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The map label for this gene is rlmN

Identifier: 154244355

GI number: 154244355

Start: 425984

End: 427228

Strand: Reverse

Name: rlmN

Synonym: Xaut_0398

Alternate gene names: 154244355

Gene position: 427228-425984 (Counterclockwise)

Preceding gene: 154244356

Following gene: 154244354

Centisome position: 8.05

GC content: 69.0

Gene sequence:

>1245_bases
ATGATGTCCACACCCGAAACCGCGACCGAAGCCACCGCGCCCGAAGCTGCGCCCGCCCCGTCCCTTGGCGCGGACGCCCC
CGCTCCGCTGCCGTCCCTCATCGGCCTCGACCGGGACAAGCTGGGCGCGGCGCTCGACGCCATCGGCGTGCGCGGCTCCG
ACCGGCGCATGCGGGTGAACCAGCTCTGGCACTGGATCTACCTGCGCGGCGCCACCGATTTCGCGGAGATGACCAACGTC
TCCAAGCACCTGCGCGCCGACCTTGCCGCCGCCTATTCGCTGGCGCGGCCGGAGATCGTGATGGAGCAGGTGTCCCAGGA
CGGCACCCGCAAGTGGCTGCTGCGCTTTCCGGCCGACCATCCCGGCGAGCGGCCCCACGACATCGAGACCGTCTACATCC
CCGAGAGCGACCGGGGCACGCTGTGCGTCTCCAGCCAGGTGGGCTGCACGCTCAACTGCTCGTTCTGCCACACCGGCACC
CAGCGACTGGTCCGCAACCTCACCGCCGCCGAGATCGTGGCCCAGGTGATGGTGGCCCGCGACCGCCTCGGCGACTATCC
CGGCCGCGACCGCGCGACAGGTCCCGGCCTGCCCACCGAGGGCGACCGGCTGGTCACCAACATCGTTTTCATGGGCATGG
GCGAGCCGCTCTATGCCTATGACAGCGTCAAGGAAGCCATCGAGACCCTCTCGGACGGCGATGGCCTCGGCCTTGGCAAG
CGGCGCATCACCGTGTCCACCTCGGGCGTGGTGCCGGAGATCGAGCGGCTGGGCGCCGAAGTCGGCCCCATGCTCGCCAT
CTCGCTCCATGCGGTGCGGGACAAGCTGCGCGACGAACTGGTGCCCATCAACAAGAAGTACCCCATCGCCGAGCTGATGG
AGGCGTGCCGCACCTATCCCGCCGCCTCCAACGCCAAGCGCATCACCTTCGAATACGTCATGCTGAAGGGGGTGAACGAC
AGCCCCGCCGACGCCAGGGCGCTGGTGAAGCTGCTGGAAGGAGTGCCGGCCAAGATCAACCTCATCCCGTTCAATCCCTG
GCCGGGCACGCAGTACGAATGCTCCGACTGGGAGACCATCGAGCGCTTCTCGGACATCGTGTTCCGCGCCGGCTATGCGA
GCCCGGTGCGCACGCCGCGCGGCCGCGACATCCTCGCCGCCTGCGGGCAATTGAAGAGCGAGAGCGAGAAGCTCTCCGCC
CGCGAGCGCCTCGCCCTGCGCGCCATGATGGCGCAGGACGAGTAG

Upstream 100 bases:

>100_bases
CCGGACAGCAGGCACCCCGCGCGAATCCTTGACCAGCCCTGCAATCTCCAGATGGAACTCCAAGTGGAAAGCGCGGGCCG
AAGGCATTATGTGTGACGCC

Downstream 100 bases:

>100_bases
GCCGGCCCGTCCGGACCCTGTCGTGCGACGCTGATAGGCTCGCACGCCGGCGGGGCAAACAGGCTGAGGAGGCCACCATG
CTGGACCATATGGGCGTTTC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MMSTPETATEATAPEAAPAPSLGADAPAPLPSLIGLDRDKLGAALDAIGVRGSDRRMRVNQLWHWIYLRGATDFAEMTNV
SKHLRADLAAAYSLARPEIVMEQVSQDGTRKWLLRFPADHPGERPHDIETVYIPESDRGTLCVSSQVGCTLNCSFCHTGT
QRLVRNLTAAEIVAQVMVARDRLGDYPGRDRATGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVKEAIETLSDGDGLGLGK
RRITVSTSGVVPEIERLGAEVGPMLAISLHAVRDKLRDELVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVND
SPADARALVKLLEGVPAKINLIPFNPWPGTQYECSDWETIERFSDIVFRAGYASPVRTPRGRDILAACGQLKSESEKLSA
RERLALRAMMAQDE

Sequences:

>Translated_414_residues
MMSTPETATEATAPEAAPAPSLGADAPAPLPSLIGLDRDKLGAALDAIGVRGSDRRMRVNQLWHWIYLRGATDFAEMTNV
SKHLRADLAAAYSLARPEIVMEQVSQDGTRKWLLRFPADHPGERPHDIETVYIPESDRGTLCVSSQVGCTLNCSFCHTGT
QRLVRNLTAAEIVAQVMVARDRLGDYPGRDRATGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVKEAIETLSDGDGLGLGK
RRITVSTSGVVPEIERLGAEVGPMLAISLHAVRDKLRDELVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVND
SPADARALVKLLEGVPAKINLIPFNPWPGTQYECSDWETIERFSDIVFRAGYASPVRTPRGRDILAACGQLKSESEKLSA
RERLALRAMMAQDE
>Mature_414_residues
MMSTPETATEATAPEAAPAPSLGADAPAPLPSLIGLDRDKLGAALDAIGVRGSDRRMRVNQLWHWIYLRGATDFAEMTNV
SKHLRADLAAAYSLARPEIVMEQVSQDGTRKWLLRFPADHPGERPHDIETVYIPESDRGTLCVSSQVGCTLNCSFCHTGT
QRLVRNLTAAEIVAQVMVARDRLGDYPGRDRATGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVKEAIETLSDGDGLGLGK
RRITVSTSGVVPEIERLGAEVGPMLAISLHAVRDKLRDELVPINKKYPIAELMEACRTYPAASNAKRITFEYVMLKGVND
SPADARALVKLLEGVPAKINLIPFNPWPGTQYECSDWETIERFSDIVFRAGYASPVRTPRGRDILAACGQLKSESEKLSA
RERLALRAMMAQDE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=362, Percent_Identity=44.7513812154696, Blast_Score=294, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_XANP2 (A7ICB3)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415313.1
- ProteinModelPortal:   A7ICB3
- STRING:   A7ICB3
- GeneID:   5422018
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0398
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TERYLIA
- ProtClustDB:   CLSK979227
- BioCyc:   XAUT78245:XAUT_0398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45275; Mature: 45275

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTPETATEATAPEAAPAPSLGADAPAPLPSLIGLDRDKLGAALDAIGVRGSDRRMRVN
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
QLWHWIYLRGATDFAEMTNVSKHLRADLAAAYSLARPEIVMEQVSQDGTRKWLLRFPADH
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCC
PGERPHDIETVYIPESDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAAEIVAQVMVAR
CCCCCCCCEEEEECCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLGDYPGRDRATGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVKEAIETLSDGDGLGLGK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
RRITVSTSGVVPEIERLGAEVGPMLAISLHAVRDKLRDELVPINKKYPIAELMEACRTYP
EEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
AASNAKRITFEYVMLKGVNDSPADARALVKLLEGVPAKINLIPFNPWPGTQYECSDWETI
CCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
ERFSDIVFRAGYASPVRTPRGRDILAACGQLKSESEKLSARERLALRAMMAQDE
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSTPETATEATAPEAAPAPSLGADAPAPLPSLIGLDRDKLGAALDAIGVRGSDRRMRVN
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
QLWHWIYLRGATDFAEMTNVSKHLRADLAAAYSLARPEIVMEQVSQDGTRKWLLRFPADH
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCC
PGERPHDIETVYIPESDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNLTAAEIVAQVMVAR
CCCCCCCCEEEEECCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLGDYPGRDRATGPGLPTEGDRLVTNIVFMGMGEPLYAYDSVKEAIETLSDGDGLGLGK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
RRITVSTSGVVPEIERLGAEVGPMLAISLHAVRDKLRDELVPINKKYPIAELMEACRTYP
EEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
AASNAKRITFEYVMLKGVNDSPADARALVKLLEGVPAKINLIPFNPWPGTQYECSDWETI
CCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
ERFSDIVFRAGYASPVRTPRGRDILAACGQLKSESEKLSARERLALRAMMAQDE
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA