| Definition | Xanthobacter autotrophicus Py2 chromosome, complete genome. |
|---|---|
| Accession | NC_009720 |
| Length | 5,308,934 |
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The map label for this gene is ecaIM [H]
Identifier: 154244393
GI number: 154244393
Start: 477076
End: 479166
Strand: Reverse
Name: ecaIM [H]
Synonym: Xaut_0436
Alternate gene names: 154244393
Gene position: 479166-477076 (Counterclockwise)
Preceding gene: 154244394
Following gene: 154244392
Centisome position: 9.03
GC content: 59.11
Gene sequence:
>2091_bases TTGAAAAGTCTGCTGGATCAGCTTCCCGCCATCGTCGCGGATGGCAAGAAGGAAGCCGAGCGCGTGATGGAGCGGGCCGA GAGCAACTATCGCCTCGGGCTGCAAACGCGCGAATTGGTTGTCCCCTCGAAGGATAGCAAGTGGCAGGATTTGTTCCGCG AGAAGCGCGGAATCGCCGGCGACGTTAACTTGGATGAGGGGAGCGCCCTGATATACGGCGACAACCTCTTGGCAATGGCC GCGCTGATTGCCGGCGGCTCTCGAAACGAGACGCTACGCAATAAGATTGACTTCATTTACATTGATCCACCGTTCGATAG TAAAGCTGACTATCGAACAAAGATAGTCCTACCGGATTCTGAAATCGAGCAAAACCCAAGCATCTTGGAGCAATTTGCAT ACTCTGACACTTGGGCAGACGGAACGGCGTCATATTTGCAAATGATTACGCCTCGCCTCTCTCTTATGAGGGAGCTGCTA TCGGACGTTGGCAGCATATACATCCACATTGATCGCAGCGTCGGGCCCTACGTCAAGGTCATCGCCGACGAAATTTTCGG CCGCTACAACTTCGAGAACGAGATTATCTGGCGGCGCACCACGTCGAGGGGCGGCAGCGAGTATTATAACCACGTCCACG ACAACATACTTTTCTACACTAAAAACGGCTGCGGGATTTGGAATCAGCAATATACTCCCTACACTGATGGTTATATCGAC GGCATGTTCCGTAAGGTAGATGATAGAGGCCGGCGTTATCGTGAAAGCCCCCTTACTGCGCCGGGGCGGCGAGACGGCGA TTCCGGGCGACCTTGGAAGGGTGTCGATCCCAATCAGATGGGAAAAGGCCGCCATTGGGCCGTTCCGGGTTTTCTGCGCC ACCTGATTTCGGCCGAGGCGCAATGCAGCGTTCAACGGTCTCTCGATGAACTCGAAGTGCAAGGGCGGATCGTCTGGGCG AAGGACGGTGCCGGCCGCCCCAACGTCGTGCAATATGTGGACGATCTGCCGGGCGTCGAGCTTCAATCTATGTGGACGGA TATCGGTCTCGAAGCCGGCCCCGAATATGACACTCGAAAGCCGGAACGGCTCATCGAGCGGCTCATAGTGTCGTCAACGA ACGCGGATTCGATCGTCGCTGATTTCTTCGTCGGCTCTGGCACGACGGCCGCCGTCGCCGAGCGGCTGGGGCGTCGCTGG ATTGCCAGCGATCTCGGCAAGCCTGCCTGCATGATTACGCGCAAGCGCCTGATCGACCAAGATGCCAAGCCCTTCCTCTA CCAGCATATCGGCGACTATCAGGTTGAACAGCTCCGCTCGACGATGGGCGCAAAGTTCCGCATCGGCGACCTCGCTGAAA TTGTCCTCGGCCTCTACGGCGCGCTGCCGCTGCCAATCGAGGAAAACCCGAACAAGAACATGGGCCGGCTTCAGGGGAGC AAGACGCTCGTCCTCGCCGACAGCCCGAACAAGATGACCGGCCTCGCCACGCTGCGCCGCGCGATCGAAATCCGCGACAA CCTCATGGGCGGCTGGGACAAGGTTGTAATTCTCGGCTGGAACTTCTCGCCGATGATCGGCCACGACATCGAGGCGCTGG GGCAGGGAGACCGCCTCGAAGTGCTGGTGATCCCGCCGGACCTGCTCGACCGGCTCAAGAAGAAGGGCCACAAGCTCAAG GCCGACGAAGTGCGCTTCGCTTCGCTCCAGTATCTCAAGCTCGGCGCGGTGACGCGCAAGCAAGGAGCCAAGGACGAATC GCTGTCGGTTGAGATCGCCAACTACGTGCTGCTGTCACCGGAGGCGCTGAATCTCGATGAGGCGAACCGCGACAAGCTGC AAAAGGTAGTCAATGCCGACCCTTTGGCGCTGATCGAATACTGGAGCGTCGATCCCGACTATGACGGCGAGGTGTTCCGC TCCGTCTGGCAGGATTACCGGGGCAACACGGACAATGATGGCGATCCGTACCGCGTCGGCCGCACGGCCCGGCTAGATGG TCTGCCGAAGAAGGACGGCGCGCGCCGTGTCTGCGTCCGCGTGGTCGATGTGTTCGGCTTCGAGGCCGAAGCGACGGCCG AGGTGGCGTGA
Upstream 100 bases:
>100_bases GTTGAAGAATACGAGCGGCTGAAGGCGGCGCATCAGCCCGTACCATCGCCCGAGCACAACACAGGGCCAAAAGCCTGATG GACCTTTGGGGGATACTGAA
Downstream 100 bases:
>100_bases TGGCGAGCATCAACGATCTTTCGCTGGCCCGTGGACTGACGGCCAAGGTCGAAGAGGCCTGCGCCGGGCTTGAAAATGGC GAGGCCCCGATCCTCGGCCA
Product: DNA methylase N-4/N-6 domain-containing protein
Products: NA
Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]
Number of amino acids: Translated: 696; Mature: 696
Protein sequence:
>696_residues MKSLLDQLPAIVADGKKEAERVMERAESNYRLGLQTRELVVPSKDSKWQDLFREKRGIAGDVNLDEGSALIYGDNLLAMA ALIAGGSRNETLRNKIDFIYIDPPFDSKADYRTKIVLPDSEIEQNPSILEQFAYSDTWADGTASYLQMITPRLSLMRELL SDVGSIYIHIDRSVGPYVKVIADEIFGRYNFENEIIWRRTTSRGGSEYYNHVHDNILFYTKNGCGIWNQQYTPYTDGYID GMFRKVDDRGRRYRESPLTAPGRRDGDSGRPWKGVDPNQMGKGRHWAVPGFLRHLISAEAQCSVQRSLDELEVQGRIVWA KDGAGRPNVVQYVDDLPGVELQSMWTDIGLEAGPEYDTRKPERLIERLIVSSTNADSIVADFFVGSGTTAAVAERLGRRW IASDLGKPACMITRKRLIDQDAKPFLYQHIGDYQVEQLRSTMGAKFRIGDLAEIVLGLYGALPLPIEENPNKNMGRLQGS KTLVLADSPNKMTGLATLRRAIEIRDNLMGGWDKVVILGWNFSPMIGHDIEALGQGDRLEVLVIPPDLLDRLKKKGHKLK ADEVRFASLQYLKLGAVTRKQGAKDESLSVEIANYVLLSPEALNLDEANRDKLQKVVNADPLALIEYWSVDPDYDGEVFR SVWQDYRGNTDNDGDPYRVGRTARLDGLPKKDGARRVCVRVVDVFGFEAEATAEVA
Sequences:
>Translated_696_residues MKSLLDQLPAIVADGKKEAERVMERAESNYRLGLQTRELVVPSKDSKWQDLFREKRGIAGDVNLDEGSALIYGDNLLAMA ALIAGGSRNETLRNKIDFIYIDPPFDSKADYRTKIVLPDSEIEQNPSILEQFAYSDTWADGTASYLQMITPRLSLMRELL SDVGSIYIHIDRSVGPYVKVIADEIFGRYNFENEIIWRRTTSRGGSEYYNHVHDNILFYTKNGCGIWNQQYTPYTDGYID GMFRKVDDRGRRYRESPLTAPGRRDGDSGRPWKGVDPNQMGKGRHWAVPGFLRHLISAEAQCSVQRSLDELEVQGRIVWA KDGAGRPNVVQYVDDLPGVELQSMWTDIGLEAGPEYDTRKPERLIERLIVSSTNADSIVADFFVGSGTTAAVAERLGRRW IASDLGKPACMITRKRLIDQDAKPFLYQHIGDYQVEQLRSTMGAKFRIGDLAEIVLGLYGALPLPIEENPNKNMGRLQGS KTLVLADSPNKMTGLATLRRAIEIRDNLMGGWDKVVILGWNFSPMIGHDIEALGQGDRLEVLVIPPDLLDRLKKKGHKLK ADEVRFASLQYLKLGAVTRKQGAKDESLSVEIANYVLLSPEALNLDEANRDKLQKVVNADPLALIEYWSVDPDYDGEVFR SVWQDYRGNTDNDGDPYRVGRTARLDGLPKKDGARRVCVRVVDVFGFEAEATAEVA >Mature_696_residues MKSLLDQLPAIVADGKKEAERVMERAESNYRLGLQTRELVVPSKDSKWQDLFREKRGIAGDVNLDEGSALIYGDNLLAMA ALIAGGSRNETLRNKIDFIYIDPPFDSKADYRTKIVLPDSEIEQNPSILEQFAYSDTWADGTASYLQMITPRLSLMRELL SDVGSIYIHIDRSVGPYVKVIADEIFGRYNFENEIIWRRTTSRGGSEYYNHVHDNILFYTKNGCGIWNQQYTPYTDGYID GMFRKVDDRGRRYRESPLTAPGRRDGDSGRPWKGVDPNQMGKGRHWAVPGFLRHLISAEAQCSVQRSLDELEVQGRIVWA KDGAGRPNVVQYVDDLPGVELQSMWTDIGLEAGPEYDTRKPERLIERLIVSSTNADSIVADFFVGSGTTAAVAERLGRRW IASDLGKPACMITRKRLIDQDAKPFLYQHIGDYQVEQLRSTMGAKFRIGDLAEIVLGLYGALPLPIEENPNKNMGRLQGS KTLVLADSPNKMTGLATLRRAIEIRDNLMGGWDKVVILGWNFSPMIGHDIEALGQGDRLEVLVIPPDLLDRLKKKGHKLK ADEVRFASLQYLKLGAVTRKQGAKDESLSVEIANYVLLSPEALNLDEANRDKLQKVVNADPLALIEYWSVDPDYDGEVFR SVWQDYRGNTDNDGDPYRVGRTARLDGLPKKDGARRVCVRVVDVFGFEAEATAEVA
Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]
COG id: COG2189
COG function: function code L; Adenine specific DNA methylase Mod
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002295 - InterPro: IPR002941 - InterPro: IPR002052 [H]
Pfam domain/function: PF01555 N6_N4_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 78186; Mature: 78186
Theoretical pI: Translated: 5.43; Mature: 5.43
Prosite motif: PS00092 N6_MTASE ; PS00216 SUGAR_TRANSPORT_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSLLDQLPAIVADGKKEAERVMERAESNYRLGLQTRELVVPSKDSKWQDLFREKRGIAG CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHHHHHCCCCC DVNLDEGSALIYGDNLLAMAALIAGGSRNETLRNKIDFIYIDPPFDSKADYRTKIVLPDS CCCCCCCCEEEECCHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCEEEEEECCC EIEQNPSILEQFAYSDTWADGTASYLQMITPRLSLMRELLSDVGSIYIHIDRSVGPYVKV HHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH IADEIFGRYNFENEIIWRRTTSRGGSEYYNHVHDNILFYTKNGCGIWNQQYTPYTDGYID HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHH GMFRKVDDRGRRYRESPLTAPGRRDGDSGRPWKGVDPNQMGKGRHWAVPGFLRHLISAEA HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHCCHH QCSVQRSLDELEVQGRIVWAKDGAGRPNVVQYVDDLPGVELQSMWTDIGLEAGPEYDTRK HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCC PERLIERLIVSSTNADSIVADFFVGSGTTAAVAERLGRRWIASDLGKPACMITRKRLIDQ HHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC DAKPFLYQHIGDYQVEQLRSTMGAKFRIGDLAEIVLGLYGALPLPIEENPNKNMGRLQGS CCCHHHHHHCCCHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC KTLVLADSPNKMTGLATLRRAIEIRDNLMGGWDKVVILGWNFSPMIGHDIEALGQGDRLE CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEE VLVIPPDLLDRLKKKGHKLKADEVRFASLQYLKLGAVTRKQGAKDESLSVEIANYVLLSP EEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEEECC EALNLDEANRDKLQKVVNADPLALIEYWSVDPDYDGEVFRSVWQDYRGNTDNDGDPYRVG CCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEECC RTARLDGLPKKDGARRVCVRVVDVFGFEAEATAEVA CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC >Mature Secondary Structure MKSLLDQLPAIVADGKKEAERVMERAESNYRLGLQTRELVVPSKDSKWQDLFREKRGIAG CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHHHHHCCCCC DVNLDEGSALIYGDNLLAMAALIAGGSRNETLRNKIDFIYIDPPFDSKADYRTKIVLPDS CCCCCCCCEEEECCHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCEEEEEECCC EIEQNPSILEQFAYSDTWADGTASYLQMITPRLSLMRELLSDVGSIYIHIDRSVGPYVKV HHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHH IADEIFGRYNFENEIIWRRTTSRGGSEYYNHVHDNILFYTKNGCGIWNQQYTPYTDGYID HHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHH GMFRKVDDRGRRYRESPLTAPGRRDGDSGRPWKGVDPNQMGKGRHWAVPGFLRHLISAEA HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHCCHH QCSVQRSLDELEVQGRIVWAKDGAGRPNVVQYVDDLPGVELQSMWTDIGLEAGPEYDTRK HHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCC PERLIERLIVSSTNADSIVADFFVGSGTTAAVAERLGRRWIASDLGKPACMITRKRLIDQ HHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC DAKPFLYQHIGDYQVEQLRSTMGAKFRIGDLAEIVLGLYGALPLPIEENPNKNMGRLQGS CCCHHHHHHCCCHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCC KTLVLADSPNKMTGLATLRRAIEIRDNLMGGWDKVVILGWNFSPMIGHDIEALGQGDRLE CEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEE VLVIPPDLLDRLKKKGHKLKADEVRFASLQYLKLGAVTRKQGAKDESLSVEIANYVLLSP EEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEEECC EALNLDEANRDKLQKVVNADPLALIEYWSVDPDYDGEVFRSVWQDYRGNTDNDGDPYRVG CCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEECC RTARLDGLPKKDGARRVCVRVVDVFGFEAEATAEVA CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2183182 [H]