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The map label for this gene is ygfA [C]

Identifier: 153933071

GI number: 153933071

Start: 2669805

End: 2670359

Strand: Reverse

Name: ygfA [C]

Synonym: CLB_2548

Alternate gene names: 153933071

Gene position: 2670359-2669805 (Counterclockwise)

Preceding gene: 153933890

Following gene: 153932366

Centisome position: 69.12

GC content: 26.49

Gene sequence:

>555_bases
ATGAATAATAAAGCTATTTTAAGAAAAGAAATTATAAATGTAAGGAAATCTTTAACTTTAGAAGAAAAAATAAAAAGAGA
TATAATAATAAAAGATAAGCTCCAGAAAAATTCTTTGTATAAGGAATCTAATTTCATATTTATATACGTAAGTTTAAAAG
ATGAAGTGGACACACATGCTATTATAAAAGAGGGATTAAAGCATGGGAAAAAAATATGTGTGCCTAAGGTTATATCTATA
CAAGAAGGTATGGTGGCTATAGAAATAAAGGATTTTTCAGAACTTATAGAATGTGGACCTTATAAAATATTAGAACCTAA
AAATTTTAAAAATATAGTAGATTCAAAAGAAATAGATCTAGCTATTTTACCAGGTTTAGCTTTTGATAACAAAGGGGGGA
GATTAGGTTATGGTGGAGGCTTTTATGATAGGTTTATTCCTAAACTAAAAAAAGAAGTTTCAAAAATAGCCTTAGCCTAT
GATTTTCAAATATTAAAAGAGGTTCCTAAAGATGCACATGATGTGCTAGTAGATGACATTATAATAGATATTTAA

Upstream 100 bases:

>100_bases
GCTGCAGCAGCAAATTATAGATATAATTTAGTTACAAAAAATTGCCCAGCAGATATAATAAATGAAAGAATTAGTAAGGA
GGCTGTTAGCTCAATAGAAG

Downstream 100 bases:

>100_bases
TATTTATAAACAGAGTTCTTGGCCTTAGAGGGAGTTTTTACTCCCACTGAAGCTTATTAACAGAATTCTTAGGAGTTTTA
CTCCTTAGAAGTCGTTATCC

Product: 5-formyltetrahydrofolate cyclo-ligase family protein

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNNKAILRKEIINVRKSLTLEEKIKRDIIIKDKLQKNSLYKESNFIFIYVSLKDEVDTHAIIKEGLKHGKKICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDNKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDDIIIDI

Sequences:

>Translated_184_residues
MNNKAILRKEIINVRKSLTLEEKIKRDIIIKDKLQKNSLYKESNFIFIYVSLKDEVDTHAIIKEGLKHGKKICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDNKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDDIIIDI
>Mature_184_residues
MNNKAILRKEIINVRKSLTLEEKIKRDIIIKDKLQKNSLYKESNFIFIYVSLKDEVDTHAIIKEGLKHGKKICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDNKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDDIIIDI

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=191, Percent_Identity=29.8429319371728, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1789278, Length=170, Percent_Identity=25.2941176470588, Blast_Score=73, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17510857, Length=190, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321031, Length=203, Percent_Identity=27.0935960591133, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI161077430, Length=192, Percent_Identity=27.6041666666667, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 20988; Mature: 20988

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKAILRKEIINVRKSLTLEEKIKRDIIIKDKLQKNSLYKESNFIFIYVSLKDEVDTHA
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCEECCCEEEEEEEECCCCHHHH
IIKEGLKHGKKICVPKVISIQEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDL
HHHHHHHCCCEECCCHHEECCCCEEEEEEHHHHHHHHCCCCEEECCCCHHHHCCCCCCCE
AILPGLAFDNKGGRLGYGGGFYDRFIPKLKKEVSKIALAYDFQILKEVPKDAHDVLVDDI
EEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIDI
HCCC
>Mature Secondary Structure
MNNKAILRKEIINVRKSLTLEEKIKRDIIIKDKLQKNSLYKESNFIFIYVSLKDEVDTHA
CCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCEECCCEEEEEEEECCCCHHHH
IIKEGLKHGKKICVPKVISIQEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDL
HHHHHHHCCCEECCCHHEECCCCEEEEEEHHHHHHHHCCCCEEECCCCHHHHCCCCCCCE
AILPGLAFDNKGGRLGYGGGFYDRFIPKLKKEVSKIALAYDFQILKEVPKDAHDVLVDDI
EEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIDI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]