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The map label for this gene is gatA [H]

Identifier: 154244332

GI number: 154244332

Start: 389740

End: 391083

Strand: Reverse

Name: gatA [H]

Synonym: Xaut_0375

Alternate gene names: 154244332

Gene position: 391083-389740 (Counterclockwise)

Preceding gene: 154244333

Following gene: 154244329

Centisome position: 7.37

GC content: 74.26

Gene sequence:

>1344_bases
ATGCCCCAGCCGACCCTTGCCGCCCTCGCCGAAGACCTCGCCTCCGGGCGCACCACGGCGCGGGCGCTGGCGGAAGCCTG
CCTCGATCGCATTGCCGACCCCGCCGGCGAAGGCGCCCGCGCCTTCGTCAGCGTGAACCGGGAGGGGGCGCTGAAGGCGG
CGCAGGCGCAGGACGAACTGCGGCAGGCGGGGGCGGCGCCCTCGCCCTTCGCCGGCATCCCCATTTCGGTGAAGGACCTG
TTCGACGTGGCCGGCGAGGTCACCGCCGCCGGCTCCAAGGTGCTGAAGGACAATGCCCCGGCGACGGCGGATGCCCCGGC
GGTGGCGCGCCTGCGCCGGGCGGGGTTCGTGCTCCTCGGCCGCAACACCATGACCGAGTTCGCCTATTCCGGCCTCGGCA
TGAACCCGCATTATGGCCACCCGCGCGCCCCGTTCGAGCGCGCGGAAGGCGGCCGCGTCTCCGGCGGCTCCAGCTCCGGC
GGGGCGGTGGCGGTGGCGGACGGCATGGCCCACGCGGCGCTCGGCACCGACACCGGCGGCTCCTGCCGCATCCCGGCCGC
CTTCTGCGGCATCACCGGCTACAAGCCCACCGCCAGCCGGGTGCCGCGCGAGGGCGCCTTCCCTCTCTCCACGACGCTGG
ATTCCATCGGCCCCATCGCCCGCTCGGTGGCCTGCTGCGTGGCGCTGGACGCCATCCTCTCAGGCAGCGAGCCGGCGCCG
CTGGCGCCACGCCCGGTCAAGGGGCTGCGCCTGCTTGTACCCACCACCGTGGCGCTCGACGGGCTCGACCCCGAGGTGGC
CAAGGCCTTCGAGGCGGCGGTGGAGGCGCTGGCGCACGCCGGCGCGCATATCGTCTCCGCGCCCTTCCCGGAGTTCGGCG
AAGTGGCGACCATCAATTCCAAGGGTGGCTTCTCGGCCGCCGAGAGCTATGCAGTGCACCGCCGCCTGCTGGCGGAGAAG
GCCGACGCCTACGACCCGCGTGTCGCCGGCCGCATCAAGCGCGGGGCGGAGCAGTCCGCCGCCGATTATGTGGAACTGGT
GGCCGCCCGCCGGGCGCTGGTTGCGCGGGCCGCGGCGCGGCTTGCCGGCTTCGACGCACTGGTGATGCCCACGGTGGCCA
TCCTGCCGCCGAGGATCGCGGATCTCGCCACCGACGACGACGCCTATGGCCGCGCCAACCTGCTGGCCCTGCGCAACCCC
ACCCTCATCAACATGATCGACGGCTGCGCCATCTCGCTGCCGTTCCCCGGCGCGCCCGTGGGGCTGATGCTGGCCGGCGC
GCCGGGCACCGATCAGTCCCTCTTCGCCGTCGCGGCGGGGGTGGAGGCGGCGCTCTCCGCCTGA

Upstream 100 bases:

>100_bases
ACGGGCCGTTCAAATCGGATAAGGGCTGCATGCTGCTCGAAATCCACTATTTCGACCCGGCCTGATCCCGCCCGCCCCTT
CTGCTGACCCGGACTTCGCC

Downstream 100 bases:

>100_bases
TCGGCCGAGCCCGCCTTCGGCAGCAGCACCCGCAGGCGCGCGCCACCGCCCGGACGATCGTCGATGACGACCTCGCCGCC
GTGCCCCTCCACGATCTGCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPQPTLAALAEDLASGRTTARALAEACLDRIADPAGEGARAFVSVNREGALKAAQAQDELRQAGAAPSPFAGIPISVKDL
FDVAGEVTAAGSKVLKDNAPATADAPAVARLRRAGFVLLGRNTMTEFAYSGLGMNPHYGHPRAPFERAEGGRVSGGSSSG
GAVAVADGMAHAALGTDTGGSCRIPAAFCGITGYKPTASRVPREGAFPLSTTLDSIGPIARSVACCVALDAILSGSEPAP
LAPRPVKGLRLLVPTTVALDGLDPEVAKAFEAAVEALAHAGAHIVSAPFPEFGEVATINSKGGFSAAESYAVHRRLLAEK
ADAYDPRVAGRIKRGAEQSAADYVELVAARRALVARAAARLAGFDALVMPTVAILPPRIADLATDDDAYGRANLLALRNP
TLINMIDGCAISLPFPGAPVGLMLAGAPGTDQSLFAVAAGVEAALSA

Sequences:

>Translated_447_residues
MPQPTLAALAEDLASGRTTARALAEACLDRIADPAGEGARAFVSVNREGALKAAQAQDELRQAGAAPSPFAGIPISVKDL
FDVAGEVTAAGSKVLKDNAPATADAPAVARLRRAGFVLLGRNTMTEFAYSGLGMNPHYGHPRAPFERAEGGRVSGGSSSG
GAVAVADGMAHAALGTDTGGSCRIPAAFCGITGYKPTASRVPREGAFPLSTTLDSIGPIARSVACCVALDAILSGSEPAP
LAPRPVKGLRLLVPTTVALDGLDPEVAKAFEAAVEALAHAGAHIVSAPFPEFGEVATINSKGGFSAAESYAVHRRLLAEK
ADAYDPRVAGRIKRGAEQSAADYVELVAARRALVARAAARLAGFDALVMPTVAILPPRIADLATDDDAYGRANLLALRNP
TLINMIDGCAISLPFPGAPVGLMLAGAPGTDQSLFAVAAGVEAALSA
>Mature_446_residues
PQPTLAALAEDLASGRTTARALAEACLDRIADPAGEGARAFVSVNREGALKAAQAQDELRQAGAAPSPFAGIPISVKDLF
DVAGEVTAAGSKVLKDNAPATADAPAVARLRRAGFVLLGRNTMTEFAYSGLGMNPHYGHPRAPFERAEGGRVSGGSSSGG
AVAVADGMAHAALGTDTGGSCRIPAAFCGITGYKPTASRVPREGAFPLSTTLDSIGPIARSVACCVALDAILSGSEPAPL
APRPVKGLRLLVPTTVALDGLDPEVAKAFEAAVEALAHAGAHIVSAPFPEFGEVATINSKGGFSAAESYAVHRRLLAEKA
DAYDPRVAGRIKRGAEQSAADYVELVAARRALVARAAARLAGFDALVMPTVAILPPRIADLATDDDAYGRANLLALRNPT
LINMIDGCAISLPFPGAPVGLMLAGAPGTDQSLFAVAAGVEAALSA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=510, Percent_Identity=24.5098039215686, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI195972892, Length=224, Percent_Identity=31.25, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=26.2068965517241, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=390, Percent_Identity=25.6410256410256, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=481, Percent_Identity=23.2848232848233, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=202, Percent_Identity=31.6831683168317, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=312, Percent_Identity=30.4487179487179, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=312, Percent_Identity=30.4487179487179, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=187, Percent_Identity=29.9465240641711, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=391, Percent_Identity=28.6445012787724, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=148, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=27.6209677419355, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648435, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648437, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI45550774, Length=285, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648441, Length=236, Percent_Identity=29.2372881355932, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648439, Length=236, Percent_Identity=29.2372881355932, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=23.4468937875752, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644968, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652985, Length=247, Percent_Identity=28.7449392712551, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922090, Length=247, Percent_Identity=28.7449392712551, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652981, Length=247, Percent_Identity=28.7449392712551, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652983, Length=247, Percent_Identity=28.7449392712551, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21356731, Length=158, Percent_Identity=34.1772151898734, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 45310; Mature: 45179

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPTLAALAEDLASGRTTARALAEACLDRIADPAGEGARAFVSVNREGALKAAQAQDEL
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
RQAGAAPSPFAGIPISVKDLFDVAGEVTAAGSKVLKDNAPATADAPAVARLRRAGFVLLG
HHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCEEEEC
RNTMTEFAYSGLGMNPHYGHPRAPFERAEGGRVSGGSSSGGAVAVADGMAHAALGTDTGG
CCHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
SCRIPAAFCGITGYKPTASRVPREGAFPLSTTLDSIGPIARSVACCVALDAILSGSEPAP
CEECCHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAPRPVKGLRLLVPTTVALDGLDPEVAKAFEAAVEALAHAGAHIVSAPFPEFGEVATINS
CCCCCCCCEEEEECHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECC
KGGFSAAESYAVHRRLLAEKADAYDPRVAGRIKRGAEQSAADYVELVAARRALVARAAAR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGFDALVMPTVAILPPRIADLATDDDAYGRANLLALRNPTLINMIDGCAISLPFPGAPV
HHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCEEECCCCCCCC
GLMLAGAPGTDQSLFAVAAGVEAALSA
EEEEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQPTLAALAEDLASGRTTARALAEACLDRIADPAGEGARAFVSVNREGALKAAQAQDEL
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
RQAGAAPSPFAGIPISVKDLFDVAGEVTAAGSKVLKDNAPATADAPAVARLRRAGFVLLG
HHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHCCEEEEC
RNTMTEFAYSGLGMNPHYGHPRAPFERAEGGRVSGGSSSGGAVAVADGMAHAALGTDTGG
CCHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
SCRIPAAFCGITGYKPTASRVPREGAFPLSTTLDSIGPIARSVACCVALDAILSGSEPAP
CEECCHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAPRPVKGLRLLVPTTVALDGLDPEVAKAFEAAVEALAHAGAHIVSAPFPEFGEVATINS
CCCCCCCCEEEEECHHEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECC
KGGFSAAESYAVHRRLLAEKADAYDPRVAGRIKRGAEQSAADYVELVAARRALVARAAAR
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGFDALVMPTVAILPPRIADLATDDDAYGRANLLALRNPTLINMIDGCAISLPFPGAPV
HHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCEEECCCCCCCC
GLMLAGAPGTDQSLFAVAAGVEAALSA
EEEEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA