Click here to switch to the map view.

The map label for this gene is livK [H]

Identifier: 153949569

GI number: 153949569

Start: 273152

End: 274267

Strand: Direct

Name: livK [H]

Synonym: YpsIP31758_0244

Alternate gene names: 153949569

Gene position: 273152-274267 (Clockwise)

Preceding gene: 153947554

Following gene: 153947361

Centisome position: 5.78

GC content: 49.46

Gene sequence:

>1116_bases
ATGAAATTAACAAAAGGTAAAGCGTTGCTGGCTGGGTGTATGGCAATGGCGATGAGTCATTCTGTGCTCGCGCAAGACAT
TAAGGTCGCTATTGTGGGGGCAATGTCCGGCCCGGTGGCCCAATACGGTGACATGCAATTTACCGGTGCGCGCCAGGCTA
TCGCCGATATCAACGCTAAAGGTGGTATTAAAGGCGATAAGCTGGTTGGTGTGGAATACGATGATGCCTGCGATCCGAAA
CAAGCCGTGGCTGTCGCGAACAAAGTAATAAACGATGGTATCCGTTATGTTATCGGCCATCTGTGCTCTTCTTCAACTCA
GCCAGCTTCAGATATTTATGAAGATGAAGGGGTGATAATGATCACCCCTGCTGCTACAAACGCGGATCTGACCACCCGTG
GTTACAAAATGATTATGCGCACCACCGGCCTGGATTCAGACCAGGGCCCTACTGCTGCCAAATATATTGTTGAGACGATT
AAACCTAAACGTATCGCTGTGGTACATGATAAACAGCAATACGGTGAAGGTCTGGCACGTTCCGTGCGCGACAGTCTGAA
AGAACAAGGTGCAGAGGTTGTGCTGTTTGAAGGTGTGACCGCAGGGGATAAAGATTTCTCGACTCTGGTTGCTCGCCTGA
AGAAAGAGAACGTCGATTTTGTTTATTTCGGCGGTTACTACCCAGAAATGGGTCAAATTCTGCGTCAGGCTAAGCAAGCA
GGCCTAACCGCCCGCTTTATGGGGCCAGAAGGTGTGGGTAACTCCTCACTCTCCAATATCGCGGGTGAGGCTTCTGAAGG
CATGCTGGTGACATTACCTAAGCGTTATGATCAGGTTCCGGCTAACCAACCTATTGTTGATGCGCTAAAAGCGAAAAAAC
TGGATCCAACAGGCCCATTTGTGTGGACCACTTATGCTGCGCTGCAATCACTGACGACGGCGATGGAACGTACTGGTAGC
AAAGAACCGGCTGATTTGGCTAACGATCTGAAAACCGGCAAACCGGTTGAGACCGTGATGGGGCCATTGAGCTGGGACGA
TAAAGGCGATTTGAAAGGGTTTGAGTTTGGTATTTTTGAATGGCATGCGGATGGTTCTTCTACCGCAGTCAAATAA

Upstream 100 bases:

>100_bases
GTTAAATCATTATTACTTATGAACAAAATGTTACATTAATGCTAAAACACTCAGACAAACACAACAAGCATGTTCACAAT
GACAGATGGGGAATTCGCGG

Downstream 100 bases:

>100_bases
CAAGAATAGTAGAACTATTGACAAGAATAGTAGAACTATCTCGTTTTAGGATTGCTCTCGCAGTAATCCGGCCCCTTCTA
TCAACTGCATCCCCTCGTAT

Product: high-affinity branched-chain amino acid ABC transporter periplasmic protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLTKGKALLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Sequences:

>Translated_371_residues
MKLTKGKALLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK
>Mature_371_residues
MKLTKGKALLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAKGGIKGDKLVGVEYDDACDPK
QAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETI
KPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAMERTGS
KEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=75.6164383561644, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=75.2043596730245, Blast_Score=586, Evalue=1e-169,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39644; Mature: 39644

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKGKALLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAK
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGSSTAVK
EECCCCCCCCC
>Mature Secondary Structure
MKLTKGKALLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINAK
CCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHHHCCCC
GGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIM
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEE
ITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYGEGLAR
EECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
SVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQAKQA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHC
GLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPF
CCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCCCCCCC
VWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEE
WHADGSSTAVK
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]