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The map label for this gene is hpaH [H]

Identifier: 154244334

GI number: 154244334

Start: 391526

End: 393010

Strand: Reverse

Name: hpaH [H]

Synonym: Xaut_0377

Alternate gene names: 154244334

Gene position: 393010-391526 (Counterclockwise)

Preceding gene: 154244335

Following gene: 154244333

Centisome position: 7.4

GC content: 66.2

Gene sequence:

>1485_bases
ATGATCAAGACCGGCAGCGCCCACATCGCCAGCCTCAGGGACGGCCGCGAGATCTTCCTCGACGGCCAGAAGATTTCCGA
CGCCACCACCCACCCCGCCTTCCGCCGCGCGGTGGCCTCGGTGGGGCGCATGTTCGACTTCCACAGCGCGCCGGACAACC
GCGCGCTGATGACCTTCGAGACCGACACCGGCACGCTCGCCAACCGCATCTGGCAGTTGCCCACCAGCTATGACGAGCTG
AAGACGCGCCGCCGGGGCCTTGAGGCTTGGACCGAGCTGCACGCCGGCTTCCTCGGCCGCGCGCCGGACCATGTGGCGTC
CTGCATCTCCGGCATGTACATGGGCCTCGACCAGTTCGAGCTGTACGATCCGGCCCGTGCCCGGGCGCTTGGCGATTACT
ATCGCCATGCGCGCGACAACGATCTGTATCTCACCTACGTCATCATCAACCCGCAGGCCGACCGCTCCAAGAACGCGGCC
GAGCAGGCCGACCCGTTCCTCTCCGCCGCCATCGTGGACGAGGACACCTCCGGCATCACCGTGCGCGGGGCGAAGATGCT
CGCCACCGGCGGCATCATGGCCAATGAGGTGTTCGTCACCTGCATCCAGCCGCTGCAGAAGGGCGAGGAGGCCTACGCCC
TCTCCTTCGCCATTCCCATGAACACGAAGGGCCTGAAGATCCTGTCGCGCAAGTCCTATGAGGCAGGCGCACCGTCGGTG
TTCGACAATCCCCTGTCGAGCCGCTTCGACGAGAATGACGCGGTGCTGTATTTCGACGATGTGAAGGTGCCGTGGGATCG
GGTCTTCATCGCCGGCGACATCGCCATGACCGGCCGGCAGTTCCATGCCACCCCGGCCCATGTCTACCAGAACTACCAGG
CCCAGATCCGCCTGTCGGTAAAGCTGAAATTCCTGCTCGCCATCGCCCGGCGCACGGCCGAGGTGAACGGCACCACCGGC
TTCCCGCAGGTGCGCGAGACCTTGGGCCAGCTCGCCGCCGAGGCCGCCATGGTGGATGCCTTCGTCGCCGCCATGGAGGC
CAAGGGCAGCTTCTACGGCCCCTATTTCGTGCCCGACCGCCACACCCTCTACGCCGCGCAGACCCTGACCCAGCAGCTCT
ACGGCAAGTTCATCACCAGCCTGCGGGAATTGGCCGGCGGCGGCATGATCATGCTGCCCTCCTCGGTGCATGACTATCAG
AACCCGGAGCTGGCCGCCCTCATCGCCAAGACCCAGCAGTCGCCGGCTGCCAGCGCCGAAGAAAAGGTGAAGTTCTACAA
GCTCGCCTGGGACGCGGTGGGCTCGGAATTCGCCTCCCGCCACGCCCAGTACGAGATGTTCTATGCGGGGGCCGGCTTCG
TGGTGAAGAACCACTCCTTCCGCACCTATGACTGGGCCGGCGCCGAGCGCCTGCTCGACATGATGCTTTCCTCCTATTCC
CTCGCCGACGAGGTGGCCCCGACCGCCGCCCGCGCCGCCGAGTGA

Upstream 100 bases:

>100_bases
ATCGCTGCGCGCCGGGGGCTGATCCCCTCGTCTTCCATGCCGGACGCTACCGCAGCCTTGCGGCCGCCGCGTCCTGACCC
CCATTTCCGGAGCACCCGCC

Downstream 100 bases:

>100_bases
TCCCCTTTCACCGCAGGACTGCCCCGATGCCCATCACCAAGATCCACGACGAGTTCCACACCCTCGACCTTGCCACCGGC
TGGGAAGTGCCGCCGGGCTA

Product: 4-hydroxyphenylacetate 3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MIKTGSAHIASLRDGREIFLDGQKISDATTHPAFRRAVASVGRMFDFHSAPDNRALMTFETDTGTLANRIWQLPTSYDEL
KTRRRGLEAWTELHAGFLGRAPDHVASCISGMYMGLDQFELYDPARARALGDYYRHARDNDLYLTYVIINPQADRSKNAA
EQADPFLSAAIVDEDTSGITVRGAKMLATGGIMANEVFVTCIQPLQKGEEAYALSFAIPMNTKGLKILSRKSYEAGAPSV
FDNPLSSRFDENDAVLYFDDVKVPWDRVFIAGDIAMTGRQFHATPAHVYQNYQAQIRLSVKLKFLLAIARRTAEVNGTTG
FPQVRETLGQLAAEAAMVDAFVAAMEAKGSFYGPYFVPDRHTLYAAQTLTQQLYGKFITSLRELAGGGMIMLPSSVHDYQ
NPELAALIAKTQQSPAASAEEKVKFYKLAWDAVGSEFASRHAQYEMFYAGAGFVVKNHSFRTYDWAGAERLLDMMLSSYS
LADEVAPTAARAAE

Sequences:

>Translated_494_residues
MIKTGSAHIASLRDGREIFLDGQKISDATTHPAFRRAVASVGRMFDFHSAPDNRALMTFETDTGTLANRIWQLPTSYDEL
KTRRRGLEAWTELHAGFLGRAPDHVASCISGMYMGLDQFELYDPARARALGDYYRHARDNDLYLTYVIINPQADRSKNAA
EQADPFLSAAIVDEDTSGITVRGAKMLATGGIMANEVFVTCIQPLQKGEEAYALSFAIPMNTKGLKILSRKSYEAGAPSV
FDNPLSSRFDENDAVLYFDDVKVPWDRVFIAGDIAMTGRQFHATPAHVYQNYQAQIRLSVKLKFLLAIARRTAEVNGTTG
FPQVRETLGQLAAEAAMVDAFVAAMEAKGSFYGPYFVPDRHTLYAAQTLTQQLYGKFITSLRELAGGGMIMLPSSVHDYQ
NPELAALIAKTQQSPAASAEEKVKFYKLAWDAVGSEFASRHAQYEMFYAGAGFVVKNHSFRTYDWAGAERLLDMMLSSYS
LADEVAPTAARAAE
>Mature_494_residues
MIKTGSAHIASLRDGREIFLDGQKISDATTHPAFRRAVASVGRMFDFHSAPDNRALMTFETDTGTLANRIWQLPTSYDEL
KTRRRGLEAWTELHAGFLGRAPDHVASCISGMYMGLDQFELYDPARARALGDYYRHARDNDLYLTYVIINPQADRSKNAA
EQADPFLSAAIVDEDTSGITVRGAKMLATGGIMANEVFVTCIQPLQKGEEAYALSFAIPMNTKGLKILSRKSYEAGAPSV
FDNPLSSRFDENDAVLYFDDVKVPWDRVFIAGDIAMTGRQFHATPAHVYQNYQAQIRLSVKLKFLLAIARRTAEVNGTTG
FPQVRETLGQLAAEAAMVDAFVAAMEAKGSFYGPYFVPDRHTLYAAQTLTQQLYGKFITSLRELAGGGMIMLPSSVHDYQ
NPELAALIAKTQQSPAASAEEKVKFYKLAWDAVGSEFASRHAQYEMFYAGAGFVVKNHSFRTYDWAGAERLLDMMLSSYS
LADEVAPTAARAAE

Specific function: Utilizes FADH(2) supplied by hpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 54574; Mature: 54574

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTGSAHIASLRDGREIFLDGQKISDATTHPAFRRAVASVGRMFDFHSAPDNRALMTFE
CCCCCCCHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TDTGTLANRIWQLPTSYDELKTRRRGLEAWTELHAGFLGRAPDHVASCISGMYMGLDQFE
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHC
LYDPARARALGDYYRHARDNDLYLTYVIINPQADRSKNAAEQADPFLSAAIVDEDTSGIT
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHEEEEECCCCCCEE
VRGAKMLATGGIMANEVFVTCIQPLQKGEEAYALSFAIPMNTKGLKILSRKSYEAGAPSV
EECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCH
FDNPLSSRFDENDAVLYFDDVKVPWDRVFIAGDIAMTGRQFHATPAHVYQNYQAQIRLSV
HCCCHHHCCCCCCCEEEEECCCCCHHEEEEEECEEECCCCCCCCHHHHHCCCEEEEEEEE
KLKFLLAIARRTAEVNGTTGFPQVRETLGQLAAEAAMVDAFVAAMEAKGSFYGPYFVPDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
HTLYAAQTLTQQLYGKFITSLRELAGGGMIMLPSSVHDYQNPELAALIAKTQQSPAASAE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHH
EKVKFYKLAWDAVGSEFASRHAQYEMFYAGAGFVVKNHSFRTYDWAGAERLLDMMLSSYS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCEEECCCCHHHHHHHHHHHHH
LADEVAPTAARAAE
HHHHHCCCHHHCCC
>Mature Secondary Structure
MIKTGSAHIASLRDGREIFLDGQKISDATTHPAFRRAVASVGRMFDFHSAPDNRALMTFE
CCCCCCCHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TDTGTLANRIWQLPTSYDELKTRRRGLEAWTELHAGFLGRAPDHVASCISGMYMGLDQFE
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHC
LYDPARARALGDYYRHARDNDLYLTYVIINPQADRSKNAAEQADPFLSAAIVDEDTSGIT
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHEEEEECCCCCCEE
VRGAKMLATGGIMANEVFVTCIQPLQKGEEAYALSFAIPMNTKGLKILSRKSYEAGAPSV
EECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCH
FDNPLSSRFDENDAVLYFDDVKVPWDRVFIAGDIAMTGRQFHATPAHVYQNYQAQIRLSV
HCCCHHHCCCCCCCEEEEECCCCCHHEEEEEECEEECCCCCCCCHHHHHCCCEEEEEEEE
KLKFLLAIARRTAEVNGTTGFPQVRETLGQLAAEAAMVDAFVAAMEAKGSFYGPYFVPDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
HTLYAAQTLTQQLYGKFITSLRELAGGGMIMLPSSVHDYQNPELAALIAKTQQSPAASAE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHH
EKVKFYKLAWDAVGSEFASRHAQYEMFYAGAGFVVKNHSFRTYDWAGAERLLDMMLSSYS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCEEECCCCHHHHHHHHHHHHH
LADEVAPTAARAAE
HHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA