACGTGCTCGAAAACCACTACCGTGACATGCAGGACCTTGAGTTCACGGTGGAGAAGGGCAAGCTCTG
GATGCTCCAGACCCGCTCCGGCAAGCGCACCGCCAAGGCGGCCCTGCGCATCGCCGTGGAGCTGGCCGCCGAGGGCCTCA
TCACCGAGGAGGAGGCGATCGGCCGTGTCGATCCCGCCGCCCTCGACCAGCTGCTGCATCCGGCCATCGACCCCAAGGCC
GAGCGCAAGGTGATCGCCACCGGCCTGCCGGCCTCCCCCGGCGCGGCGGCGGGCGCCATCGTGTTCACCGCCGACGAGGC
GGAAGCGGCCAAGGAAAAGGGCCAGAAGGTCATCCTGGTGCGCGTGGAGACCTCTCCTGAGGACATCCATGGCATGCATG
CGGCCCAGGGCATCCTCACCGCCCGTGGCGGCATGACCTCCCACGCGGCCGTGGTGGCCCGCGGCATGGGCAAGCCCTGC
GTCTCCGGCGCCGGCTCCCTGCGCATCGATGCCGCCGCCCAGACCCTGACCGTGAACGGCCAGGTGTTCAAGAAGGGCGA
CCACATCACCATCGACGGCGGCACCGGGCAGGTGCTCTCCGGCGAGGTCGCCATGCTCCAGCCGGAGCTGTCGGGCGAGT
TCGCAACCCTGATGGGCTGGGCCGACAAGGTGCGCAAGCTGAAGGTGCGCGCCAATGCCGAGACCCCGGCGGATGCCCGC
ATGGCCCGCTCGTTCGGCGCCGAGGGCATCGGCCTCAGCCGCACCGAGCACATGTTCTTCGACGCCGGCCGCATCCTGGC
CGTGCGCGAGATGATCCTCGCGGACGACGAGGCCGGCCGCCGCGCGGCGCTGGCCAAGCTGCTGCCCATGCAGCGCGACG
ACTTCGTCGAGCTGTTCGAGATCATGAAGGGCCTGCCGGTCACCATCCGCCTGCTCGACCCGCCGCTCCACGAGTTCCTG
CCGCACACCGCGGAGGAAACCGAGGAGGTGGCCAAGAGCCTCGGCGTGGACGTGGAGCGCATCGAGCGGCGCAACAAGGA
GCTGCACGAGGTCAACCCGATGCTCGGGTTCCGCGGCTGCCGCATCGCCATCGCCTTCCCGGAGATCGCGGAAATGCAGG
CCCGCGCCATCTTCGAGGCGGCCATCATCGCCGCCAAGAAGACCGGCGAGGCCGTGGTACCCGAGATCATGGTGCCGCTC
ATCGCCACCAAGGCGGAGTTCGACATCGTGGACGCCGTGATCCGCAAGACCGCCGATGCGGTCGAGAAGGAGACCGGCGC
GAAGCTCACCTTCCAGGTGGGCACCATGATCGAGCTGCCCCGCGCCGCCCTGCGCGCGGAAGAGATCGCCAAGGGCGCCG
AGTTCTTCTCCTTCGGCACCAACGACCTCACCCAGACCACCTTCGGCGTCTCGCGCGACGACGCGGGCACCTTCCTCGGC
CCCTACGTTCAGAAGGGCATCATCGAGGTGGACCCGTTCGTCTCCATCGACGTGGACGGCGTGGGCGAGCTGGTGAAGAT
CGGCGTGGAACGCGGCCGCAAGTCGCGTCCCGACATCAAGCTTGGCATCTGCGGCGAGCACGGCGGTGATCCCGCCTCGG
TGGCCTTCTGCCACGAGGTAGGGCTCAACTACGTGTCCTGCTCGCCCTTCCGCGTGCCGATCGCCCGCCTCGCGGCGGCG
CAGGCGGCGCTGAAGAAGTAG

Upstream 100 bases:

>100_bases
GGGCAGGCAGGGGCTGCGGCGATGGCCCCTGTTGATGCTTTGTGCGCTGCACGCTAAGCATCTGCCCATTCCGCACATCC
CAGCTACAGGGATCAGCTAC

Downstream 100 bases:

>100_bases
GAACAAGGTCCGGAGGGGCGCGCTCCGCCCCACCCCAATAAAAGAGGCCGCCCGGACGACATGTCCGGGCGGCCTCTTCG
TTTTCATGCCGACGCGACCT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MTKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTIPTEVCTYYYAHGQTYPAELKGDVEAALAQVGQLT
GKSFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVARLAGDRRFAYDSYRRFITMYSDVVLGVSHHHFEEIL
EHYKLQKGFNLDTELSADDWAALVVKYKEKVAAELGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAV
NVQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARKTAGSDKPSMELALPEAFKEF
ERISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKAALRIAVELAAEGLITEEEAIGRVDPAALDQLLHPAIDPKA
ERKVIATGLPASPGAAAGAIVFTADEAEAAKEKGQKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPC
VSGAGSLRIDAAAQTLTVNGQVFKKGDHITIDGGTGQVLSGEVAMLQPELSGEFATLMGWADKVRKLKVRANAETPADAR
MARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRAALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFL
PHTAEETEEVAKSLGVDVERIERRNKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAIIAAKKTGEAVVPEIMVPL
IATKAEFDIVDAVIRKTADAVEKETGAKLTFQVGTMIELPRAALRAEEIAKGAEFFSFGTNDLTQTTFGVSRDDAGTFLG
PYVQKGIIEVDPFVSIDVDGVGELVKIGVERGRKSRPDIKLGICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAA
QAALKK

Sequences:

>Translated_886_residues
MTKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTIPTEVCTYYYAHGQTYPAELKGDVEAALAQVGQLT
GKSFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVARLAGDRRFAYDSYRRFITMYSDVVLGVSHHHFEEIL
EHYKLQKGFNLDTELSADDWAALVVKYKEKVAAELGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAV
NVQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARKTAGSDKPSMELALPEAFKEF
ERISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKAALRIAVELAAEGLITEEEAIGRVDPAALDQLLHPAIDPKA
ERKVIATGLPASPGAAAGAIVFTADEAEAAKEKGQKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPC
VSGAGSLRIDAAAQTLTVNGQVFKKGDHITIDGGTGQVLSGEVAMLQPELSGEFATLMGWADKVRKLKVRANAETPADAR
MARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRAALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFL
PHTAEETEEVAKSLGVDVERIERRNKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAIIAAKKTGEAVVPEIMVPL
IATKAEFDIVDAVIRKTADAVEKETGAKLTFQVGTMIELPRAALRAEEIAKGAEFFSFGTNDLTQTTFGVSRDDAGTFLG
PYVQKGIIEVDPFVSIDVDGVGELVKIGVERGRKSRPDIKLGICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAA
QAALKK
>Mature_885_residues
TKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTIPTEVCTYYYAHGQTYPAELKGDVEAALAQVGQLTG
KSFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDETVEAVARLAGDRRFAYDSYRRFITMYSDVVLGVSHHHFEEILE
HYKLQKGFNLDTELSADDWAALVVKYKEKVAAELGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAVN
VQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARKTAGSDKPSMELALPEAFKEFE
RISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKAALRIAVELAAEGLITEEEAIGRVDPAALDQLLHPAIDPKAE
RKVIATGLPASPGAAAGAIVFTADEAEAAKEKGQKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPCV
SGAGSLRIDAAAQTLTVNGQVFKKGDHITIDGGTGQVLSGEVAMLQPELSGEFATLMGWADKVRKLKVRANAETPADARM
ARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGRRAALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFLP
HTAEETEEVAKSLGVDVERIERRNKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAIIAAKKTGEAVVPEIMVPLI
ATKAEFDIVDAVIRKTADAVEKETGAKLTFQVGTMIELPRAALRAEEIAKGAEFFSFGTNDLTQTTFGVSRDDAGTFLGP
YVQKGIIEVDPFVSIDVDGVGELVKIGVERGRKSRPDIKLGICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAAQ
AALKK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=464, Percent_Identity=26.5086206896552, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787994, Length=744, Percent_Identity=26.2096774193548, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1789193, Length=419, Percent_Identity=24.1050119331742, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788726, Length=460, Percent_Identity=24.3478260869565, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 95636; Mature: 95505

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTIPTEVCTYYYAHGQT
CCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
YPAELKGDVEAALAQVGQLTGKSFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EAVARLAGDRRFAYDSYRRFITMYSDVVLGVSHHHFEEILEHYKLQKGFNLDTELSADDW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AALVVKYKEKVAAELGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEE
NVQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARK
EEHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHH
TAGSDKPSMELALPEAFKEFERISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKA
HCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHH
ALRIAVELAAEGLITEEEAIGRVDPAALDQLLHPAIDPKAERKVIATGLPASPGAAAGAI
HHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEE
VFTADEAEAAKEKGQKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCC
VSGAGSLRIDAAAQTLTVNGQVFKKGDHITIDGGTGQVLSGEVAMLQPELSGEFATLMGW
CCCCCCEEEECCEEEEEECCEEEECCCEEEEECCCCCEECCCEEEECCCCCCCHHHHHHH
ADKVRKLKVRANAETPADARMARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGR
HHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCH
RAALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFLPHTAEETEEVAKSLGVDVER
HHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
IERRNKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAIIAAKKTGEAVVPEIMVPL
HHHHCCHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IATKAEFDIVDAVIRKTADAVEKETGAKLTFQVGTMIELPRAALRAEEIAKGAEFFSFGT
HHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHCCCC
NDLTQTTFGVSRDDAGTFLGPYVQKGIIEVDPFVSIDVDGVGELVKIGVERGRKSRPDIK
CCHHHHHCCCCCCCCCCHHHHHHHCCCEEECCEEEEECCCHHHHHHHHHHHCCCCCCCEE
LGICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAAQAALKK
EEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKWVYTFGDGKAEGKADMKNLLGGKGANLAEMSNLGLPVPPGFTIPTEVCTYYYAHGQT
CCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
YPAELKGDVEAALAQVGQLTGKSFGDAANPLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EAVARLAGDRRFAYDSYRRFITMYSDVVLGVSHHHFEEILEHYKLQKGFNLDTELSADDW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AALVVKYKEKVAAELGKPFPQDPHEQLWGAIGAVFSSWMNARAITYRRLHSIPESWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEE
NVQSMVFGNMGETSATGVAFTRNPSTGENALYGEFLVNAQGEDVVAGIRTPQDLTEKARK
EEHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHH
TAGSDKPSMELALPEAFKEFERISHVLENHYRDMQDLEFTVEKGKLWMLQTRSGKRTAKA
HCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHH
ALRIAVELAAEGLITEEEAIGRVDPAALDQLLHPAIDPKAERKVIATGLPASPGAAAGAI
HHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEE
VFTADEAEAAKEKGQKVILVRVETSPEDIHGMHAAQGILTARGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHEECCCCCHHHHHHHHCCCCCC
VSGAGSLRIDAAAQTLTVNGQVFKKGDHITIDGGTGQVLSGEVAMLQPELSGEFATLMGW
CCCCCCEEEECCEEEEEECCEEEECCCEEEEECCCCCEECCCEEEECCCCCCCHHHHHHH
ADKVRKLKVRANAETPADARMARSFGAEGIGLSRTEHMFFDAGRILAVREMILADDEAGR
HHHHHEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCH
RAALAKLLPMQRDDFVELFEIMKGLPVTIRLLDPPLHEFLPHTAEETEEVAKSLGVDVER
HHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
IERRNKELHEVNPMLGFRGCRIAIAFPEIAEMQARAIFEAAIIAAKKTGEAVVPEIMVPL
HHHHCCHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IATKAEFDIVDAVIRKTADAVEKETGAKLTFQVGTMIELPRAALRAEEIAKGAEFFSFGT
HHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHCCCC
NDLTQTTFGVSRDDAGTFLGPYVQKGIIEVDPFVSIDVDGVGELVKIGVERGRKSRPDIK
CCHHHHHCCCCCCCCCCHHHHHHHCCCEEECCEEEEECCCHHHHHHHHHHHCCCCCCCEE
LGICGEHGGDPASVAFCHEVGLNYVSCSPFRVPIARLAAAQAALKK
EEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]