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The map label for this gene is gltD

Identifier: 154244433

GI number: 154244433

Start: 522260

End: 523681

Strand: Reverse

Name: gltD

Synonym: Xaut_0476

Alternate gene names: 154244433

Gene position: 523681-522260 (Counterclockwise)

Preceding gene: 154244434

Following gene: 154244430

Centisome position: 9.86

GC content: 68.0

Gene sequence:

>1422_bases
ATGGGCAAGGTCACGGGTTTTCTGGAGATCGACCGGCGGGAGCAGAAGTATCAGCCCGCTTCCGACCGCATCCGCCATTT
CCGCGAGTTCACGCTGCCATTGCCGGACGCGGAGGTGGCTAACCAGGCCTCGCGCTGCATGGATTGCGGCGTGCCCTTCT
GCCACGGGCCCAACGGCTGCCCCGTGCACAACCAGATTCCGGACTGGAACGACCTCGTCTATCGCGACGAGTGGGAGGAA
GCCGCGCGCAACCTCCACACCACGAACAACTTTCCCGAGTTCACCGGCCGCATCTGCCCCGCCCCGTGCGAGGAAGCCTG
CACCCTGAACCTCGAGGACATGCCCGTCACCATCAAGACGGTGGAGCAGGCCATCGCCGACAAGGCGTGGAAGAAGGGCT
GGATCAAGCCCGAGCCCGCCCTGGTGAAGACCGGCAAGAAGGTGGCCATCGTCGGCTCCGGCCCGGCGGGCCTTGCCGCC
GCCCAGCAGCTCGCCCGCGCCGGCCATGACGTGCATGTCTTTGAGCGCGAGCCCAAGGCCGGTGGCCTGCTGCGCTACGG
CATCCCCGACTTCAAGATGGAAAAGCACCACATCGACCGTCGCGTGAAGCAGATGGAAGCGGAAGGTGTGACGTTCCACT
ACAACGAGAACATCGGCATCGTGCGCCCGTTCCAGGAGCTGCTCGATACCCACGATGCGGTGCTCCTCGCCGGTGGCTCC
GAAGCCCCGCGCGATCCCAAGCTGCCGGGCCAGGAGATGGAGGGCGTGCACTTCGCCATGCCCTATCTCGTGCAGTCGAA
CCGCCGCGAAGGCGACGAGCCGGTCAACGAGATCCCGATCGTCGCCGGCGGCAAGCATGTGGTGGTGGTGGGCGGCGGCG
ACACCGCTTCCGACTGCGTGGGCACCGCCTTCCGGCAGGGGGCGCTCTCGGTCACCCAGCTCGACATCCGCGCCGTGCCT
CCCATGAAGGAGGACAAGCTGGCGGTGTGGCCCTATTGGCCCACCAAGTTTCGCACCTCCTCCTCCCAGGCCGAGGGCGC
CGAGCGTGAGTTCGCCTGCGCCACCCTGGGCATCGAGGGGCGCAACGGCCGCGTCACCGGCGTGAAGTGCGCCCGCGTGG
ACCAGAAGCGCCAGCCCATTCCCGGCACCGAGTTCGTGCTGCGCGCGGACCTGGTGTTCCTCGCCATCGGCTTTGCCCAT
GCGGCCCATCCCGGCCTCATCGAGCAGTCCGGCGTGGTGCTGGACAAGCGCGGCAACGTGCAGGCCGACGATCTCGCCTA
TGCCACGTCCGTGCCCAAGCTGTTCGCGGCGGGCGACATGCGGCGCGGCCAGTCGCTGGTGGTGTGGGCCATCCGGGAAG
GCCGCCAGGCCGCCCACGCCATCGACAAGGAACTCATGGGCGCGACCACCCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
CAAGCTGGAACAATTCTTGTATTTGGACACGTGGTGCGGCACCCGGATCGATGGGGTGCGCGCGGCGGTTCCCTCAACGA
GGTTCGCCTAAGGGGTTCTG

Downstream 100 bases:

>100_bases
GCCGGCAGGGGACGGAGTGATCCCTCAGGACCGCTCACGCTATCCCCGCACACGGGGCCGAAATCGGCACCGTGTGCGGG
CGGGTCTTCAGAACTTATAA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MGKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVANQASRCMDCGVPFCHGPNGCPVHNQIPDWNDLVYRDEWEE
AARNLHTTNNFPEFTGRICPAPCEEACTLNLEDMPVTIKTVEQAIADKAWKKGWIKPEPALVKTGKKVAIVGSGPAGLAA
AQQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGIVRPFQELLDTHDAVLLAGGS
EAPRDPKLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPIVAGGKHVVVVGGGDTASDCVGTAFRQGALSVTQLDIRAVP
PMKEDKLAVWPYWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDQKRQPIPGTEFVLRADLVFLAIGFAH
AAHPGLIEQSGVVLDKRGNVQADDLAYATSVPKLFAAGDMRRGQSLVVWAIREGRQAAHAIDKELMGATTLPR

Sequences:

>Translated_473_residues
MGKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVANQASRCMDCGVPFCHGPNGCPVHNQIPDWNDLVYRDEWEE
AARNLHTTNNFPEFTGRICPAPCEEACTLNLEDMPVTIKTVEQAIADKAWKKGWIKPEPALVKTGKKVAIVGSGPAGLAA
AQQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGIVRPFQELLDTHDAVLLAGGS
EAPRDPKLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPIVAGGKHVVVVGGGDTASDCVGTAFRQGALSVTQLDIRAVP
PMKEDKLAVWPYWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDQKRQPIPGTEFVLRADLVFLAIGFAH
AAHPGLIEQSGVVLDKRGNVQADDLAYATSVPKLFAAGDMRRGQSLVVWAIREGRQAAHAIDKELMGATTLPR
>Mature_472_residues
GKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVANQASRCMDCGVPFCHGPNGCPVHNQIPDWNDLVYRDEWEEA
ARNLHTTNNFPEFTGRICPAPCEEACTLNLEDMPVTIKTVEQAIADKAWKKGWIKPEPALVKTGKKVAIVGSGPAGLAAA
QQLARAGHDVHVFEREPKAGGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGIVRPFQELLDTHDAVLLAGGSE
APRDPKLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPIVAGGKHVVVVGGGDTASDCVGTAFRQGALSVTQLDIRAVPP
MKEDKLAVWPYWPTKFRTSSSQAEGAEREFACATLGIEGRNGRVTGVKCARVDQKRQPIPGTEFVLRADLVFLAIGFAHA
AHPGLIEQSGVVLDKRGNVQADDLAYATSVPKLFAAGDMRRGQSLVVWAIREGRQAAHAIDKELMGATTLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=23.8512035010941, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1789606, Length=476, Percent_Identity=38.8655462184874, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1788811, Length=453, Percent_Identity=39.2935982339956, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=37.5, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=28.5077951002227, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1789243, Length=423, Percent_Identity=30.4964539007092, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=42.3625254582485, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984108, Length=449, Percent_Identity=24.4988864142539, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=35.0574712643678, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=489, Percent_Identity=42.5357873210634, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=46.4876033057851, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=46.4876033057851, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=46.4876033057851, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=46.4876033057851, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640763, Length=456, Percent_Identity=24.780701754386, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18858217, Length=456, Percent_Identity=24.780701754386, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17137178, Length=157, Percent_Identity=32.484076433121, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51937; Mature: 51805

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVANQASRCMDCGVPFCHGPNGC
CCCEEEEEEECCHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYRDEWEEAARNLHTTNNFPEFTGRICPAPCEEACTLNLEDMPVTIKT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHEECCCCCCCHHHH
VEQAIADKAWKKGWIKPEPALVKTGKKVAIVGSGPAGLAAAQQLARAGHDVHVFEREPKA
HHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCC
GGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGIVRPFQELLDTHDAVLLAGGS
CCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCEECHHHHHHCCCCEEEEECCC
EAPRDPKLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPIVAGGKHVVVVGGGDTASDCV
CCCCCCCCCCHHCCCEEEEHHHHHHCCCCCCCCCHHHCCEEECCCEEEEEECCCCHHHHH
GTAFRQGALSVTQLDIRAVPPMKEDKLAVWPYWPTKFRTSSSQAEGAEREFACATLGIEG
HHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEC
RNGRVTGVKCARVDQKRQPIPGTEFVLRADLVFLAIGFAHAAHPGLIEQSGVVLDKRGNV
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
QADDLAYATSVPKLFAAGDMRRGQSLVVWAIREGRQAAHAIDKELMGATTLPR
CHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKVTGFLEIDRREQKYQPASDRIRHFREFTLPLPDAEVANQASRCMDCGVPFCHGPNGC
CCEEEEEEECCHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYRDEWEEAARNLHTTNNFPEFTGRICPAPCEEACTLNLEDMPVTIKT
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHEECCCCCCCHHHH
VEQAIADKAWKKGWIKPEPALVKTGKKVAIVGSGPAGLAAAQQLARAGHDVHVFEREPKA
HHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCC
GGLLRYGIPDFKMEKHHIDRRVKQMEAEGVTFHYNENIGIVRPFQELLDTHDAVLLAGGS
CCEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCEECHHHHHHCCCCEEEEECCC
EAPRDPKLPGQEMEGVHFAMPYLVQSNRREGDEPVNEIPIVAGGKHVVVVGGGDTASDCV
CCCCCCCCCCHHCCCEEEEHHHHHHCCCCCCCCCHHHCCEEECCCEEEEEECCCCHHHHH
GTAFRQGALSVTQLDIRAVPPMKEDKLAVWPYWPTKFRTSSSQAEGAEREFACATLGIEG
HHHHHHCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEC
RNGRVTGVKCARVDQKRQPIPGTEFVLRADLVFLAIGFAHAAHPGLIEQSGVVLDKRGNV
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
QADDLAYATSVPKLFAAGDMRRGQSLVVWAIREGRQAAHAIDKELMGATTLPR
CHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]