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The map label for this gene is acoA [H]

Identifier: 153936990

GI number: 153936990

Start: 1739231

End: 1740214

Strand: Direct

Name: acoA [H]

Synonym: CLC_1656

Alternate gene names: 153936990

Gene position: 1739231-1740214 (Clockwise)

Preceding gene: 153935584

Following gene: 153937737

Centisome position: 46.25

GC content: 35.16

Gene sequence:

>984_bases
GTGAAAAAATTAAATGAAAATTCTATTGTTGAAATGTATAAAACTATGCTAAAAATAAGAAAGTTTGAGCAAGTGGCAAT
GAATACCTTTGCAGAAGGGAAAATTCCTGGCTTTGTTCATCTTTATATAGGAGAAGAAGCGGTGGCTACAGGAGTGTGTG
CTAATTTAAAGGATAGTGATTATATAACAAGTACTCACAGGGGTCATGGACATATACTTGCAAAGGGTGGAGATTTGAAA
TTTATGATGGCAGAGCTTTTTGGAAAGGCTACAGGATATTGCAAAGGAAAAGGAGGTTCTATGCATATTGCAGATGCAAC
TAAAGGTATTTTAGGGGCAAATGGTATAGTAGGTGCCGGTCATAATATTGCAGTGGGAGCTGGATTAAGTGCACAATACA
GAGGAACTGATCAAGTGTGTGTATGCTTCTTTGGAGATGCGTCAACTAACCAAGGTACATTTCATGAATCTTTAAATATG
GCAAGTGTATGGAAATTACCAGTGGTTTTTGTATGCGAAAATAACCTTTATGGAATATCTATGAGTCAAAATAGACATCA
AGCAATAAAGGATGTTGCAGATAGAGGTGTTGCTTATAATGTACCAGGTATTGTAGTAGATGGTAATGATGTATTTGCTG
TGTATGAAGCAGCTAAAGAAGCTATTAAAAGAGCAAGAGAAGGCAAGGGACCAACACTTATAGAATGCAAAACTTATCGA
CATAGAGGACATTTTGAAGGAGATCCTTGTGTTTACAAACCAACAGAGGAACAGGAAGAATGGCTAGCTAAAGATCCTAT
ACCAAGATTTGAAAAATATTTAGTAGAAAATGAAATTTTGACAGAAGAAAAGCTTAAAGAAGTTCAAAATAAGGTAGAGA
GTCAAATTGATGAAGCAGTAGATTTTGCAAATAATAGTCCATATCCAGAATTAGAGTCAGTATTAGAAGATGTTTATACT
GACATTAAAGAGGAGGTTAGATAA

Upstream 100 bases:

>100_bases
GAGTTTTTATTGCTGAATTTGGAGACTCAGATAATAGTATTGAAGTGGCGATTGCTTGTATTCAAGTATATATTAAATAA
AAAAACAGGAGGGAAAAACT

Downstream 100 bases:

>100_bases
CTATGAAAAAGTTAACATACGCAGAAGCAATAAGAGAAGCAATGAGAACTAAAATGAGTGAAGATGATAAGGTATTAATT
TTTGGGGAAGACGTAGGAGC

Product: TPP-dependent acetoin dehydrogenase complex, E1 component, alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKKLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYITSTHRGHGHILAKGGDLK
FMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHNIAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNM
ASVWKLPVVFVCENNLYGISMSQNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYR
HRGHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDFANNSPYPELESVLEDVYT
DIKEEVR

Sequences:

>Translated_327_residues
MKKLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYITSTHRGHGHILAKGGDLK
FMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHNIAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNM
ASVWKLPVVFVCENNLYGISMSQNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYR
HRGHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDFANNSPYPELESVLEDVYT
DIKEEVR
>Mature_327_residues
MKKLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSDYITSTHRGHGHILAKGGDLK
FMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAGHNIAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNM
ASVWKLPVVFVCENNLYGISMSQNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYR
HRGHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAVDFANNSPYPELESVLEDVYT
DIKEEVR

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=41.7445482866044, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI4505685, Length=321, Percent_Identity=39.2523364485981, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=39.2523364485981, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=38.4146341463415, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI291084757, Length=321, Percent_Identity=36.1370716510903, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI11386135, Length=326, Percent_Identity=29.1411042944785, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI258645172, Length=326, Percent_Identity=29.4478527607362, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32564172, Length=327, Percent_Identity=39.7553516819572, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17536047, Length=327, Percent_Identity=39.7553516819572, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=30.5810397553517, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=30.5810397553517, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=37.9310344827586, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24639744, Length=321, Percent_Identity=39.8753894080997, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28571106, Length=321, Percent_Identity=39.8753894080997, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=39.8753894080997, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24639748, Length=319, Percent_Identity=39.8119122257053, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=40.5844155844156, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21355903, Length=332, Percent_Identity=26.5060240963855, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36141; Mature: 36141

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCCCC
YITSTHRGHGHILAKGGDLKFMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAG
CEEECCCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCEEEECHHHCCCCCCCCCEECC
HNIAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGIS
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHEECCCEEEEECCCEEEEE
MSQNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYR
ECCCHHHHHHHHHHCCCEEECCEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCC
HRGHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAV
CCCCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFANNSPYPELESVLEDVYTDIKEEVR
HHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLNENSIVEMYKTMLKIRKFEQVAMNTFAEGKIPGFVHLYIGEEAVATGVCANLKDSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCCCC
YITSTHRGHGHILAKGGDLKFMMAELFGKATGYCKGKGGSMHIADATKGILGANGIVGAG
CEEECCCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCEEEECHHHCCCCCCCCCEECC
HNIAVGAGLSAQYRGTDQVCVCFFGDASTNQGTFHESLNMASVWKLPVVFVCENNLYGIS
CCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHEECCCEEEEECCCEEEEE
MSQNRHQAIKDVADRGVAYNVPGIVVDGNDVFAVYEAAKEAIKRAREGKGPTLIECKTYR
ECCCHHHHHHHHHHCCCEEECCEEEECCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCC
HRGHFEGDPCVYKPTEEQEEWLAKDPIPRFEKYLVENEILTEEKLKEVQNKVESQIDEAV
CCCCCCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFANNSPYPELESVLEDVYTDIKEEVR
HHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]