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The map label for this gene is fixI [H]

Identifier: 154244422

GI number: 154244422

Start: 508352

End: 510583

Strand: Direct

Name: fixI [H]

Synonym: Xaut_0465

Alternate gene names: 154244422

Gene position: 508352-510583 (Clockwise)

Preceding gene: 154244421

Following gene: 154244423

Centisome position: 9.58

GC content: 72.89

Gene sequence:

>2232_bases
ATGGCTGAGGCGATCGACTATTCCGTTTTCGTCGCGCGGTCCGAGGACGGCCTCGCCCAGATGAGCCTCGCCGTGGACGG
CATCGACTGCGCCGCCTGCATCGCCGAGATCGAGGACGGGGTAAAGGCGCTGCCTGGCGTGGTGCGCGCGCGCCTCAATT
ATTCCACCCACCGCCTCACCGTCGCCTGGCGCGAGGAGACCTCACCGGACCGGGTGGTGAGCCAGCTGGAAGCCCTGGGC
TATCGCGCCCACCCCTTCGCCGGCCGAGCCGAGGAGAGCGAGGCTCGCCACGCCCGCTGGCTGCTGCGCTGCCTGGGCGT
CGCCGGCTTCGCGGCCATGAACATCATGCTGCTGTCGGTGTCGGTGTGGTCGGGCAATGTCACCGACATCACGCCGGAGA
CGCGGGACTTCTTCCATTGGGTCTCGGCGCTCATCGCGTTGCCGGCGGCCGCCTATGCCGGCCAGCCCTTCTTCCAGAGC
GCGTTCCGGGCGGTGCGCTCGGGCCGGCTCAACATGGACGTGCCCATCACCATCGGCGTCACCCTGGCGCTGGGCATGAG
CGTGCTGGAGACGGCGCTGAGCCAGCCCCACGCCTATTTCGACAGCGCCATCATGCTGCTGTTCTTCCTTTTGGCCGGGC
GCTACCTCGACCACGAGGCGCGCCGGCGCACCCGCGCCACGGCCGGCAACATCGCCGCCCTGCGGGGCGAGATGGCCCAT
CGCCTCGGCACCGACGGCGTGCCGGTGCTGGTGCCGGTGCAGGCACTGCAGCCGGGCGACACCATCCTCGTCGCGCCCGG
TGAGCGGGTGGCGGCGGATGGCGTCATCGCTTCCGGCCAGTCCAGCATCGACGAAAGCCTCGTCACCGGCGAGACCCTGC
CGCGCGCCCTGAAGGCGGGGGATGCGGTCTACGCCGGCAGCCTCAACGGCGAGGGCGCGTTGCACCTGACGGTTACCGCC
GGCGGCGAGAACACGCTGGTGGACGATGTGCAGCGCCTGCTGGATGGCGCGCTGGCCGAGAAGGGCGCCTATGTGCGCCT
CGCCGACCGGGTGGCGCGGCTCTACGCCCCCATGGTGCATCTGACGGCGCTGGTCTCCGCCATCGGCTGGCTCCTCGCCG
GAGCGGACCTGCATCATGCGGTGATGATCGCGGTGGCCGTGCTCATCATCACCTGTCCCTGCGCGCTGGCCCTCGCCGTG
CCCGCGGTGCAGGTGACGGCCACCGGCCGGCTGTTCCGCGCCGGCCTTCTCATCAATGCCGGCGACATGCTGGAGCGTCT
CGCCGCCGTGGACACGGTGGTGTTCGACAAGACCGGCACCCTGACGCTGCCGGAGCCCGCCCTTGCCCTGCCGCGCGGGG
CGGACCGGGCGCTGGTGGGCCTTGCCGGGCGGCTGGCCCTGTCGAGCCGCCACCCGCTGGCCCGGGCCCTCGCCCCGCGC
GCGGACGGGCCGGCCATCGAGGGCGTGACCGAGGTGAGCGGGCAGGGCGTGGAGGCGCTGGTGGACGGCGCGGTCGTGCG
CCTCGGCAGCCTCGCCTTCTGCGGCGCGGCGCCGCCCGCCGATCTGCCGGCCGGCGCCTCCCTCATCGCCCTGCGTCGTG
GCGGCCAGACCCTGGTCATGGGTGTCGAACAGGCCCTGCGGCCGGACGCGCCGGAGGTGGTGGCGGCGCTGAAGGCGCGC
GGCCTCGACGTGCGCATCCTCTCCGGCGACCGGGAGGCCGCCGTGACCCCCGTGGCGCAGGCGCTGGGCATCGCCCATTG
GGACGCAGGCCTCAAGCCCGCCGACAAGATCGCCGCCCTTGATCACCTGAAGGCGGAAGGCCGGCGCGTGCTGATGGTGG
GCGATGGCCTCAACGATGCTCCGGCGCTGGCCGCCGCCACGGTTTCCCTCTCCCCCGCCACCGGCGCGGCGGTAACGCAG
GCCCATGCGGACGCGGTGTTCCTCGGCCGCCGCCTCGCGCCGGTGATGGCGGCGGTGGACGGGGCGGGGGTCGCGGCGCG
GCTGATGCGGCAGAACCTCGCCATCGCCGTGCTCTACAATGTGATCGCGGTGCCGCTGGCCATCGCCGGCTTTGTGACGC
CGCTGGTGGCAGCGCTTGCCATGTCCGGCTCTTCCATACTCGTGACCGTCAACGCCTTGCGCGCGGCGCGGCCGGGGCGC
AAGGATGAGGCGACCGACGCCGCGGCCCCTGCCGGCGGCGATGCCCGCACATTGGAAGAGGCGGCCGCGTGA

Upstream 100 bases:

>100_bases
TTCGTCGCGCCGGCCGGCCTGTCGTACTTTTCTCTCATTCGGGTCCGCATCTTCCCGCCGCGCGGTGCGGCGGGGCCCCG
CAGCCCCCGGGGATGGAGCC

Downstream 100 bases:

>100_bases
ACGTCCTCGTCTATCTCCTGCCGCTCGCGCTGCTGCTCGGCCTCACCGGCCTCTTCGCCTTCCTGTGGAGCCTGAAGAGC
GGCCAGTATGACGATCTCGA

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MAEAIDYSVFVARSEDGLAQMSLAVDGIDCAACIAEIEDGVKALPGVVRARLNYSTHRLTVAWREETSPDRVVSQLEALG
YRAHPFAGRAEESEARHARWLLRCLGVAGFAAMNIMLLSVSVWSGNVTDITPETRDFFHWVSALIALPAAAYAGQPFFQS
AFRAVRSGRLNMDVPITIGVTLALGMSVLETALSQPHAYFDSAIMLLFFLLAGRYLDHEARRRTRATAGNIAALRGEMAH
RLGTDGVPVLVPVQALQPGDTILVAPGERVAADGVIASGQSSIDESLVTGETLPRALKAGDAVYAGSLNGEGALHLTVTA
GGENTLVDDVQRLLDGALAEKGAYVRLADRVARLYAPMVHLTALVSAIGWLLAGADLHHAVMIAVAVLIITCPCALALAV
PAVQVTATGRLFRAGLLINAGDMLERLAAVDTVVFDKTGTLTLPEPALALPRGADRALVGLAGRLALSSRHPLARALAPR
ADGPAIEGVTEVSGQGVEALVDGAVVRLGSLAFCGAAPPADLPAGASLIALRRGGQTLVMGVEQALRPDAPEVVAALKAR
GLDVRILSGDREAAVTPVAQALGIAHWDAGLKPADKIAALDHLKAEGRRVLMVGDGLNDAPALAAATVSLSPATGAAVTQ
AHADAVFLGRRLAPVMAAVDGAGVAARLMRQNLAIAVLYNVIAVPLAIAGFVTPLVAALAMSGSSILVTVNALRAARPGR
KDEATDAAAPAGGDARTLEEAAA

Sequences:

>Translated_743_residues
MAEAIDYSVFVARSEDGLAQMSLAVDGIDCAACIAEIEDGVKALPGVVRARLNYSTHRLTVAWREETSPDRVVSQLEALG
YRAHPFAGRAEESEARHARWLLRCLGVAGFAAMNIMLLSVSVWSGNVTDITPETRDFFHWVSALIALPAAAYAGQPFFQS
AFRAVRSGRLNMDVPITIGVTLALGMSVLETALSQPHAYFDSAIMLLFFLLAGRYLDHEARRRTRATAGNIAALRGEMAH
RLGTDGVPVLVPVQALQPGDTILVAPGERVAADGVIASGQSSIDESLVTGETLPRALKAGDAVYAGSLNGEGALHLTVTA
GGENTLVDDVQRLLDGALAEKGAYVRLADRVARLYAPMVHLTALVSAIGWLLAGADLHHAVMIAVAVLIITCPCALALAV
PAVQVTATGRLFRAGLLINAGDMLERLAAVDTVVFDKTGTLTLPEPALALPRGADRALVGLAGRLALSSRHPLARALAPR
ADGPAIEGVTEVSGQGVEALVDGAVVRLGSLAFCGAAPPADLPAGASLIALRRGGQTLVMGVEQALRPDAPEVVAALKAR
GLDVRILSGDREAAVTPVAQALGIAHWDAGLKPADKIAALDHLKAEGRRVLMVGDGLNDAPALAAATVSLSPATGAAVTQ
AHADAVFLGRRLAPVMAAVDGAGVAARLMRQNLAIAVLYNVIAVPLAIAGFVTPLVAALAMSGSSILVTVNALRAARPGR
KDEATDAAAPAGGDARTLEEAAA
>Mature_742_residues
AEAIDYSVFVARSEDGLAQMSLAVDGIDCAACIAEIEDGVKALPGVVRARLNYSTHRLTVAWREETSPDRVVSQLEALGY
RAHPFAGRAEESEARHARWLLRCLGVAGFAAMNIMLLSVSVWSGNVTDITPETRDFFHWVSALIALPAAAYAGQPFFQSA
FRAVRSGRLNMDVPITIGVTLALGMSVLETALSQPHAYFDSAIMLLFFLLAGRYLDHEARRRTRATAGNIAALRGEMAHR
LGTDGVPVLVPVQALQPGDTILVAPGERVAADGVIASGQSSIDESLVTGETLPRALKAGDAVYAGSLNGEGALHLTVTAG
GENTLVDDVQRLLDGALAEKGAYVRLADRVARLYAPMVHLTALVSAIGWLLAGADLHHAVMIAVAVLIITCPCALALAVP
AVQVTATGRLFRAGLLINAGDMLERLAAVDTVVFDKTGTLTLPEPALALPRGADRALVGLAGRLALSSRHPLARALAPRA
DGPAIEGVTEVSGQGVEALVDGAVVRLGSLAFCGAAPPADLPAGASLIALRRGGQTLVMGVEQALRPDAPEVVAALKARG
LDVRILSGDREAAVTPVAQALGIAHWDAGLKPADKIAALDHLKAEGRRVLMVGDGLNDAPALAAATVSLSPATGAAVTQA
HADAVFLGRRLAPVMAAVDGAGVAARLMRQNLAIAVLYNVIAVPLAIAGFVTPLVAALAMSGSSILVTVNALRAARPGRK
DEATDAAAPAGGDARTLEEAAA

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=790, Percent_Identity=26.3291139240506, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI115529486, Length=539, Percent_Identity=26.3450834879406, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI55743073, Length=521, Percent_Identity=28.4069097888676, Blast_Score=174, Evalue=3e-43,
Organism=Escherichia coli, GI1786691, Length=763, Percent_Identity=30.9305373525557, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1789879, Length=721, Percent_Identity=30.374479889043, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786914, Length=523, Percent_Identity=26.0038240917782, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556548, Length=776, Percent_Identity=26.6752577319588, Blast_Score=209, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319772, Length=611, Percent_Identity=27.986906710311, Blast_Score=210, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6320475, Length=753, Percent_Identity=24.4355909694555, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321430, Length=209, Percent_Identity=25.8373205741627, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325221, Length=209, Percent_Identity=25.8373205741627, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221329854, Length=523, Percent_Identity=31.1663479923518, Blast_Score=205, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 76765; Mature: 76634

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAIDYSVFVARSEDGLAQMSLAVDGIDCAACIAEIEDGVKALPGVVRARLNYSTHRLT
CCCCEEEEEEEEECCCCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VAWREETSPDRVVSQLEALGYRAHPFAGRAEESEARHARWLLRCLGVAGFAAMNIMLLSV
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SVWSGNVTDITPETRDFFHWVSALIALPAAAYAGQPFFQSAFRAVRSGRLNMDVPITIGV
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEHH
TLALGMSVLETALSQPHAYFDSAIMLLFFLLAGRYLDHEARRRTRATAGNIAALRGEMAH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCHHHHHHHHHCCCCHHHHHHHHHH
RLGTDGVPVLVPVQALQPGDTILVAPGERVAADGVIASGQSSIDESLVTGETLPRALKAG
HCCCCCCCEEEEEECCCCCCEEEECCCCCEECCCEEECCCHHHHHHHHCCHHHHHHHHCC
DAVYAGSLNGEGALHLTVTAGGENTLVDDVQRLLDGALAEKGAYVRLADRVARLYAPMVH
CEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
LTALVSAIGWLLAGADLHHAVMIAVAVLIITCPCALALAVPAVQVTATGRLFRAGLLINA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCEEECH
GDMLERLAAVDTVVFDKTGTLTLPEPALALPRGADRALVGLAGRLALSSRHPLARALAPR
HHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCC
ADGPAIEGVTEVSGQGVEALVDGAVVRLGSLAFCGAAPPADLPAGASLIALRRGGQTLVM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEECCCCEEEE
GVEQALRPDAPEVVAALKARGLDVRILSGDREAAVTPVAQALGIAHWDAGLKPADKIAAL
EHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DHLKAEGRRVLMVGDGLNDAPALAAATVSLSPATGAAVTQAHADAVFLGRRLAPVMAAVD
HHHHCCCCEEEEEECCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GAGVAARLMRQNLAIAVLYNVIAVPLAIAGFVTPLVAALAMSGSSILVTVNALRAARPGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCC
KDEATDAAAPAGGDARTLEEAAA
CCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AEAIDYSVFVARSEDGLAQMSLAVDGIDCAACIAEIEDGVKALPGVVRARLNYSTHRLT
CCCEEEEEEEEECCCCCHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
VAWREETSPDRVVSQLEALGYRAHPFAGRAEESEARHARWLLRCLGVAGFAAMNIMLLSV
EEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
SVWSGNVTDITPETRDFFHWVSALIALPAAAYAGQPFFQSAFRAVRSGRLNMDVPITIGV
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEHH
TLALGMSVLETALSQPHAYFDSAIMLLFFLLAGRYLDHEARRRTRATAGNIAALRGEMAH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCHHHHHHHHHCCCCHHHHHHHHHH
RLGTDGVPVLVPVQALQPGDTILVAPGERVAADGVIASGQSSIDESLVTGETLPRALKAG
HCCCCCCCEEEEEECCCCCCEEEECCCCCEECCCEEECCCHHHHHHHHCCHHHHHHHHCC
DAVYAGSLNGEGALHLTVTAGGENTLVDDVQRLLDGALAEKGAYVRLADRVARLYAPMVH
CEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
LTALVSAIGWLLAGADLHHAVMIAVAVLIITCPCALALAVPAVQVTATGRLFRAGLLINA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCEEECH
GDMLERLAAVDTVVFDKTGTLTLPEPALALPRGADRALVGLAGRLALSSRHPLARALAPR
HHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCC
ADGPAIEGVTEVSGQGVEALVDGAVVRLGSLAFCGAAPPADLPAGASLIALRRGGQTLVM
CCCCCCCCHHHCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEEECCCCEEEE
GVEQALRPDAPEVVAALKARGLDVRILSGDREAAVTPVAQALGIAHWDAGLKPADKIAAL
EHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DHLKAEGRRVLMVGDGLNDAPALAAATVSLSPATGAAVTQAHADAVFLGRRLAPVMAAVD
HHHHCCCCEEEEEECCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GAGVAARLMRQNLAIAVLYNVIAVPLAIAGFVTPLVAALAMSGSSILVTVNALRAARPGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCC
KDEATDAAAPAGGDARTLEEAAA
CCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]