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The map label for this gene is rimO

Identifier: 153935820

GI number: 153935820

Start: 2378769

End: 2380106

Strand: Reverse

Name: rimO

Synonym: CLC_2253

Alternate gene names: 153935820

Gene position: 2380106-2378769 (Counterclockwise)

Preceding gene: 153936780

Following gene: 153935033

Centisome position: 63.29

GC content: 28.18

Gene sequence:

>1338_bases
GTGGAAAAAATTAAAGTAGCATTAGTTAGCTTAGGTTGTGATAAAAATAGAATTGATTCAGAACTTATGCTATACAAATT
AAATGAAGAAGCAGAACTAGTAAAAGATCCTAAAGAAGCACAGGTAATTATAGTAAATACCTGTGGATTTATTGAGACCG
CTAAGGAAGAATCTATAAATACCATTTTACAAATGGCTTCCTATAAAAAAACTCATAATTGCAAAGTATTAGTAGTTACA
GGATGTTTGACTCAAAGATATAAAGGGGAACTTAAAGAACTTATCCCAGAAATGGATATTATGTTAGGGGTCAATGATTA
TGATAAGTTACTTGAAAGTATAAAAGTTTTTTTGAAATCAGGAGAAAAAAGTTTTTATCATAAATATAGTGATACAAAAA
TAAATGAGGGAAATAGAATATTAACTACGCCAACATACACAGCCTATGTGAGAATAGCAGAAGGATGTAATAATTTTTGT
ACTTACTGTGCCATACCACGCATAAGAGGAAAGTATAGAAGTAGAAAAAAAGAAAATATATTAAAGGAAGTAGAAAATTT
AGCTAAACAAGGAGTTAAAGAAATAATATTGATAGCTCAAGATACAACCATGTATGGAATAGATATATATGGCAAAAAAG
TTTTACATGAGCTTCTAAGAGATATTTCTAAAGTTGAAGGTGTAAAGTGGATAAGATTATTATACTGTTACCCAGAGGAA
ATAACTAAGGAACTTATAGAAGAGATTAAAAATAATGATAAGGTATGTAAATATTTAGATTTGCCTATACAGCAGATAAG
TAATTCTGTTTTAAAAAGAATGGGAAGAAAAACTACAAAGGAAACTATCATAAATATAATAAAAAAATTAAGAAAAGAAA
TAGAGGGTATTACTTTAAGGACTTCATTAATAGTAGGATTTCCAGGGGAAACAGAAGGAGAGTTTAGTGAACTTAAAGAA
TTTGTTTCAGATGTAAAATTAGATAAATTAGGTGTATTTAAATATTCAAAGGAAGAAGGAACGTCAGCAGCTCTTATGGA
GGAACAAATAGATGAAGAAATAAAAGAAAAAAGAGAAGAAGAAATAATGATACTTCAGCAAAGTATATCTAAAGACATAA
ATAAAGAGAAAATAGGTAAAACATATGAAGTCATAGTAGAAGGTACCAAAGAAGATATGTATTATGGGAGAAATTATGAA
ATGTCACCGGAGATAGATGGAGAAATTTACTTTGAAAAGGATGAAAACGTAAAAATTGGTGATATAATAAAGGTGAAGGT
AACTCATAGTTTAGAATATGATTTAATAGGAGTTGTTTATAATGAACTTAGCAAATAA

Upstream 100 bases:

>100_bases
ATGTAAATACTTTTAAAATTCAAGTTACTTAATACTTGTAAAATTTCTTAAGTAGATTTAAAATGTTTCTTGTGACATAA
AAAATGTAGGAGGGGAACAG

Downstream 100 bases:

>100_bases
ACTAACTATATTAAGAATTTTTTTAGTTCCAATATTTCTTATATTTATATCAGTAAAAAACATACCCTATGGTACAGTAA
TAGCTACTGCAGTTTTCGTA

Product: RNA modification protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKDPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKTYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK

Sequences:

>Translated_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKDPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKTYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK
>Mature_445_residues
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKDPKEAQVIIVNTCGFIETAKEESINTILQMASYKKTHNCKVLVVT
GCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKSGEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFC
TYCAIPRIRGKYRSRKKENILKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLRTSLIVGFPGETEGEFSELKE
FVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREEEIMILQQSISKDINKEKIGKTYEVIVEGTKEDMYYGRNYE
MSPEIDGEIYFEKDENVKIGDIIKVKVTHSLEYDLIGVVYNELSK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=28.0737704918033, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=28.2744282744283, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI93277076, Length=392, Percent_Identity=27.0408163265306, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1787057, Length=444, Percent_Identity=38.0630630630631, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1786882, Length=425, Percent_Identity=30.5882352941176, Blast_Score=191, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=24.7357293868922, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=27.7628032345013, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=26.5957446808511, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=25.7709251101322, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CLOB1 (A7FVY1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384581.1
- STRING:   A7FVY1
- GeneID:   5395775
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2270
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK882944
- BioCyc:   CBOT441770:CLB_2270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51351; Mature: 51351

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKDPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKTYEVIVEGTKEDMYYGRNYEMSPEIDGEIYFEKDENVKIG
HHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHEECCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MEKIKVALVSLGCDKNRIDSELMLYKLNEEAELVKDPKEAQVIIVNTCGFIETAKEESIN
CCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TILQMASYKKTHNCKVLVVTGCLTQRYKGELKELIPEMDIMLGVNDYDKLLESIKVFLKS
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHC
GEKSFYHKYSDTKINEGNRILTTPTYTAYVRIAEGCNNFCTYCAIPRIRGKYRSRKKENI
CCHHHHHCCCCCEECCCCEEEECCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
LKEVENLAKQGVKEIILIAQDTTMYGIDIYGKKVLHELLRDISKVEGVKWIRLLYCYPEE
HHHHHHHHHHCHHHEEEEEECCEEEEEEHHHHHHHHHHHHHHHHHCCHHEEHHEEECCHH
ITKELIEEIKNNDKVCKYLDLPIQQISNSVLKRMGRKTTKETIINIIKKLRKEIEGITLR
HHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEE
TSLIVGFPGETEGEFSELKEFVSDVKLDKLGVFKYSKEEGTSAALMEEQIDEEIKEKREE
EEEEEECCCCCCCCHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EIMILQQSISKDINKEKIGKTYEVIVEGTKEDMYYGRNYEMSPEIDGEIYFEKDENVKIG
HHHHHHHHHHHCCCHHHCCCEEEEEEECCHHHHEECCCCCCCCCCCCEEEEECCCCEEEC
DIIKVKVTHSLEYDLIGVVYNELSK
CEEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA