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The map label for this gene is miaA

Identifier: 153937216

GI number: 153937216

Start: 1842690

End: 1843625

Strand: Reverse

Name: miaA

Synonym: CLC_1740

Alternate gene names: 153937216

Gene position: 1843625-1842690 (Counterclockwise)

Preceding gene: 153936560

Following gene: 153934601

Centisome position: 49.03

GC content: 28.53

Gene sequence:

>936_bases
ATGATAGATTTATTAATAATTGCAGGTCCTACAGCAGTAGGGAAAACAGATATTTCCATAAAACTTGCAGAAAAACTAAA
TGGAGAGATAATATCCGCTGACTCTATGCAAATTTATAAATACATGGATATAGGTTCAGCCAAAATAACTAAGGATGAAA
TGAAGGGAATACCTCACCATCTTATAGATGTAGTAGAACCCCATGAAGAATTCAATGTATCTTCCTTTAAAGCTCTAGCA
GAGAAATCTATAAAAGATATATGGAACAGAGGAAAGTTGCCCATAATAGCAGGAGGTACAGGGTTATATATTAATTCTTT
AATATACAATTATGATTTTACAGATGCAGATAGAGACGAAAAATATAGAGAATATCTTACTAAATTAGCAGAAGATAAAG
GGAAGGAATATGTTCATAGCCTGCTAAAAGATATAGATAAAGAATCCTATGAAAAGTTATATCCTAATGATCTAAAAAGA
GTAGTAAGAGCCTTAGAAGTTTACAAAATCACAGGTAAATCTATAAGCGAATACACAAAAGAAAACGAGAAAAAATTATA
TGATATCCCTTATAACGTAAACTACTTTATTTTAAATATGAATAGAGAGGTACTCTATGAAAGAATTAATAAAAGAGTGG
ATATAATGATGGGTAAAGGTTTAATAGAAGAAGTTAAAAAATTAGAGTCCATGGGATATACTCCAGATATGCAATCTATG
AAAGGTATAGGTTATAAAGAAGTTTTATTCTATTTAAATGGAGATATTTCACTAGATGAAGCTATTTATTTAATAAAAAA
AGGAAGCAGAAATTATGCTAAAAGACAACTTACTTGGTTTAGAAAAGATAAAAGATCTATATGGATAGATAAAGATAAAT
ATAGATCTGAGGAGGAAATAGTAGATAAAATTATAAAAATGGTAAAAGATAAATAG

Upstream 100 bases:

>100_bases
TCCATATACTTGTCCCCATGGAAGACCTACTATGATTAAATTTACATTAAAAGATTTAGAAAAAAAATTTAAAAGAATAC
AATGAGGGGGATAAGGAAAA

Downstream 100 bases:

>100_bases
AATTAAAAGGATTTTTAATTTATTTATAGAATTATATAAGAATAAGTTTCTAATTATTTGTAATTTTTTAGGAGGGAAAG
GTTATATGACTAAAGTTGTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDKESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYRSEEEIVDKIIKMVKDK

Sequences:

>Translated_311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDKESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYRSEEEIVDKIIKMVKDK
>Mature_311_residues
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDKESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYRSEEEIVDKIIKMVKDK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=31.8327974276527, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=35.2941176470588, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=33.2116788321168, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=35.6164383561644, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=34.4569288389513, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CLOB1 (A7FUK8)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384055.1
- ProteinModelPortal:   A7FUK8
- SMR:   A7FUK8
- STRING:   A7FUK8
- GeneID:   5394864
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1733
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   CBOT441770:CLB_1733-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36271; Mature: 36271

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCEEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKALAEKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGKEYVHSLLKDIDKESYEKLYPNDLKRVVRALEVYKITGKSISEYTK
HHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
HCCCEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYRSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEECHHHCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC
>Mature Secondary Structure
MIDLLIIAGPTAVGKTDISIKLAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCEEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKALAEKSIKDIWNRGKLPIIAGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGKEYVHSLLKDIDKESYEKLYPNDLKRVVRALEVYKITGKSISEYTK
HHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMGKGLIEEVKKLESMGYTPDMQSM
HCCCEEEECCCCCEEEEEECCHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYRSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEECHHHCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA