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The map label for this gene is mltB [H]

Identifier: 154244345

GI number: 154244345

Start: 413517

End: 414848

Strand: Reverse

Name: mltB [H]

Synonym: Xaut_0388

Alternate gene names: 154244345

Gene position: 414848-413517 (Counterclockwise)

Preceding gene: 154244347

Following gene: 154244341

Centisome position: 7.81

GC content: 67.72

Gene sequence:

>1332_bases
ATGGCTCAAACGACCTGCATGCGTGCCCTGAAGGGCACCTTCGTCGCCGGGCTCGCGCTCGCGGCGGCGGGGCTGATCAC
GCCGGCACGGGCGCAGGCCCAGACCACGGGCTCCATCTTCGGCTTCCCGCCGCTGGGCCAGCCCCAGGCGGCACCGGCGA
ACGGCGGCCACCCGCTGATGACGCCCCAGGCGCTGGCGGAGGCCCAGGCCAACTTCCCCGCCTGCATCGCCAATCTGTGG
CCGCTGGCCCAGTCCAAGGGCGTGTCGCGGCGCACCTTCGAGCGCTACACGGCCAACCTCGAACCCCAGATGAAAATCAT
GGAGTTCATGGACAGCCAGCCGGAATTCTCCAAGCCCATCGGCGCCTATGTGAACATGCTCGTCACCGAGACGCGCGTGA
AGAAGGGCCGGGAAATCCTGGAGCGCTACAAGCCCATCTTCGACAGGATGGAAAAGGCCTATGGCGTGGACCGCTATGCG
GTGACGGCCATCTGGGGCATCGAATCCACCTATGGCGACCCCGACGGCATCGGCCGGCGCAACGTGCTGGAATCCACCGC
GACGCTCGCCTGCATCGGCCGGCGGCAGGACTATTTCCGCGACGAGTTCATCGCCACCCTCCAGATCCTGGAGAAGGGCG
ACGTGCCCTATGACCACATGAAGGGCTCGTGGGCCGGCGCGTTCGGCCCCACCCAGTTCATGCCCACCTCGTTCCAGCGC
TTCGCCGTGGACTTCGACGGCGACGGCAAGCGCAACGTGGTGGATTCCATCCCGGACATCATCGCCTCCACCGCCAACAA
CCTGAAGCTGGACGGCTGGGAGTTCGGCAAGGCCTGGGGCTATGAGGTGGTGCTCCCGCAGGGCTTCGACTACCGCAACG
CCAACCGCACCATCAAGCGCACGCTGGGCGAGTGGAACAGCATGGGCCTGCGCCGGCCCAGCGGCGTCGCCTTCCCCCGC
GCCTCCGACACCGCCTACCTGCTGCTGCCCGCCGGGGCCAATGGCCCGGCGTTCCTGATGATGAAGAATTTCGACGCCAT
CCTGAAGTACAACCCCGCCGACGCCTACGCCCTCGCCATCGGGCACCTGGCGGACCGGCTGCGGGGCGGCGGCACCTTCG
TCCAGTCCTGGCCGAAGGAAGAGCGCGGCCTCTCCAAGGCCGAGCGGCAGGAGATCCAGTCCCGCCTCGCGCAGATGGGC
TACGACATCGGCAACGTGGACGGCATCCTCGGCCAGAAGACCCGCGTCGCCATCCAGGACTACCAGGTGCGCTCGGGCGC
TCTGCCCGACGGCTATCCCGACCTGCAACTGCTCCAGCAGATGCGGCGCTGA

Upstream 100 bases:

>100_bases
ATCCATTAACCGCGACGCAACCATATTGCCGCCCAGTAGAGGCCTATCGGTGAGGCGAGGCGGCGGCAGAAGGACGCGCG
CCGGGGAGGACAGCTTGGCA

Downstream 100 bases:

>100_bases
CCGGCTTCCGCTCCCCTAAAGAAGAACCCGCCGGCGCAACCCGGCGGGTTTTTCTTTGGGTCACGTGCAGACCGCCCTCA
CTCCAGCCCGATGCGGTAGT

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAQTTCMRALKGTFVAGLALAAAGLITPARAQAQTTGSIFGFPPLGQPQAAPANGGHPLMTPQALAEAQANFPACIANLW
PLAQSKGVSRRTFERYTANLEPQMKIMEFMDSQPEFSKPIGAYVNMLVTETRVKKGREILERYKPIFDRMEKAYGVDRYA
VTAIWGIESTYGDPDGIGRRNVLESTATLACIGRRQDYFRDEFIATLQILEKGDVPYDHMKGSWAGAFGPTQFMPTSFQR
FAVDFDGDGKRNVVDSIPDIIASTANNLKLDGWEFGKAWGYEVVLPQGFDYRNANRTIKRTLGEWNSMGLRRPSGVAFPR
ASDTAYLLLPAGANGPAFLMMKNFDAILKYNPADAYALAIGHLADRLRGGGTFVQSWPKEERGLSKAERQEIQSRLAQMG
YDIGNVDGILGQKTRVAIQDYQVRSGALPDGYPDLQLLQQMRR

Sequences:

>Translated_443_residues
MAQTTCMRALKGTFVAGLALAAAGLITPARAQAQTTGSIFGFPPLGQPQAAPANGGHPLMTPQALAEAQANFPACIANLW
PLAQSKGVSRRTFERYTANLEPQMKIMEFMDSQPEFSKPIGAYVNMLVTETRVKKGREILERYKPIFDRMEKAYGVDRYA
VTAIWGIESTYGDPDGIGRRNVLESTATLACIGRRQDYFRDEFIATLQILEKGDVPYDHMKGSWAGAFGPTQFMPTSFQR
FAVDFDGDGKRNVVDSIPDIIASTANNLKLDGWEFGKAWGYEVVLPQGFDYRNANRTIKRTLGEWNSMGLRRPSGVAFPR
ASDTAYLLLPAGANGPAFLMMKNFDAILKYNPADAYALAIGHLADRLRGGGTFVQSWPKEERGLSKAERQEIQSRLAQMG
YDIGNVDGILGQKTRVAIQDYQVRSGALPDGYPDLQLLQQMRR
>Mature_442_residues
AQTTCMRALKGTFVAGLALAAAGLITPARAQAQTTGSIFGFPPLGQPQAAPANGGHPLMTPQALAEAQANFPACIANLWP
LAQSKGVSRRTFERYTANLEPQMKIMEFMDSQPEFSKPIGAYVNMLVTETRVKKGREILERYKPIFDRMEKAYGVDRYAV
TAIWGIESTYGDPDGIGRRNVLESTATLACIGRRQDYFRDEFIATLQILEKGDVPYDHMKGSWAGAFGPTQFMPTSFQRF
AVDFDGDGKRNVVDSIPDIIASTANNLKLDGWEFGKAWGYEVVLPQGFDYRNANRTIKRTLGEWNSMGLRRPSGVAFPRA
SDTAYLLLPAGANGPAFLMMKNFDAILKYNPADAYALAIGHLADRLRGGGTFVQSWPKEERGLSKAERQEIQSRLAQMGY
DIGNVDGILGQKTRVAIQDYQVRSGALPDGYPDLQLLQQMRR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=312, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 48888; Mature: 48757

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTTCMRALKGTFVAGLALAAAGLITPARAQAQTTGSIFGFPPLGQPQAAPANGGHPLM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCCCCCCCCCCCCC
TPQALAEAQANFPACIANLWPLAQSKGVSRRTFERYTANLEPQMKIMEFMDSQPEFSKPI
CHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCH
GAYVNMLVTETRVKKGREILERYKPIFDRMEKAYGVDRYAVTAIWGIESTYGDPDGIGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCH
NVLESTATLACIGRRQDYFRDEFIATLQILEKGDVPYDHMKGSWAGAFGPTQFMPTSFQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
FAVDFDGDGKRNVVDSIPDIIASTANNLKLDGWEFGKAWGYEVVLPQGFDYRNANRTIKR
EEEECCCCCCCHHHHHHHHHHHHCCCCCEECCEECCCCCCEEEEECCCCCCCCCCHHHHH
TLGEWNSMGLRRPSGVAFPRASDTAYLLLPAGANGPAFLMMKNFDAILKYNPADAYALAI
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEEEEECCCHHHHHHH
GHLADRLRGGGTFVQSWPKEERGLSKAERQEIQSRLAQMGYDIGNVDGILGQKTRVAIQD
HHHHHHHCCCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEH
YQVRSGALPDGYPDLQLLQQMRR
EEECCCCCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
AQTTCMRALKGTFVAGLALAAAGLITPARAQAQTTGSIFGFPPLGQPQAAPANGGHPLM
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEECCCCCCCCCCCCCCCCCCCC
TPQALAEAQANFPACIANLWPLAQSKGVSRRTFERYTANLEPQMKIMEFMDSQPEFSKPI
CHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCH
GAYVNMLVTETRVKKGREILERYKPIFDRMEKAYGVDRYAVTAIWGIESTYGDPDGIGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCH
NVLESTATLACIGRRQDYFRDEFIATLQILEKGDVPYDHMKGSWAGAFGPTQFMPTSFQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
FAVDFDGDGKRNVVDSIPDIIASTANNLKLDGWEFGKAWGYEVVLPQGFDYRNANRTIKR
EEEECCCCCCCHHHHHHHHHHHHCCCCCEECCEECCCCCCEEEEECCCCCCCCCCHHHHH
TLGEWNSMGLRRPSGVAFPRASDTAYLLLPAGANGPAFLMMKNFDAILKYNPADAYALAI
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCEEEEECCCHHHHHHH
GHLADRLRGGGTFVQSWPKEERGLSKAERQEIQSRLAQMGYDIGNVDGILGQKTRVAIQD
HHHHHHHCCCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEH
YQVRSGALPDGYPDLQLLQQMRR
EEECCCCCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]