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The map label for this gene is truB [H]

Identifier: 153937079

GI number: 153937079

Start: 2390645

End: 2391514

Strand: Reverse

Name: truB [H]

Synonym: CLC_2262

Alternate gene names: 153937079

Gene position: 2391514-2390645 (Counterclockwise)

Preceding gene: 153934519

Following gene: 153936853

Centisome position: 63.59

GC content: 28.16

Gene sequence:

>870_bases
ATGGATGGAGTAATAAATGTTTTAAAACCAAAGGGGATAACATCCTTTGATGTAGTTAGAGACATAAGAAAAATAGCGAA
AATAAAAAAAGTCGGTCATACAGGTACATTAGATCCCTTAGCTTCTGGTGTTCTCCCTGTATGTATAGGTAAGGCTACCA
AAATAGTTGATTATATAATGGAAGGAACTAAAACCTACAGAGTAGAAATGAAACTTGGAACTACCACAGACACTTATGAT
AGAGAAGGTACTGTATTAGAGGAAAAAGAAATAGATCTAACTCCTATAGAGGTAGAAGAGACTATTAAAAAATATATAGG
AGACATAGATCAAGTACCTCCTATGTATTCTGCCTTAAAAATTAATGGACAAAAACTTTATGACTTGGCCAGAAAAGGTA
TAGAAGTAGAAAGAGAAGCAAGAAAAATACATATATATGATATATGTATTTTAAGTATAGATTTGCCCTATGTTATATTT
GATGTTAAGTGTTCAAAGGGAACTTATATAAGAAGTTTGTGTTTTGATATAGGAAAGGATTTAGATTCTGGAGCAGTAAT
GTGGAATCTACAAAGATTAGAAGCAAGCCCCTTTAATATAAAAGAAGCAATAAAATTAGAAGATTTAAATGAGGAAAATA
TAAAACAGTATATAACACCTATAGATGAAGCTTTAAAGGATTATGAAAAATTATCTTTAGATAAAAAATTTGAAAAACTT
GTACTAAATGGTGTAATATTAAAAGATAGAAGAGTTCTAGATAATATAGAAGAAAATAAACTATATAGAACTTATATAGA
AGATGATAATTTTATAGGATTAGGAATGAAAAATGCATATGGATTTAAAATTCATAAATTATTAACTTAG

Upstream 100 bases:

>100_bases
TGGAGGACATATTCGTGCTTCAGGAGCCTTTATAAAAGGAGAGGCTTTAAATATAGTAGAAAAAAAATTAATAGAAACTA
TAAAAAAAGAGTTGATATAA

Downstream 100 bases:

>100_bases
GAGTAATAGCTTATGATAGTTATGGAGGATAACTTTTCTACTAAATTAGAAGATAAAACTTATATAGCTTTAGGAAGTTT
TGATGGACTTCATAAAGGTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEIDLTPIEVEETIKKYIGDIDQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYVIF
DVKCSKGTYIRSLCFDIGKDLDSGAVMWNLQRLEASPFNIKEAIKLEDLNEENIKQYITPIDEALKDYEKLSLDKKFEKL
VLNGVILKDRRVLDNIEENKLYRTYIEDDNFIGLGMKNAYGFKIHKLLT

Sequences:

>Translated_289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEIDLTPIEVEETIKKYIGDIDQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYVIF
DVKCSKGTYIRSLCFDIGKDLDSGAVMWNLQRLEASPFNIKEAIKLEDLNEENIKQYITPIDEALKDYEKLSLDKKFEKL
VLNGVILKDRRVLDNIEENKLYRTYIEDDNFIGLGMKNAYGFKIHKLLT
>Mature_289_residues
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIMEGTKTYRVEMKLGTTTDTYD
REGTVLEEKEIDLTPIEVEETIKKYIGDIDQVPPMYSALKINGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYVIF
DVKCSKGTYIRSLCFDIGKDLDSGAVMWNLQRLEASPFNIKEAIKLEDLNEENIKQYITPIDEALKDYEKLSLDKKFEKL
VLNGVILKDRRVLDNIEENKLYRTYIEDDNFIGLGMKNAYGFKIHKLLT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=39.3805309734513, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI4503337, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI215599015, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367200, Length=288, Percent_Identity=35.7638888888889, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17553978, Length=192, Percent_Identity=30.7291666666667, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=145, Percent_Identity=39.3103448275862, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=244, Percent_Identity=26.6393442622951, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281364189, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364187, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364185, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281364183, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI62471759, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17975520, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32991; Mature: 32991

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGTKTYRVEMKLGTTTDTYDREGTVLEEKEIDLTPIEVEETIKKYIGDIDQVPPMYSALK
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHEE
INGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYVIFDVKCSKGTYIRSLCFDIGKD
ECCHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
LDSGAVMWNLQRLEASPFNIKEAIKLEDLNEENIKQYITPIDEALKDYEKLSLDKKFEKL
CCCCEEEEEEHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNGVILKDRRVLDNIEENKLYRTYIEDDNFIGLGMKNAYGFKIHKLLT
HHCCEEEECCHHHCCHHHCCEEEEEECCCCEEEECCCCCCCEEEEEECC
>Mature Secondary Structure
MDGVINVLKPKGITSFDVVRDIRKIAKIKKVGHTGTLDPLASGVLPVCIGKATKIVDYIM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGTKTYRVEMKLGTTTDTYDREGTVLEEKEIDLTPIEVEETIKKYIGDIDQVPPMYSALK
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHEE
INGQKLYDLARKGIEVEREARKIHIYDICILSIDLPYVIFDVKCSKGTYIRSLCFDIGKD
ECCHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCC
LDSGAVMWNLQRLEASPFNIKEAIKLEDLNEENIKQYITPIDEALKDYEKLSLDKKFEKL
CCCCEEEEEEHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNGVILKDRRVLDNIEENKLYRTYIEDDNFIGLGMKNAYGFKIHKLLT
HHCCEEEECCHHHCCHHHCCEEEEEECCCCEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA