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The map label for this gene is pnpA

Identifier: 153934955

GI number: 153934955

Start: 2386944

End: 2389052

Strand: Reverse

Name: pnpA

Synonym: CLC_2259

Alternate gene names: 153934955

Gene position: 2389052-2386944 (Counterclockwise)

Preceding gene: 153935901

Following gene: 153937094

Centisome position: 63.53

GC content: 33.24

Gene sequence:

>2109_bases
ATGATTCATACATTAGAAACTACTGTAGCAGGAAGAAAAATGAAAGTAGACTTTGGTAAGACAGGAATGCTTTCAAATGC
TGCCATATTCATGAGTTATGGAGATACAGTAGTTATGATAAATGCCAACGCTTCTAAAGAGCCAAGAGAGGGAATAGATT
TTTTCCCATTAAGCGTTGAATATGAAGAAAGACTATATTCAGTAGGAAAGATTCCAGGAGGATTCATCAAAAGAGAAGGA
AAACCTTCTGACAAATCTATATTACATGCCAGATCTATAGATAGACCATTAAGACCTTTATTCCCAAAGGGATACAGAAA
TGATGTTCAAATAGTTAATACAGTTTTATCTGTAGAACAGGATAATTTACCAGAAATATTAGCTATAAATGGCTCCTCTT
TAGCTCTATGCCTATCTAGCATACCATTTACAACACCAGTAGCTGCAGTTTCTGTGGGATTAGTAGATGGAGAATTTATA
ATAAATCCAACTGTAGCCCAAAGAGAAAATACTATATTAGATTTAACTGTATGCGCTACAAAAGAAAGAGTTATGATGGT
AGAAGCAGGTGGACAGGAAATAGATGAGGAAACTATGTATAGTGCTATCATGTTTGGTTTTGAAGAATGCAAAAATATAG
TTGCATTCCAAGAAGAGGCTGTGGCTAAGTTTGGAAAAACTAAAAATGAACCAGTATTATACAAAGCTGACGAAGAAGTA
GAAAAAGAAGTAAAAAGCTTTGCTTTTGATATGATAAAAGAAGCTATGTATATAATGGATAAAGATGAAAGAAATGCTCA
GTTAGATAAAGTTAAAGAAAAAATATCAGAAGAGTTTTCAGAAAAATATGAAGACAAAGTGGCAGACATAGCAGAAGTTA
TATATAAAACTCAAAAAGAAATAGTTAGAAACATGCTTTTAAATGAAGATAGAAGACCAGATGGAAGAGCTTTTGATGAA
GTAAGACCTATAAGCTGTGAAGTGGGAATACTTCCAAGAACTCATGGTACGGGTTTATTTACAAGAGGATTAACTCAAGT
AATGACTGTAGCTACTTTAGGAGCTTTAGGAGATGTTCAAATATTAGATGGTATAGCAGAGGAAGAATCAAAACGCTACA
TGCATCATTACAATTTCCCATCCTATAGTGTAGGAGAAGTTAGACCTTTAAGGGGACCTGGAAGACGTGAAATAGGTCAT
GGAGCTTTAGCAGAAAGAGCTTTGGAACCATTAATTCCATCTCAATCAGAGTTCCCATACACTATAAGATTAGTATCAGA
AGTACTAAGTTCAAATGGTTCAACATCACAGGCAAGTGTTTGCGGAAGCACATTAGCTCTATTAGATGCAGGTGTACCTA
TAAAAAGGCCAGCCGCAGGTATAGCTATGGGACTTATAACTAGTGAAGATTTAGAAAAGGAAAAAGTAATAACAGACATT
CAAGGTATAGAAGATTTCTTTGGAGATATGGACTTTAAGGTAGCAGGAACAGAAAAAGGGATTACATCTATACAATTTGA
TACAAAGATAAAAGGGTTATCAAATAGTTGTGTAAAGGATGCATTAGAAGGCGCAAAAAAGGCAAGACTACACATATTAG
GAAAAATAAAAGAATGCATACCAGAACCAAGAAAAGAATTATCTAAATATGCACCAAGAACAGAAATAATATGTATAGAT
CCAGAAAAAATAAGAGATGTTATAGGTGCTGGTGGAAAGGTAATAAATAAAATAATAGCAGATACCAACGTAAAAATAGA
AATAAAAGAAGATGGAAAAATATTTGTAACATCTAATAATGAGCCTGAAGGTGTTAAGAAAGCTATAAGTATAATAGAAG
GATTAACAAAGGAAGTAGTTCAAGGAGAGATTTATCTAGGTAAAGTAACTAAAACTACTAATTTTGGAGCCTTTGTAGAA
ATACTGCCAGGTAAAGAAGGGCTAGTTCATATATCTAAATTAGATTTTGCTAGAGTAGAAAAAGTGGAAGATGTAGTATC
TGTAGGAGATGAAATATTAGTTAAGGTTACAGACATAGATAATCAAGGAAGAATAAATTTATCACGCAAAGATGCTATTG
CCAAGAAAGAAGAAGAAAAAGATAAATAA

Upstream 100 bases:

>100_bases
ATTAATAGGTTATAAACAAATATAAAAATTAAATGTTTGCTATAAATGTAATAGTGATATAATGATAATATTAGTTCAAT
TATTGAAGGGAGGTTAATGT

Downstream 100 bases:

>100_bases
TAAAAAATAGAAGGGCATATCTGCCTTTTTATTTTTTATGAAGTGATTCTTAGGTTCAGGTGGAAGTTACTTATACAGAG
TGCTGATTTGGTGATAATCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKVADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQSEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Sequences:

>Translated_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKVADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQSEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
>Mature_702_residues
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVEYEERLYSVGKIPGGFIKREG
KPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQDNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFI
INPTVAQRENTILDLTVCATKERVMMVEAGGQEIDEETMYSAIMFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKVADIAEVIYKTQKEIVRNMLLNEDRRPDGRAFDE
VRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGH
GALAERALEPLIPSQSEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECIPEPRKELSKYAPRTEIICID
PEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNNEPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVE
ILPGKEGLVHISKLDFARVEKVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=36.7897727272727, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=44.8680351906158, Blast_Score=590, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI115534063, Length=722, Percent_Identity=32.9639889196676, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI281362905, Length=704, Percent_Identity=38.3522727272727, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=704, Percent_Identity=38.3522727272727, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=704, Percent_Identity=38.3522727272727, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=38.828967642527, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CLOB1 (A7FVY7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384587.1
- ProteinModelPortal:   A7FVY7
- STRING:   A7FVY7
- GeneID:   5397699
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2276
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   CBOT441770:CLB_2276-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77455; Mature: 77455

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVE
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEEEEE
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIMFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKVADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRNMLLNEDRRPDGRAFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSQSEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC
>Mature Secondary Structure
MIHTLETTVAGRKMKVDFGKTGMLSNAAIFMSYGDTVVMINANASKEPREGIDFFPLSVE
CCEEECHHHCCCEEEECCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCCEEEEEEE
YEERLYSVGKIPGGFIKREGKPSDKSILHARSIDRPLRPLFPKGYRNDVQIVNTVLSVEQ
HHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DNLPEILAINGSSLALCLSSIPFTTPVAAVSVGLVDGEFIINPTVAQRENTILDLTVCAT
CCCCCEEEECCCHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHCCCCCEEEEEEECC
KERVMMVEAGGQEIDEETMYSAIMFGFEECKNIVAFQEEAVAKFGKTKNEPVLYKADEEV
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHH
EKEVKSFAFDMIKEAMYIMDKDERNAQLDKVKEKISEEFSEKYEDKVADIAEVIYKTQKE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVRNMLLNEDRRPDGRAFDEVRPISCEVGILPRTHGTGLFTRGLTQVMTVATLGALGDVQ
HHHHHHHCCCCCCCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHH
ILDGIAEEESKRYMHHYNFPSYSVGEVRPLRGPGRREIGHGALAERALEPLIPSQSEFPY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
TIRLVSEVLSSNGSTSQASVCGSTLALLDAGVPIKRPAAGIAMGLITSEDLEKEKVITDI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
QGIEDFFGDMDFKVAGTEKGITSIQFDTKIKGLSNSCVKDALEGAKKARLHILGKIKECI
HHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PEPRKELSKYAPRTEIICIDPEKIRDVIGAGGKVINKIIADTNVKIEIKEDGKIFVTSNN
CCHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCEEEEECCC
EPEGVKKAISIIEGLTKEVVQGEIYLGKVTKTTNFGAFVEILPGKEGLVHISKLDFARVE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEHHHHHHHH
KVEDVVSVGDEILVKVTDIDNQGRINLSRKDAIAKKEEEKDK
HHHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA