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The map label for this gene is mraW [H]

Identifier: 153952604

GI number: 153952604

Start: 1159562

End: 1160494

Strand: Direct

Name: mraW [H]

Synonym: JJD26997_1313

Alternate gene names: 153952604

Gene position: 1159562-1160494 (Clockwise)

Preceding gene: 153952403

Following gene: 153951509

Centisome position: 62.85

GC content: 28.08

Gene sequence:

>933_bases
TTGGAAATTCCTCATATCCCAGTTTTGCTTAATGAAGTTCAAGAGATTTTTAAAAATCTTAAAACAGGTTATTTTTTAGA
CTGCACTTTAGGATTTGGCGGACATAGTGAAACTTTGCTTAAAAATCATCCTGGTCTTAAATTCATTGCTTGTGATCAAG
ATCAACAAGCTTTAGAATTTTCTAAAAAACGTCTTAAAGACTTTCATAATAGGATAACTTTTATACAAAGTAATTTTAGT
GAAGTTTTAGAAAAAATTTCACATAAAGAAGAATTAAGAGGTATTTTAGCAGACATTGGAGTTTCTTCATTTCAACTTGA
TAATAACGAAAGAGGTTTTAGTGTAAATTCTGACTTTTTGGATATGAGAATGAATCAAAATTCCAAAATATCGGCTTATG
AGGTTGTTAATGCTTATACAAAAGAGCAATTAACTTCCATTTTTAAAGATTATGGAGAATTGCACGATGCACATTTTATT
GCTGAAAAAATTTGCCTAGAAAGAAGTAAAAATCTAATCAAAAGCGCCAAGGAACTTTATCAAATCATAGGAAAAGGCAA
ACAAAATCATAGAAAAATTTCCAAAGCTACTTTAGCATTTCAAGCCATTAGAATAGAAGTAAATCAAGAACTTAAAGTAC
TTAAAGATTTTCTTGGATATTTAGAAAATTTAAAACCAAAAAATTGCATTTTAGCAATCATTAGTTTTCATTCTTTGGAA
GATAGAATAGTAAAAAATTTTTTCAAAAAATGGTCAAAAAACTGCATTTGTGATGAAAAAATAATGCGTTGCAAATGTGG
AAATAACCATAGCCTAGGGCAAATAATAACCAAAAAATCTATAAGCGCAAGCAAGGAAGAACTTTTAAAAAATTCTCGCT
CTAGTTGTGCTAAAATGCGTGCTTTTTATTTTAATAATTTGGACAATAAATAA

Upstream 100 bases:

>100_bases
ATAAAATTTTTACCAAAAAATATTTGTTCTTTAAATTTTATAATTTTTACAATGAAATTAAGTTATAATTATAGTTTATA
AAAATTTATTAGGATTGATT

Downstream 100 bases:

>100_bases
TGCTAGAAGATGATTTTATAAAAGAACGCCAAAACATACGCCAAAAAATGCTGAAATTTTCTCGCGCTATAAATCAAGGC
AAACCGCTTGATGATGATTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSETLLKNHPGLKFIACDQDQQALEFSKKRLKDFHNRITFIQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEVVNAYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNLIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGYLENLKPKNCILAIISFHSLE
DRIVKNFFKKWSKNCICDEKIMRCKCGNNHSLGQIITKKSISASKEELLKNSRSSCAKMRAFYFNNLDNK

Sequences:

>Translated_310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSETLLKNHPGLKFIACDQDQQALEFSKKRLKDFHNRITFIQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEVVNAYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNLIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGYLENLKPKNCILAIISFHSLE
DRIVKNFFKKWSKNCICDEKIMRCKCGNNHSLGQIITKKSISASKEELLKNSRSSCAKMRAFYFNNLDNK
>Mature_310_residues
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSETLLKNHPGLKFIACDQDQQALEFSKKRLKDFHNRITFIQSNFS
EVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFLDMRMNQNSKISAYEVVNAYTKEQLTSIFKDYGELHDAHFI
AEKICLERSKNLIKSAKELYQIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGYLENLKPKNCILAIISFHSLE
DRIVKNFFKKWSKNCICDEKIMRCKCGNNHSLGQIITKKSISASKEELLKNSRSSCAKMRAFYFNNLDNK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=32.1321321321321, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=31.6582914572864, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=32.5806451612903, Blast_Score=139, Evalue=3e-34,
Organism=Drosophila melanogaster, GI62472493, Length=341, Percent_Identity=30.4985337243402, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571637, Length=341, Percent_Identity=30.4985337243402, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35740; Mature: 35740

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSETLLKNHPGLKFIACDQDQQALEF
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCCCEEEEECCCHHHHHH
SKKRLKDFHNRITFIQSNFSEVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
DMRMNQNSKISAYEVVNAYTKEQLTSIFKDYGELHDAHFIAEKICLERSKNLIKSAKELY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGYLENLKPKNCILAIISFHSLE
HHHHCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DRIVKNFFKKWSKNCICDEKIMRCKCGNNHSLGQIITKKSISASKEELLKNSRSSCAKMR
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AFYFNNLDNK
HHHHHCCCCC
>Mature Secondary Structure
MEIPHIPVLLNEVQEIFKNLKTGYFLDCTLGFGGHSETLLKNHPGLKFIACDQDQQALEF
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCCCEEEEECCCHHHHHH
SKKRLKDFHNRITFIQSNFSEVLEKISHKEELRGILADIGVSSFQLDNNERGFSVNSDFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
DMRMNQNSKISAYEVVNAYTKEQLTSIFKDYGELHDAHFIAEKICLERSKNLIKSAKELY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIIGKGKQNHRKISKATLAFQAIRIEVNQELKVLKDFLGYLENLKPKNCILAIISFHSLE
HHHHCCCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
DRIVKNFFKKWSKNCICDEKIMRCKCGNNHSLGQIITKKSISASKEELLKNSRSSCAKMR
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AFYFNNLDNK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]