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The map label for this gene is psuK [H]

Identifier: 153936875

GI number: 153936875

Start: 1806080

End: 1807039

Strand: Direct

Name: psuK [H]

Synonym: CLC_1712

Alternate gene names: 153936875

Gene position: 1806080-1807039 (Clockwise)

Preceding gene: 153937802

Following gene: 153936177

Centisome position: 48.03

GC content: 28.54

Gene sequence:

>960_bases
ATGAATTATTATTGTAATAATGAACCATATGTTTTAGTTTTCGGAGCATCAGTAGTAGATATTTTTGGTTTTAGTTGTTG
CGATTATAGAGCTTACAATTCTAATCCGGGTAAAATAAAAATATCCTTTGGTGGAGTATGTAGAAATATAGCGGAAAATT
TATCAAGAGTTGGGATTAATACTAAATTTATATCTATATTAGGTGAGGATGAAAATGCTAAATGTATGTTAGAGCATTCA
AGAAAAATGGGATATGATATGGGAGACTCTCTTATTTTAAAAGGAGGTACAACCCCTACTTATATGGCCATATTAGATGA
AAATGGAGAAATGGTTTCAGCAGTGGCTGATATGAAAAGTATAGGCGAAATGAATTCCGAGTTTATAGATTCAAAGGCGG
ATATAATAAAAAAGGCAGAGTATACAGTTTTGGATTCAGATAATCCTGAAATATTAGAATATATATTAAAAAAGTTCAAA
GGAAAAACAAAGTTTATATTGGATCCTGTTTCTGCTGAAAAAGCTAAAAATATAAAGCCTTTAATAAAATATTTTCATAC
TATTAAACCTAATATACATGAAGCAGAAGTTTTATCAGGTTTCAAAATAGAAACTATAGAAGATTTAAAGAAAGCAGGAG
AGTATTTTGAATCTTTAGGTGTAGAAAATGTTTTTATAAGTTTAGACGCAGAAGGAATCTATTATAAAAATAAATTTGAA
GAGGGAAGAATAAAGGCTAAGGATGTTACAGTCAAAAATGTTACAGGAGCAGGAGATGCCTTTGTAGCAGGTATAGCATA
TGGTTATATGAATAAATTATCTTTAGAGGAAACTGTAAAGTTTGCTATAACTATGGCTAATTTAACTATAGCTCACGAAA
ACACTATAAATCCAGATTTAAGTTATAATATTGTAGAGAATAATATAAAAAATATTCAATGGATTGAAGAATACTTATAA

Upstream 100 bases:

>100_bases
GAATATTGGAGGAAGTAGAAAAGCTATTAGAAATAAAAAAACTGGTGGATTATTTGATAAATAGATAATATTTAGATTTA
TATTGTGGAGGTTACTAAGG

Downstream 100 bases:

>100_bases
TTAAATCAGATAATATAGGTAATTATAAGTATAAGAAATATAATACATAAAATGATTACGTAACAAAGTACTAAAAATAA
ATTTCATTTTAACAATAAAA

Product: PfkB family kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNYYCNNEPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSEFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKNIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLKKAGEYFESLGVENVFISLDAEGIYYKNKFE
EGRIKAKDVTVKNVTGAGDAFVAGIAYGYMNKLSLEETVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL

Sequences:

>Translated_319_residues
MNYYCNNEPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSEFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKNIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLKKAGEYFESLGVENVFISLDAEGIYYKNKFE
EGRIKAKDVTVKNVTGAGDAFVAGIAYGYMNKLSLEETVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL
>Mature_319_residues
MNYYCNNEPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGINTKFISILGEDENAKCMLEHS
RKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKSIGEMNSEFIDSKADIIKKAEYTVLDSDNPEILEYILKKFK
GKTKFILDPVSAEKAKNIKPLIKYFHTIKPNIHEAEVLSGFKIETIEDLKKAGEYFESLGVENVFISLDAEGIYYKNKFE
EGRIKAKDVTVKNVTGAGDAFVAGIAYGYMNKLSLEETVKFAITMANLTIAHENTINPDLSYNIVENNIKNIQWIEEYL

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=268, Percent_Identity=24.6268656716418, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788491, Length=304, Percent_Identity=34.5394736842105, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788484, Length=301, Percent_Identity=29.9003322259136, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790193, Length=242, Percent_Identity=23.9669421487603, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081971, Length=324, Percent_Identity=24.3827160493827, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788493, Length=126, Percent_Identity=31.7460317460317, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25147470, Length=330, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 35715; Mature: 35715

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYCNNEPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGIN
CCCEECCCCEEEEECCHHHHHHCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHCCCC
TKFISILGEDENAKCMLEHSRKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKS
EEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHH
IGEMNSEFIDSKADIIKKAEYTVLDSDNPEILEYILKKFKGKTKFILDPVSAEKAKNIKP
HHHHHHHHHCCHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCHHH
LIKYFHTIKPNIHEAEVLSGFKIETIEDLKKAGEYFESLGVENVFISLDAEGIYYKNKFE
HHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
EGRIKAKDVTVKNVTGAGDAFVAGIAYGYMNKLSLEETVKFAITMANLTIAHENTINPDL
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEEEEEEEEEECCCCCCCC
SYNIVENNIKNIQWIEEYL
EEEEHHCCCCHHHHHHHCC
>Mature Secondary Structure
MNYYCNNEPYVLVFGASVVDIFGFSCCDYRAYNSNPGKIKISFGGVCRNIAENLSRVGIN
CCCEECCCCEEEEECCHHHHHHCCCCCCCEEECCCCCEEEEEECHHHHHHHHHHHHCCCC
TKFISILGEDENAKCMLEHSRKMGYDMGDSLILKGGTTPTYMAILDENGEMVSAVADMKS
EEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHH
IGEMNSEFIDSKADIIKKAEYTVLDSDNPEILEYILKKFKGKTKFILDPVSAEKAKNIKP
HHHHHHHHHCCHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCHHH
LIKYFHTIKPNIHEAEVLSGFKIETIEDLKKAGEYFESLGVENVFISLDAEGIYYKNKFE
HHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
EGRIKAKDVTVKNVTGAGDAFVAGIAYGYMNKLSLEETVKFAITMANLTIAHENTINPDL
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHEEEEEEEEEEEEECCCCCCCC
SYNIVENNIKNIQWIEEYL
EEEEHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]