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The map label for this gene is def-3 [H]

Identifier: 153933577

GI number: 153933577

Start: 2684762

End: 2685298

Strand: Reverse

Name: def-3 [H]

Synonym: CLB_2564

Alternate gene names: 153933577

Gene position: 2685298-2684762 (Counterclockwise)

Preceding gene: 153931110

Following gene: 153932076

Centisome position: 69.51

GC content: 30.35

Gene sequence:

>537_bases
ATGTATAATTTAGTTATACATGATAAAAAGGAGGGAGTTTTAAAGCTTTTAGCTTTAAGAATTATTATGGCAATAAGAGA
GATATTACAAGTTGGAGACAAGACGTTGAAAAGAGTTAGTAAAAAAGTAGAATGTATTGATGATGAAATTACAGGTATTA
TAAAAGATTTGAAGGATACTTTATATGCAGGTACTGGTATTGGTCTTGCAGCACCTCAAATAGGTTATTTAAAAAGAATT
TTCATTATAGATTTAAGAAATGGACAAGAACCTATAATTCTTATAAATCCTAAATTTTCAAAAAAAATAGGAAAAGAAGA
AAGTGAAGAGGGGTGCTTAAGCTATCCAGGTTATGAAGGGATTGTTATTAGACCCAGAAGAGTTGCTATAACGGGACTAA
ATGAGAAAGGTGAAGAAGTTACTTATGAAGCAACAGGCTTACTAAAGAATGCTTTTTGCCATGAGTATGATCATTTAGAT
GGCATAGTTTATATAGATAAGGCTAAGAAGGTATACAAAGTGGAACAAATAGAATAA

Upstream 100 bases:

>100_bases
TATTAAAGTAAGCATTTTAATTTTAGAGTAAAAGTATAAGGAAAGATGAATTTCTAAATTCTGTATTTCTTATAATAATC
TCGCATTTTTCTAGAATATT

Downstream 100 bases:

>100_bases
AAAAGGGGAGAATGCTAATGCATAGGATTATTTATGACTGTGATAACACCATGGGGCTTAAAGATAAAGACGTGGATGAT
GGTCTAACTTTAATGTATCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MYNLVIHDKKEGVLKLLALRIIMAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRI
FIIDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLD
GIVYIDKAKKVYKVEQIE

Sequences:

>Translated_178_residues
MYNLVIHDKKEGVLKLLALRIIMAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRI
FIIDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLD
GIVYIDKAKKVYKVEQIE
>Mature_178_residues
MYNLVIHDKKEGVLKLLALRIIMAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDTLYAGTGIGLAAPQIGYLKRI
FIIDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEGIVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLD
GIVYIDKAKKVYKVEQIE

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI1789682, Length=145, Percent_Identity=40.6896551724138, Blast_Score=111, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=31.5151515151515, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20091; Mature: 20091

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLVIHDKKEGVLKLLALRIIMAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDT
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAGTGIGLAAPQIGYLKRIFIIDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEG
HHCCCCCCCCCCCHHHEEEEEEEEECCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCC
IVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE
EEECCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC
>Mature Secondary Structure
MYNLVIHDKKEGVLKLLALRIIMAIREILQVGDKTLKRVSKKVECIDDEITGIIKDLKDT
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAGTGIGLAAPQIGYLKRIFIIDLRNGQEPIILINPKFSKKIGKEESEEGCLSYPGYEG
HHCCCCCCCCCCCHHHEEEEEEEEECCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCC
IVIRPRRVAITGLNEKGEEVTYEATGLLKNAFCHEYDHLDGIVYIDKAKKVYKVEQIE
EEECCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]